Click here to switch to the map view.

The map label for this gene is trmD [H]

Identifier: 239617838

GI number: 239617838

Start: 1563861

End: 1564607

Strand: Direct

Name: trmD [H]

Synonym: Kole_1464

Alternate gene names: 239617838

Gene position: 1563861-1564607 (Clockwise)

Preceding gene: 239617837

Following gene: 239617839

Centisome position: 67.93

GC content: 40.43

Gene sequence:

>747_bases
ATGAAAATCAGCGTATTGACGATATTTCCTAAAATGTTTTCGGTAATAACGGATTGGGGCGTTATATCCCGGGCAATAAA
AGAAGGGAAAATAGTTTTTGAAGCCATCGATCTCAGGGATTTCACTACTGATAAACACCGTACAACCGATGATTATCCTT
ACGGTGGTGGTAGTGGATTGGTGATGAAGGTCGATCCTTTTTTCAAAGCTTATGATCACATTGTGGAGGTTAATGAGGCG
AAACCCTATGTTGTTTTAACTTCTCCTCAGGGAAGGGTCTTTAACAACAACATTGCTAAAGAACTTTCTACCCATGATCA
CATCCTTTTTTTGTGTGGTAGATATGAAGGTATTGATGAAAGAATCACGACCATTGTGGATGATGAACTATCCATTGGAG
ATTTTGTCCTGAGTGGTGGTGAATTGGCTGCAATGGTAATGATAGAAGCAATTTCGCGATTTATTCCGGGTGTTGTGGGA
GACACAGATTCTGTTGTGAATGATTCCTTCTATAACGATTTGCTGGATTATCCGCATTACACACGCCCCAGAACAATCCG
TGGATTGGATGTACCGGAGGTTCTTCTTAGTGGAGATCATGAGAGGATAGAACTATACAGAAAGAAAGAAAGTCTCAGGA
GAACAATGGAAAGGCGTCCGGACCTGTTTTTGAAGCATGAATTTGACGAAATGGATAAAAAGGCACTCCTATTGTTGTTT
AAGGAGTTGATGTCTGGTGTTGAATAA

Upstream 100 bases:

>100_bases
GTGATCAAGGATTACATAGTAGAATTTCGTTTTAAGGATAAGAAGATTGTAGCGAAACGCCTTGAATACCTTACTCTGGA
AGGTAAAGAGGATAACGACG

Downstream 100 bases:

>100_bases
CATATACGTTGCGCTCGTACACTATCCCGTTTTGGGAAGAAATGGTAGGATAGTTTCCAGTGCAGTGACAAATCTGGACG
TTCATGATATAGCCAGAACC

Product: tRNA (guanine-N1)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MKISVLTIFPKMFSVITDWGVISRAIKEGKIVFEAIDLRDFTTDKHRTTDDYPYGGGSGLVMKVDPFFKAYDHIVEVNEA
KPYVVLTSPQGRVFNNNIAKELSTHDHILFLCGRYEGIDERITTIVDDELSIGDFVLSGGELAAMVMIEAISRFIPGVVG
DTDSVVNDSFYNDLLDYPHYTRPRTIRGLDVPEVLLSGDHERIELYRKKESLRRTMERRPDLFLKHEFDEMDKKALLLLF
KELMSGVE

Sequences:

>Translated_248_residues
MKISVLTIFPKMFSVITDWGVISRAIKEGKIVFEAIDLRDFTTDKHRTTDDYPYGGGSGLVMKVDPFFKAYDHIVEVNEA
KPYVVLTSPQGRVFNNNIAKELSTHDHILFLCGRYEGIDERITTIVDDELSIGDFVLSGGELAAMVMIEAISRFIPGVVG
DTDSVVNDSFYNDLLDYPHYTRPRTIRGLDVPEVLLSGDHERIELYRKKESLRRTMERRPDLFLKHEFDEMDKKALLLLF
KELMSGVE
>Mature_248_residues
MKISVLTIFPKMFSVITDWGVISRAIKEGKIVFEAIDLRDFTTDKHRTTDDYPYGGGSGLVMKVDPFFKAYDHIVEVNEA
KPYVVLTSPQGRVFNNNIAKELSTHDHILFLCGRYEGIDERITTIVDDELSIGDFVLSGGELAAMVMIEAISRFIPGVVG
DTDSVVNDSFYNDLLDYPHYTRPRTIRGLDVPEVLLSGDHERIELYRKKESLRRTMERRPDLFLKHEFDEMDKKALLLLF
KELMSGVE

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=242, Percent_Identity=48.7603305785124, Blast_Score=235, Evalue=3e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 28344; Mature: 28344

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISVLTIFPKMFSVITDWGVISRAIKEGKIVFEAIDLRDFTTDKHRTTDDYPYGGGSGL
CEEEEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHCCCCCCCCCCCCCCCCCCEE
VMKVDPFFKAYDHIVEVNEAKPYVVLTSPQGRVFNNNIAKELSTHDHILFLCGRYEGIDE
EEEECHHHHHHHHHEEECCCCCEEEEECCCCCEECCHHHHHHCCCCEEEEEECCCCCHHH
RITTIVDDELSIGDFVLSGGELAAMVMIEAISRFIPGVVGDTDSVVNDSFYNDLLDYPHY
HHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCC
TRPRTIRGLDVPEVLLSGDHERIELYRKKESLRRTMERRPDLFLKHEFDEMDKKALLLLF
CCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHH
KELMSGVE
HHHHHCCC
>Mature Secondary Structure
MKISVLTIFPKMFSVITDWGVISRAIKEGKIVFEAIDLRDFTTDKHRTTDDYPYGGGSGL
CEEEEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCHHCCCCCCCCCCCCCCCCCCEE
VMKVDPFFKAYDHIVEVNEAKPYVVLTSPQGRVFNNNIAKELSTHDHILFLCGRYEGIDE
EEEECHHHHHHHHHEEECCCCCEEEEECCCCCEECCHHHHHHCCCCEEEEEECCCCCHHH
RITTIVDDELSIGDFVLSGGELAAMVMIEAISRFIPGVVGDTDSVVNDSFYNDLLDYPHY
HHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCC
TRPRTIRGLDVPEVLLSGDHERIELYRKKESLRRTMERRPDLFLKHEFDEMDKKALLLLF
CCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHH
KELMSGVE
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA