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The map label for this gene is cysB [H]

Identifier: 238923886

GI number: 238923886

Start: 1393674

End: 1394579

Strand: Direct

Name: cysB [H]

Synonym: EUBREC_1514

Alternate gene names: 238923886

Gene position: 1393674-1394579 (Clockwise)

Preceding gene: 238923885

Following gene: 238923887

Centisome position: 40.4

GC content: 41.17

Gene sequence:

>906_bases
ATGACACTTCAACAACTCAGACAGGTACTTACAATTGCAGAATCCAATTCCATGAATGAGGCGGCAAAGAAGCTTTTTGT
TTCACAGCCAAATCTGTCCGCCACAGTACGAGAGGTGGAGGAGGAGCTTGGAATCACGGTATTCATGAGAAATAACCGTG
GAATTACAGTCACAGCGGAGGGCGAGGAGTTTTTAGGATACGCCAAACAGGTTGTGGAGCAGTATCATCTGCTTGAGAAC
CGGTATCTGAATGTGGAGTCCAAAAAGAAATTCTCGGTTTCCATGCAGCACTATTCATTTGCAGTAAAGGCCTTTGTACA
GCTTGCTAAAGAGGTGGGCATGGATGAGTACGAGTTTGCTGTCCACGAGACAAAGACGAAAGAGGTAATAGACAACGTAA
AGAACTTAAAGAGTGAGATTGGAGTTCTTTATTTAAGTGATTTCAATGAAAAGGTTTTAAGAAAGCTTTTTAAGGAATGT
GACATCGAGTTTAAAGAGCTCTTTACATGCAATACATATGTGTATCTCTGGAAAAAACATCCGCTTGCCGGCCAAAAAGA
GATTACACTTGATGATTTGCAGGAGTACCCATGTCTGACCTTTGAACAGGGTGTGAACAATTCCTTTTATTATGCTGAGG
AGATGATGAGCACATATGAGTACAAGCGTGTCATAAAGGCTGATGACAGAGCCACAATGCTCAATCTCATGGTGGGGCTC
AACGGATTTACACTGTGCTCAGGTATTATTTCTGAGGATTTAAACGGTGATGACTATGCGGCAATTCCACTTAAGGAAAG
TGAAAAAATGCATATAGGCTATATCAAGCACAAGGGCACGAAGCTTAGCAAGCTCGGTGAAATATATATAGATGCGTTAA
AGAATTACGAGAATAAAGTTTTATAA

Upstream 100 bases:

>100_bases
TTATCTAATATAATACAATTGTGCGGTTTTTACGAAGGTGCCTGGGCGCTGAATAGTTACACATTCTGCTAAAAACCGTC
AGTTCACAATAGGAGAGAAA

Downstream 100 bases:

>100_bases
ATGAAAAATTGTAAAAAATGAATTTAAATTAATTAAATATCGTACCATTTTTGTAAGAATTTCACAGTTATATTGTTTTT
TTGAAAAAACATGCATGAAA

Product: transcriptional regulator, LysR family

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MTLQQLRQVLTIAESNSMNEAAKKLFVSQPNLSATVREVEEELGITVFMRNNRGITVTAEGEEFLGYAKQVVEQYHLLEN
RYLNVESKKKFSVSMQHYSFAVKAFVQLAKEVGMDEYEFAVHETKTKEVIDNVKNLKSEIGVLYLSDFNEKVLRKLFKEC
DIEFKELFTCNTYVYLWKKHPLAGQKEITLDDLQEYPCLTFEQGVNNSFYYAEEMMSTYEYKRVIKADDRATMLNLMVGL
NGFTLCSGIISEDLNGDDYAAIPLKESEKMHIGYIKHKGTKLSKLGEIYIDALKNYENKVL

Sequences:

>Translated_301_residues
MTLQQLRQVLTIAESNSMNEAAKKLFVSQPNLSATVREVEEELGITVFMRNNRGITVTAEGEEFLGYAKQVVEQYHLLEN
RYLNVESKKKFSVSMQHYSFAVKAFVQLAKEVGMDEYEFAVHETKTKEVIDNVKNLKSEIGVLYLSDFNEKVLRKLFKEC
DIEFKELFTCNTYVYLWKKHPLAGQKEITLDDLQEYPCLTFEQGVNNSFYYAEEMMSTYEYKRVIKADDRATMLNLMVGL
NGFTLCSGIISEDLNGDDYAAIPLKESEKMHIGYIKHKGTKLSKLGEIYIDALKNYENKVL
>Mature_300_residues
TLQQLRQVLTIAESNSMNEAAKKLFVSQPNLSATVREVEEELGITVFMRNNRGITVTAEGEEFLGYAKQVVEQYHLLENR
YLNVESKKKFSVSMQHYSFAVKAFVQLAKEVGMDEYEFAVHETKTKEVIDNVKNLKSEIGVLYLSDFNEKVLRKLFKECD
IEFKELFTCNTYVYLWKKHPLAGQKEITLDDLQEYPCLTFEQGVNNSFYYAEEMMSTYEYKRVIKADDRATMLNLMVGLN
GFTLCSGIISEDLNGDDYAAIPLKESEKMHIGYIKHKGTKLSKLGEIYIDALKNYENKVL

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=214, Percent_Identity=25.2336448598131, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787879, Length=210, Percent_Identity=22.3809523809524, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI145693105, Length=302, Percent_Identity=22.1854304635762, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34720; Mature: 34589

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLQQLRQVLTIAESNSMNEAAKKLFVSQPNLSATVREVEEELGITVFMRNNRGITVTAE
CCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEEECCCCEEEEEC
GEEFLGYAKQVVEQYHLLENRYLNVESKKKFSVSMQHYSFAVKAFVQLAKEVGMDEYEFA
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHCCCCHHHHH
VHETKTKEVIDNVKNLKSEIGVLYLSDFNEKVLRKLFKECDIEFKELFTCNTYVYLWKKH
HHHHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHCCCCHHHHEEECEEEEEEECC
PLAGQKEITLDDLQEYPCLTFEQGVNNSFYYAEEMMSTYEYKRVIKADDRATMLNLMVGL
CCCCCCCCCHHHHHHCCCEEHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
NGFTLCSGIISEDLNGDDYAAIPLKESEKMHIGYIKHKGTKLSKLGEIYIDALKNYENKV
CHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCC
L
C
>Mature Secondary Structure 
TLQQLRQVLTIAESNSMNEAAKKLFVSQPNLSATVREVEEELGITVFMRNNRGITVTAE
CHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEEECCCCEEEEEC
GEEFLGYAKQVVEQYHLLENRYLNVESKKKFSVSMQHYSFAVKAFVQLAKEVGMDEYEFA
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHHCCCCHHHHH
VHETKTKEVIDNVKNLKSEIGVLYLSDFNEKVLRKLFKECDIEFKELFTCNTYVYLWKKH
HHHHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHCCCCHHHHEEECEEEEEEECC
PLAGQKEITLDDLQEYPCLTFEQGVNNSFYYAEEMMSTYEYKRVIKADDRATMLNLMVGL
CCCCCCCCCHHHHHHCCCEEHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
NGFTLCSGIISEDLNGDDYAAIPLKESEKMHIGYIKHKGTKLSKLGEIYIDALKNYENKV
CHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]