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The map label for this gene is bhbA [H]

Identifier: 239617748

GI number: 239617748

Start: 1456116

End: 1457798

Strand: Reverse

Name: bhbA [H]

Synonym: Kole_1372

Alternate gene names: 239617748

Gene position: 1457798-1456116 (Counterclockwise)

Preceding gene: 239617752

Following gene: 239617747

Centisome position: 63.32

GC content: 45.34

Gene sequence:

>1683_bases
ATGTCCTTGAATCCTGAAGAGAAAAAGCACCTTGAAACCTTGAAAGAAAACTGGGAAAAGACAACCGTTGAAAAAGTTAT
TCAGAAATACCCGGAGAGAAAAGAACGATTCGAAACCTCCTTTGGTGAAGAGATTGAAAGGCTTTATACGCCGTTAGATA
TCGCTGAACTGGATTACGAACATGATCTTGGATTCCCCGGCGAATACCCCTTCACAAGAGGAGTACAACCAACCATGTAC
CGCGGTCGTTTCTGGACGATGAGACAGTACGCCGGTTTTGGAACTGCCGAAGAATCCAACAAACGTTACAAATACCTTCT
ATCACAGGGACAAACGGGGCTTTCAGTTGCCTTCGATCTCCCAACTCAGATAGGATACGACTCAGATGATCCCATGGCAG
AGGGCGAAATAGGAAAAGTCGGAGTTGCAATAGATTCACTGGAAGACATGGAGGTCCTTTTTGACGGAATTCCTCTGGAT
CAGGTAAGTACCTCCATGACGATAAACTCAACGGCTGCCATTTTACTCGCAATGTACATAGCAGTTGCCGAAAAACAGGG
CGTCTCCTCCGATAAACTGCGAGGAACGATACAAAACGACATCCTAAAAGAATACATCGCCAGAGGGACGTACATTTTCC
CGCCAGAACCTTCGATGAGAATAATCACAAACATCTTCGAATACTGTTCGAAACACCTACCCAAATTCAACACAATCAGT
ATCAGCGGTTACCACATACGTGAAGCCGGCGCAGATGCCGTCCAGGAGGTTGCTTTCACACTAGCAGATGGTATAGCCTA
CGTTGAAGCTGCCGTGAAAGCCGGCCTTGATCCCAATGTCTTCGGTAAGAGATTGTCCTTCTTCTTCAACGCTCACAACA
ACTTCCTGGAAGAAATAGCGAAGTTCAGAGCCGCGCGAAAACTCTGGAGTAAGATCATGCGGGAACGCTTTGGTGTAACC
GATAAAAAGGCCCTCATGTTGAGGTTCCATACTCAAACGGGAGGGTCAACGTTAACCGCTCAACAGCCGATGAACAATAT
TGTCAGGGTCGCTTTCCAGGCACTTGCCGCGGTTCTCGGAGGCACCCAGTCACTCCATACGAATTCTTACGACGAAGCTC
TGGGGCTTCCAACTGAAGAATCTGTTACGATAGCTCTTAGAACACAGCAGATAATAGCCCATGAAACAGGTGTCGCTGAT
GTTGTCGACCCGCTTGGCGGTTCATATTACATAGAGCATTTGACGAGCGAAATTGAAAAGAGAGCTACCGAATACATAAA
AAGGATAGATGAACTCGGTGGAATGGTTAGAGCGATCGAAGAAGGTTACATCCAAAAAGAAATATTGAATACAGCGTACA
AATATCAGCTAGAAATTGAAGAAGGATCGAAAGTGATCGTTGGAGTCAACAAATTTACCGGAGAAGAAACCACACCAAAG
AAGATTCTCAAAGTCGATGAAGAACTTGAAGAAAAACAGAAAGCAAGAGTACGTGCTCTCAGGGAAAAGCGTGATAACAT
AAAGGTGGAGAAAACCCTGAAAGAATTGAAAAACGCGGCCATGGGTAACGAAAACCTGATGCCTTATATTCTTAATGCCG
TAAAAGCCTACGCGACCATCGGTGAAATTTCGAATGTTTTGAGAGATGTCTTCGGCGAATACACGGAAGCTGTTATCCTC
TGA

Upstream 100 bases:

>100_bases
CATCTATCCGTTCCTATCTTTTCCCAATAGCTGTTGACTAAATAGCTTTTTTGATTTAGTTTTGAAGTGAGAAACTCCGA
AAAAAATTGGGGGGATGATG

Downstream 100 bases:

>100_bases
AACTTATTCAAATTGCGAGGTGGTAGAAATGGATAAGAAGATAAAAGTGTTAATAGCAAAACCGGGACTCGATGGACATG
ATAGAGGTGCGAAAGTTGTT

Product: methylmalonyl-CoA mutase, large subunit

Products: NA

Alternate protein names: MCM [H]

Number of amino acids: Translated: 560; Mature: 559

Protein sequence:

>560_residues
MSLNPEEKKHLETLKENWEKTTVEKVIQKYPERKERFETSFGEEIERLYTPLDIAELDYEHDLGFPGEYPFTRGVQPTMY
RGRFWTMRQYAGFGTAEESNKRYKYLLSQGQTGLSVAFDLPTQIGYDSDDPMAEGEIGKVGVAIDSLEDMEVLFDGIPLD
QVSTSMTINSTAAILLAMYIAVAEKQGVSSDKLRGTIQNDILKEYIARGTYIFPPEPSMRIITNIFEYCSKHLPKFNTIS
ISGYHIREAGADAVQEVAFTLADGIAYVEAAVKAGLDPNVFGKRLSFFFNAHNNFLEEIAKFRAARKLWSKIMRERFGVT
DKKALMLRFHTQTGGSTLTAQQPMNNIVRVAFQALAAVLGGTQSLHTNSYDEALGLPTEESVTIALRTQQIIAHETGVAD
VVDPLGGSYYIEHLTSEIEKRATEYIKRIDELGGMVRAIEEGYIQKEILNTAYKYQLEIEEGSKVIVGVNKFTGEETTPK
KILKVDEELEEKQKARVRALREKRDNIKVEKTLKELKNAAMGNENLMPYILNAVKAYATIGEISNVLRDVFGEYTEAVIL

Sequences:

>Translated_560_residues
MSLNPEEKKHLETLKENWEKTTVEKVIQKYPERKERFETSFGEEIERLYTPLDIAELDYEHDLGFPGEYPFTRGVQPTMY
RGRFWTMRQYAGFGTAEESNKRYKYLLSQGQTGLSVAFDLPTQIGYDSDDPMAEGEIGKVGVAIDSLEDMEVLFDGIPLD
QVSTSMTINSTAAILLAMYIAVAEKQGVSSDKLRGTIQNDILKEYIARGTYIFPPEPSMRIITNIFEYCSKHLPKFNTIS
ISGYHIREAGADAVQEVAFTLADGIAYVEAAVKAGLDPNVFGKRLSFFFNAHNNFLEEIAKFRAARKLWSKIMRERFGVT
DKKALMLRFHTQTGGSTLTAQQPMNNIVRVAFQALAAVLGGTQSLHTNSYDEALGLPTEESVTIALRTQQIIAHETGVAD
VVDPLGGSYYIEHLTSEIEKRATEYIKRIDELGGMVRAIEEGYIQKEILNTAYKYQLEIEEGSKVIVGVNKFTGEETTPK
KILKVDEELEEKQKARVRALREKRDNIKVEKTLKELKNAAMGNENLMPYILNAVKAYATIGEISNVLRDVFGEYTEAVIL
>Mature_559_residues
SLNPEEKKHLETLKENWEKTTVEKVIQKYPERKERFETSFGEEIERLYTPLDIAELDYEHDLGFPGEYPFTRGVQPTMYR
GRFWTMRQYAGFGTAEESNKRYKYLLSQGQTGLSVAFDLPTQIGYDSDDPMAEGEIGKVGVAIDSLEDMEVLFDGIPLDQ
VSTSMTINSTAAILLAMYIAVAEKQGVSSDKLRGTIQNDILKEYIARGTYIFPPEPSMRIITNIFEYCSKHLPKFNTISI
SGYHIREAGADAVQEVAFTLADGIAYVEAAVKAGLDPNVFGKRLSFFFNAHNNFLEEIAKFRAARKLWSKIMRERFGVTD
KKALMLRFHTQTGGSTLTAQQPMNNIVRVAFQALAAVLGGTQSLHTNSYDEALGLPTEESVTIALRTQQIIAHETGVADV
VDPLGGSYYIEHLTSEIEKRATEYIKRIDELGGMVRAIEEGYIQKEILNTAYKYQLEIEEGSKVIVGVNKFTGEETTPKK
ILKVDEELEEKQKARVRALREKRDNIKVEKTLKELKNAAMGNENLMPYILNAVKAYATIGEISNVLRDVFGEYTEAVIL

Specific function: Catalyzes the interconversion of succinyl-CoA and methylmalonyl-CoA during the polyhydroxyalkanoate degradation pathway [H]

COG id: COG1884

COG function: function code I; Methylmalonyl-CoA mutase, N-terminal domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156105689, Length=545, Percent_Identity=49.3577981651376, Blast_Score=526, Evalue=1e-149,
Organism=Escherichia coli, GI1789284, Length=518, Percent_Identity=52.1235521235521, Blast_Score=513, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI25144500, Length=519, Percent_Identity=52.4084778420039, Blast_Score=543, Evalue=1e-155,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006159
- InterPro:   IPR016176
- InterPro:   IPR014348
- InterPro:   IPR006158
- InterPro:   IPR006099
- InterPro:   IPR006098 [H]

Pfam domain/function: PF02310 B12-binding; PF01642 MM_CoA_mutase [H]

EC number: =5.4.99.2 [H]

Molecular weight: Translated: 63087; Mature: 62955

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLNPEEKKHLETLKENWEKTTVEKVIQKYPERKERFETSFGEEIERLYTPLDIAELDYE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCC
HDLGFPGEYPFTRGVQPTMYRGRFWTMRQYAGFGTAEESNKRYKYLLSQGQTGLSVAFDL
CCCCCCCCCCCCCCCCCCCCCCCEEEHHHHCCCCCCCCCCHHHHHHHHCCCCCCEEEEEC
PTQIGYDSDDPMAEGEIGKVGVAIDSLEDMEVLFDGIPLDQVSTSMTINSTAAILLAMYI
CHHCCCCCCCCCCCCCCCEEEEEHHCHHHHHHHHCCCCHHHHCCCEEEHHHHHHHHHHHH
AVAEKQGVSSDKLRGTIQNDILKEYIARGTYIFPPEPSMRIITNIFEYCSKHLPKFNTIS
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHCCCCCEEE
ISGYHIREAGADAVQEVAFTLADGIAYVEAAVKAGLDPNVFGKRLSFFFNAHNNFLEEIA
ECCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
KFRAARKLWSKIMRERFGVTDKKALMLRFHTQTGGSTLTAQQPMNNIVRVAFQALAAVLG
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHC
GTQSLHTNSYDEALGLPTEESVTIALRTQQIIAHETGVADVVDPLGGSYYIEHLTSEIEK
CCCCCCCCCCHHHCCCCCCCCEEEEEEHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHH
RATEYIKRIDELGGMVRAIEEGYIQKEILNTAYKYQLEIEEGSKVIVGVNKFTGEETTPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCEEEEEEHHCCCCCCCHH
KILKVDEELEEKQKARVRALREKRDNIKVEKTLKELKNAAMGNENLMPYILNAVKAYATI
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
GEISNVLRDVFGEYTEAVIL
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLNPEEKKHLETLKENWEKTTVEKVIQKYPERKERFETSFGEEIERLYTPLDIAELDYE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCC
HDLGFPGEYPFTRGVQPTMYRGRFWTMRQYAGFGTAEESNKRYKYLLSQGQTGLSVAFDL
CCCCCCCCCCCCCCCCCCCCCCCEEEHHHHCCCCCCCCCCHHHHHHHHCCCCCCEEEEEC
PTQIGYDSDDPMAEGEIGKVGVAIDSLEDMEVLFDGIPLDQVSTSMTINSTAAILLAMYI
CHHCCCCCCCCCCCCCCCEEEEEHHCHHHHHHHHCCCCHHHHCCCEEEHHHHHHHHHHHH
AVAEKQGVSSDKLRGTIQNDILKEYIARGTYIFPPEPSMRIITNIFEYCSKHLPKFNTIS
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHCCCCCEEE
ISGYHIREAGADAVQEVAFTLADGIAYVEAAVKAGLDPNVFGKRLSFFFNAHNNFLEEIA
ECCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
KFRAARKLWSKIMRERFGVTDKKALMLRFHTQTGGSTLTAQQPMNNIVRVAFQALAAVLG
HHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHC
GTQSLHTNSYDEALGLPTEESVTIALRTQQIIAHETGVADVVDPLGGSYYIEHLTSEIEK
CCCCCCCCCCHHHCCCCCCCCEEEEEEHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHH
RATEYIKRIDELGGMVRAIEEGYIQKEILNTAYKYQLEIEEGSKVIVGVNKFTGEETTPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCEEEEEEHHCCCCCCCHH
KILKVDEELEEKQKARVRALREKRDNIKVEKTLKELKNAAMGNENLMPYILNAVKAYATI
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
GEISNVLRDVFGEYTEAVIL
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9889346; 11481431 [H]