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The map label for this gene is ykfC [H]

Identifier: 239616548

GI number: 239616548

Start: 157796

End: 159067

Strand: Reverse

Name: ykfC [H]

Synonym: Kole_0140

Alternate gene names: 239616548

Gene position: 159067-157796 (Counterclockwise)

Preceding gene: 239616549

Following gene: 239616547

Centisome position: 6.91

GC content: 41.98

Gene sequence:

>1272_bases
ATGAGGAAGTACTACAGTCTTATTGACAAAGTTTATCTGGAATCGAACCTGGCCAAAGCATATCACAAGGTGCGAAGAAA
CAACGGAGCACCCGGGATAGATGGAGTAACGGTTCAGGAGTATGGAGAAAACCTTCTGGAAAGGATTAAGAAACTCAGCG
AAAAGTTGCGAAAAGGAGAATACCGGCCTTCACCGGTAAAAAGGGTAGAAATCCCGAAAGGAAATGGGAAGACACGTATG
CTCGGGATACCGACGGTAGAGGATCGAATAGTACAACAATCGCTGAAAGAAATAATGGAACCAATCTTTGAGGAAGGATT
TCATCCTTCAAGTTATGGATACAGGAAAGGAAGGAATCCACACCAGGCAGTAGAGAAAGCCTATGCCTTCGCGTGCAAAT
ACAAAATGAAATACGTGGTACAGTTAGACCTTAGTCAATGCTTTGACACCTTAGACCACGAAAAGATGATAGACGCAGTA
GCGGAAAGAATAAGCGATGGCAAAATATTGAGATTAATAAGAAGTTTCCTCAAAAGTGGAGTCATAACGGACCAATACCA
ACCCAGTGAGATGGGAAGTCCACAGGGCGGAGTAATAAGCCCCTTGTTAGCCAACATCTATCTGAACAAATTTGACCAGA
AAATGATGGCCAGAGGAATAAGGATAGTGAGATATGCAGACGACATACTAATCTTCGCAAAGAGCTACAAAAGCGCGGAA
AAATACCTAAAAATAGCTATCCGGATACTGGAAAAAGAGCTGAAATTGAAGGTCAACAAAGAAAAGACGAGAATAACCAC
GATAGATGATGGAATCGAATTCCTTGGATTTACCATACAAAAAGGCAAAATACGAATCCAGGAAAAGAAAATAAAAAGGT
TCAAAGCGAAAGTAAAAACACTCACCCGGAGAAACCAATGTACACCGATTGGAGAGATAATAAAACGGCTAAACCAATTG
CTGAGAGGCTTCAGCAATTACTACAAAATAATCGATTGGGTGGGAGTATTCAAAGGCCTCATGGGTTGGATAAGAAGGAG
ATTGAGAGCCATAATCCTGCGACAATGGAAGACGACAAAGAAACTATGCAGGGTGATGAGACAAAAAGGATACAGAGGCG
AAATATCTGGAATAAGGATGAACAAATGGAGAAGCTCTCAATCCCGGTTGGTCAGTAGGCTACTGCCGAACAAGTATTTC
CAGAAGATAGGGCTGTATGATATGAAGCTTTCGCATGTACCACTGTCGGAGAATCCGATACTCAATCCATGA

Upstream 100 bases:

>100_bases
AGGAAGATTCCCGGGGGGGGTAAAGAATCCAATCTTTGGATGGGAGAAGAAACCGATTCTGTTATATGCGACTTATCCGG
AAGGCCATACAGGAGGAAAC

Downstream 100 bases:

>100_bases
GCCGTGTACGTTGGCCCGTACGCACGGTTCTGTGAGAGGACGGGGGGATTCCCCCCCTCCTACTCGATAACCGCAAGCGG
TTGAAAAAGGCTGTCATTCT

Product: RNA-directed DNA polymerase (Reverse transcriptase)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MRKYYSLIDKVYLESNLAKAYHKVRRNNGAPGIDGVTVQEYGENLLERIKKLSEKLRKGEYRPSPVKRVEIPKGNGKTRM
LGIPTVEDRIVQQSLKEIMEPIFEEGFHPSSYGYRKGRNPHQAVEKAYAFACKYKMKYVVQLDLSQCFDTLDHEKMIDAV
AERISDGKILRLIRSFLKSGVITDQYQPSEMGSPQGGVISPLLANIYLNKFDQKMMARGIRIVRYADDILIFAKSYKSAE
KYLKIAIRILEKELKLKVNKEKTRITTIDDGIEFLGFTIQKGKIRIQEKKIKRFKAKVKTLTRRNQCTPIGEIIKRLNQL
LRGFSNYYKIIDWVGVFKGLMGWIRRRLRAIILRQWKTTKKLCRVMRQKGYRGEISGIRMNKWRSSQSRLVSRLLPNKYF
QKIGLYDMKLSHVPLSENPILNP

Sequences:

>Translated_423_residues
MRKYYSLIDKVYLESNLAKAYHKVRRNNGAPGIDGVTVQEYGENLLERIKKLSEKLRKGEYRPSPVKRVEIPKGNGKTRM
LGIPTVEDRIVQQSLKEIMEPIFEEGFHPSSYGYRKGRNPHQAVEKAYAFACKYKMKYVVQLDLSQCFDTLDHEKMIDAV
AERISDGKILRLIRSFLKSGVITDQYQPSEMGSPQGGVISPLLANIYLNKFDQKMMARGIRIVRYADDILIFAKSYKSAE
KYLKIAIRILEKELKLKVNKEKTRITTIDDGIEFLGFTIQKGKIRIQEKKIKRFKAKVKTLTRRNQCTPIGEIIKRLNQL
LRGFSNYYKIIDWVGVFKGLMGWIRRRLRAIILRQWKTTKKLCRVMRQKGYRGEISGIRMNKWRSSQSRLVSRLLPNKYF
QKIGLYDMKLSHVPLSENPILNP
>Mature_423_residues
MRKYYSLIDKVYLESNLAKAYHKVRRNNGAPGIDGVTVQEYGENLLERIKKLSEKLRKGEYRPSPVKRVEIPKGNGKTRM
LGIPTVEDRIVQQSLKEIMEPIFEEGFHPSSYGYRKGRNPHQAVEKAYAFACKYKMKYVVQLDLSQCFDTLDHEKMIDAV
AERISDGKILRLIRSFLKSGVITDQYQPSEMGSPQGGVISPLLANIYLNKFDQKMMARGIRIVRYADDILIFAKSYKSAE
KYLKIAIRILEKELKLKVNKEKTRITTIDDGIEFLGFTIQKGKIRIQEKKIKRFKAKVKTLTRRNQCTPIGEIIKRLNQL
LRGFSNYYKIIDWVGVFKGLMGWIRRRLRAIILRQWKTTKKLCRVMRQKGYRGEISGIRMNKWRSSQSRLVSRLLPNKYF
QKIGLYDMKLSHVPLSENPILNP

Specific function: Unknown

COG id: COG3344

COG function: function code L; Retron-type reverse transcriptase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 reverse transcriptase domain [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6226521, Length=373, Percent_Identity=27.8820375335121, Blast_Score=133, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6226520, Length=353, Percent_Identity=27.4787535410765, Blast_Score=117, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015706
- InterPro:   IPR000477 [H]

Pfam domain/function: PF00078 RVT_1 [H]

EC number: NA

Molecular weight: Translated: 49321; Mature: 49321

Theoretical pI: Translated: 10.74; Mature: 10.74

Prosite motif: PS50878 RT_POL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKYYSLIDKVYLESNLAKAYHKVRRNNGAPGIDGVTVQEYGENLLERIKKLSEKLRKGE
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
YRPSPVKRVEIPKGNGKTRMLGIPTVEDRIVQQSLKEIMEPIFEEGFHPSSYGYRKGRNP
CCCCCCCEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
HQAVEKAYAFACKYKMKYVVQLDLSQCFDTLDHEKMIDAVAERISDGKILRLIRSFLKSG
HHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
VITDQYQPSEMGSPQGGVISPLLANIYLNKFDQKMMARGIRIVRYADDILIFAKSYKSAE
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHEEHHHHHHHHHHHHHHH
KYLKIAIRILEKELKLKVNKEKTRITTIDDGIEFLGFTIQKGKIRIQEKKIKRFKAKVKT
HHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHEEECCCEEHHHHHHHHHHHHHHH
LTRRNQCTPIGEIIKRLNQLLRGFSNYYKIIDWVGVFKGLMGWIRRRLRAIILRQWKTTK
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLCRVMRQKGYRGEISGIRMNKWRSSQSRLVSRLLPNKYFQKIGLYDMKLSHVPLSENPI
HHHHHHHHCCCCCCCCCEEHHHHCCHHHHHHHHHHHHHHHHHHCCHHEEECCCCCCCCCC
LNP
CCC
>Mature Secondary Structure
MRKYYSLIDKVYLESNLAKAYHKVRRNNGAPGIDGVTVQEYGENLLERIKKLSEKLRKGE
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
YRPSPVKRVEIPKGNGKTRMLGIPTVEDRIVQQSLKEIMEPIFEEGFHPSSYGYRKGRNP
CCCCCCCEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCH
HQAVEKAYAFACKYKMKYVVQLDLSQCFDTLDHEKMIDAVAERISDGKILRLIRSFLKSG
HHHHHHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
VITDQYQPSEMGSPQGGVISPLLANIYLNKFDQKMMARGIRIVRYADDILIFAKSYKSAE
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHEEHHHHHHHHHHHHHHH
KYLKIAIRILEKELKLKVNKEKTRITTIDDGIEFLGFTIQKGKIRIQEKKIKRFKAKVKT
HHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHEEECCCEEHHHHHHHHHHHHHHH
LTRRNQCTPIGEIIKRLNQLLRGFSNYYKIIDWVGVFKGLMGWIRRRLRAIILRQWKTTK
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLCRVMRQKGYRGEISGIRMNKWRSSQSRLVSRLLPNKYFQKIGLYDMKLSHVPLSENPI
HHHHHHHHCCCCCCCCCEEHHHHCCHHHHHHHHHHHHHHHHHHCCHHEEECCCCCCCCCC
LNP
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]