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The map label for this gene is alaXL [H]

Identifier: 239617886

GI number: 239617886

Start: 1624257

End: 1625390

Strand: Direct

Name: alaXL [H]

Synonym: Kole_1514

Alternate gene names: 239617886

Gene position: 1624257-1625390 (Clockwise)

Preceding gene: 239617879

Following gene: 239617887

Centisome position: 70.55

GC content: 38.1

Gene sequence:

>1134_bases
GTGAAACAAAGGATTCTGATGACGTTTGATAAAAAGGATTTTTCTTTGTTATGCTTCGAAAAAGGGACATTGTATGTCGA
TGGTGAAGGTGGACAACTGGGGGATAGGGGGAAAATAGATGGTAATAAAATCCTTCAGGTTAAGAGAGTAGATGGATATC
CTTGTGCCGTGATAGAGAAGAAAAATAATTACAGTACTGGTGAATTTGTTGAATTAGAATTGGATTTAGAGAGAAGGGTC
GATATTGCTCAACAGCATACCGCACAACACCTGTTATCTGCAGTTGCACTTCGTGAACTTGATGCTGAAACAGTTGGGTT
TCAAATGGGCGAAGAGTTTTCCACAATAGATCTCACCATCGGTTTGCTGGAGGATAAGATGAAACGTTTACTCGAAGATG
TTTCTAACGATTGCATTTTGAAACTTCTTCCAGTGACGGTAAAAGAGGTAGATCCTTCGGAGATCTTTCGATACAACCTT
AGAAAATCTGTGAGTGATAAGATACTGGAAAAGGCTGAAAAAATCAGGCTGGTGAAGATTGAGGGAATTGACGTAAGTCC
ATGTGGCGGATTTCATGTGAAGAATACAGGCGAGGTCGGGTTGCTTAAAATCCTTAAGGTAGAAAAGGTAAAAGGAAATC
TTACGAGAGTATATTTTGTTGCCGGAAAGAGGGCTATCAGGGTTTTTCAAAATGAACATTCAATTCTAGGATCTCTTGGA
AAACTTCTGACTTGTGGTTATCATGAACTGGTATCACGGACTCAATCAATTCTTGAAGATCTGAAAATTATGAACAACAG
GATAAAAGGATATGTAGAGAGGATCGCTAAAGAAACAGCTAATAATTTATCAGAGAATAGTTCAGAACTTTTACTTTATG
AAGACGATGAAGAGATACTTTCAATGATTCCACGTTTTCTCGATAAAGAGTGTTATATTTTCTTTGGTAAAGCAGGTGAA
AAGATTATCTTAGCCTCAAAGGGTTACGACCTTACAATTCTTGTCAAAGAGTTAAAAAGCCGGTTTGATATAAAAGGCGG
AGCTGGTAAAACCAGAGGGCAGTTTGTCTATTCGAAAGAGCTTGATGAGCTTATTGAATTTACCCTTCACTGGATGAAAG
GAGAGAAATCATGA

Upstream 100 bases:

>100_bases
TCATTACAGATTTTGGTTTGAAAAGAATTCCCCCGGAACAATTTACAGTATAATTATTACATAGAAAGGCATCAAATATC
AAGTGGTGGGGGCGAGATGA

Downstream 100 bases:

>100_bases
ATCCGAAGATACCACCACATAGCAACGAAGCAGAGGAGGCAGTTATAGGTAGCATACTCATAGACCCTGATGTCATTCCA
GATGTTATGGAGATCCTCAG

Product: Threonyl/alanyl tRNA synthetase SAD

Products: AMP; diphosphate; L-alanyl-tRNAAla [C]

Alternate protein names: AlaX-L; Alanyl-tRNA deacylase AlaX-L [H]

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MKQRILMTFDKKDFSLLCFEKGTLYVDGEGGQLGDRGKIDGNKILQVKRVDGYPCAVIEKKNNYSTGEFVELELDLERRV
DIAQQHTAQHLLSAVALRELDAETVGFQMGEEFSTIDLTIGLLEDKMKRLLEDVSNDCILKLLPVTVKEVDPSEIFRYNL
RKSVSDKILEKAEKIRLVKIEGIDVSPCGGFHVKNTGEVGLLKILKVEKVKGNLTRVYFVAGKRAIRVFQNEHSILGSLG
KLLTCGYHELVSRTQSILEDLKIMNNRIKGYVERIAKETANNLSENSSELLLYEDDEEILSMIPRFLDKECYIFFGKAGE
KIILASKGYDLTILVKELKSRFDIKGGAGKTRGQFVYSKELDELIEFTLHWMKGEKS

Sequences:

