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The map label for this gene is thiC [H]

Identifier: 238924876

GI number: 238924876

Start: 2400148

End: 2401458

Strand: Reverse

Name: thiC [H]

Synonym: EUBREC_2527

Alternate gene names: 238924876

Gene position: 2401458-2400148 (Counterclockwise)

Preceding gene: 238924877

Following gene: 238924875

Centisome position: 69.61

GC content: 47.75

Gene sequence:

>1311_bases
ATGAGTAATTACACAACACAGATGGATGCCGCAAGAAAGGGCATCGTAACACCTGAAATTGAAAAGGTAGCAAAGAAAGA
GAAGATGGATGTAGACAAGCTGATGGAGCTTGTTGCAAGCGGAAAGGTAGCTATTCCTGCTAACAAGCATCACAAGTCAC
TTGATGCAGAGGGCGTTGGAAGCATGCTTCGCACAAAAATCAATGTAAACCTCGGTGTGTCAAGAGACTGCAAGGACTAT
GATGTGGAGATGCAGAAGGTCATGAGCGCTGTAAAGCTTGGAGCAGAGGCTATCATGGATCTTTCAAGCCATGGCAACAC
ACAGCCATTCAGACAGAAGCTTACAAGCGAATGTCCTGCAATGATAGGAACAGTGCCTGTTTATGACAGTGTTATCCACT
ATCAGCGTGATCTTGCAACACTTACAGCACAGGATTTTGTCGATGTAGTCAGACTCCATGCAGAGGATGGAGTGGACTTT
GTCACACTTCACTGTGGAATCACAAGAAAAACGATAGACCAGATTAAAAAGCACAAGCGTAAGATGAATATCGTAAGCCG
TGGAGGCAGCCTTGTATTTGCATGGATGTGCATGACAGGAGAGGAAAATCCTTTCTACGAGCACTATGATGAGATACTTG
AAATCTGTGAGGAGCACGATGTGACAATCTCGCTTGGAGATGCCTGCAGACCGGGATGCCTGGCAGATGCAACAGATGTA
TGCCAGATAGAGGAGCTTGTAAGACTTGGAGAGCTCACAAAGCGTGCATGGGATCACAACGTACAGGTCATGGTAGAGGG
ACCGGGACATGTGCCGCTTGATCAGGTAGCAGCAAACATGAAGGTACAGCAGTCAATCTGTATGGGAGCACCATTCTATG
TACTTGGACCTCTCGTAACAGATATCGCACCGGGCTATGACCACATCACAGCAGCAATCGGTGGAGCAGTGGCAGCAATG
AACGGCGCAGCTTTCCTTTGTTACGTGACACCTGCGGAGCATCTTGCTCTTCCAAATGTGGAGGATACAAAGCAGGGAAT
TATTGCGTCAAAGATTGCGGCTCATGCAGCTGATATTGCGAAGGGAATACCGGGAGCCAGAGATATAGATGATAAGATGG
CAGATGCCAGACGTGTGCTCGACTGGGATGCACAGTTTGCATGTGCACTTGATCCGGAGACAGCTAAGGCTATCAGGGAT
GACCGTCTGCCTGAGGATGACCACAGCGAGGCATGCAGTATGTGCGGTAAGTTCTGTGCAGTGCGAAGCATGAACAAGGC
ACTTGCAGGAGAGTATATTGATATACTATAA

Upstream 100 bases:

>100_bases
TACAATGAAATAAGCACATGTTAAATAATTAATAGCAGTAATAATAAGAATAGTAATAACTACTAATTAACAGTAATAAA
ATAAAGAACAGGAGAGTACA

Downstream 100 bases:

>100_bases
CTATTCAGTACAGAAACGACTCGCAACTGTGAAGGTACTGAACAGTTACGATATACTTTAAGGTGAGGTAATAATGGAAA
GTAAAAAGAACACTAAAAAA

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MSNYTTQMDAARKGIVTPEIEKVAKKEKMDVDKLMELVASGKVAIPANKHHKSLDAEGVGSMLRTKINVNLGVSRDCKDY
DVEMQKVMSAVKLGAEAIMDLSSHGNTQPFRQKLTSECPAMIGTVPVYDSVIHYQRDLATLTAQDFVDVVRLHAEDGVDF
VTLHCGITRKTIDQIKKHKRKMNIVSRGGSLVFAWMCMTGEENPFYEHYDEILEICEEHDVTISLGDACRPGCLADATDV
CQIEELVRLGELTKRAWDHNVQVMVEGPGHVPLDQVAANMKVQQSICMGAPFYVLGPLVTDIAPGYDHITAAIGGAVAAM
NGAAFLCYVTPAEHLALPNVEDTKQGIIASKIAAHAADIAKGIPGARDIDDKMADARRVLDWDAQFACALDPETAKAIRD
DRLPEDDHSEACSMCGKFCAVRSMNKALAGEYIDIL

Sequences:

