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The map label for this gene is pgk [H]

Identifier: 239617385

GI number: 239617385

Start: 1051030

End: 1052295

Strand: Reverse

Name: pgk [H]

Synonym: Kole_0995

Alternate gene names: 239617385

Gene position: 1052295-1051030 (Counterclockwise)

Preceding gene: 239617386

Following gene: 239617384

Centisome position: 45.71

GC content: 42.34

Gene sequence:

>1266_bases
ATGAGGGAGCTTCCTATCAATACTCTCGATGATTTCGATTTTAACAACAAGACCGTGATACTGAGAATAGACATAAACTC
TCCCATAGATCCAAAAACAAAGAAAATCGTTAACGAAAATAGGCTAAATAAAAGCATTCCAACCATAAAAACTTTGATGG
AGAAAGGCGCCAAAATTGCAATTCTTGCCCATCAGGGTGATACACTCGATTATCACAACCTGATACCAATGAACGAACAT
GCCGAGAAACTTTCCGAAAAACTTGGAAAGGAAGTCAAGTACATCGATGATGTTGCCGGACCTGCCGCTCAGGAAGCTGT
TAAAAACTTAAAGCCAGGAGAAGCGATTCTTCTCGGTAATGTTCGCTATCTTACTGAAGAAGTTTCTACCTTTGAAAACG
TCGTGAAATTAGAACCTCATGAAATGTTGAATACCTACATAGTAAGAAATCTCGCACCGCTTGCTGATTACTATGTCAAC
GATGCTTTTTCTGCTGCCCACAGAAATTCTCCTTCTATGGTTGCCTTTGAAGAGATTCTCCCAACTGCTGCTGGAGAACT
GCTTTTCAAAGAAGTATCCGCACTAACAAGAGTCAGGGAAAATCCGGATAAACCAGCGGTGTTCGTTCTGGGGGGCCTGA
AGATATCAGATGCTTTCGGTATGATGAAGGAAGTTCTCGAAAACGGTACAGCAGACAAAATCCTCACTTGTGGGGTCACC
GGTATCGTTATGCAGATGGCGATGGGGAAAAAACTTGGAAAGGCCCAGGAAAAATTCATCTATGACCGGTCACTCGATGT
ATTCATAAAACCCGCTAAAGAGTATATGGAAAAATTTGGAGACAAATTTCTCAATCCGGTGGACTTCGCTTACGAGAGAG
ATGGAAAAAGGGTGGAATGTTCGATCGATGATCTCCCAATAGATGATGTGCTTTTCATGGATATTGGAACCAAAACGATA
GAGCTTTATAAAGAGGAGATTAATAGAGCTGGTACTATCTTCGTTAATGGTCCAGCTGGTGCCTACGAAAACCCAATCTT
TGAAAAGGGAACACGGGAAATCTGGAACGCAATAGCTGATGCTCCTGGTTACAGCGTCGTTGGAGGCGGCGACAGCGTTA
CAGCCGCCCAAAAACTTGTAGAAAATGCCGAAAAAAAGTTCGGCTACATCTGTACGGCAGGCGGGGCAATGGTTAGATTC
CTTTCTGGAAAAGAACTTCCTCTTATAAAAGCTTTGAAAAAAGCTTTTAGGAAGGTTGAAAAATGA

Upstream 100 bases:

>100_bases
AGACGATAGATGGTATCAGAGCAGCCATGGAAATGCAAACAGACAGACTTGAGGCTGTTGGGCTCACAAACAAATACTTG
AAGATAGGGGGATAGTTTCC

Downstream 100 bases:

>100_bases
TACGTGAGTTTTCCCTCCCTTATGGCAAGAGGCAATTACCGGTTAAATTGCCTCTTGCTGGGTTTGCAGGTGAGTTTTTG
CCAATAAGGCACGTTTCAGG

Product: Phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MRELPINTLDDFDFNNKTVILRIDINSPIDPKTKKIVNENRLNKSIPTIKTLMEKGAKIAILAHQGDTLDYHNLIPMNEH
AEKLSEKLGKEVKYIDDVAGPAAQEAVKNLKPGEAILLGNVRYLTEEVSTFENVVKLEPHEMLNTYIVRNLAPLADYYVN
DAFSAAHRNSPSMVAFEEILPTAAGELLFKEVSALTRVRENPDKPAVFVLGGLKISDAFGMMKEVLENGTADKILTCGVT
GIVMQMAMGKKLGKAQEKFIYDRSLDVFIKPAKEYMEKFGDKFLNPVDFAYERDGKRVECSIDDLPIDDVLFMDIGTKTI
ELYKEEINRAGTIFVNGPAGAYENPIFEKGTREIWNAIADAPGYSVVGGGDSVTAAQKLVENAEKKFGYICTAGGAMVRF
LSGKELPLIKALKKAFRKVEK

Sequences:

>Translated_421_residues
MRELPINTLDDFDFNNKTVILRIDINSPIDPKTKKIVNENRLNKSIPTIKTLMEKGAKIAILAHQGDTLDYHNLIPMNEH
AEKLSEKLGKEVKYIDDVAGPAAQEAVKNLKPGEAILLGNVRYLTEEVSTFENVVKLEPHEMLNTYIVRNLAPLADYYVN
DAFSAAHRNSPSMVAFEEILPTAAGELLFKEVSALTRVRENPDKPAVFVLGGLKISDAFGMMKEVLENGTADKILTCGVT
GIVMQMAMGKKLGKAQEKFIYDRSLDVFIKPAKEYMEKFGDKFLNPVDFAYERDGKRVECSIDDLPIDDVLFMDIGTKTI
ELYKEEINRAGTIFVNGPAGAYENPIFEKGTREIWNAIADAPGYSVVGGGDSVTAAQKLVENAEKKFGYICTAGGAMVRF
LSGKELPLIKALKKAFRKVEK
>Mature_421_residues
MRELPINTLDDFDFNNKTVILRIDINSPIDPKTKKIVNENRLNKSIPTIKTLMEKGAKIAILAHQGDTLDYHNLIPMNEH
AEKLSEKLGKEVKYIDDVAGPAAQEAVKNLKPGEAILLGNVRYLTEEVSTFENVVKLEPHEMLNTYIVRNLAPLADYYVN
DAFSAAHRNSPSMVAFEEILPTAAGELLFKEVSALTRVRENPDKPAVFVLGGLKISDAFGMMKEVLENGTADKILTCGVT
GIVMQMAMGKKLGKAQEKFIYDRSLDVFIKPAKEYMEKFGDKFLNPVDFAYERDGKRVECSIDDLPIDDVLFMDIGTKTI
ELYKEEINRAGTIFVNGPAGAYENPIFEKGTREIWNAIADAPGYSVVGGGDSVTAAQKLVENAEKKFGYICTAGGAMVRF
LSGKELPLIKALKKAFRKVEK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=420, Percent_Identity=30.4761904761905, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI31543397, Length=423, Percent_Identity=30.9692671394799, Blast_Score=176, Evalue=4e-44,
Organism=Escherichia coli, GI1789294, Length=419, Percent_Identity=30.0715990453461, Blast_Score=150, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17508823, Length=429, Percent_Identity=31.7016317016317, Blast_Score=188, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6319857, Length=415, Percent_Identity=31.3253012048193, Blast_Score=172, Evalue=7e-44,
Organism=Drosophila melanogaster, GI17136394, Length=423, Percent_Identity=30.2600472813239, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24581203, Length=423, Percent_Identity=30.7328605200946, Blast_Score=166, Evalue=2e-41,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 46705; Mature: 46705

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00111 PGLYCERATE_KINASE ; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRELPINTLDDFDFNNKTVILRIDINSPIDPKTKKIVNENRLNKSIPTIKTLMEKGAKIA
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCEEE
ILAHQGDTLDYHNLIPMNEHAEKLSEKLGKEVKYIDDVAGPAAQEAVKNLKPGEAILLGN
EEEECCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHCCCCCCEEEECC
VRYLTEEVSTFENVVKLEPHEMLNTYIVRNLAPLADYYVNDAFSAAHRNSPSMVAFEEIL
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
PTAAGELLFKEVSALTRVRENPDKPAVFVLGGLKISDAFGMMKEVLENGTADKILTCGVT
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCEECCCHH
GIVMQMAMGKKLGKAQEKFIYDRSLDVFIKPAKEYMEKFGDKFLNPVDFAYERDGKRVEC
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEE
SIDDLPIDDVLFMDIGTKTIELYKEEINRAGTIFVNGPAGAYENPIFEKGTREIWNAIAD
ECCCCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHC
APGYSVVGGGDSVTAAQKLVENAEKKFGYICTAGGAMVRFLSGKELPLIKALKKAFRKVE
CCCCEEECCCCHHHHHHHHHHHHHHHCCEEEECCHHHHHHHCCCCCHHHHHHHHHHHHHC
K
C
>Mature Secondary Structure
MRELPINTLDDFDFNNKTVILRIDINSPIDPKTKKIVNENRLNKSIPTIKTLMEKGAKIA
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCEEE
ILAHQGDTLDYHNLIPMNEHAEKLSEKLGKEVKYIDDVAGPAAQEAVKNLKPGEAILLGN
EEEECCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHCCCCCCEEEECC
VRYLTEEVSTFENVVKLEPHEMLNTYIVRNLAPLADYYVNDAFSAAHRNSPSMVAFEEIL
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
PTAAGELLFKEVSALTRVRENPDKPAVFVLGGLKISDAFGMMKEVLENGTADKILTCGVT
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCEECCCHH
GIVMQMAMGKKLGKAQEKFIYDRSLDVFIKPAKEYMEKFGDKFLNPVDFAYERDGKRVEC
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCHHHHHHCCCCEEEE
SIDDLPIDDVLFMDIGTKTIELYKEEINRAGTIFVNGPAGAYENPIFEKGTREIWNAIAD
ECCCCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHC
APGYSVVGGGDSVTAAQKLVENAEKKFGYICTAGGAMVRFLSGKELPLIKALKKAFRKVE
CCCCEEECCCCHHHHHHHHHHHHHHHCCEEEECCHHHHHHHCCCCCHHHHHHHHHHHHHC
K
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]