BacMap

Definition	Eubacterium rectale ATCC 33656, complete genome.
Accession	NC_012781
Length	3,449,685

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The map label for this gene is crt [H]

Identifier: 238923397

GI number: 238923397

Start: 915745

End: 916548

Strand: Direct

Name: crt [H]

Synonym: EUBREC_1017

Alternate gene names: 238923397

Gene position: 915745-916548 (Clockwise)

Preceding gene: 238923396

Following gene: 238923399

Centisome position: 26.55

GC content: 44.15

Gene sequence:

>804_bases
ATGAGCGAGTTCAAAAATTTAACACTTGACGTAGCAGATCAGATTGCAGTACTTGCAATTTCAAGACCGGCTGCTCTTAA
TGCATTAAACAGTGAGACTCTTGATGAGTTAAATGTAGCATTAACAGAGATAGAGGCAAGAGACGATGTTAAGGTGCTTA
TCCTTACAGGTGGTCCTGACAAGAAGGGCAACGAGTTCAAGTCATTCGTAGCAGGAGCAGACATCTCAGAGATGGTTAAT
TTCACAGCACCACAGGCAAGAGCATTCGGAATCAAGGCTTCAGAGCCATTCTTCAAGCTCATGAATATGCGTCAGGTAAC
AATCGCAGCTGTCAATGGATTTGCACTCGGTGGAGGATGCGAGATTTCTATGGCATGTGATATCAGAATCGCTTCTGAGA
ACGCAATTTTCGGACAGCCTGAGACAGGACTTGGAATCATCCCTGGCTTTGGCGGTACACAGAGACTTGCTCGTTTAGTA
GGAATGGGACGTGCTAAGGAAATGATCTTCACATGCGACAATGTAGACGCTGCAGAAGCATACCGCATAGGTCTCGTAAA
TAAAGTAGTAGCAAAAGAGGAGCTTATGCCAACAGCTAAGGCTATGGCTGCAAAGATCATTTCTAAGGGAAGCTACGCAA
TCTCAGTTGCAAAGGCTGCTATCAACAACGGATATGATATGGATATCAAGAACGCTGTTGAGATGGAAGCAAACCTCTTT
GGTGTTGTAAATGATACAAACGACAAGAAAGAGGGAATGGGAGCATTCCTTGAGAAGAGAGCTGCAAACCTCACAGATTT
CTAA

Upstream 100 bases:

>100_bases
ATTCAGACATTGACAAAAATTTGTCAAAGTGTTACTATTTTTTTATAAATTACGACAGAAATGTCAGTTATACATAGGTT
TTTTAAGGAGGATTTATACA

Downstream 100 bases:

>100_bases
ATAATGATTTGAATTATTTATTGTATATGAATTAAAATAGCCGGTGTGTTAAGCACCGGCTATTTTGTATGAATCAGATT
AGATTATTACTCTCACGATA

Product: 3-hydroxybutyryl-coA dehydratase

Products: NA

Alternate protein names: Crotonase [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MSEFKNLTLDVADQIAVLAISRPAALNALNSETLDELNVALTEIEARDDVKVLILTGGPDKKGNEFKSFVAGADISEMVN
FTAPQARAFGIKASEPFFKLMNMRQVTIAAVNGFALGGGCEISMACDIRIASENAIFGQPETGLGIIPGFGGTQRLARLV
GMGRAKEMIFTCDNVDAAEAYRIGLVNKVVAKEELMPTAKAMAAKIISKGSYAISVAKAAINNGYDMDIKNAVEMEANLF
GVVNDTNDKKEGMGAFLEKRAANLTDF

Sequences:

>Translated_267_residues
MSEFKNLTLDVADQIAVLAISRPAALNALNSETLDELNVALTEIEARDDVKVLILTGGPDKKGNEFKSFVAGADISEMVN
FTAPQARAFGIKASEPFFKLMNMRQVTIAAVNGFALGGGCEISMACDIRIASENAIFGQPETGLGIIPGFGGTQRLARLV
GMGRAKEMIFTCDNVDAAEAYRIGLVNKVVAKEELMPTAKAMAAKIISKGSYAISVAKAAINNGYDMDIKNAVEMEANLF
GVVNDTNDKKEGMGAFLEKRAANLTDF
>Mature_266_residues
SEFKNLTLDVADQIAVLAISRPAALNALNSETLDELNVALTEIEARDDVKVLILTGGPDKKGNEFKSFVAGADISEMVNF
TAPQARAFGIKASEPFFKLMNMRQVTIAAVNGFALGGGCEISMACDIRIASENAIFGQPETGLGIIPGFGGTQRLARLVG
MGRAKEMIFTCDNVDAAEAYRIGLVNKVVAKEELMPTAKAMAAKIISKGSYAISVAKAAINNGYDMDIKNAVEMEANLFG
VVNDTNDKKEGMGAFLEKRAANLTDF

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=254, Percent_Identity=41.7322834645669, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI4502327, Length=251, Percent_Identity=35.4581673306773, Blast_Score=117, Evalue=8e-27,
Organism=Homo sapiens, GI150378541, Length=264, Percent_Identity=32.1969696969697, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI20127408, Length=206, Percent_Identity=33.0097087378641, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI31542718, Length=263, Percent_Identity=29.6577946768061, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI68989263, Length=190, Percent_Identity=31.5789473684211, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI157694516, Length=250, Percent_Identity=24, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI213417737, Length=250, Percent_Identity=24, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI62530384, Length=234, Percent_Identity=28.2051282051282, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI157694520, Length=205, Percent_Identity=25.3658536585366, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI70995211, Length=227, Percent_Identity=27.3127753303965, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI295842266, Length=232, Percent_Identity=25.8620689655172, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI116235487, Length=196, Percent_Identity=29.5918367346939, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI37594471, Length=222, Percent_Identity=27.9279279279279, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI261878539, Length=76, Percent_Identity=43.421052631579, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI37594469, Length=222, Percent_Identity=27.9279279279279, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI221136756, Length=252, Percent_Identity=25, Blast_Score=64, Evalue=1e-10,
Organism=Homo sapiens, GI221136753, Length=252, Percent_Identity=25, Blast_Score=64, Evalue=1e-10,
Organism=Escherichia coli, GI1787659, Length=268, Percent_Identity=35.4477611940299, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1790281, Length=235, Percent_Identity=37.4468085106383, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI221142681, Length=264, Percent_Identity=32.5757575757576, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1788597, Length=265, Percent_Identity=31.3207547169811, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1788682, Length=205, Percent_Identity=34.1463414634146, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI87082183, Length=268, Percent_Identity=29.4776119402985, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787660, Length=257, Percent_Identity=29.9610894941634, Blast_Score=97, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI25145438, Length=254, Percent_Identity=40.5511811023622, Blast_Score=178, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17554946, Length=250, Percent_Identity=38.4, Blast_Score=164, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17540714, Length=231, Percent_Identity=37.6623376623377, Blast_Score=150, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17558304, Length=181, Percent_Identity=32.5966850828729, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25144276, Length=182, Percent_Identity=31.8681318681319, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17508953, Length=182, Percent_Identity=31.8681318681319, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17508951, Length=182, Percent_Identity=31.8681318681319, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17534483, Length=249, Percent_Identity=27.3092369477912, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI25144160, Length=204, Percent_Identity=28.4313725490196, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI25144157, Length=204, Percent_Identity=28.4313725490196, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17560910, Length=254, Percent_Identity=27.1653543307087, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17536985, Length=219, Percent_Identity=26.4840182648402, Blast_Score=78, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320241, Length=194, Percent_Identity=29.3814432989691, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI20129971, Length=254, Percent_Identity=39.7637795275591, Blast_Score=181, Evalue=5e-46,
Organism=Drosophila melanogaster, GI24653477, Length=254, Percent_Identity=39.7637795275591, Blast_Score=181, Evalue=5e-46,
Organism=Drosophila melanogaster, GI24653139, Length=257, Percent_Identity=36.9649805447471, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24650670, Length=188, Percent_Identity=31.9148936170213, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24654903, Length=234, Percent_Identity=27.3504273504274, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI19922422, Length=254, Percent_Identity=25.5905511811024, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21357171, Length=204, Percent_Identity=28.921568627451, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24583165, Length=263, Percent_Identity=28.5171102661597, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24583077, Length=190, Percent_Identity=35.2631578947368, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24583079, Length=190, Percent_Identity=35.2631578947368, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI19921000, Length=190, Percent_Identity=35.2631578947368, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI19920382, Length=216, Percent_Identity=26.8518518518519, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI19921018, Length=198, Percent_Identity=30.8080808080808, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI28571729, Length=153, Percent_Identity=29.4117647058824, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI28571730, Length=153, Percent_Identity=29.4117647058824, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.55 [H]

Molecular weight: Translated: 28441; Mature: 28310

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEFKNLTLDVADQIAVLAISRPAALNALNSETLDELNVALTEIEARDDVKVLILTGGPD
CCCCCCCCHHHHCCEEEEEECCCHHHHHCCHHHHHHHHHHHEEEECCCCEEEEEEECCCC
KKGNEFKSFVAGADISEMVNFTAPQARAFGIKASEPFFKLMNMRQVTIAAVNGFALGGGC
CCCCHHHHHHCCCCHHHHHHCCCCCHHEECCCCCCHHHHHHCCCEEEEEEECCEEECCCE
EISMACDIRIASENAIFGQPETGLGIIPGFGGTQRLARLVGMGRAKEMIFTCDNVDAAEA
EEEEEEEEEECCCCCCCCCCCCCCEEECCCCCHHHHHHHHCCCCCCEEEEEECCCCHHHH
YRIGLVNKVVAKEELMPTAKAMAAKIISKGSYAISVAKAAINNGYDMDIKNAVEMEANLF
HHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEHHHHCCCCCCCCCHHHEECCEEE
GVVNDTNDKKEGMGAFLEKRAANLTDF
EEEECCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SEFKNLTLDVADQIAVLAISRPAALNALNSETLDELNVALTEIEARDDVKVLILTGGPD
CCCCCCCHHHHCCEEEEEECCCHHHHHCCHHHHHHHHHHHEEEECCCCEEEEEEECCCC
KKGNEFKSFVAGADISEMVNFTAPQARAFGIKASEPFFKLMNMRQVTIAAVNGFALGGGC
CCCCHHHHHHCCCCHHHHHHCCCCCHHEECCCCCCHHHHHHCCCEEEEEEECCEEECCCE
EISMACDIRIASENAIFGQPETGLGIIPGFGGTQRLARLVGMGRAKEMIFTCDNVDAAEA
EEEEEEEEEECCCCCCCCCCCCCCEEECCCCCHHHHHHHHCCCCCCEEEEEECCCCHHHH
YRIGLVNKVVAKEELMPTAKAMAAKIISKGSYAISVAKAAINNGYDMDIKNAVEMEANLF
HHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEHHHHCCCCCCCCCHHHEECCEEE
GVVNDTNDKKEGMGAFLEKRAANLTDF
EEEECCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8655474; 11466286 [H]