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The map label for this gene is ansA [H]

Identifier: 239616655

GI number: 239616655

Start: 276724

End: 277704

Strand: Reverse

Name: ansA [H]

Synonym: Kole_0248

Alternate gene names: 239616655

Gene position: 277704-276724 (Counterclockwise)

Preceding gene: 239616656

Following gene: 239616654

Centisome position: 12.06

GC content: 42.41

Gene sequence:

>981_bases
TTGCCTGAAATCGCGATAATTACAACCGGAGGTACCATCGCAATGGTAAGGGATCCATCAACAGGTGCTTCTGTTCCCGC
AAGTGGAATGAACGTTCATGTGGCAGAAGTTGACGGATTATCTGCAATCGCAAAGATACGACATTACGATTTCAGTAATG
TCCCCAGCCCTTATATGACCCCTTCGATGTTGTGGAATTTATCGAGTCTTGTAAACGATATTCTAAAAGATGAAAATATT
GCTGGAGTGGTGATAACCCATGGAACGGATACCCTTGAAGAATCCGCTTATTTTCTGGATCTGACCGTTCAATCCGATAA
GCCGGTTGTTTGCACGGCAGCCATGCGAAACATCAACGAGCTAGGTACCGATGGCCCAAGGAACGTTGTATCTTCTGTGA
AGGTAGCTTTGTCCCCGGCTTCAAGAGGATTGGGGACATTGGTTTGTTTGAACGACGAGATTCATGCTGCAAAGGAAGTT
ACAAAAACCTATACCAGTAACGTGGCTACCTTTGATTCCCCTGGATATGGACCTCTCGGAATAGTTGATGAAGACAAGGT
GGTATACTTTAGAAGATTATACTATAGAGAAAACTACAGAGTTAGATTCATTGAAGAAAATGTAGCTCTGATAAAAACCT
TTGTAGGTGATGACGGAAGAATAATAGAAAAATTACCAGAACTTGGTTACAAAGGTCTCGTGATAGAAGCCTTTGGGCGC
GGAAACGTCCCGCCAAAGGTTGAAGATGCTTTAAAAGTACTGATAAAAGATCTTAGTTTTCCCGTTGTGATAACATCACG
ATGCTTCAAAGGAAGGGTTCTCGGCATTTATGGGTACAAAGGTGGCGGACGGAGTTTACATGATTTAGGCGTTATTTTTG
CACCGGGCCTTTCCTCTCAAAAAGCTCGAATTAAATTAATGGTAGTAATGGGGTTGACCGATGATCTAACGAAAATTGCT
GAATATTTTCAGCATAGTTGA

Upstream 100 bases:

>100_bases
ACAGACCCTTCAAAAATTGGGAAGAACTCGTGAATTTGCTTGAGCAGGAAACCGTAAACAATCTTAAAAAATTAAGACTT
CAGATAGGAGGTAAGACGGA

Downstream 100 bases:

>100_bases
AAGGAGAATGCTCGCGTATGAAGCGTTCCAACAAAGGTAGATTGTTTGTGGCGTTACTGACATTTTTGTATCTCTTCATC
GCGTTCATGATTTTCTTACT

Product: Asparaginase/glutaminase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MPEIAIITTGGTIAMVRDPSTGASVPASGMNVHVAEVDGLSAIAKIRHYDFSNVPSPYMTPSMLWNLSSLVNDILKDENI
AGVVITHGTDTLEESAYFLDLTVQSDKPVVCTAAMRNINELGTDGPRNVVSSVKVALSPASRGLGTLVCLNDEIHAAKEV
TKTYTSNVATFDSPGYGPLGIVDEDKVVYFRRLYYRENYRVRFIEENVALIKTFVGDDGRIIEKLPELGYKGLVIEAFGR
GNVPPKVEDALKVLIKDLSFPVVITSRCFKGRVLGIYGYKGGGRSLHDLGVIFAPGLSSQKARIKLMVVMGLTDDLTKIA
EYFQHS

Sequences:

>Translated_326_residues
MPEIAIITTGGTIAMVRDPSTGASVPASGMNVHVAEVDGLSAIAKIRHYDFSNVPSPYMTPSMLWNLSSLVNDILKDENI
AGVVITHGTDTLEESAYFLDLTVQSDKPVVCTAAMRNINELGTDGPRNVVSSVKVALSPASRGLGTLVCLNDEIHAAKEV
TKTYTSNVATFDSPGYGPLGIVDEDKVVYFRRLYYRENYRVRFIEENVALIKTFVGDDGRIIEKLPELGYKGLVIEAFGR
GNVPPKVEDALKVLIKDLSFPVVITSRCFKGRVLGIYGYKGGGRSLHDLGVIFAPGLSSQKARIKLMVVMGLTDDLTKIA
EYFQHS
>Mature_325_residues
PEIAIITTGGTIAMVRDPSTGASVPASGMNVHVAEVDGLSAIAKIRHYDFSNVPSPYMTPSMLWNLSSLVNDILKDENIA
GVVITHGTDTLEESAYFLDLTVQSDKPVVCTAAMRNINELGTDGPRNVVSSVKVALSPASRGLGTLVCLNDEIHAAKEVT
KTYTSNVATFDSPGYGPLGIVDEDKVVYFRRLYYRENYRVRFIEENVALIKTFVGDDGRIIEKLPELGYKGLVIEAFGRG
NVPPKVEDALKVLIKDLSFPVVITSRCFKGRVLGIYGYKGGGRSLHDLGVIFAPGLSSQKARIKLMVVMGLTDDLTKIAE
YFQHS

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=206, Percent_Identity=27.6699029126214, Blast_Score=66, Evalue=4e-11,
Organism=Escherichia coli, GI1789327, Length=341, Percent_Identity=34.0175953079179, Blast_Score=138, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17558278, Length=239, Percent_Identity=26.7782426778243, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17558280, Length=239, Percent_Identity=26.7782426778243, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI193207372, Length=239, Percent_Identity=26.7782426778243, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI193207374, Length=239, Percent_Identity=26.7782426778243, Blast_Score=78, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6323189, Length=284, Percent_Identity=30.9859154929577, Blast_Score=123, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6323187, Length=284, Percent_Identity=30.9859154929577, Blast_Score=123, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6323186, Length=284, Percent_Identity=30.9859154929577, Blast_Score=123, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6323184, Length=284, Percent_Identity=30.9859154929577, Blast_Score=123, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6320527, Length=340, Percent_Identity=29.4117647058824, Blast_Score=106, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24645530, Length=337, Percent_Identity=28.1899109792285, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24639609, Length=352, Percent_Identity=26.7045454545455, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 35448; Mature: 35317

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEIAIITTGGTIAMVRDPSTGASVPASGMNVHVAEVDGLSAIAKIRHYDFSNVPSPYMT
CCCEEEEECCCEEEEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCC
PSMLWNLSSLVNDILKDENIAGVVITHGTDTLEESAYFLDLTVQSDKPVVCTAAMRNINE
HHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEEECCCCCEEEHHHHHHHHH
LGTDGPRNVVSSVKVALSPASRGLGTLVCLNDEIHAAKEVTKTYTSNVATFDSPGYGPLG
HCCCCHHHHHHHHHEEECCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEECCCCCCCCCC
IVDEDKVVYFRRLYYRENYRVRFIEENVALIKTFVGDDGRIIEKLPELGYKGLVIEAFGR
CCCCCHHEEEEEHHHCCCCEEEEEECCEEEEEEEECCCCHHHHHHHHCCCCEEEEEECCC
GNVPPKVEDALKVLIKDLSFPVVITSRCFKGRVLGIYGYKGGGRSLHDLGVIFAPGLSSQ
CCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEEECCCCCCHHHCCEEEECCCCCC
KARIKLMVVMGLTDDLTKIAEYFQHS
CCEEEEEEEECCCHHHHHHHHHHCCC
>Mature Secondary Structure 
PEIAIITTGGTIAMVRDPSTGASVPASGMNVHVAEVDGLSAIAKIRHYDFSNVPSPYMT
CCEEEEECCCEEEEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCCCC
PSMLWNLSSLVNDILKDENIAGVVITHGTDTLEESAYFLDLTVQSDKPVVCTAAMRNINE
HHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEEECCCCCEEEHHHHHHHHH
LGTDGPRNVVSSVKVALSPASRGLGTLVCLNDEIHAAKEVTKTYTSNVATFDSPGYGPLG
HCCCCHHHHHHHHHEEECCCCCCCEEEEEECCCHHHHHHHHHHHHCCCEECCCCCCCCCC
IVDEDKVVYFRRLYYRENYRVRFIEENVALIKTFVGDDGRIIEKLPELGYKGLVIEAFGR
CCCCCHHEEEEEHHHCCCCEEEEEECCEEEEEEEECCCCHHHHHHHHCCCCEEEEEECCC
GNVPPKVEDALKVLIKDLSFPVVITSRCFKGRVLGIYGYKGGGRSLHDLGVIFAPGLSSQ
CCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEEECCCCCCHHHCCEEEECCCCCC
KARIKLMVVMGLTDDLTKIAEYFQHS
CCEEEEEEEECCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1916233 [H]