Click here to switch to the map view.

The map label for this gene is nadB [H]

Identifier: 238923414

GI number: 238923414

Start: 928884

End: 930221

Strand: Direct

Name: nadB [H]

Synonym: EUBREC_1034

Alternate gene names: 238923414

Gene position: 928884-930221 (Clockwise)

Preceding gene: 238923412

Following gene: 238923415

Centisome position: 26.93

GC content: 46.64

Gene sequence:

>1338_bases
ATGAATACAGATGTACTTATTGTGGGAAGCGGCTGCTCAGGTCTTTATTGTGCGCTTAAGCTTCCAAGAGACATAAGAAT
TACACTGATAACAAAGTCAGATCTGGAGAGCAATGATTCATTTCTGGCGCAGGGCGGCATGTGCATGCTTAAAGAGCAGT
CTGATTTCGACAGCTTTTTTGAGGATACCTTAAAGGCCGGCCACTACGAGAATGACAAGGAGTCGGTTGGGATAATGATA
AACTCATCCCCTGAGGTGGTTAAGGATCTTGTTTCATACGGAGCAGATTTTGCGAGAAATGATGACGGCTCACTTGCCTA
TACGAGAGAGGGAGCTCATTCACACAACAGAATTATTTTCCATGAAGACGTGACCGGAAAAGAGATAACAAGCACATTGC
TTGCACAGGTTAAGAAGCTTTCAAATGTGACACTCATGGAGTATACAACGATGGTTGACATCATCGAAAGGGATAACAAA
TGCTACGGCGCCATCATCAGAAAACAGGATGGCACGCTGGAAAAGGTGACAGCGGCATACACTGTCATGGCCTGTGGCGG
AGTTGGAGGACTGTACAGATTTTCAACAAATTTCAGACATCTGACAGGTGACTCACTTGCCATTGCAAAAAAGCATGACA
TAGAGCTTAAAAACCCAGACTATGTGCAGATTCACCCAACGACCTTTTACACGAAAAAGCATGAGGACAGAAGCTTTCTC
ATTTCAGAGTCCGTAAGAGGTGAGGGTGCTAAGCTTTTAGACAAGAATATGAACAGGTTTGTCGATGAGCTCTTACCAAG
AGACGTGCTGACCGGCAAAATCCGTGAGCAGATGAAAAAGGACGGCACAGACTTTGTCTGGGAGGATTTGCGCACCATTC
CAAGGGATGAGCTTGTATCACATTTCCCAAATATCATTGAACATTGCAAGGAGATGGGATATGATGTCTTCAAGGAGCCT
ATTCCTGTTGTTCCGGCACAGCACTATTTCATGGGCGGTGTCAAGGTGAATCACCACAGCCGCACATCCATGGAAAGTCT
CTATGCAGTTGGAGAGACCGCTTGTAACGGAGTGCACGGAAGAAACCGTCTTGCAAGCAATTCACTGCTTGAGAGCCTGG
TATTCGCAAAGCGTGCAGCAGGACAGATGGCGGAGCTTGGCTTTGTAAATGATGAGATAGCAGCCAAAAAGGCCGCAGAT
AGCATCGACCTCGCAGACTACAGCGATGAAAAAGCTGTAGAGAGAGAATACAGGAAGCTCGCCATGGAGGCAATAGAGGG
CCGCACCGGCGCACAAGACATGAATGCCGAATCAGGTGTCACCTACATTCAGGCATAA

Upstream 100 bases:

>100_bases
GTGTTATAATACGCCCATTACAGTAAATCGCATCCAGGGGGATGTTGCTAAAGAAGCGGCTACATCGGTGGATGCAGGTA
AATAAAAGAGGTAACATACT

Downstream 100 bases:

>100_bases
AAGCAAGTATCACCCCGGCACCACACAGCATAAAAAGTGCGAAACCGTAATAAATAATAATTTGAACGAAAGGAATTTAA
ATATATGTACGATCAGGTAA

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MNTDVLIVGSGCSGLYCALKLPRDIRITLITKSDLESNDSFLAQGGMCMLKEQSDFDSFFEDTLKAGHYENDKESVGIMI
NSSPEVVKDLVSYGADFARNDDGSLAYTREGAHSHNRIIFHEDVTGKEITSTLLAQVKKLSNVTLMEYTTMVDIIERDNK
CYGAIIRKQDGTLEKVTAAYTVMACGGVGGLYRFSTNFRHLTGDSLAIAKKHDIELKNPDYVQIHPTTFYTKKHEDRSFL
ISESVRGEGAKLLDKNMNRFVDELLPRDVLTGKIREQMKKDGTDFVWEDLRTIPRDELVSHFPNIIEHCKEMGYDVFKEP
IPVVPAQHYFMGGVKVNHHSRTSMESLYAVGETACNGVHGRNRLASNSLLESLVFAKRAAGQMAELGFVNDEIAAKKAAD
SIDLADYSDEKAVEREYRKLAMEAIEGRTGAQDMNAESGVTYIQA

Sequences:

>Translated_445_residues
MNTDVLIVGSGCSGLYCALKLPRDIRITLITKSDLESNDSFLAQGGMCMLKEQSDFDSFFEDTLKAGHYENDKESVGIMI
NSSPEVVKDLVSYGADFARNDDGSLAYTREGAHSHNRIIFHEDVTGKEITSTLLAQVKKLSNVTLMEYTTMVDIIERDNK
CYGAIIRKQDGTLEKVTAAYTVMACGGVGGLYRFSTNFRHLTGDSLAIAKKHDIELKNPDYVQIHPTTFYTKKHEDRSFL
ISESVRGEGAKLLDKNMNRFVDELLPRDVLTGKIREQMKKDGTDFVWEDLRTIPRDELVSHFPNIIEHCKEMGYDVFKEP
IPVVPAQHYFMGGVKVNHHSRTSMESLYAVGETACNGVHGRNRLASNSLLESLVFAKRAAGQMAELGFVNDEIAAKKAAD
SIDLADYSDEKAVEREYRKLAMEAIEGRTGAQDMNAESGVTYIQA
>Mature_445_residues
MNTDVLIVGSGCSGLYCALKLPRDIRITLITKSDLESNDSFLAQGGMCMLKEQSDFDSFFEDTLKAGHYENDKESVGIMI
NSSPEVVKDLVSYGADFARNDDGSLAYTREGAHSHNRIIFHEDVTGKEITSTLLAQVKKLSNVTLMEYTTMVDIIERDNK
CYGAIIRKQDGTLEKVTAAYTVMACGGVGGLYRFSTNFRHLTGDSLAIAKKHDIELKNPDYVQIHPTTFYTKKHEDRSFL
ISESVRGEGAKLLDKNMNRFVDELLPRDVLTGKIREQMKKDGTDFVWEDLRTIPRDELVSHFPNIIEHCKEMGYDVFKEP
IPVVPAQHYFMGGVKVNHHSRTSMESLYAVGETACNGVHGRNRLASNSLLESLVFAKRAAGQMAELGFVNDEIAAKKAAD
SIDLADYSDEKAVEREYRKLAMEAIEGRTGAQDMNAESGVTYIQA

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=404, Percent_Identity=30.6930693069307, Blast_Score=170, Evalue=2e-42,
Organism=Escherichia coli, GI1788928, Length=398, Percent_Identity=36.9346733668342, Blast_Score=255, Evalue=3e-69,
Organism=Escherichia coli, GI1790597, Length=387, Percent_Identity=31.5245478036176, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1786942, Length=403, Percent_Identity=30.5210918114144, Blast_Score=147, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI17550100, Length=391, Percent_Identity=29.6675191815857, Blast_Score=155, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17505833, Length=391, Percent_Identity=28.9002557544757, Blast_Score=154, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI71986328, Length=245, Percent_Identity=30.2040816326531, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6322416, Length=399, Percent_Identity=30.0751879699248, Blast_Score=155, Evalue=8e-39,
Organism=Saccharomyces cerevisiae, GI6322701, Length=381, Percent_Identity=29.9212598425197, Blast_Score=150, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6320788, Length=472, Percent_Identity=27.3305084745763, Blast_Score=106, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6322511, Length=478, Percent_Identity=26.3598326359833, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI17137288, Length=399, Percent_Identity=32.0802005012531, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24655642, Length=399, Percent_Identity=32.0802005012531, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24655647, Length=399, Percent_Identity=32.0802005012531, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24663005, Length=400, Percent_Identity=30, Blast_Score=149, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027 [H]

Pfam domain/function: PF00890 FAD_binding_2 [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 49619; Mature: 49619

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTDVLIVGSGCSGLYCALKLPRDIRITLITKSDLESNDSFLAQGGMCMLKEQSDFDSFF
CCCCEEEEECCCCCEEEEEECCCCEEEEEEECCCCCCCCCEECCCCEEEEECCCCHHHHH
EDTLKAGHYENDKESVGIMINSSPEVVKDLVSYGADFARNDDGSLAYTREGAHSHNRIIF
HHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCEEEE
HEDVTGKEITSTLLAQVKKLSNVTLMEYTTMVDIIERDNKCYGAIIRKQDGTLEKVTAAY
ECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHH
TVMACGGVGGLYRFSTNFRHLTGDSLAIAKKHDIELKNPDYVQIHPTTFYTKKHEDRSFL
HHHECCCCCCEEEECCCCCEECCCCEEEEECCCCCCCCCCEEEECCCEEEECCCCCCCEE
ISESVRGEGAKLLDKNMNRFVDELLPRDVLTGKIREQMKKDGTDFVWEDLRTIPRDELVS
EECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHH
HFPNIIEHCKEMGYDVFKEPIPVVPAQHYFMGGVKVNHHSRTSMESLYAVGETACNGVHG
HHHHHHHHHHHCCHHHHHCCCCCCCCCHHEECCEEEECCCHHHHHHHHHHHHHHHCCCCC
RNRLASNSLLESLVFAKRAAGQMAELGFVNDEIAAKKAADSIDLADYSDEKAVEREYRKL
CHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
AMEAIEGRTGAQDMNAESGVTYIQA
HHHHHCCCCCCCCCCCCCCEEEEEC
>Mature Secondary Structure
MNTDVLIVGSGCSGLYCALKLPRDIRITLITKSDLESNDSFLAQGGMCMLKEQSDFDSFF
CCCCEEEEECCCCCEEEEEECCCCEEEEEEECCCCCCCCCEECCCCEEEEECCCCHHHHH
EDTLKAGHYENDKESVGIMINSSPEVVKDLVSYGADFARNDDGSLAYTREGAHSHNRIIF
HHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCEEEE
HEDVTGKEITSTLLAQVKKLSNVTLMEYTTMVDIIERDNKCYGAIIRKQDGTLEKVTAAY
ECCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHH
TVMACGGVGGLYRFSTNFRHLTGDSLAIAKKHDIELKNPDYVQIHPTTFYTKKHEDRSFL
HHHECCCCCCEEEECCCCCEECCCCEEEEECCCCCCCCCCEEEECCCEEEECCCCCCCEE
ISESVRGEGAKLLDKNMNRFVDELLPRDVLTGKIREQMKKDGTDFVWEDLRTIPRDELVS
EECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHH
HFPNIIEHCKEMGYDVFKEPIPVVPAQHYFMGGVKVNHHSRTSMESLYAVGETACNGVHG
HHHHHHHHHHHCCHHHHHCCCCCCCCCHHEECCEEEECCCHHHHHHHHHHHHHHHCCCCC
RNRLASNSLLESLVFAKRAAGQMAELGFVNDEIAAKKAADSIDLADYSDEKAVEREYRKL
CHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
AMEAIEGRTGAQDMNAESGVTYIQA
HHHHHCCCCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11792842 [H]