Click here to switch to the map view.

The map label for this gene is pfkA [H]

Identifier: 238923438

GI number: 238923438

Start: 966052

End: 967038

Strand: Direct

Name: pfkA [H]

Synonym: EUBREC_1058

Alternate gene names: 238923438

Gene position: 966052-967038 (Clockwise)

Preceding gene: 238923437

Following gene: 238923439

Centisome position: 28.0

GC content: 46.71

Gene sequence:

>987_bases
ATGGAGGTTATACCAATGGCAAAAGAGATTAACACAATAGGTGTACTTACAAGTGGTGGTGACGCACCGGGAATGAACGC
AGCGATTCGTGCCGTTGTTCGTCAGGCTCTCTCAAAGGGCAAGAAGGTTAAAGGAATCAAGAGAGGATACGCAGGTCTGT
TAAATGAAGAAATCGTGGATATGGATTCAAAGAGCGTTTCAGACACAATCTCACGCGGTGGTACAATCTTACAGACAGCC
AGATGTAAGGAGTTCGTTACAGCAGAGGGACAGCAGAAGGGTGCTGAAATCTGTAAGAAGCATGGTATCGATGCTATCGT
AGTTATCGGTGGTGACGGATCTTTCCAGGGTGCGCAGAAGCTTGCTGCACTTGGCATCAATACAATCGGTCTTCCGGGCA
CAATCGACCTTGATATCGCATGTACAGATTACACAATCGGTTTTGATACAGCCTGCAACACAGCTATGGAGGCTATTGAC
AAGGTACGTGATACATCTACATCACACGAGAGATGCTCAATCATTGAGGTTATGGGACGTGGAGCAGGATACATTGCATT
ATGGTGTGGTCTTGCAAACGGCGCTGAGGAAGTATTACTTCCTGAGAAGTATGACAACAACCTTGAGGCACTCATCGAGA
GAGTAGCTCATGCAAGGGAGCTCGGCAAGCAGCATTACATCATCATCAATGCTGAGGGCGTAGGACATTCACAGGAGATG
GCTAAGGAAATCGAGGCTGCTACAGGCATCGAGACTCGAGCAACTATCCTTGGACATATGCAAAGAGGTGGAAGCCCTTC
ATGTAAGGACCGTGTATACGCTACAATCATGGGCGCTATGGCAGTTGATTTACTCTGCGAGGGCAAGTCAAACAGAGTTA
TTGCCCACAAGGATGGCAATTTTGTAGATTTCGATATCGACGAGGCACTTGCTATGAAGAAGAGTGTAGACGAGTACATG
TACGATATCTCTAAATCTCTGGTTTAG

Upstream 100 bases:

>100_bases
AGACAAAGAAAATGCGAAAATTGTTGAAAAGAATATTGAAAAAATGTGCTAAAACAAGTACAATAGATTGGGATTAGAAT
AAATGATAATTTTTTAACAT

Downstream 100 bases:

>100_bases
TATCTAATATATTTACGATAAATATGAATGCCCTTGTATCCGGCACATCAAATGCAGGTAATTGCGTGCATGTGCCGGTG
CAGGGGCATTTATGCATTCG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MEVIPMAKEINTIGVLTSGGDAPGMNAAIRAVVRQALSKGKKVKGIKRGYAGLLNEEIVDMDSKSVSDTISRGGTILQTA
RCKEFVTAEGQQKGAEICKKHGIDAIVVIGGDGSFQGAQKLAALGINTIGLPGTIDLDIACTDYTIGFDTACNTAMEAID
KVRDTSTSHERCSIIEVMGRGAGYIALWCGLANGAEEVLLPEKYDNNLEALIERVAHARELGKQHYIIINAEGVGHSQEM
AKEIEAATGIETRATILGHMQRGGSPSCKDRVYATIMGAMAVDLLCEGKSNRVIAHKDGNFVDFDIDEALAMKKSVDEYM
YDISKSLV

Sequences:

>Translated_328_residues
MEVIPMAKEINTIGVLTSGGDAPGMNAAIRAVVRQALSKGKKVKGIKRGYAGLLNEEIVDMDSKSVSDTISRGGTILQTA
RCKEFVTAEGQQKGAEICKKHGIDAIVVIGGDGSFQGAQKLAALGINTIGLPGTIDLDIACTDYTIGFDTACNTAMEAID
KVRDTSTSHERCSIIEVMGRGAGYIALWCGLANGAEEVLLPEKYDNNLEALIERVAHARELGKQHYIIINAEGVGHSQEM
AKEIEAATGIETRATILGHMQRGGSPSCKDRVYATIMGAMAVDLLCEGKSNRVIAHKDGNFVDFDIDEALAMKKSVDEYM
YDISKSLV
>Mature_328_residues
MEVIPMAKEINTIGVLTSGGDAPGMNAAIRAVVRQALSKGKKVKGIKRGYAGLLNEEIVDMDSKSVSDTISRGGTILQTA
RCKEFVTAEGQQKGAEICKKHGIDAIVVIGGDGSFQGAQKLAALGINTIGLPGTIDLDIACTDYTIGFDTACNTAMEAID
KVRDTSTSHERCSIIEVMGRGAGYIALWCGLANGAEEVLLPEKYDNNLEALIERVAHARELGKQHYIIINAEGVGHSQEM
AKEIEAATGIETRATILGHMQRGGSPSCKDRVYATIMGAMAVDLLCEGKSNRVIAHKDGNFVDFDIDEALAMKKSVDEYM
YDISKSLV

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI48762920, Length=347, Percent_Identity=40.3458213256484, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI11321601, Length=345, Percent_Identity=39.7101449275362, Blast_Score=198, Evalue=4e-51,
Organism=Homo sapiens, GI266453619, Length=345, Percent_Identity=37.9710144927536, Blast_Score=194, Evalue=8e-50,
Organism=Homo sapiens, GI266453768, Length=345, Percent_Identity=37.9710144927536, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI266453748, Length=345, Percent_Identity=37.9710144927536, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI4505749, Length=345, Percent_Identity=37.9710144927536, Blast_Score=194, Evalue=1e-49,
Organism=Escherichia coli, GI1790350, Length=319, Percent_Identity=50.1567398119122, Blast_Score=282, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI17558788, Length=312, Percent_Identity=37.8205128205128, Blast_Score=186, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI25147584, Length=325, Percent_Identity=37.8461538461538, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI72003601, Length=292, Percent_Identity=28.4246575342466, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6323861, Length=353, Percent_Identity=34.5609065155807, Blast_Score=180, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6321679, Length=312, Percent_Identity=38.1410256410256, Blast_Score=177, Evalue=3e-45,
Organism=Drosophila melanogaster, GI17647809, Length=325, Percent_Identity=40.3076923076923, Blast_Score=190, Evalue=9e-49,
Organism=Drosophila melanogaster, GI24652337, Length=325, Percent_Identity=40.3076923076923, Blast_Score=190, Evalue=9e-49,
Organism=Drosophila melanogaster, GI28573326, Length=324, Percent_Identity=38.5802469135802, Blast_Score=185, Evalue=3e-47,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 35014; Mature: 35014

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVIPMAKEINTIGVLTSGGDAPGMNAAIRAVVRQALSKGKKVKGIKRGYAGLLNEEIVD
CCCCCCHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHC
MDSKSVSDTISRGGTILQTARCKEFVTAEGQQKGAEICKKHGIDAIVVIGGDGSFQGAQK
CCCHHHHHHHHCCCCEEEHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
LAALGINTIGLPGTIDLDIACTDYTIGFDTACNTAMEAIDKVRDTSTSHERCSIIEVMGR
HHHHCCCCCCCCCEEEEEEEEEEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
GAGYIALWCGLANGAEEVLLPEKYDNNLEALIERVAHARELGKQHYIIINAEGVGHSQEM
CCCEEEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHH
AKEIEAATGIETRATILGHMQRGGSPSCKDRVYATIMGAMAVDLLCEGKSNRVIAHKDGN
HHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCC
FVDFDIDEALAMKKSVDEYMYDISKSLV
EEEECHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEVIPMAKEINTIGVLTSGGDAPGMNAAIRAVVRQALSKGKKVKGIKRGYAGLLNEEIVD
CCCCCCHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHC
MDSKSVSDTISRGGTILQTARCKEFVTAEGQQKGAEICKKHGIDAIVVIGGDGSFQGAQK
CCCHHHHHHHHCCCCEEEHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
LAALGINTIGLPGTIDLDIACTDYTIGFDTACNTAMEAIDKVRDTSTSHERCSIIEVMGR
HHHHCCCCCCCCCEEEEEEEEEEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
GAGYIALWCGLANGAEEVLLPEKYDNNLEALIERVAHARELGKQHYIIINAEGVGHSQEM
CCCEEEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHH
AKEIEAATGIETRATILGHMQRGGSPSCKDRVYATIMGAMAVDLLCEGKSNRVIAHKDGN
HHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCC
FVDFDIDEALAMKKSVDEYMYDISKSLV
EEEECHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA