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The map label for this gene is amtB [C]

Identifier: 239616607

GI number: 239616607

Start: 224663

End: 225904

Strand: Reverse

Name: amtB [C]

Synonym: Kole_0200

Alternate gene names: 239616607

Gene position: 225904-224663 (Counterclockwise)

Preceding gene: 239616608

Following gene: 239616606

Centisome position: 9.81

GC content: 44.44

Gene sequence:

>1242_bases
TTGAAAAAATTAACGGAAGAGGTGAAGAAAGTGTTTGACACAGGGAATACGGGTTTCATGCTTGTTGCGACAAGCCTTGT
TATGTTGATGACACCCGGTCTGGCTTTCTTTTATGGTGGACTCGTGAGCAGAAAAAATGTGCTTACGATAATGATGCAGA
GTTTTGTATCAATGGGATGGACAACCATCTTGTGGGTTACCGTTGGATTTACGATGTGCTTTGGTGGAGATATCGGTGGA
ATAATTGGAGACGGGAAGTATTTATTTCTCCATGGTATTAGCCTCAGCACAGCTTTTGGTTCCAATATGGGAATTCCCAT
GCTGGTGTTTGTGGCGTACCAGATGATGTTTGCCATAATAACCCCTGCATTGATTACAGGTGCTTTCGCAGATAGGGTTC
GTTTCAAGGCGTATATGATATTCCTGACGGTTTGGCTTCTTTTCGTATATTTCCCGCTGGTGCATATGGTATGGGGTGGA
GGCCTCTTTCAGCAGTGGGGAGTTCTTGACTTTGCAGGAGGTATAGTCGTACACGCGAGCGCAGGTTTTGCAGCTCTTGC
ATCGGTATTCTTTGTAGGAAAGAGGAAAGTTAAAAACACGAGACCACATAGCATACCTTTGGTTGCTCTGGGCACGGCGT
TGCTATGGTTCGGATGGTATGGTTTTAATGCTGGAAGTGAATTGAGAGTGGACTCCGTAACAGTTTCAGCATTCTTAAAC
ACCGATGTTGCGGCATCCTTCGCGGCAATAACATGGTTGATTCTCGCGGTGATATTTGAGAAAAAGCCGAAAGTTTTGGG
GATGCTTACAGGTGCTGTCGCAGGTCTCGCTACAATAACCCCTGCTGCGGGATATGTAAACGTACAGTCATCGATGTTGA
TTGGAATAATTGCTTCTCTCATTGGGTATGCAGCCATCGCATACAAAAATAAAAAAGGATGGGATGATGCTCTGGATGTT
TGGGGAGTCCATGGAATGGGAGGATACGTTGGTATTTTATTGTTAGGTCTGTTTGCCAGCACCAACATTAATCCGAATGG
TGCGAATGGTCTTTTCTTTGGAGATGCGTCCTTCTTTCTCAAAGAATTTGTTGCTGTTACAGTAACCGCGGTTTATGCAT
TTGTGTTCACATATGTAGCCCTCTGGCTTATTAACAAGATCACCACAGTGCGGGTTTCAGAAGAGACAGAGACGAATGGT
CTAGACCTGTTTGAATTCGGTGAAGAGGCTTATGTTGAATAA

Upstream 100 bases:

>100_bases
GTGAGGGAGGTGAGAATGTTCCCGAGAAAAATCAATGTTAGTCATAAGAATATGTATTCAAGATTGAGTTCAAGCGTGTG
CGGGTTATACGGAGTAATGG

Downstream 100 bases:

>100_bases
ATTGATTTTTTAGTGCAAGTCATACGCGATAGAGTATATCTGGCGAGTCACTCATAGAGTGGTTCGCCAGATTTTTTATT
TTAGTTGCCGAAAAGTAGAG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MKKLTEEVKKVFDTGNTGFMLVATSLVMLMTPGLAFFYGGLVSRKNVLTIMMQSFVSMGWTTILWVTVGFTMCFGGDIGG
IIGDGKYLFLHGISLSTAFGSNMGIPMLVFVAYQMMFAIITPALITGAFADRVRFKAYMIFLTVWLLFVYFPLVHMVWGG
GLFQQWGVLDFAGGIVVHASAGFAALASVFFVGKRKVKNTRPHSIPLVALGTALLWFGWYGFNAGSELRVDSVTVSAFLN
TDVAASFAAITWLILAVIFEKKPKVLGMLTGAVAGLATITPAAGYVNVQSSMLIGIIASLIGYAAIAYKNKKGWDDALDV
WGVHGMGGYVGILLLGLFASTNINPNGANGLFFGDASFFLKEFVAVTVTAVYAFVFTYVALWLINKITTVRVSEETETNG
LDLFEFGEEAYVE

Sequences:

>Translated_413_residues
MKKLTEEVKKVFDTGNTGFMLVATSLVMLMTPGLAFFYGGLVSRKNVLTIMMQSFVSMGWTTILWVTVGFTMCFGGDIGG
IIGDGKYLFLHGISLSTAFGSNMGIPMLVFVAYQMMFAIITPALITGAFADRVRFKAYMIFLTVWLLFVYFPLVHMVWGG
GLFQQWGVLDFAGGIVVHASAGFAALASVFFVGKRKVKNTRPHSIPLVALGTALLWFGWYGFNAGSELRVDSVTVSAFLN
TDVAASFAAITWLILAVIFEKKPKVLGMLTGAVAGLATITPAAGYVNVQSSMLIGIIASLIGYAAIAYKNKKGWDDALDV
WGVHGMGGYVGILLLGLFASTNINPNGANGLFFGDASFFLKEFVAVTVTAVYAFVFTYVALWLINKITTVRVSEETETNG
LDLFEFGEEAYVE
>Mature_413_residues
MKKLTEEVKKVFDTGNTGFMLVATSLVMLMTPGLAFFYGGLVSRKNVLTIMMQSFVSMGWTTILWVTVGFTMCFGGDIGG
IIGDGKYLFLHGISLSTAFGSNMGIPMLVFVAYQMMFAIITPALITGAFADRVRFKAYMIFLTVWLLFVYFPLVHMVWGG
GLFQQWGVLDFAGGIVVHASAGFAALASVFFVGKRKVKNTRPHSIPLVALGTALLWFGWYGFNAGSELRVDSVTVSAFLN
TDVAASFAAITWLILAVIFEKKPKVLGMLTGAVAGLATITPAAGYVNVQSSMLIGIIASLIGYAAIAYKNKKGWDDALDV
WGVHGMGGYVGILLLGLFASTNINPNGANGLFFGDASFFLKEFVAVTVTAVYAFVFTYVALWLINKITTVRVSEETETNG
LDLFEFGEEAYVE

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=417, Percent_Identity=40.0479616306954, Blast_Score=289, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI17550182, Length=444, Percent_Identity=26.8018018018018, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17550180, Length=457, Percent_Identity=25.164113785558, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI133931100, Length=415, Percent_Identity=25.5421686746988, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI32564042, Length=429, Percent_Identity=25.1748251748252, Blast_Score=102, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6325396, Length=422, Percent_Identity=32.2274881516588, Blast_Score=205, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6324187, Length=430, Percent_Identity=31.1627906976744, Blast_Score=198, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6321559, Length=428, Percent_Identity=31.5420560747664, Blast_Score=196, Evalue=7e-51,
Organism=Drosophila melanogaster, GI161078314, Length=410, Percent_Identity=27.5609756097561, Blast_Score=104, Evalue=9e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 44673; Mature: 44673

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLTEEVKKVFDTGNTGFMLVATSLVMLMTPGLAFFYGGLVSRKNVLTIMMQSFVSMGW
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHCCH
TTILWVTVGFTMCFGGDIGGIIGDGKYLFLHGISLSTAFGSNMGIPMLVFVAYQMMFAII
HHHHHHHHHHHHHCCCCCCCEECCCCEEEEECCEEEHHCCCCCCHHHHHHHHHHHHHHHH
TPALITGAFADRVRFKAYMIFLTVWLLFVYFPLVHMVWGGGLFQQWGVLDFAGGIVVHAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEECCCEEEEEC
AGFAALASVFFVGKRKVKNTRPHSIPLVALGTALLWFGWYGFNAGSELRVDSVTVSAFLN
CCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEHEEEEHHHC
TDVAASFAAITWLILAVIFEKKPKVLGMLTGAVAGLATITPAAGYVNVQSSMLIGIIASL
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHH
IGYAAIAYKNKKGWDDALDVWGVHGMGGYVGILLLGLFASTNINPNGANGLFFGDASFFL
HHHHHHHCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHH
KEFVAVTVTAVYAFVFTYVALWLINKITTVRVSEETETNGLDLFEFGEEAYVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCHHHCCHHHCCC
>Mature Secondary Structure
MKKLTEEVKKVFDTGNTGFMLVATSLVMLMTPGLAFFYGGLVSRKNVLTIMMQSFVSMGW
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHCCH
TTILWVTVGFTMCFGGDIGGIIGDGKYLFLHGISLSTAFGSNMGIPMLVFVAYQMMFAII
HHHHHHHHHHHHHCCCCCCCEECCCCEEEEECCEEEHHCCCCCCHHHHHHHHHHHHHHHH
TPALITGAFADRVRFKAYMIFLTVWLLFVYFPLVHMVWGGGLFQQWGVLDFAGGIVVHAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEECCCEEEEEC
AGFAALASVFFVGKRKVKNTRPHSIPLVALGTALLWFGWYGFNAGSELRVDSVTVSAFLN
CCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEHEEEEHHHC
TDVAASFAAITWLILAVIFEKKPKVLGMLTGAVAGLATITPAAGYVNVQSSMLIGIIASL
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHH
IGYAAIAYKNKKGWDDALDVWGVHGMGGYVGILLLGLFASTNINPNGANGLFFGDASFFL
HHHHHHHCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECCHHHHH
KEFVAVTVTAVYAFVFTYVALWLINKITTVRVSEETETNGLDLFEFGEEAYVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]