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The map label for this gene is yfkN [H]

Identifier: 239617497

GI number: 239617497

Start: 1181079

End: 1182872

Strand: Reverse

Name: yfkN [H]

Synonym: Kole_1113

Alternate gene names: 239617497

Gene position: 1182872-1181079 (Counterclockwise)

Preceding gene: 239617501

Following gene: 239617496

Centisome position: 51.38

GC content: 44.65

Gene sequence:

>1794_bases
ATGAGGAAAACCTTTTTGGTATTTTTAATACTCCTGCTCGCTTTGTCGATGCTGGCCAAAACCGTCACTCTAACAATCTT
GCACACCAGTGACTTGCACGGAAACATCTTTCCCATCAACTACGCTACCAATAAACCTTACTACGTCGGGCTTGGAAAGA
TTCAAAGCCTCGTGAGAGCAGTTAAAGCTGAAAATCCAAACATTCTTCTCATCGACACAGGCGATCTGATTCAGGGAACT
CCTTTAGAGTATTACCACGCTCGTATCGATAACGAACCGGTAGACCCTATGGTTCTTGTTATGAATTACATGGGGTATCA
ATGCAGCATACTCGGGAACCACGAATTCAACTACGGCATGAATATTCTAAACAAGGCTGTTTCAGAAGCTAACTTCCCAT
TCCTGAGTGCCAATATAGTCAAAAAAGGGACCGAAGAACCTTATTTCAAGCCGTATCTGATCACTGAAGTTGACGGTGTA
AAGGTCGGGATTCTGGGATTGACTACAAAGTTTATTCCTAACTGGGAAGATCCGAAGAATATCGCTGAGCTGGATTTCCT
TGATCCTGTGGAAGTAGCGAAAAAATACGTTAAAATCCTCAGGGAAGAGGAAGGCGTCGATGTGGTGATAGTTGGCTATC
ATGGTGGACTGGAACGCGATCCAAAAACGGGCGAACCAACAGAGGAACTAACTGGCGAAAATCAGGGCTACCAGTTGATC
GAAGAAGTGCCCGGAATCGATGCTCTTCTCACCGGACATCAGCACAGATCAATAGCTGAAATCATCAACGGTGTGGCTGT
TACCCAGCCAAGAAACTGGGGGAAAGCCCTCGGTAAGATTGACATCGTTCTCGACGATTCCAACGGAAAATGGGAAGTAG
TGGATAAAAAGGTAGAACTTCTCAGCTCAAAGACTGTTGACGCCGATCAGGAAGTTCTCGAACTCGTTCAGCCCTACGAA
GACAAAGTACAGAAATGGCTCGACCAGCCGGTGGGACTTGCAAAAGGTGATTTCTACATTGATGATCCTTTAATAGCACG
TCTTGCAGATAACCCATTGATAGAATTCATCAATAGAGTCCAGATGTACTACAGCGGTGCCCCGATCTCTTCTTCCGCTC
TCTTCAACAACGATATAAAAGGCTGGAAAGAAGGCCCAATAACACTGAGAGACATTAACGCGGTGTACATATATCCAAAT
ACATTGAAAGTTCTAAGGGTTAAGGGTAAAGACATAAAAGATGCCCTTGAAAAGAGCGCAGATTACTTCGTTTTCGAAGA
TGACCAAATAAAAGTCAACCAATCATGGGTTGATCCCAAACCAAGGCATTATAACTACGACATGTGGGAAGGCATAAACT
ACACGATAGCCGTCAACAGGCCCGTCGGTGACAGAATCGTAGAACTGACATTCCAGGGTAAACCCATCGATATGGAAGCC
GAATACGAAATCGTTCTCAACAATTACAGAGCAGGTGGTGGCGGTGGCTACACCATGTTCAAAGGGAAACCCGTGGTAAG
AGAAGTTATGATGGAAGTTGCCGAACTCATAGCGGATTATGTAATGGAGCACGAAGTCGTGGAGGCACAAACAGATAACA
ATTGGCACGTCGTCGTTGAAAAGGTTCACGAAGTCACCTACGGTGATACGCTCAGCAAGATTGCCAAGATCTATGGAGTA
AGCGTTAAAGACATTCTCAGATGGAATCCCTGGATCGAAAATCCTGATCTTATATACCCGGGTCAAAAAATCACGATATA
CGTTCCATACGTTCGATATATACCGGTACTGTAA

Upstream 100 bases:

>100_bases
AAACCATTCTGTAACACAGCGTTTTTCACAAAACTTTGCTGCTAAACCGTAAATTTGTATAATATACTGTTGTCAAAAAA
ATTGACAAGGGGGAATAAGT

Downstream 100 bases:

>100_bases
CCTGTATTAAATCTTAATAAAAAAAGACAGGGGAGCCGTTTGGCTCCCCTGTCTTTTTTGCTATAAATACTAAGTTCACC
CCAAATTTACACAAATGAAC

Product: 5'-Nucleotidase domain protein

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 597; Mature: 597

Protein sequence:

>597_residues
MRKTFLVFLILLLALSMLAKTVTLTILHTSDLHGNIFPINYATNKPYYVGLGKIQSLVRAVKAENPNILLIDTGDLIQGT
PLEYYHARIDNEPVDPMVLVMNYMGYQCSILGNHEFNYGMNILNKAVSEANFPFLSANIVKKGTEEPYFKPYLITEVDGV
KVGILGLTTKFIPNWEDPKNIAELDFLDPVEVAKKYVKILREEEGVDVVIVGYHGGLERDPKTGEPTEELTGENQGYQLI
EEVPGIDALLTGHQHRSIAEIINGVAVTQPRNWGKALGKIDIVLDDSNGKWEVVDKKVELLSSKTVDADQEVLELVQPYE
DKVQKWLDQPVGLAKGDFYIDDPLIARLADNPLIEFINRVQMYYSGAPISSSALFNNDIKGWKEGPITLRDINAVYIYPN
TLKVLRVKGKDIKDALEKSADYFVFEDDQIKVNQSWVDPKPRHYNYDMWEGINYTIAVNRPVGDRIVELTFQGKPIDMEA
EYEIVLNNYRAGGGGGYTMFKGKPVVREVMMEVAELIADYVMEHEVVEAQTDNNWHVVVEKVHEVTYGDTLSKIAKIYGV
SVKDILRWNPWIENPDLIYPGQKITIYVPYVRYIPVL

Sequences:

>Translated_597_residues
MRKTFLVFLILLLALSMLAKTVTLTILHTSDLHGNIFPINYATNKPYYVGLGKIQSLVRAVKAENPNILLIDTGDLIQGT
PLEYYHARIDNEPVDPMVLVMNYMGYQCSILGNHEFNYGMNILNKAVSEANFPFLSANIVKKGTEEPYFKPYLITEVDGV
KVGILGLTTKFIPNWEDPKNIAELDFLDPVEVAKKYVKILREEEGVDVVIVGYHGGLERDPKTGEPTEELTGENQGYQLI
EEVPGIDALLTGHQHRSIAEIINGVAVTQPRNWGKALGKIDIVLDDSNGKWEVVDKKVELLSSKTVDADQEVLELVQPYE
DKVQKWLDQPVGLAKGDFYIDDPLIARLADNPLIEFINRVQMYYSGAPISSSALFNNDIKGWKEGPITLRDINAVYIYPN
TLKVLRVKGKDIKDALEKSADYFVFEDDQIKVNQSWVDPKPRHYNYDMWEGINYTIAVNRPVGDRIVELTFQGKPIDMEA
EYEIVLNNYRAGGGGGYTMFKGKPVVREVMMEVAELIADYVMEHEVVEAQTDNNWHVVVEKVHEVTYGDTLSKIAKIYGV
SVKDILRWNPWIENPDLIYPGQKITIYVPYVRYIPVL
>Mature_597_residues
MRKTFLVFLILLLALSMLAKTVTLTILHTSDLHGNIFPINYATNKPYYVGLGKIQSLVRAVKAENPNILLIDTGDLIQGT
PLEYYHARIDNEPVDPMVLVMNYMGYQCSILGNHEFNYGMNILNKAVSEANFPFLSANIVKKGTEEPYFKPYLITEVDGV
KVGILGLTTKFIPNWEDPKNIAELDFLDPVEVAKKYVKILREEEGVDVVIVGYHGGLERDPKTGEPTEELTGENQGYQLI
EEVPGIDALLTGHQHRSIAEIINGVAVTQPRNWGKALGKIDIVLDDSNGKWEVVDKKVELLSSKTVDADQEVLELVQPYE
DKVQKWLDQPVGLAKGDFYIDDPLIARLADNPLIEFINRVQMYYSGAPISSSALFNNDIKGWKEGPITLRDINAVYIYPN
TLKVLRVKGKDIKDALEKSADYFVFEDDQIKVNQSWVDPKPRHYNYDMWEGINYTIAVNRPVGDRIVELTFQGKPIDMEA
EYEIVLNNYRAGGGGGYTMFKGKPVVREVMMEVAELIADYVMEHEVVEAQTDNNWHVVVEKVHEVTYGDTLSKIAKIYGV
SVKDILRWNPWIENPDLIYPGQKITIYVPYVRYIPVL

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=558, Percent_Identity=23.4767025089606, Blast_Score=100, Evalue=8e-21,
Organism=Escherichia coli, GI1790658, Length=613, Percent_Identity=32.952691680261, Blast_Score=274, Evalue=1e-74,
Organism=Escherichia coli, GI1786687, Length=548, Percent_Identity=23.7226277372263, Blast_Score=139, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24652512, Length=497, Percent_Identity=24.3460764587525, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19921980, Length=497, Percent_Identity=24.3460764587525, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI161076508, Length=497, Percent_Identity=24.3460764587525, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI28573524, Length=576, Percent_Identity=20.4861111111111, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922446, Length=567, Percent_Identity=22.0458553791887, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24654424, Length=567, Percent_Identity=22.0458553791887, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24641187, Length=512, Percent_Identity=21.6796875, Blast_Score=71, Evalue=3e-12,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 67593; Mature: 67593

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00785 5_NUCLEOTIDASE_1 ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKTFLVFLILLLALSMLAKTVTLTILHTSDLHGNIFPINYATNKPYYVGLGKIQSLVRA
CCHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCEEEEEEECCCCEEEEHHHHHHHHHH
VKAENPNILLIDTGDLIQGTPLEYYHARIDNEPVDPMVLVMNYMGYQCSILGNHEFNYGM
HHCCCCCEEEEECCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCH
NILNKAVSEANFPFLSANIVKKGTEEPYFKPYLITEVDGVKVGILGLTTKFIPNWEDPKN
HHHHHHHHHCCCCEEEHHHHHCCCCCCCCCCEEEEECCCCEEEEEEEEHHCCCCCCCCCC
IAELDFLDPVEVAKKYVKILREEEGVDVVIVGYHGGLERDPKTGEPTEELTGENQGYQLI
CHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHCCCCCCHHHH
EEVPGIDALLTGHQHRSIAEIINGVAVTQPRNWGKALGKIDIVLDDSNGKWEVVDKKVEL
HHCCCCCEECCCCCCHHHHHHHCCEEEECCCHHHHCCCEEEEEEECCCCCEEEHHHHHHH
LSSKTVDADQEVLELVQPYEDKVQKWLDQPVGLAKGDFYIDDPLIARLADNPLIEFINRV
HHCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEECCHHHHHHCCCHHHHHHHHH
QMYYSGAPISSSALFNNDIKGWKEGPITLRDINAVYIYPNTLKVLRVKGKDIKDALEKSA
HHHCCCCCCCCCHHCCCCCCCCCCCCEEEEECCEEEEECCCEEEEEECCCCHHHHHHCCC
DYFVFEDDQIKVNQSWVDPKPRHYNYDMWEGINYTIAVNRPVGDRIVELTFQGKPIDMEA
CEEEEECCEEEEECCCCCCCCCCCCCHHHCCCEEEEEECCCCCCEEEEEEECCCCCCCCC
EYEIVLNNYRAGGGGGYTMFKGKPVVREVMMEVAELIADYVMEHEVVEAQTDNNWHVVVE
CEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEE
KVHEVTYGDTLSKIAKIYGVSVKDILRWNPWIENPDLIYPGQKITIYVPYVRYIPVL
HHHHHHCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEECCCEEEEEECHHHHCCCC
>Mature Secondary Structure
MRKTFLVFLILLLALSMLAKTVTLTILHTSDLHGNIFPINYATNKPYYVGLGKIQSLVRA
CCHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCEEEEEEECCCCEEEEHHHHHHHHHH
VKAENPNILLIDTGDLIQGTPLEYYHARIDNEPVDPMVLVMNYMGYQCSILGNHEFNYGM
HHCCCCCEEEEECCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCCH
NILNKAVSEANFPFLSANIVKKGTEEPYFKPYLITEVDGVKVGILGLTTKFIPNWEDPKN
HHHHHHHHHCCCCEEEHHHHHCCCCCCCCCCEEEEECCCCEEEEEEEEHHCCCCCCCCCC
IAELDFLDPVEVAKKYVKILREEEGVDVVIVGYHGGLERDPKTGEPTEELTGENQGYQLI
CHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCHHHHCCCCCCHHHH
EEVPGIDALLTGHQHRSIAEIINGVAVTQPRNWGKALGKIDIVLDDSNGKWEVVDKKVEL
HHCCCCCEECCCCCCHHHHHHHCCEEEECCCHHHHCCCEEEEEEECCCCCEEEHHHHHHH
LSSKTVDADQEVLELVQPYEDKVQKWLDQPVGLAKGDFYIDDPLIARLADNPLIEFINRV
HHCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCEEECCHHHHHHCCCHHHHHHHHH
QMYYSGAPISSSALFNNDIKGWKEGPITLRDINAVYIYPNTLKVLRVKGKDIKDALEKSA
HHHCCCCCCCCCHHCCCCCCCCCCCCEEEEECCEEEEECCCEEEEEECCCCHHHHHHCCC
DYFVFEDDQIKVNQSWVDPKPRHYNYDMWEGINYTIAVNRPVGDRIVELTFQGKPIDMEA
CEEEEECCEEEEECCCCCCCCCCCCCHHHCCCEEEEEECCCCCCEEEEEEECCCCCCCCC
EYEIVLNNYRAGGGGGYTMFKGKPVVREVMMEVAELIADYVMEHEVVEAQTDNNWHVVVE
CEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEE
KVHEVTYGDTLSKIAKIYGVSVKDILRWNPWIENPDLIYPGQKITIYVPYVRYIPVL
HHHHHHCCHHHHHHHHHHCCCHHHHHHCCCCCCCCCEEECCCEEEEEECHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]