>Translated_377_residues
MKQRILMTFDKKDFSLLCFEKGTLYVDGEGGQLGDRGKIDGNKILQVKRVDGYPCAVIEKKNNYSTGEFVELELDLERRV
DIAQQHTAQHLLSAVALRELDAETVGFQMGEEFSTIDLTIGLLEDKMKRLLEDVSNDCILKLLPVTVKEVDPSEIFRYNL
RKSVSDKILEKAEKIRLVKIEGIDVSPCGGFHVKNTGEVGLLKILKVEKVKGNLTRVYFVAGKRAIRVFQNEHSILGSLG
KLLTCGYHELVSRTQSILEDLKIMNNRIKGYVERIAKETANNLSENSSELLLYEDDEEILSMIPRFLDKECYIFFGKAGE
KIILASKGYDLTILVKELKSRFDIKGGAGKTRGQFVYSKELDELIEFTLHWMKGEKS
>Mature_377_residues
MKQRILMTFDKKDFSLLCFEKGTLYVDGEGGQLGDRGKIDGNKILQVKRVDGYPCAVIEKKNNYSTGEFVELELDLERRV
DIAQQHTAQHLLSAVALRELDAETVGFQMGEEFSTIDLTIGLLEDKMKRLLEDVSNDCILKLLPVTVKEVDPSEIFRYNL
RKSVSDKILEKAEKIRLVKIEGIDVSPCGGFHVKNTGEVGLLKILKVEKVKGNLTRVYFVAGKRAIRVFQNEHSILGSLG
KLLTCGYHELVSRTQSILEDLKIMNNRIKGYVERIAKETANNLSENSSELLLYEDDEEILSMIPRFLDKECYIFFGKAGE
KIILASKGYDLTILVKELKSRFDIKGGAGKTRGQFVYSKELDELIEFTLHWMKGEKS

Specific function: Functions in trans to edit the amino acid moiety from mischarged charged tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-L subfamily [H]

Homologues:

Organism=Homo sapiens, GI13376886, Length=355, Percent_Identity=28.4507042253521, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI217416402, Length=356, Percent_Identity=28.3707865168539, Blast_Score=119, Evalue=5e-27,
Organism=Escherichia coli, GI1789048, Length=298, Percent_Identity=23.8255033557047, Blast_Score=76, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324288, Length=295, Percent_Identity=28.8135593220339, Blast_Score=95, Evalue=1e-20,
Organism=Drosophila melanogaster, GI18543305, Length=279, Percent_Identity=30.4659498207885, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7 [C]

Molecular weight: Translated: 42743; Mature: 42743

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQRILMTFDKKDFSLLCFEKGTLYVDGEGGQLGDRGKIDGNKILQVKRVDGYPCAVIEK
CCCCEEEEECCCCCEEEEEECCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEC
KNNYSTGEFVELELDLERRVDIAQQHTAQHLLSAVALRELDAETVGFQMGEEFSTIDLTI
CCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHCCCCCCEEEEEE
GLLEDKMKRLLEDVSNDCILKLLPVTVKEVDPSEIFRYNLRKSVSDKILEKAEKIRLVKI
HHHHHHHHHHHHHCCCCEEEEEECCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEE
EGIDVSPCGGFHVKNTGEVGLLKILKVEKVKGNLTRVYFVAGKRAIRVFQNEHSILGSLG
ECEEECCCCCEEECCCCCCHHEEEEEHHHCCCCEEEEEEEECCCEEEHHHHHHHHHHHHH
KLLTCGYHELVSRTQSILEDLKIMNNRIKGYVERIAKETANNLSENSSELLLYEDDEEIL
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHH
SMIPRFLDKECYIFFGKAGEKIILASKGYDLTILVKELKSRFDIKGGAGKTRGQFVYSKE
HHHHHHCCCCEEEEEECCCCEEEEEECCCCEEEEHHHHHHHCCCCCCCCCCCCCEEEHHH
LDELIEFTLHWMKGEKS
HHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKQRILMTFDKKDFSLLCFEKGTLYVDGEGGQLGDRGKIDGNKILQVKRVDGYPCAVIEK
CCCCEEEEECCCCCEEEEEECCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEC
KNNYSTGEFVELELDLERRVDIAQQHTAQHLLSAVALRELDAETVGFQMGEEFSTIDLTI
CCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCHHCCCCCCEEEEEE
GLLEDKMKRLLEDVSNDCILKLLPVTVKEVDPSEIFRYNLRKSVSDKILEKAEKIRLVKI
HHHHHHHHHHHHHCCCCEEEEEECCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEE
EGIDVSPCGGFHVKNTGEVGLLKILKVEKVKGNLTRVYFVAGKRAIRVFQNEHSILGSLG
ECEEECCCCCEEECCCCCCHHEEEEEHHHCCCCEEEEEEEECCCEEEHHHHHHHHHHHHH
KLLTCGYHELVSRTQSILEDLKIMNNRIKGYVERIAKETANNLSENSSELLLYEDDEEIL
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHH
SMIPRFLDKECYIFFGKAGEKIILASKGYDLTILVKELKSRFDIKGGAGKTRGQFVYSKE
HHHHHHCCCCEEEEEECCCCEEEEEECCCCEEEEHHHHHHHCCCCCCCCCCCCCEEEHHH
LDELIEFTLHWMKGEKS
HHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; @ALAN01.txt*L-alanine!; tRNAAla [C]

Specific reaction: ATP + @ALAN01.txt*L-alanine! + tRNAAla = AMP + diphosphate + L-alanyl-tRNAAla [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]