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The map label for this gene is nagJ [H]

Identifier: 239617557

GI number: 239617557

Start: 1244447

End: 1245838

Strand: Reverse

Name: nagJ [H]

Synonym: Kole_1175

Alternate gene names: 239617557

Gene position: 1245838-1244447 (Counterclockwise)

Preceding gene: 239617558

Following gene: 239617551

Centisome position: 54.12

GC content: 42.31

Gene sequence:

>1392_bases
ATGAATGCTTTCAAAATTCGCGGTGTTGTCGAAGGGTTCTATGGAAAGCCCTGGTCTATGGAAGACAGAATGGAAATGAT
TCCTTTCCTGGGGAATCATGGTTATAACCTCTATATTTACGCTCCAAAGGATGATTCGTTTCACAGGTACAGATGGCGTG
AAACTTATTCATATTCTTTTATGAAAGATTTTGAGAAGCTTGTCAAAACAGGTGAGAAAAGTGGTGTTGAGGTCGCTTTT
GCCATCAGCCCTGGCCTTTCAATTGTCCACAGTGATCCCGAAGAATTGAGGACCATCGTTGAAAAGTACCTGAGCTTCGC
AAAACTCGGCGTCAGGAGTTTTTGCCTTTTTTATGACGACATCCCGCCAAGTCTCTCACCTGAAGATGCTGAAAAATTTG
GCAATCTAGCCGAAGCCCAAATATATTTCGCAAATTCGGTCTATGAGGAACTGAAAAAGAATCTTGAAAATACTCTTTTC
ATAGTCTGTCCAACCGAATATTGCACAAACTACGATACTCCATACCTACGAAAATACGGTGAAAAACTCAACCCGGAAAT
ACACATATTCTGGACCGGACCCGAATGTTGTTCGAAGGACATCCCCGAAAGTGACGCAATAATGATATCTAAAACCATCA
AACGCAAACCTCTGTACTGGGACAACTATCCGGTAAATGATTCCTACATGGTCCCGGAACTCCATATCGGTCCTTATACC
GGGAGAGCCCCTGAACTCGTGAATTATTCAGCCGGACTTCTCCTGAATCCAATGAACCTGCCGGAAGCTTCTAAGGTAGT
TCTCGCTGCGGCCTCTAGATTTTTACTGGATCCATACAATTACAATGCTGAGAAAGCGTGGGAAGAAGCTCTAGAAGAAA
TCTTCGGTGAGGCTCACGAAGAAGCGAAGCACTTCGCTCTCTGCAATCTAACCAGTCCTTTATATCCTGAAGAGCCTGAA
ATAACGAAAGAAATCGTTGAAAAATTCCATGGTCTATTCAAAATGGGAAAATTTGATGAGGCAATAGAACACTTAAAAAA
CGAAGCTGAAAAATTCATAAAGAATGCAGAGAAACTCAGAGAAAATATTTCCAGGAAGATGTTGCGGGATATAGAGCCCT
GGCTGAAAGAATATGAAGCATGGGGAAAAATACTTGGATTTATCGTAAAAGTGCTCGAGGTTCGAAAGGTACTTTACACT
GAAAAGTTCAAAAAAGAAGATCTTTACAAGATTAAAGAAGCTATTCTCGCTCTCCAAAAGCATCTATCTGAACTCACGGG
GCAGAATACCCTGTGCTGCGGCAGCGCTCTACGGGATTTCGCCATGGAAGTGCTTGTGAGAACAAAAGGAATCCTGAAGC
TCTTTGATTACACGACACCTTCAAGTTCTTGA

Upstream 100 bases:

>100_bases
CAAACTTGCAAAAACTGGCAACGATGCGGGTATAATAGGTGCAGCACTCTACAGTATGTATAAAAACGCAAAAATTTGAA
TGTGAGGGGAAAGATAGGAA

Downstream 100 bases:

>100_bases
TATATCGCCAACCTCCCTAGTCAGTCTAGAAAAAACGCCTCCCTCTGCGGGAGGCGTTTTGGTTTCAACAGTACTATGGA
CCTAAAACTTCCCCGATTAT

Product: Hyaluronidase

Products: NA

Alternate protein names: Beta-N-acetylhexosaminidase; Beta-hexosaminidase; GH84; Hexosaminidase B; N-acetyl-beta-glucosaminidase [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MNAFKIRGVVEGFYGKPWSMEDRMEMIPFLGNHGYNLYIYAPKDDSFHRYRWRETYSYSFMKDFEKLVKTGEKSGVEVAF
AISPGLSIVHSDPEELRTIVEKYLSFAKLGVRSFCLFYDDIPPSLSPEDAEKFGNLAEAQIYFANSVYEELKKNLENTLF
IVCPTEYCTNYDTPYLRKYGEKLNPEIHIFWTGPECCSKDIPESDAIMISKTIKRKPLYWDNYPVNDSYMVPELHIGPYT
GRAPELVNYSAGLLLNPMNLPEASKVVLAAASRFLLDPYNYNAEKAWEEALEEIFGEAHEEAKHFALCNLTSPLYPEEPE
ITKEIVEKFHGLFKMGKFDEAIEHLKNEAEKFIKNAEKLRENISRKMLRDIEPWLKEYEAWGKILGFIVKVLEVRKVLYT
EKFKKEDLYKIKEAILALQKHLSELTGQNTLCCGSALRDFAMEVLVRTKGILKLFDYTTPSSS

Sequences:

>Translated_463_residues
MNAFKIRGVVEGFYGKPWSMEDRMEMIPFLGNHGYNLYIYAPKDDSFHRYRWRETYSYSFMKDFEKLVKTGEKSGVEVAF
AISPGLSIVHSDPEELRTIVEKYLSFAKLGVRSFCLFYDDIPPSLSPEDAEKFGNLAEAQIYFANSVYEELKKNLENTLF
IVCPTEYCTNYDTPYLRKYGEKLNPEIHIFWTGPECCSKDIPESDAIMISKTIKRKPLYWDNYPVNDSYMVPELHIGPYT
GRAPELVNYSAGLLLNPMNLPEASKVVLAAASRFLLDPYNYNAEKAWEEALEEIFGEAHEEAKHFALCNLTSPLYPEEPE
ITKEIVEKFHGLFKMGKFDEAIEHLKNEAEKFIKNAEKLRENISRKMLRDIEPWLKEYEAWGKILGFIVKVLEVRKVLYT
EKFKKEDLYKIKEAILALQKHLSELTGQNTLCCGSALRDFAMEVLVRTKGILKLFDYTTPSSS
>Mature_463_residues
MNAFKIRGVVEGFYGKPWSMEDRMEMIPFLGNHGYNLYIYAPKDDSFHRYRWRETYSYSFMKDFEKLVKTGEKSGVEVAF
AISPGLSIVHSDPEELRTIVEKYLSFAKLGVRSFCLFYDDIPPSLSPEDAEKFGNLAEAQIYFANSVYEELKKNLENTLF
IVCPTEYCTNYDTPYLRKYGEKLNPEIHIFWTGPECCSKDIPESDAIMISKTIKRKPLYWDNYPVNDSYMVPELHIGPYT
GRAPELVNYSAGLLLNPMNLPEASKVVLAAASRFLLDPYNYNAEKAWEEALEEIFGEAHEEAKHFALCNLTSPLYPEEPE
ITKEIVEKFHGLFKMGKFDEAIEHLKNEAEKFIKNAEKLRENISRKMLRDIEPWLKEYEAWGKILGFIVKVLEVRKVLYT
EKFKKEDLYKIKEAILALQKHLSELTGQNTLCCGSALRDFAMEVLVRTKGILKLFDYTTPSSS

Specific function: Biological function unknown. Capable of hydrolyzing the glycosidic link of O-GlcNAcylated proteins [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 fibronectin type-III domain [H]

Homologues:

Organism=Homo sapiens, GI11024698, Length=267, Percent_Identity=42.6966292134831, Blast_Score=211, Evalue=8e-55,
Organism=Homo sapiens, GI215490056, Length=267, Percent_Identity=42.6966292134831, Blast_Score=211, Evalue=8e-55,
Organism=Caenorhabditis elegans, GI193211004, Length=271, Percent_Identity=37.2693726937269, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI193211002, Length=271, Percent_Identity=37.2693726937269, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24648670, Length=261, Percent_Identity=37.1647509578544, Blast_Score=186, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015882
- InterPro:   IPR011496
- InterPro:   IPR008965
- InterPro:   IPR000421
- InterPro:   IPR018452
- InterPro:   IPR008957
- InterPro:   IPR003961
- InterPro:   IPR008979
- InterPro:   IPR017853
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00754 F5_F8_type_C; PF00041 fn3; PF02838 Glyco_hydro_20b; PF07555 NAGidase [H]

EC number: =3.2.1.52 [H]

Molecular weight: Translated: 53575; Mature: 53575

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAFKIRGVVEGFYGKPWSMEDRMEMIPFLGNHGYNLYIYAPKDDSFHRYRWRETYSYSF
CCCEEEHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHH
MKDFEKLVKTGEKSGVEVAFAISPGLSIVHSDPEELRTIVEKYLSFAKLGVRSFCLFYDD
HHHHHHHHHCCCCCCCEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
IPPSLSPEDAEKFGNLAEAQIYFANSVYEELKKNLENTLFIVCPTEYCTNYDTPYLRKYG
CCCCCCHHHHHHHCCHHHHHEEHHHHHHHHHHHHHCCEEEEEECHHHHCCCCCHHHHHHH
EKLNPEIHIFWTGPECCSKDIPESDAIMISKTIKRKPLYWDNYPVNDSYMVPELHIGPYT
HHCCCCEEEEECCHHHHCCCCCCCCCEEHHHHHHCCCCCCCCCCCCCCEECCEEEECCCC
GRAPELVNYSAGLLLNPMNLPEASKVVLAAASRFLLDPYNYNAEKAWEEALEEIFGEAHE
CCCCCHHCCCCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
EAKHFALCNLTSPLYPEEPEITKEIVEKFHGLFKMGKFDEAIEHLKNEAEKFIKNAEKLR
HHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
ENISRKMLRDIEPWLKEYEAWGKILGFIVKVLEVRKVLYTEKFKKEDLYKIKEAILALQK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HLSELTGQNTLCCGSALRDFAMEVLVRTKGILKLFDYTTPSSS
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNAFKIRGVVEGFYGKPWSMEDRMEMIPFLGNHGYNLYIYAPKDDSFHRYRWRETYSYSF
CCCEEEHHHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHH
MKDFEKLVKTGEKSGVEVAFAISPGLSIVHSDPEELRTIVEKYLSFAKLGVRSFCLFYDD
HHHHHHHHHCCCCCCCEEEEEECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
IPPSLSPEDAEKFGNLAEAQIYFANSVYEELKKNLENTLFIVCPTEYCTNYDTPYLRKYG
CCCCCCHHHHHHHCCHHHHHEEHHHHHHHHHHHHHCCEEEEEECHHHHCCCCCHHHHHHH
EKLNPEIHIFWTGPECCSKDIPESDAIMISKTIKRKPLYWDNYPVNDSYMVPELHIGPYT
HHCCCCEEEEECCHHHHCCCCCCCCCEEHHHHHHCCCCCCCCCCCCCCEECCEEEECCCC
GRAPELVNYSAGLLLNPMNLPEASKVVLAAASRFLLDPYNYNAEKAWEEALEEIFGEAHE
CCCCCHHCCCCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
EAKHFALCNLTSPLYPEEPEITKEIVEKFHGLFKMGKFDEAIEHLKNEAEKFIKNAEKLR
HHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
ENISRKMLRDIEPWLKEYEAWGKILGFIVKVLEVRKVLYTEKFKKEDLYKIKEAILALQK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HLSELTGQNTLCCGSALRDFAMEVLVRTKGILKLFDYTTPSSS
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA