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The map label for this gene is ywfA [H]

Identifier: 239617592

GI number: 239617592

Start: 1288696

End: 1289991

Strand: Reverse

Name: ywfA [H]

Synonym: Kole_1212

Alternate gene names: 239617592

Gene position: 1289991-1288696 (Counterclockwise)

Preceding gene: 239617593

Following gene: 239617591

Centisome position: 56.03

GC content: 43.29

Gene sequence:

>1296_bases
ATGAACAAGTGGTGGAGTGTTATTTTTCTATCTATTCTTCTTATTTTTCTCAATGCTGATCAAATGGTTATGGCACCAAA
TATTGGTGCCATAGAAGAGGAATTCGACGTCAACGACGCCCTAATCGGGCTTGTCGCTTCTACTTTTACCGTTCTCGGAG
CTGTTATCAGTTTGGTCTGGGGATTTCTAGCAGATAAATACAACAGAAAGAAGATTTTTATATACTCCATTCTTATCGGT
GAGATTCCATGTGTTATGTCCGCGTTTTCCGGGAGCTTCGGCGAGCTCTTTTTGTGGCGTACGCTTACCGGTATAGGGGT
GGGTGCATCTTTTCCAATTGCGTTTTCCTTTGTTAGCGATATGTTTGGTAAAGATGAAAGAGGAAAGGTTGCTGCCGTAC
TTGGACTTTCAATTTCCATCGGTAATATCCTGGGAATGATCTTCGGAGGTTATTTTGGTGCTGCATATGGTTGGCGGATA
CCCTTTCTTATGGTCTCGCTTCCCAACATCGTACTAGCAGGCCTTGGATTCTTTATTCTCAAGGAACCACCACGGGGCGC
GATGGAAAAAGGAATCGGGGATCTCGTGCAAATGGGGTATGCCTATCCAAAAGGTGTCAAACTTTCAGATTATTTAGATC
TCTTCAGGATTAAAACAAACCTCTTGCTCTTCTTCCAGGGGATCGCCGGAACGATACCATGGGGTGCTATTCCGTATTTT
ATGGTGGAGTATTTCAAAAGGGAAATGGGGTTTTCCGCCTCGCAAGCCACTACCATTTTTCTTATATTCGGTGCAGGAAA
TATCGTTGGAATGTTAATCGGAGGCTGGTTGGGGCAAAAACTGTACAATAAATCACCAAGGCTTGTTCCTGTAGCATCAG
GGATTACAACAATTTTAGGTGCAATTTTGACGATTTTCGTTTTCTCGGAAAACATTATAGCTGGATCATTCATACTCGTT
TCGTCCATTGGTTTTGTTGCTGCCCTCTTCGATAGCTACACTGGTCCAAACGTAAAGATGATGCTCATGAATGTGAATGA
ACCACAGAGTAGAGGGAGGATTTTCTCCATATTCAATCTCACGGATTCTCTGGGAACCGGTATTGGTAAGCTCGTGGGTG
GTACGCTTTCTGTCTCTCTGGGAAGTCTAGCAATAGCCATGAAGGTTTCCAGCGCGTTCTGGTTCATCTGTGGTGCGATT
ATATTGGTACTCGCTTTCTTTTTTGAAAAGGATATTTCCTCTCTCGACGAGAAGATGGAAAAACTGAAAGAGCAAATGAA
AAGCAGAAAAGCTTGA

Upstream 100 bases:

>100_bases
CGACGTAAAACGTCGCCCAGCATCAACAACTTCCGTCATTTTTGTTGTAGCTATATAGTTAATGTTAAAATCAAATTAAT
AGCAGTAAGGGGGGAAGCAG

Downstream 100 bases:

>100_bases
AATAGGTGCCGTTTTGACAAGGAAAATTGGGCACTTCGTTCGTAGCTTGTTAAAAGCACCAGGGGGAATGGAAATTGAGA
GTGGTCTCATGTGTGGTTGA

Product: major facilitator superfamily MFS_1

Products: Proton [Cytoplasm]; 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MNKWWSVIFLSILLIFLNADQMVMAPNIGAIEEEFDVNDALIGLVASTFTVLGAVISLVWGFLADKYNRKKIFIYSILIG
EIPCVMSAFSGSFGELFLWRTLTGIGVGASFPIAFSFVSDMFGKDERGKVAAVLGLSISIGNILGMIFGGYFGAAYGWRI
PFLMVSLPNIVLAGLGFFILKEPPRGAMEKGIGDLVQMGYAYPKGVKLSDYLDLFRIKTNLLLFFQGIAGTIPWGAIPYF
MVEYFKREMGFSASQATTIFLIFGAGNIVGMLIGGWLGQKLYNKSPRLVPVASGITTILGAILTIFVFSENIIAGSFILV
SSIGFVAALFDSYTGPNVKMMLMNVNEPQSRGRIFSIFNLTDSLGTGIGKLVGGTLSVSLGSLAIAMKVSSAFWFICGAI
ILVLAFFFEKDISSLDEKMEKLKEQMKSRKA

Sequences:

>Translated_431_residues
MNKWWSVIFLSILLIFLNADQMVMAPNIGAIEEEFDVNDALIGLVASTFTVLGAVISLVWGFLADKYNRKKIFIYSILIG
EIPCVMSAFSGSFGELFLWRTLTGIGVGASFPIAFSFVSDMFGKDERGKVAAVLGLSISIGNILGMIFGGYFGAAYGWRI
PFLMVSLPNIVLAGLGFFILKEPPRGAMEKGIGDLVQMGYAYPKGVKLSDYLDLFRIKTNLLLFFQGIAGTIPWGAIPYF
MVEYFKREMGFSASQATTIFLIFGAGNIVGMLIGGWLGQKLYNKSPRLVPVASGITTILGAILTIFVFSENIIAGSFILV
SSIGFVAALFDSYTGPNVKMMLMNVNEPQSRGRIFSIFNLTDSLGTGIGKLVGGTLSVSLGSLAIAMKVSSAFWFICGAI
ILVLAFFFEKDISSLDEKMEKLKEQMKSRKA
>Mature_431_residues
MNKWWSVIFLSILLIFLNADQMVMAPNIGAIEEEFDVNDALIGLVASTFTVLGAVISLVWGFLADKYNRKKIFIYSILIG
EIPCVMSAFSGSFGELFLWRTLTGIGVGASFPIAFSFVSDMFGKDERGKVAAVLGLSISIGNILGMIFGGYFGAAYGWRI
PFLMVSLPNIVLAGLGFFILKEPPRGAMEKGIGDLVQMGYAYPKGVKLSDYLDLFRIKTNLLLFFQGIAGTIPWGAIPYF
MVEYFKREMGFSASQATTIFLIFGAGNIVGMLIGGWLGQKLYNKSPRLVPVASGITTILGAILTIFVFSENIIAGSFILV
SSIGFVAALFDSYTGPNVKMMLMNVNEPQSRGRIFSIFNLTDSLGTGIGKLVGGTLSVSLGSLAIAMKVSSAFWFICGAI
ILVLAFFFEKDISSLDEKMEKLKEQMKSRKA

Specific function: Could Be A Transporter For 3-Phenylpropionate (Hydrocinnamic Acid). [C]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Homo sapiens, GI215490100, Length=174, Percent_Identity=29.8850574712644, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI216548223, Length=176, Percent_Identity=27.8409090909091, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI215490102, Length=174, Percent_Identity=29.8850574712644, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI215490096, Length=174, Percent_Identity=29.8850574712644, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI14042968, Length=174, Percent_Identity=29.8850574712644, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI87081723, Length=276, Percent_Identity=28.2608695652174, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193209775, Length=467, Percent_Identity=25.2676659528908, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17557872, Length=439, Percent_Identity=24.8291571753986, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17539092, Length=447, Percent_Identity=23.9373601789709, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71992409, Length=287, Percent_Identity=27.5261324041812, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI17864456, Length=295, Percent_Identity=24.7457627118644, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24654039, Length=336, Percent_Identity=24.4047619047619, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24654041, Length=294, Percent_Identity=24.4897959183673, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24654037, Length=294, Percent_Identity=24.4897959183673, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24654044, Length=189, Percent_Identity=26.984126984127, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46877; Mature: 46877

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKWWSVIFLSILLIFLNADQMVMAPNIGAIEEEFDVNDALIGLVASTFTVLGAVISLVW
CCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHH
GFLADKYNRKKIFIYSILIGEIPCVMSAFSGSFGELFLWRTLTGIGVGASFPIAFSFVSD
HHHHCCCCCCEEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHH
MFGKDERGKVAAVLGLSISIGNILGMIFGGYFGAAYGWRIPFLMVSLPNIVLAGLGFFIL
HHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
KEPPRGAMEKGIGDLVQMGYAYPKGVKLSDYLDLFRIKTNLLLFFQGIAGTIPWGAIPYF
CCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
MVEYFKREMGFSASQATTIFLIFGAGNIVGMLIGGWLGQKLYNKSPRLVPVASGITTILG
HHHHHHHHHCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHH
AILTIFVFSENIIAGSFILVSSIGFVAALFDSYTGPNVKMMLMNVNEPQSRGRIFSIFNL
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEEEEEC
TDSLGTGIGKLVGGTLSVSLGSLAIAMKVSSAFWFICGAIILVLAFFFEKDISSLDEKME
HHHHCCCHHHHHCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLKEQMKSRKA
HHHHHHHHCCC
>Mature Secondary Structure
MNKWWSVIFLSILLIFLNADQMVMAPNIGAIEEEFDVNDALIGLVASTFTVLGAVISLVW
CCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHH
GFLADKYNRKKIFIYSILIGEIPCVMSAFSGSFGELFLWRTLTGIGVGASFPIAFSFVSD
HHHHCCCCCCEEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHH
MFGKDERGKVAAVLGLSISIGNILGMIFGGYFGAAYGWRIPFLMVSLPNIVLAGLGFFIL
HHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
KEPPRGAMEKGIGDLVQMGYAYPKGVKLSDYLDLFRIKTNLLLFFQGIAGTIPWGAIPYF
CCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
MVEYFKREMGFSASQATTIFLIFGAGNIVGMLIGGWLGQKLYNKSPRLVPVASGITTILG
HHHHHHHHHCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHH
AILTIFVFSENIIAGSFILVSSIGFVAALFDSYTGPNVKMMLMNVNEPQSRGRIFSIFNL
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEEEEEC
TDSLGTGIGKLVGGTLSVSLGSLAIAMKVSSAFWFICGAIILVLAFFFEKDISSLDEKME
HHHHCCCHHHHHCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLKEQMKSRKA
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 3-(3-hydroxyphenyl)propionate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 3-(3-hydroxyphenyl)propionate [Periplasm] = Proton [Cytoplasm] + 3-(3-hydroxyphenyl)propionate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]