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The map label for this gene is pycB [H]

Identifier: 238923992

GI number: 238923992

Start: 1520124

End: 1521542

Strand: Direct

Name: pycB [H]

Synonym: EUBREC_1630

Alternate gene names: 238923992

Gene position: 1520124-1521542 (Clockwise)

Preceding gene: 238923991

Following gene: 238923993

Centisome position: 44.07

GC content: 43.83

Gene sequence:

>1419_bases
ATGTCAGAAGTGGTAAAGAAACCATTAAAAATAACAGAAACTGTTCTTCGTGATGCACATCAGTCACTGATTGCAACCAG
AATGACAACAGAGCAGATGCTTCCAATCGTTGATAAAATGGACAAGGTTGGATACAACGCAGTAGAGTGCTGGGGTGGTG
CAACATTTGATGCCTGCCTTAGATTCTTAAAGGAAGATCCATGGGATCGTCTCAGAAAATTAAGAGACGGCTTCAAAAAT
ACAAAGCTCCAGATGCTTTTCCGTGGACAGAATATCTTAGGATATCGTCCATATGCAGATGATGTTGTTGAGTATTTCGT
ACAGAAGTCAATCGCAAACGGAATCGATATCATCCGTATTTTCGACTGCTTAAATGATATCAGAAACTTACAGACAGCAG
TTACAGCATGTAACAAGGAAAAGGGACATGCCCAGGTAGCTCTTTCATACACACTTGGAGATGCTTACACACTTGACTAC
TGGATGGATATGGCAAAGAGAGTTGAGGACATGGGTGCTGACTCACTTTGTATCAAGGATATGGCCGGTCTTTTAGTTCC
TGCAAAGGCTACAGAGCTTGTTCAGGCTTTAAAGGATTCTACAAGCCTTCCTGTCGAGCTTCATACACACTACACATCAG
GTGTTGCTTCAATGACATACATGAAGGCTGTTGAGGCCGGAGTTGATATCATTGACACAGCTATGTCACCATTCGCTCTT
GGAACAAGCCAGCCTGCAACAGAGGTTATGGTTGAGGCATTCAAGGGTACAGAGTTTGATACAGGTCTTGATCTTAATCT
CTTAAGTGAGATTGCTGATTACTTCAGACCAATGAGAGAGGAAGCTTTAGCTTCAGGTCTCATGAACCCAAAGGTACTCG
GAGTAAATATCAATACTCTCCGTTATCAGGTTCCTGGAGGAATGCTTTCAAACCTTATCTCACAGCTTAAAGAGCAGGGC
AAAGAGGACAAGTACGAGGAGGTTCTCGCAGAGGTACCTCGTGTGCGTAAAGATCTCGGTGAGCCACCACTTGTAACTCC
TTCTTCACAGATCGTTGGTACACAGGCTGTATTTAACGTACTCATGGGCGAGAGATACAAGGTTGCTACTAAGGAGACTA
AGGATATCCTTCTTGGTAAGTATGGTCAGACAGTTAAGCCTTTCAACCCTGAGGTTGTTGACAAGGTTCTTGGTGATGAT
AAGAAGAATGCCATCACATGTCGTCCGGCAGATCTTCTTGAGCCAGAGCTTGACAAGATTGAGGCTGAGATGAAGCAGTG
GAAACAGCAGGATGAGGATGTTCTTTCTTATGCTCTTTTCCCACAGGTTGCTACTGATTTCTTCAAGTACAGACAGGCAC
AGCAGACAGGTGTTGATGCAAAGGCCGCTGATACTGAGAATAAGGCTTATCCTGTATAG

Upstream 100 bases:

>100_bases
GCTATGGGACCTAACGTAGCCGGTGTTATTGGAACTGCCGTAGCGGCCGGATGCTTCATGGCTATATTCGGTATTTAGAT
TTAATTAAGGAGGATTAATC

Downstream 100 bases:

>100_bases
TATTTAAAAATATAATTCATGCTTTTGGATTTCAAGCAGGTCCGGCTTTGCCGGGCCTGTTTTTTATATTATGAGGATAG
GGTGACTCGTAAGTCCAGGG

Product: oxaloacetate decarboxylase

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSEVVKKPLKITETVLRDAHQSLIATRMTTEQMLPIVDKMDKVGYNAVECWGGATFDACLRFLKEDPWDRLRKLRDGFKN
TKLQMLFRGQNILGYRPYADDVVEYFVQKSIANGIDIIRIFDCLNDIRNLQTAVTACNKEKGHAQVALSYTLGDAYTLDY
WMDMAKRVEDMGADSLCIKDMAGLLVPAKATELVQALKDSTSLPVELHTHYTSGVASMTYMKAVEAGVDIIDTAMSPFAL
GTSQPATEVMVEAFKGTEFDTGLDLNLLSEIADYFRPMREEALASGLMNPKVLGVNINTLRYQVPGGMLSNLISQLKEQG
KEDKYEEVLAEVPRVRKDLGEPPLVTPSSQIVGTQAVFNVLMGERYKVATKETKDILLGKYGQTVKPFNPEVVDKVLGDD
KKNAITCRPADLLEPELDKIEAEMKQWKQQDEDVLSYALFPQVATDFFKYRQAQQTGVDAKAADTENKAYPV

Sequences:

>Translated_472_residues
MSEVVKKPLKITETVLRDAHQSLIATRMTTEQMLPIVDKMDKVGYNAVECWGGATFDACLRFLKEDPWDRLRKLRDGFKN
TKLQMLFRGQNILGYRPYADDVVEYFVQKSIANGIDIIRIFDCLNDIRNLQTAVTACNKEKGHAQVALSYTLGDAYTLDY
WMDMAKRVEDMGADSLCIKDMAGLLVPAKATELVQALKDSTSLPVELHTHYTSGVASMTYMKAVEAGVDIIDTAMSPFAL
GTSQPATEVMVEAFKGTEFDTGLDLNLLSEIADYFRPMREEALASGLMNPKVLGVNINTLRYQVPGGMLSNLISQLKEQG
KEDKYEEVLAEVPRVRKDLGEPPLVTPSSQIVGTQAVFNVLMGERYKVATKETKDILLGKYGQTVKPFNPEVVDKVLGDD
KKNAITCRPADLLEPELDKIEAEMKQWKQQDEDVLSYALFPQVATDFFKYRQAQQTGVDAKAADTENKAYPV
>Mature_471_residues
SEVVKKPLKITETVLRDAHQSLIATRMTTEQMLPIVDKMDKVGYNAVECWGGATFDACLRFLKEDPWDRLRKLRDGFKNT
KLQMLFRGQNILGYRPYADDVVEYFVQKSIANGIDIIRIFDCLNDIRNLQTAVTACNKEKGHAQVALSYTLGDAYTLDYW
MDMAKRVEDMGADSLCIKDMAGLLVPAKATELVQALKDSTSLPVELHTHYTSGVASMTYMKAVEAGVDIIDTAMSPFALG
TSQPATEVMVEAFKGTEFDTGLDLNLLSEIADYFRPMREEALASGLMNPKVLGVNINTLRYQVPGGMLSNLISQLKEQGK
EDKYEEVLAEVPRVRKDLGEPPLVTPSSQIVGTQAVFNVLMGERYKVATKETKDILLGKYGQTVKPFNPEVVDKVLGDDK
KNAITCRPADLLEPELDKIEAEMKQWKQQDEDVLSYALFPQVATDFFKYRQAQQTGVDAKAADTENKAYPV

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=465, Percent_Identity=35.0537634408602, Blast_Score=237, Evalue=2e-62,
Organism=Homo sapiens, GI106049295, Length=465, Percent_Identity=35.0537634408602, Blast_Score=237, Evalue=2e-62,
Organism=Homo sapiens, GI106049292, Length=465, Percent_Identity=35.0537634408602, Blast_Score=237, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI17562816, Length=481, Percent_Identity=34.3035343035343, Blast_Score=253, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6321376, Length=470, Percent_Identity=34.0425531914894, Blast_Score=265, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6319695, Length=462, Percent_Identity=34.4155844155844, Blast_Score=263, Evalue=3e-71,
Organism=Drosophila melanogaster, GI24652212, Length=469, Percent_Identity=35.6076759061834, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24652210, Length=469, Percent_Identity=35.6076759061834, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24652214, Length=469, Percent_Identity=35.6076759061834, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI19921944, Length=469, Percent_Identity=35.6076759061834, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24652216, Length=469, Percent_Identity=35.6076759061834, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI281363050, Length=469, Percent_Identity=35.6076759061834, Blast_Score=256, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24652224, Length=469, Percent_Identity=35.6076759061834, Blast_Score=256, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24652222, Length=469, Percent_Identity=35.6076759061834, Blast_Score=256, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24652220, Length=469, Percent_Identity=35.6076759061834, Blast_Score=256, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24652218, Length=469, Percent_Identity=35.6076759061834, Blast_Score=256, Evalue=3e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 52719; Mature: 52588

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVVKKPLKITETVLRDAHQSLIATRMTTEQMLPIVDKMDKVGYNAVECWGGATFDACL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHH
RFLKEDPWDRLRKLRDGFKNTKLQMLFRGQNILGYRPYADDVVEYFVQKSIANGIDIIRI
HHHHCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHH
FDCLNDIRNLQTAVTACNKEKGHAQVALSYTLGDAYTLDYWMDMAKRVEDMGADSLCIKD
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHH
MAGLLVPAKATELVQALKDSTSLPVELHTHYTSGVASMTYMKAVEAGVDIIDTAMSPFAL
HHHHCCCHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHCHHHHHHCCCCCCC
GTSQPATEVMVEAFKGTEFDTGLDLNLLSEIADYFRPMREEALASGLMNPKVLGVNINTL
CCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEE
RYQVPGGMLSNLISQLKEQGKEDKYEEVLAEVPRVRKDLGEPPLVTPSSQIVGTQAVFNV
EEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
LMGERYKVATKETKDILLGKYGQTVKPFNPEVVDKVLGDDKKNAITCRPADLLEPELDKI
HHCCCEEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCEEEECCHHHCCCHHHHH
EAEMKQWKQQDEDVLSYALFPQVATDFFKYRQAQQTGVDAKAADTENKAYPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SEVVKKPLKITETVLRDAHQSLIATRMTTEQMLPIVDKMDKVGYNAVECWGGATFDACL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHH
RFLKEDPWDRLRKLRDGFKNTKLQMLFRGQNILGYRPYADDVVEYFVQKSIANGIDIIRI
HHHHCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHH
FDCLNDIRNLQTAVTACNKEKGHAQVALSYTLGDAYTLDYWMDMAKRVEDMGADSLCIKD
HHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHH
MAGLLVPAKATELVQALKDSTSLPVELHTHYTSGVASMTYMKAVEAGVDIIDTAMSPFAL
HHHHCCCHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHCHHHHHHCCCCCCC
GTSQPATEVMVEAFKGTEFDTGLDLNLLSEIADYFRPMREEALASGLMNPKVLGVNINTL
CCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEE
RYQVPGGMLSNLISQLKEQGKEDKYEEVLAEVPRVRKDLGEPPLVTPSSQIVGTQAVFNV
EEECCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
LMGERYKVATKETKDILLGKYGQTVKPFNPEVVDKVLGDDKKNAITCRPADLLEPELDKI
HHCCCEEEHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCEEEECCHHHCCCHHHHH
EAEMKQWKQQDEDVLSYALFPQVATDFFKYRQAQQTGVDAKAADTENKAYPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]