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The map label for this gene is thiH [C]

Identifier: 239616803

GI number: 239616803

Start: 414775

End: 415866

Strand: Direct

Name: thiH [C]

Synonym: Kole_0396

Alternate gene names: 239616803

Gene position: 414775-415866 (Clockwise)

Preceding gene: 239616802

Following gene: 239616804

Centisome position: 18.02

GC content: 39.93

Gene sequence:

>1092_bases
ATGGCGAGTAGTAGATTTGATATCGCGAAAAAAATTGAGGTTGAAGGTTCTAAAGAGCTACAGAGAATCGTCAAGAAAGT
TCTAACAGAAGAAGAACTATCAAAAGAAGAGATAATCTATATCCTGTCGCTAGAAAGAGGGGAAAAAGACAGGGAACTGC
TCTTTGAATTCTCAAATGCTATTAGAAAAGACTACGTCGGTGAGATGTTTTTCATAAAAGGAGTTATAGAGTTTTCCAAC
TACTGTCGAAAAAACTGTGACTACTGCGGGATACGTGCTGCAAATAAAATCCCCCGTTACAGAATGTCAGAAAAAGAAAT
CATAGAGATTGCAAAAACGATGCTTCCCATTGGAGTGGATACGATAATACTTCAATCTGGAGAAGATCCTTACTACGATA
TAGAAAAACTGGAAAGGATTGTCTCACGTATCAAAAAAGAAACACATCTCCCTGTTTCTCTCTCCATAGGCGAAAGACCA
AAAACCGAATACTTAAAGCTCAAAGCTGCAGGCGCCAGCAAAACCTTGTTGAAACACGAAACGATGAACAGGAAAATATT
CGAAAGTGTGCATCCTGATGATGATTACGACAAGCGGATAGACCTCCTTCTCTACACTGTCTCTATCGGATATATTGCCG
GTTCAGGAAATATAATAGGCCTTCCAGGACAAACCATAGAAGATGTAGCCGATGACGTTCTTTTCTTGAGGGATTCCGGC
GTCAGGATGGTCGGCCTTGGGCCTTTTATACCAGCCAAAGGAACACCTCTCGAAAATCATCCTTACGGAGACCCTGAACT
CACTTTGAATACCTATGCAGCTGTAAGATTATGCATTCCAAAGGTTTTCATGCCGTCCACCACAGCGTTGGGAACTATCG
ATGAAGACCTGCAATTTGAAGGATTCAAAGTTGGTTGTAACGTTATAATGTGCAATTTCACACCGGAAAAGTACAGGAAA
AACTACACGATTTACTCTGATAAAATCAAAGTTGATTTCTTTAAAACAGCAAAAAGAATGAAAGATATGGGCCTCAAGCT
AAATCCAAGGATTCTGAAAAACCTTGAGGAATACAAGAAGGTGATCATATGA

Upstream 100 bases:

>100_bases
GTTTTGGAGTTTGGAAAATACGCGGTTTTCGCCGAAAACCTATCGGAAGTTCAGAAAGAAAAACTACAGCAGGAAATTAT
GCGAAAGTTGAGGGAAAATG

Downstream 100 bases:

>100_bases
TTGCTACCGGTGGTTATAGAAAGTATCTTGCATTCTTCGGAAAGCGAAACGTTGGAAAGTCGAGTTTGATAAACGCTCTC
GTTGGACAGGATGTGTCTAT

Product: Radical SAM domain protein

Products: biotin

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MASSRFDIAKKIEVEGSKELQRIVKKVLTEEELSKEEIIYILSLERGEKDRELLFEFSNAIRKDYVGEMFFIKGVIEFSN
YCRKNCDYCGIRAANKIPRYRMSEKEIIEIAKTMLPIGVDTIILQSGEDPYYDIEKLERIVSRIKKETHLPVSLSIGERP
KTEYLKLKAAGASKTLLKHETMNRKIFESVHPDDDYDKRIDLLLYTVSIGYIAGSGNIIGLPGQTIEDVADDVLFLRDSG
VRMVGLGPFIPAKGTPLENHPYGDPELTLNTYAAVRLCIPKVFMPSTTALGTIDEDLQFEGFKVGCNVIMCNFTPEKYRK
NYTIYSDKIKVDFFKTAKRMKDMGLKLNPRILKNLEEYKKVII

Sequences:

>Translated_363_residues
MASSRFDIAKKIEVEGSKELQRIVKKVLTEEELSKEEIIYILSLERGEKDRELLFEFSNAIRKDYVGEMFFIKGVIEFSN
YCRKNCDYCGIRAANKIPRYRMSEKEIIEIAKTMLPIGVDTIILQSGEDPYYDIEKLERIVSRIKKETHLPVSLSIGERP
KTEYLKLKAAGASKTLLKHETMNRKIFESVHPDDDYDKRIDLLLYTVSIGYIAGSGNIIGLPGQTIEDVADDVLFLRDSG
VRMVGLGPFIPAKGTPLENHPYGDPELTLNTYAAVRLCIPKVFMPSTTALGTIDEDLQFEGFKVGCNVIMCNFTPEKYRK
NYTIYSDKIKVDFFKTAKRMKDMGLKLNPRILKNLEEYKKVII
>Mature_362_residues
ASSRFDIAKKIEVEGSKELQRIVKKVLTEEELSKEEIIYILSLERGEKDRELLFEFSNAIRKDYVGEMFFIKGVIEFSNY
CRKNCDYCGIRAANKIPRYRMSEKEIIEIAKTMLPIGVDTIILQSGEDPYYDIEKLERIVSRIKKETHLPVSLSIGERPK
TEYLKLKAAGASKTLLKHETMNRKIFESVHPDDDYDKRIDLLLYTVSIGYIAGSGNIIGLPGQTIEDVADDVLFLRDSGV
RMVGLGPFIPAKGTPLENHPYGDPELTLNTYAAVRLCIPKVFMPSTTALGTIDEDLQFEGFKVGCNVIMCNFTPEKYRKN
YTIYSDKIKVDFFKTAKRMKDMGLKLNPRILKNLEEYKKVII

Specific function: Required For The Synthesis Of The Thiazole Moiety Of Thiamine. [C]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: 2.8.1.6

Molecular weight: Translated: 41516; Mature: 41385

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASSRFDIAKKIEVEGSKELQRIVKKVLTEEELSKEEIIYILSLERGEKDRELLFEFSNA
CCCCCCCCHHEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHH
IRKDYVGEMFFIKGVIEFSNYCRKNCDYCGIRAANKIPRYRMSEKEIIEIAKTMLPIGVD
HHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHCCCCE
TIILQSGEDPYYDIEKLERIVSRIKKETHLPVSLSIGERPKTEYLKLKAAGASKTLLKHE
EEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEECCCCHHHHHHH
TMNRKIFESVHPDDDYDKRIDLLLYTVSIGYIAGSGNIIGLPGQTIEDVADDVLFLRDSG
HHHHHHHHHCCCCCCHHHHHEEEEEEEEEEEEECCCCEEECCCCHHHHHHHHEEEEECCC
VRMVGLGPFIPAKGTPLENHPYGDPELTLNTYAAVRLCIPKVFMPSTTALGTIDEDLQFE
EEEEECCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
GFKVGCNVIMCNFTPEKYRKNYTIYSDKIKVDFFKTAKRMKDMGLKLNPRILKNLEEYKK
CEEEECEEEEECCCHHHHCCCCEEEECEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
VII
HCC
>Mature Secondary Structure 
ASSRFDIAKKIEVEGSKELQRIVKKVLTEEELSKEEIIYILSLERGEKDRELLFEFSNA
CCCCCCCHHEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHH
IRKDYVGEMFFIKGVIEFSNYCRKNCDYCGIRAANKIPRYRMSEKEIIEIAKTMLPIGVD
HHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHCCCCE
TIILQSGEDPYYDIEKLERIVSRIKKETHLPVSLSIGERPKTEYLKLKAAGASKTLLKHE
EEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEECCCCHHHHHHH
TMNRKIFESVHPDDDYDKRIDLLLYTVSIGYIAGSGNIIGLPGQTIEDVADDVLFLRDSG
HHHHHHHHHCCCCCCHHHHHEEEEEEEEEEEEECCCCEEECCCCHHHHHHHHEEEEECCC
VRMVGLGPFIPAKGTPLENHPYGDPELTLNTYAAVRLCIPKVFMPSTTALGTIDEDLQFE
EEEEECCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
GFKVGCNVIMCNFTPEKYRKNYTIYSDKIKVDFFKTAKRMKDMGLKLNPRILKNLEEYKK
CEEEECEEEEECCCHHHHCCCCEEEECEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
VII
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dethiobiotin; sulfur

Specific reaction: dethiobiotin + sulfur = biotin

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]