>Translated_436_residues
MSNYTTQMDAARKGIVTPEIEKVAKKEKMDVDKLMELVASGKVAIPANKHHKSLDAEGVGSMLRTKINVNLGVSRDCKDY
DVEMQKVMSAVKLGAEAIMDLSSHGNTQPFRQKLTSECPAMIGTVPVYDSVIHYQRDLATLTAQDFVDVVRLHAEDGVDF
VTLHCGITRKTIDQIKKHKRKMNIVSRGGSLVFAWMCMTGEENPFYEHYDEILEICEEHDVTISLGDACRPGCLADATDV
CQIEELVRLGELTKRAWDHNVQVMVEGPGHVPLDQVAANMKVQQSICMGAPFYVLGPLVTDIAPGYDHITAAIGGAVAAM
NGAAFLCYVTPAEHLALPNVEDTKQGIIASKIAAHAADIAKGIPGARDIDDKMADARRVLDWDAQFACALDPETAKAIRD
DRLPEDDHSEACSMCGKFCAVRSMNKALAGEYIDIL
>Mature_435_residues
SNYTTQMDAARKGIVTPEIEKVAKKEKMDVDKLMELVASGKVAIPANKHHKSLDAEGVGSMLRTKINVNLGVSRDCKDYD
VEMQKVMSAVKLGAEAIMDLSSHGNTQPFRQKLTSECPAMIGTVPVYDSVIHYQRDLATLTAQDFVDVVRLHAEDGVDFV
TLHCGITRKTIDQIKKHKRKMNIVSRGGSLVFAWMCMTGEENPFYEHYDEILEICEEHDVTISLGDACRPGCLADATDVC
QIEELVRLGELTKRAWDHNVQVMVEGPGHVPLDQVAANMKVQQSICMGAPFYVLGPLVTDIAPGYDHITAAIGGAVAAMN
GAAFLCYVTPAEHLALPNVEDTKQGIIASKIAAHAADIAKGIPGARDIDDKMADARRVLDWDAQFACALDPETAKAIRDD
RLPEDDHSEACSMCGKFCAVRSMNKALAGEYIDIL

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=447, Percent_Identity=42.9530201342282, Blast_Score=365, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 47602; Mature: 47471

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNYTTQMDAARKGIVTPEIEKVAKKEKMDVDKLMELVASGKVAIPANKHHKSLDAEGVG
CCCCCCHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCEEECCCCCCCCCCHHHHH
SMLRTKINVNLGVSRDCKDYDVEMQKVMSAVKLGAEAIMDLSSHGNTQPFRQKLTSECPA
HHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
MIGTVPVYDSVIHYQRDLATLTAQDFVDVVRLHAEDGVDFVTLHCGITRKTIDQIKKHKR
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHH
KMNIVSRGGSLVFAWMCMTGEENPFYEHYDEILEICEEHDVTISLGDACRPGCLADATDV
HHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHH
CQIEELVRLGELTKRAWDHNVQVMVEGPGHVPLDQVAANMKVQQSICMGAPFYVLGPLVT
HHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHH
DIAPGYDHITAAIGGAVAAMNGAAFLCYVTPAEHLALPNVEDTKQGIIASKIAAHAADIA
HCCCCHHHHHHHHHHHHHHCCCCEEEEEECCHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
KGIPGARDIDDKMADARRVLDWDAQFACALDPETAKAIRDDRLPEDDHSEACSMCGKFCA
HCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHCCCCCCCHHHHHHHHHHHHH
VRSMNKALAGEYIDIL
HHHHHHHHHCHHHHCC
>Mature Secondary Structure 
SNYTTQMDAARKGIVTPEIEKVAKKEKMDVDKLMELVASGKVAIPANKHHKSLDAEGVG
CCCCCHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCEEECCCCCCCCCCHHHHH
SMLRTKINVNLGVSRDCKDYDVEMQKVMSAVKLGAEAIMDLSSHGNTQPFRQKLTSECPA
HHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
MIGTVPVYDSVIHYQRDLATLTAQDFVDVVRLHAEDGVDFVTLHCGITRKTIDQIKKHKR
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHH
KMNIVSRGGSLVFAWMCMTGEENPFYEHYDEILEICEEHDVTISLGDACRPGCLADATDV
HHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHH
CQIEELVRLGELTKRAWDHNVQVMVEGPGHVPLDQVAANMKVQQSICMGAPFYVLGPLVT
HHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHH
DIAPGYDHITAAIGGAVAAMNGAAFLCYVTPAEHLALPNVEDTKQGIIASKIAAHAADIA
HCCCCHHHHHHHHHHHHHHCCCCEEEEEECCHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
KGIPGARDIDDKMADARRVLDWDAQFACALDPETAKAIRDDRLPEDDHSEACSMCGKFCA
HCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHCCCCCCCHHHHHHHHHHHHH
VRSMNKALAGEYIDIL
HHHHHHHHHCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA