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The map label for this gene is meaA [H]

Identifier: 239617747

GI number: 239617747

Start: 1455692

End: 1456087

Strand: Reverse

Name: meaA [H]

Synonym: Kole_1371

Alternate gene names: 239617747

Gene position: 1456087-1455692 (Counterclockwise)

Preceding gene: 239617748

Following gene: 239617746

Centisome position: 63.25

GC content: 41.92

Gene sequence:

>396_bases
ATGGATAAGAAGATAAAAGTGTTAATAGCAAAACCGGGACTCGATGGACATGATAGAGGTGCGAAAGTTGTTGCTATGGC
TTTGAGAGATGCCGGAATGGAGGTTATTTATACAGGCCTCAGGCAATCACCAGAGAAAATCGTCAAAGACGCGTTGCAGG
AGGATGTTGATATCATAGGATTATCTATACTTTCAGGAGCCCATATGCGGCTTTGCGAAAAAGTCCTGAAATTGATGAAA
GAAGCCGGGATAGAATCCAAACCAATCTTCGTCGGTGGAATAATACCGGAAGATGATGCAAAAGAACTCAAAAAAATGGG
CATATACGAGGTCTTTGGTCCCGGTACCCCATTAGCAAATATCATAGAAAGAATTAAGGCTGCGGTGAAAAAATGA

Upstream 100 bases:

>100_bases
GCGACCATCGGTGAAATTTCGAATGTTTTGAGAGATGTCTTCGGCGAATACACGGAAGCTGTTATCCTCTGAAACTTATT
CAAATTGCGAGGTGGTAGAA

Downstream 100 bases:

>100_bases
AGATAGAGAAATTGCTGTCGAAAGACCCACTCTGGATCTCTAAAGCACTTACCACCGTCGAAAGGGATCCAATAGCTGGA
AAGGAACTGATAGATTCTCT

Product: cobalamin B12-binding domain protein

Products: succinyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 131; Mature: 131

Protein sequence:

>131_residues
MDKKIKVLIAKPGLDGHDRGAKVVAMALRDAGMEVIYTGLRQSPEKIVKDALQEDVDIIGLSILSGAHMRLCEKVLKLMK
EAGIESKPIFVGGIIPEDDAKELKKMGIYEVFGPGTPLANIIERIKAAVKK

Sequences:

>Translated_131_residues
MDKKIKVLIAKPGLDGHDRGAKVVAMALRDAGMEVIYTGLRQSPEKIVKDALQEDVDIIGLSILSGAHMRLCEKVLKLMK
EAGIESKPIFVGGIIPEDDAKELKKMGIYEVFGPGTPLANIIERIKAAVKK
>Mature_131_residues
MDKKIKVLIAKPGLDGHDRGAKVVAMALRDAGMEVIYTGLRQSPEKIVKDALQEDVDIIGLSILSGAHMRLCEKVLKLMK
EAGIESKPIFVGGIIPEDDAKELKKMGIYEVFGPGTPLANIIERIKAAVKK

Specific function: Required for the assimilation of methanol and ethanol [H]

COG id: COG2185

COG function: function code I; Methylmalonyl-CoA mutase, C-terminal domain/subunit (cobalamin-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156105689, Length=114, Percent_Identity=37.719298245614, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1789284, Length=123, Percent_Identity=36.5853658536585, Blast_Score=96, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI25144500, Length=129, Percent_Identity=39.5348837209302, Blast_Score=89, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006159
- InterPro:   IPR016176
- InterPro:   IPR014348
- InterPro:   IPR006158
- InterPro:   IPR006099
- InterPro:   IPR006098 [H]

Pfam domain/function: PF02310 B12-binding; PF01642 MM_CoA_mutase [H]

EC number: 5.4.99.2

Molecular weight: Translated: 14235; Mature: 14235

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKKIKVLIAKPGLDGHDRGAKVVAMALRDAGMEVIYTGLRQSPEKIVKDALQEDVDIIG
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LSILSGAHMRLCEKVLKLMKEAGIESKPIFVGGIIPEDDAKELKKMGIYEVFGPGTPLAN
HHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHCCCHHCCCCCCCHHH
IIERIKAAVKK
HHHHHHHHHCC
>Mature Secondary Structure
MDKKIKVLIAKPGLDGHDRGAKVVAMALRDAGMEVIYTGLRQSPEKIVKDALQEDVDIIG
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LSILSGAHMRLCEKVLKLMKEAGIESKPIFVGGIIPEDDAKELKKMGIYEVFGPGTPLAN
HHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHCCCHHCCCCCCCHHH
IIERIKAAVKK
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-methylmalonyl-CoA

Specific reaction: (R)-methylmalonyl-CoA = succinyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8868443 [H]