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The map label for this gene is rne [H]

Identifier: 239616948

GI number: 239616948

Start: 596551

End: 597996

Strand: Direct

Name: rne [H]

Synonym: Kole_0546

Alternate gene names: 239616948

Gene position: 596551-597996 (Clockwise)

Preceding gene: 239616947

Following gene: 239616949

Centisome position: 25.91

GC content: 39.0

Gene sequence:

>1446_bases
ATGGAGATCACAAAAAAGAAAAGAACGCAGGCTATCGTTTTTAGCTCATTAGAAGATGAGGTAAGGATCGCCATTCTTGA
CGAAGGTAAAATCCAGGAAGTTTTTTTTGAAGAAATGGACAACGAAAGTTACATGGGAAACATTTATCTTGGAAAGATAG
AAAATGTGGTTCCAAGTTTGGAAGCAGCTTTTGTTAATATCGGAATCGGGAAAAACGGCTTCCTTAGATTCAAAGATACC
AGAAATAACCAAAAACTTGAAAAAGGAAAAAAAGTCCTAGTCCAGGTTAAAAAAGATCCAATAGGTTCCAAAGGTCCACA
ACTAACACTGAAAATCAGTATACCAGGAAGATGTCTCGTTTATATGCCCTTTTCCAAACACGTAGGAATATCGAAAAAAA
TAACCGACCAGGCAGAACGTGAAAGGCTCATCTCCATCGCTAAAGGAATAATAGAGAATGATGAAGGGATTATCTTCAGA
ACAGCCGCTAACGGTGTTGGTGAAGATACTCTTCGCGAAGAGCTGGAAAACCTGAGAACAATATGGAAAAAGATAAACGA
AAAATTCAGAAGGGCGAGAAAACCCCAGATTCTCTATGAAGAACCAAGTCTTGTTGAATACATACTTAGAGAGAGACTAA
CTGAAGATGTAAAAGAAATAGTCGTCGATAGCGAAGATCTCTGGCACGATGTTGTTGCAGGGATAAGCAAATTCAAAAGC
GGGTTTAAACCGATTGTAAGGTACGTTGAAGAAGACGCATTCCTACATGAAGGAATATACGAAAAGATGAAGGTATTATA
CACACGGGTCGTTCCACTTCCAAATGGCGGAAATATATTCATAGATAAAACAGAAGCAATGACAGTCATCGATGTTAACT
CAGCCAGCAATACTCACGGCAGTGACGTTAGTGAAATGTCTTTGAACACTAATCTTGAAGCCGCGGTAGAGATAGCGCGA
CAGCTACGCTTGAGAAACATTGGTGGAATTATAGTCATTGACTTTATCGATATGAAAAGTGAAGCTGACAGAAAGAAGGT
TATCTCGCTGCTTTCTGAAGAACTTAAAAAAGACAAGGCGCATTCACTGATCCTTGGATTTACAAGACTCGGTCTTCTTG
AAATGACCCGTAAGAGATCAACAGCAACTATAAATTCCAGAATATATAGTACATGCCCTATATGTCGTGGAACTGGTCAA
ATAGAGTCACCAAAAATTACTTACCAACGGCTCGTAAGCGACTTACAGAGATTGTTCAAAGACAAGAAAATCAAATCAGC
TACCGTGCAGGTATATCAAAATCTTTCTGGAATACTCACACCTGAGGTACAAAAGCAGTTGAGTAAGCAATTCAAGAGAG
AAATTAAAGTGTCCTTCACCTGGCCAGTCCCAGCGACTTACGATATTAAAGGAAGTACAAAGTCAGAAGCAAAGTCAAAA
AAATAG

Upstream 100 bases:

>100_bases
ACAGTTGGTTTAATAGAAATGTCAGAATAATCAAAAAATTTGAACTACCTGATCAGGCCGCTTTTTGCGGCCTGGTTTTA
AACAAATAAAGTGGAGGTGA

Downstream 100 bases:

>100_bases
GGGTTTGGAAGATTATCCGATTAAACAATGGCGTGCCCGGGGCGATTCGAACGCCCGGCCTCCAGATCCGCAGTCTAGCG
CTCTATCCAGCTGAGCTACG

Product: ribonuclease, Rne/Rng family

Products: NA

Alternate protein names: RNase E [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MEITKKKRTQAIVFSSLEDEVRIAILDEGKIQEVFFEEMDNESYMGNIYLGKIENVVPSLEAAFVNIGIGKNGFLRFKDT
RNNQKLEKGKKVLVQVKKDPIGSKGPQLTLKISIPGRCLVYMPFSKHVGISKKITDQAERERLISIAKGIIENDEGIIFR
TAANGVGEDTLREELENLRTIWKKINEKFRRARKPQILYEEPSLVEYILRERLTEDVKEIVVDSEDLWHDVVAGISKFKS
GFKPIVRYVEEDAFLHEGIYEKMKVLYTRVVPLPNGGNIFIDKTEAMTVIDVNSASNTHGSDVSEMSLNTNLEAAVEIAR
QLRLRNIGGIIVIDFIDMKSEADRKKVISLLSEELKKDKAHSLILGFTRLGLLEMTRKRSTATINSRIYSTCPICRGTGQ
IESPKITYQRLVSDLQRLFKDKKIKSATVQVYQNLSGILTPEVQKQLSKQFKREIKVSFTWPVPATYDIKGSTKSEAKSK
K

Sequences:

>Translated_481_residues
MEITKKKRTQAIVFSSLEDEVRIAILDEGKIQEVFFEEMDNESYMGNIYLGKIENVVPSLEAAFVNIGIGKNGFLRFKDT
RNNQKLEKGKKVLVQVKKDPIGSKGPQLTLKISIPGRCLVYMPFSKHVGISKKITDQAERERLISIAKGIIENDEGIIFR
TAANGVGEDTLREELENLRTIWKKINEKFRRARKPQILYEEPSLVEYILRERLTEDVKEIVVDSEDLWHDVVAGISKFKS
GFKPIVRYVEEDAFLHEGIYEKMKVLYTRVVPLPNGGNIFIDKTEAMTVIDVNSASNTHGSDVSEMSLNTNLEAAVEIAR
QLRLRNIGGIIVIDFIDMKSEADRKKVISLLSEELKKDKAHSLILGFTRLGLLEMTRKRSTATINSRIYSTCPICRGTGQ
IESPKITYQRLVSDLQRLFKDKKIKSATVQVYQNLSGILTPEVQKQLSKQFKREIKVSFTWPVPATYDIKGSTKSEAKSK
K
>Mature_481_residues
MEITKKKRTQAIVFSSLEDEVRIAILDEGKIQEVFFEEMDNESYMGNIYLGKIENVVPSLEAAFVNIGIGKNGFLRFKDT
RNNQKLEKGKKVLVQVKKDPIGSKGPQLTLKISIPGRCLVYMPFSKHVGISKKITDQAERERLISIAKGIIENDEGIIFR
TAANGVGEDTLREELENLRTIWKKINEKFRRARKPQILYEEPSLVEYILRERLTEDVKEIVVDSEDLWHDVVAGISKFKS
GFKPIVRYVEEDAFLHEGIYEKMKVLYTRVVPLPNGGNIFIDKTEAMTVIDVNSASNTHGSDVSEMSLNTNLEAAVEIAR
QLRLRNIGGIIVIDFIDMKSEADRKKVISLLSEELKKDKAHSLILGFTRLGLLEMTRKRSTATINSRIYSTCPICRGTGQ
IESPKITYQRLVSDLQRLFKDKKIKSATVQVYQNLSGILTPEVQKQLSKQFKREIKVSFTWPVPATYDIKGSTKSEAKSK
K

Specific function: Involved In The Processing Of The 5'End Of 16s Rrna. Could Be Involved In Chromosome Segregation And Cell Division. It May Be One Of The Components Of The Cytoplasmic Axial Filaments Bundles Or Merely Regulate The Formation Of This Structure. [C]

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI87082235, Length=472, Percent_Identity=33.8983050847458, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1787325, Length=443, Percent_Identity=34.7629796839729, Blast_Score=241, Evalue=1e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: =3.1.26.12 [H]

Molecular weight: Translated: 54650; Mature: 54650

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEITKKKRTQAIVFSSLEDEVRIAILDEGKIQEVFFEEMDNESYMGNIYLGKIENVVPSL
CCCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCCEECEEEECCHHHHHHCH
EAAFVNIGIGKNGFLRFKDTRNNQKLEKGKKVLVQVKKDPIGSKGPQLTLKISIPGRCLV
HHHEEEEECCCCCCEEEECCCCCHHHHHHHHEEEEEECCCCCCCCCEEEEEEECCCCEEE
YMPFSKHVGISKKITDQAERERLISIAKGIIENDEGIIFRTAANGVGEDTLREELENLRT
EECCHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHH
IWKKINEKFRRARKPQILYEEPSLVEYILRERLTEDVKEIVVDSEDLWHDVVAGISKFKS
HHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
GFKPIVRYVEEDAFLHEGIYEKMKVLYTRVVPLPNGGNIFIDKTEAMTVIDVNSASNTHG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCEEEEEEECCCCCCCC
SDVSEMSLNTNLEAAVEIARQLRLRNIGGIIVIDFIDMKSEADRKKVISLLSEELKKDKA
CCHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
HSLILGFTRLGLLEMTRKRSTATINSRIYSTCPICRGTGQIESPKITYQRLVSDLQRLFK
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
DKKIKSATVQVYQNLSGILTPEVQKQLSKQFKREIKVSFTWPVPATYDIKGSTKSEAKSK
HCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEEEECCCCEEEECCCCCCCHHCCC
K
C
>Mature Secondary Structure
MEITKKKRTQAIVFSSLEDEVRIAILDEGKIQEVFFEEMDNESYMGNIYLGKIENVVPSL
CCCCHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHCCCCCEECEEEECCHHHHHHCH
EAAFVNIGIGKNGFLRFKDTRNNQKLEKGKKVLVQVKKDPIGSKGPQLTLKISIPGRCLV
HHHEEEEECCCCCCEEEECCCCCHHHHHHHHEEEEEECCCCCCCCCEEEEEEECCCCEEE
YMPFSKHVGISKKITDQAERERLISIAKGIIENDEGIIFRTAANGVGEDTLREELENLRT
EECCHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHH
IWKKINEKFRRARKPQILYEEPSLVEYILRERLTEDVKEIVVDSEDLWHDVVAGISKFKS
HHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
GFKPIVRYVEEDAFLHEGIYEKMKVLYTRVVPLPNGGNIFIDKTEAMTVIDVNSASNTHG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCEEEEEEECCCCCCCC
SDVSEMSLNTNLEAAVEIARQLRLRNIGGIIVIDFIDMKSEADRKKVISLLSEELKKDKA
CCHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
HSLILGFTRLGLLEMTRKRSTATINSRIYSTCPICRGTGQIESPKITYQRLVSDLQRLFK
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
DKKIKSATVQVYQNLSGILTPEVQKQLSKQFKREIKVSFTWPVPATYDIKGSTKSEAKSK
HCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEEEECCCCEEEECCCCCCCHHCCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]