Click here to switch to the map view.

The map label for this gene is uvrB

Identifier: 238925135

GI number: 238925135

Start: 2666796

End: 2668778

Strand: Reverse

Name: uvrB

Synonym: EUBREC_2788

Alternate gene names: 238925135

Gene position: 2668778-2666796 (Counterclockwise)

Preceding gene: 238925136

Following gene: 238925134

Centisome position: 77.36

GC content: 47.25

Gene sequence:

>1983_bases
ATGGAATTTAAACTGCATTCAAAATACAAGCCGACCGGCGACCAGCCACAGGCGATAGAGCAGCTTGTGAAGGGCTTTAA
GGAGGGCAACCAGTTTGAAACCTTACTTGGAGTTACAGGCTCCGGCAAGACCTTTACCATGGCCAATGTCATTGCAGCAC
TCAATAAGCCTACACTTATCATATCGCACAATAAGACACTGGCGGGGCAGCTCTATGGTGAGATGAAGGAGTTTTTCCCT
GAGAATGCAGTAGAGTATTTTGTTTCCTACTACGACTATTACCAGCCTGAGGCATATGTGCCGCAGTCTGATACGTACAT
AGCCAAGGATTCCTCTGTAAATGATGAGATAGATAAGCTCAGGCTTTCAGCCACGGCAGCACTCACAGAGCGAAAGGATG
TCATTGTCGTGGCATCGGTTTCCTGCATATACGGACTTGGAAGTCCTGATGAGTGGACAGGCATGAGTATTTCGCTGCGC
CCGGGGCAAGAGAGGGACAGGGATGATCTCGCGAGGGCTCTCATAGATTTACAGTACACCAGGAATGATATGGATTTTCA
CCGAGGCACCTTCAGAGTGCATGGTGATGTGCTTGATATATTTCCGGCAAATGCCGATGATACAGCAATCAGGGTTGAGT
TTTTCGGTGATGAGATAGATCGGATAGTAGAGGTTGATGTGCTGACCGGCGAGGTAAAGTGCTCGCTTGAGCACACGATG
ATTTTCCCTGCATCCCACTATGTAGTTTCGCAGGAGAAGATAAATGAGGCCTGCCTCAATATAGAGAAAGAGCTTGATGA
GCGTGTGAAGTATTTTAAAGGCGAGGACAAGCTCATAGAGGCTCAGCGAATAGCAGAGAGGACTAATTTTGACATAGAGA
TGATGAGGGAGACAGGCTTCTGCTCAGGAATTGAGAACTATACGAGGCATCTGAACTTTGCAAAGCCGGGGGAGCCTCCT
ATGACACTCATAGATTTCTTCCCGGATGATTTTCTCATAATCGTGGATGAGTCACATATCACGATTCCACAGATAGGCGG
TATGTATGCGGGTGACCGCTCTAGAAAGACCACTCTTGTTGATTATGGCTTCAGACTGCCCTCGGCCCTTGACAACAGAC
CTCTCAATTTTGAGGAGTTCGAGGGTAAAATAGACCAGATGCTCTTTGTCTCAGCCACTCCTAATAAGTATGAGAATGAG
CATGAGCTGCTTCGTGCGGAGCAGATTATCAGACCGACGGGACTTTTGGATCCTGAGATATCAGTGCGCCCTGTTGAGGG
ACAGATTGATGACCTTGTGTCAGAGGTCAATAAGGAGACTGCAAACCATCACAAGGTGCTTATCACCACACTCACAAAGC
GTATGGCTGAGGATCTCACGAACTATATGGCAGAGCTTGGCATCAGGGTAAAGTACCTGCATTCAGATATAGATACACTT
GAGAGAGCGGAGATAATACGTGACCTGCGTCTTGACGTATTTGACGTGCTCGTCGGCATCAATCTGCTCAGGGAGGGTCT
TGATATCCCGGAGATTACACTTGTTGCCATACTCGATGCGGATAAGGAAGGTTTCCTTCGCTCAGAGACCTCACTTATAC
AGACGATAGGACGTGCCGCACGTAACAGTGAGGGCCATGTTATCATGTACGCTGACAAGATTACAGACTCCATGCGTGTC
GCAATTGACGAGACAAAGCGTCGTAGAAAGGTACAGGAAGAGTACAATGAGGCTCACGGCATCACGCCACAGACTATACA
GAAATCCGTGCGTGATCTCATAGCTATCTCAAAGAAGGTAGCGGCCGATGAGAATGCTCTTGACAAAGATCCGGAGTCAA
TGAGCAAAAAAGAGCTTGAGAAGCATATTGCAGATATAGAGAAAAAGATGAAAAAAGCAGCGGCAGAGCTCAATTTCGAG
GCTGCTGCAGAGTACAGGGATAAGCTTATTATGCTGAAAAACACACTGAGAGATATACGATAA

Upstream 100 bases:

>100_bases
GTAAAATAAAGCACGTAAGTGCATACTATAAATGATTGACTTTGTCTTTTTATAATTTTACAAAAGCAGTATTTAAGATT
TTTTGGAGGAAGCGTGCGTT

Downstream 100 bases:

>100_bases
AAGCTTATGATATTTGGAAGCAATTTGGAGGAACACAAATTATGGCAAAAAAAGAAAGAAAATACATCAAAATACGAGGT
GCCAGCGAACACAATCTAAA

Product: excinuclease ABC subunit B

Products: NA

Alternate protein names: Protein uvrB; Excinuclease ABC subunit B

Number of amino acids: Translated: 660; Mature: 660

Protein sequence:

>660_residues
MEFKLHSKYKPTGDQPQAIEQLVKGFKEGNQFETLLGVTGSGKTFTMANVIAALNKPTLIISHNKTLAGQLYGEMKEFFP
ENAVEYFVSYYDYYQPEAYVPQSDTYIAKDSSVNDEIDKLRLSATAALTERKDVIVVASVSCIYGLGSPDEWTGMSISLR
PGQERDRDDLARALIDLQYTRNDMDFHRGTFRVHGDVLDIFPANADDTAIRVEFFGDEIDRIVEVDVLTGEVKCSLEHTM
IFPASHYVVSQEKINEACLNIEKELDERVKYFKGEDKLIEAQRIAERTNFDIEMMRETGFCSGIENYTRHLNFAKPGEPP
MTLIDFFPDDFLIIVDESHITIPQIGGMYAGDRSRKTTLVDYGFRLPSALDNRPLNFEEFEGKIDQMLFVSATPNKYENE
HELLRAEQIIRPTGLLDPEISVRPVEGQIDDLVSEVNKETANHHKVLITTLTKRMAEDLTNYMAELGIRVKYLHSDIDTL
ERAEIIRDLRLDVFDVLVGINLLREGLDIPEITLVAILDADKEGFLRSETSLIQTIGRAARNSEGHVIMYADKITDSMRV
AIDETKRRRKVQEEYNEAHGITPQTIQKSVRDLIAISKKVAADENALDKDPESMSKKELEKHIADIEKKMKKAAAELNFE
AAAEYRDKLIMLKNTLRDIR

Sequences:

>Translated_660_residues
MEFKLHSKYKPTGDQPQAIEQLVKGFKEGNQFETLLGVTGSGKTFTMANVIAALNKPTLIISHNKTLAGQLYGEMKEFFP
ENAVEYFVSYYDYYQPEAYVPQSDTYIAKDSSVNDEIDKLRLSATAALTERKDVIVVASVSCIYGLGSPDEWTGMSISLR
PGQERDRDDLARALIDLQYTRNDMDFHRGTFRVHGDVLDIFPANADDTAIRVEFFGDEIDRIVEVDVLTGEVKCSLEHTM
IFPASHYVVSQEKINEACLNIEKELDERVKYFKGEDKLIEAQRIAERTNFDIEMMRETGFCSGIENYTRHLNFAKPGEPP
MTLIDFFPDDFLIIVDESHITIPQIGGMYAGDRSRKTTLVDYGFRLPSALDNRPLNFEEFEGKIDQMLFVSATPNKYENE
HELLRAEQIIRPTGLLDPEISVRPVEGQIDDLVSEVNKETANHHKVLITTLTKRMAEDLTNYMAELGIRVKYLHSDIDTL
ERAEIIRDLRLDVFDVLVGINLLREGLDIPEITLVAILDADKEGFLRSETSLIQTIGRAARNSEGHVIMYADKITDSMRV
AIDETKRRRKVQEEYNEAHGITPQTIQKSVRDLIAISKKVAADENALDKDPESMSKKELEKHIADIEKKMKKAAAELNFE
AAAEYRDKLIMLKNTLRDIR
>Mature_660_residues
MEFKLHSKYKPTGDQPQAIEQLVKGFKEGNQFETLLGVTGSGKTFTMANVIAALNKPTLIISHNKTLAGQLYGEMKEFFP
ENAVEYFVSYYDYYQPEAYVPQSDTYIAKDSSVNDEIDKLRLSATAALTERKDVIVVASVSCIYGLGSPDEWTGMSISLR
PGQERDRDDLARALIDLQYTRNDMDFHRGTFRVHGDVLDIFPANADDTAIRVEFFGDEIDRIVEVDVLTGEVKCSLEHTM
IFPASHYVVSQEKINEACLNIEKELDERVKYFKGEDKLIEAQRIAERTNFDIEMMRETGFCSGIENYTRHLNFAKPGEPP
MTLIDFFPDDFLIIVDESHITIPQIGGMYAGDRSRKTTLVDYGFRLPSALDNRPLNFEEFEGKIDQMLFVSATPNKYENE
HELLRAEQIIRPTGLLDPEISVRPVEGQIDDLVSEVNKETANHHKVLITTLTKRMAEDLTNYMAELGIRVKYLHSDIDTL
ERAEIIRDLRLDVFDVLVGINLLREGLDIPEITLVAILDADKEGFLRSETSLIQTIGRAARNSEGHVIMYADKITDSMRV
AIDETKRRRKVQEEYNEAHGITPQTIQKSVRDLIAISKKVAADENALDKDPESMSKKELEKHIADIEKKMKKAAAELNFE
AAAEYRDKLIMLKNTLRDIR

Specific function: The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. Upon binding of the uvrA(2)B(2) complex to a putative damaged site, the DNA

COG id: COG0556

COG function: function code L; Helicase subunit of the DNA excision repair complex

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 UVR domain

Homologues:

Organism=Escherichia coli, GI1786996, Length=662, Percent_Identity=59.6676737160121, Blast_Score=781, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UVRB_EUBR3 (C4ZHN1)

Other databases:

- EMBL:   CP001107
- RefSeq:   YP_002938652.1
- GeneID:   7964809
- GenomeReviews:   CP001107_GR
- KEGG:   ere:EUBREC_2788
- ProtClustDB:   PRK05298
- GO:   GO:0005737
- HAMAP:   MF_00204
- InterPro:   IPR014001
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR006935
- InterPro:   IPR001943
- InterPro:   IPR004807
- InterPro:   IPR009055
- SMART:   SM00487
- SMART:   SM00490
- TIGRFAMs:   TIGR00631

Pfam domain/function: PF00271 Helicase_C; PF04851 ResIII; PF02151 UVR; SSF46600 UvrB_C

EC number: NA

Molecular weight: Translated: 75061; Mature: 75061

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS51192 HELICASE_ATP_BIND_1; PS51194 HELICASE_CTER; PS50151 UVR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFKLHSKYKPTGDQPQAIEQLVKGFKEGNQFETLLGVTGSGKTFTMANVIAALNKPTLI
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCEEHHHHHHHHCCCCEEE
ISHNKTLAGQLYGEMKEFFPENAVEYFVSYYDYYQPEAYVPQSDTYIAKDSSVNDEIDKL
EECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCHHHHHH
RLSATAALTERKDVIVVASVSCIYGLGSPDEWTGMSISLRPGQERDRDDLARALIDLQYT
HHHHHHHHHCCCCEEEEEEEHHHCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHC
RNDMDFHRGTFRVHGDVLDIFPANADDTAIRVEFFGDEIDRIVEVDVLTGEVKCSLEHTM
CCCCCCCCCEEEEECCEEEEECCCCCCCEEEEEECCCCHHHEEEEEEEECEEEEEEEEEE
IFPASHYVVSQEKINEACLNIEKELDERVKYFKGEDKLIEAQRIAERTNFDIEMMRETGF
EECCHHHEECHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEHHHCCC
CSGIENYTRHLNFAKPGEPPMTLIDFFPDDFLIIVDESHITIPQIGGMYAGDRSRKTTLV
CCCHHHHHHHCCCCCCCCCCEEEEEECCCCEEEEEECCCEECCCCCCCCCCCCCCCEEEE
DYGFRLPSALDNRPLNFEEFEGKIDQMLFVSATPNKYENEHELLRAEQIIRPTGLLDPEI
ECCCCCCHHHCCCCCCHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCC
SVRPVEGQIDDLVSEVNKETANHHKVLITTLTKRMAEDLTNYMAELGIRVKYLHSDIDTL
EEEECCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHH
ERAEIIRDLRLDVFDVLVGINLLREGLDIPEITLVAILDADKEGFLRSETSLIQTIGRAA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHH
RNSEGHVIMYADKITDSMRVAIDETKRRRKVQEEYNEAHGITPQTIQKSVRDLIAISKKV
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
AADENALDKDPESMSKKELEKHIADIEKKMKKAAAELNFEAAAEYRDKLIMLKNTLRDIR
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEFKLHSKYKPTGDQPQAIEQLVKGFKEGNQFETLLGVTGSGKTFTMANVIAALNKPTLI
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCEEHHHHHHHHCCCCEEE
ISHNKTLAGQLYGEMKEFFPENAVEYFVSYYDYYQPEAYVPQSDTYIAKDSSVNDEIDKL
EECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCCHHHHHH
RLSATAALTERKDVIVVASVSCIYGLGSPDEWTGMSISLRPGQERDRDDLARALIDLQYT
HHHHHHHHHCCCCEEEEEEEHHHCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHC
RNDMDFHRGTFRVHGDVLDIFPANADDTAIRVEFFGDEIDRIVEVDVLTGEVKCSLEHTM
CCCCCCCCCEEEEECCEEEEECCCCCCCEEEEEECCCCHHHEEEEEEEECEEEEEEEEEE
IFPASHYVVSQEKINEACLNIEKELDERVKYFKGEDKLIEAQRIAERTNFDIEMMRETGF
EECCHHHEECHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEHHHCCC
CSGIENYTRHLNFAKPGEPPMTLIDFFPDDFLIIVDESHITIPQIGGMYAGDRSRKTTLV
CCCHHHHHHHCCCCCCCCCCEEEEEECCCCEEEEEECCCEECCCCCCCCCCCCCCCEEEE
DYGFRLPSALDNRPLNFEEFEGKIDQMLFVSATPNKYENEHELLRAEQIIRPTGLLDPEI
ECCCCCCHHHCCCCCCHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCC
SVRPVEGQIDDLVSEVNKETANHHKVLITTLTKRMAEDLTNYMAELGIRVKYLHSDIDTL
EEEECCCHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHH
ERAEIIRDLRLDVFDVLVGINLLREGLDIPEITLVAILDADKEGFLRSETSLIQTIGRAA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHH
RNSEGHVIMYADKITDSMRVAIDETKRRRKVQEEYNEAHGITPQTIQKSVRDLIAISKKV
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
AADENALDKDPESMSKKELEKHIADIEKKMKKAAAELNFEAAAEYRDKLIMLKNTLRDIR
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA