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The map label for this gene is ahpF [H]

Identifier: 239616714

GI number: 239616714

Start: 333381

End: 334028

Strand: Reverse

Name: ahpF [H]

Synonym: Kole_0307

Alternate gene names: 239616714

Gene position: 334028-333381 (Counterclockwise)

Preceding gene: 239616715

Following gene: 239616713

Centisome position: 14.51

GC content: 41.36

Gene sequence:

>648_bases
ATGTCGGAAAAATTATTGAACGATGAAATCAGAACGCAAGTAAAGGATATACTGTCAGAGATGGAAGGAAAGGTGAAGCT
CCTGTTTTTTGAGAACAAGGACAATTGCGATTACTGTTCGACGATCGAACAACTCCTCAGTGAGCTCACTGAAGTTACCG
ATATGGTTGTATTTGAAAAGTATGATGCCGATTCTGAAAAGGCAAAGGAATATTCCATTGAGATTTCTCCGGCTATCGTG
CTTTTAACTCCAGACGGAGTTGATAAAGGTGTAAGGTTCTATGGGATACCTTCTGGACATGAGTTTGGAACGTTGCTTCA
GGATATAATTAGTTTCTCTAAAGGAGCGAAGCCCAATCTTTCTCCGGAGTCCATTGAGAGATTGAAGACGATTGAAAAAC
CCATAGACATAAAGGTTTTCGTAACACCCACGTGCCCGTATTGTCCACGCGCTGTTATCACAGCTCACAATATCGCTATG
GCAAACGAAAACGTTGTTGCTTCCATGATAGAGGCCAATGAATTCCCTGATTTGTCGATGAAGTTTGGGGTTAGTTCGGT
ACCTCAGATAGTTATCAACGATGAGGTCATTTTTGTTGGAGCTTATCCAGAAAAACAGTTTATAGACGAAGTCTTTAAGG
CGGTTTGA

Upstream 100 bases:

>100_bases
GGAAGATTTTGTAAACAAAGAATCAAAATCCGAGTAACATAGTCGTGTTATCATACCCATACAGGGTATACTGAGTTAAC
CAAAAAAGGAGGAAAATAGA

Downstream 100 bases:

>100_bases
TAAGGAGGGATTCCTGTGGCCTTCTTCGATCTTGGAAGTGCTAAACAAAAGAGCGAAGTAAAGGATTACTACGATATACT
CATCATTGGAGGAGGTCCGG

Product: glutaredoxin-like domain protein

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MSEKLLNDEIRTQVKDILSEMEGKVKLLFFENKDNCDYCSTIEQLLSELTEVTDMVVFEKYDADSEKAKEYSIEISPAIV
LLTPDGVDKGVRFYGIPSGHEFGTLLQDIISFSKGAKPNLSPESIERLKTIEKPIDIKVFVTPTCPYCPRAVITAHNIAM
ANENVVASMIEANEFPDLSMKFGVSSVPQIVINDEVIFVGAYPEKQFIDEVFKAV

Sequences:

>Translated_215_residues
MSEKLLNDEIRTQVKDILSEMEGKVKLLFFENKDNCDYCSTIEQLLSELTEVTDMVVFEKYDADSEKAKEYSIEISPAIV
LLTPDGVDKGVRFYGIPSGHEFGTLLQDIISFSKGAKPNLSPESIERLKTIEKPIDIKVFVTPTCPYCPRAVITAHNIAM
ANENVVASMIEANEFPDLSMKFGVSSVPQIVINDEVIFVGAYPEKQFIDEVFKAV
>Mature_214_residues
SEKLLNDEIRTQVKDILSEMEGKVKLLFFENKDNCDYCSTIEQLLSELTEVTDMVVFEKYDADSEKAKEYSIEISPAIVL
LTPDGVDKGVRFYGIPSGHEFGTLLQDIISFSKGAKPNLSPESIERLKTIEKPIDIKVFVTPTCPYCPRAVITAHNIAMA
NENVVASMIEANEFPDLSMKFGVSSVPQIVINDEVIFVGAYPEKQFIDEVFKAV

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=190, Percent_Identity=24.2105263157895, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 24040; Mature: 23909

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKLLNDEIRTQVKDILSEMEGKVKLLFFENKDNCDYCSTIEQLLSELTEVTDMVVFEK
CCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHC
YDADSEKAKEYSIEISPAIVLLTPDGVDKGVRFYGIPSGHEFGTLLQDIISFSKGAKPNL
CCCCCHHHHHEEEEECCEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCC
SPESIERLKTIEKPIDIKVFVTPTCPYCPRAVITAHNIAMANENVVASMIEANEFPDLSM
CHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHEECCCHHHHHHHHHCCCCCCHH
KFGVSSVPQIVINDEVIFVGAYPEKQFIDEVFKAV
HCCCCCCCEEEECCCEEEEECCCCHHHHHHHHHHC
>Mature Secondary Structure 
SEKLLNDEIRTQVKDILSEMEGKVKLLFFENKDNCDYCSTIEQLLSELTEVTDMVVFEK
CCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHC
YDADSEKAKEYSIEISPAIVLLTPDGVDKGVRFYGIPSGHEFGTLLQDIISFSKGAKPNL
CCCCCHHHHHEEEEECCEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCC
SPESIERLKTIEKPIDIKVFVTPTCPYCPRAVITAHNIAMANENVVASMIEANEFPDLSM
CHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHEECCCHHHHHHHHHCCCCCCHH
KFGVSSVPQIVINDEVIFVGAYPEKQFIDEVFKAV
HCCCCCCCEEEECCCEEEEECCCCHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA