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The map label for this gene is ispD [H]

Identifier: 238923245

GI number: 238923245

Start: 761746

End: 762465

Strand: Direct

Name: ispD [H]

Synonym: EUBREC_0842

Alternate gene names: 238923245

Gene position: 761746-762465 (Clockwise)

Preceding gene: 238923244

Following gene: 238923246

Centisome position: 22.08

GC content: 41.25

Gene sequence:

>720_bases
ATGGATAAGAAGAAATACACAGCAATCATACTGTCAGCCGGTACAGGAAGCCGGATGAAAAGTAATATGCCAAAGCAGTA
TATGGAGCTTTTGGGGCAGCCTGTGATTTATTATAGTATAAAAGCATTTGAGGATAGTCCGGTGGATGAAATAGTTATAG
TCTGCAGTGCGGATTATATTGAGTATTTCAGACATTCGATAATTGAAAAATATGGCTTTAAGAAGGTGAAAGCAATAGTG
CAGGGGGGCAAAGAACGCTACAACTCTGTATATGAGGGGCTTAAAGCAGCAAACGGCACGGACTATGTGCTGATCCATGA
CGGCGCACGTCCTCTGGTGGATAATGAAATAATAGTCCGTTCTATGCATACGGTTGAAAAAGAAAAAGCATGCGTAGCAG
GAATGCCTGTAAAGGACACAATAAAGGTGTCAGATGAATTGGGATATTCAGCAAATACTCCTGACAGGAAAAGCCTGTGG
CAGATTCAGACACCGCAATGCTTTGAATATGATTTGCTGGCTCAGTCCTATGATAAGCTGTTTAGTGACATGTCATGTGG
GAAAAGTGTGCCGGCAATTACGGATGATGCAATGATAGTAGAGTATGGGAGTGACACAAAAGTAAGGCTCATAGAGGGCT
CGTATGAAAATATAAAGGTCACAACTCCTGAAGATATGGGGATTGCAGAACTTTTTTTAAAGAAACGCAGAAAAATGTAA

Upstream 100 bases:

>100_bases
CCGAATTTAAAACTTGGTGAATGATTCTGTAATTGCTTCCGGTTACACCGGTGTGCAAACATAATGTGCAGATTTATCAT
GGATAGGAAAATAAAGGAAT

Downstream 100 bases:

>100_bases
AAAAATTAGAAATTAGTGTTGACATATTGTAACATTGATGATAATATAAATCCTGCGCTGAACAAGTGCAGACAACTAAA
CAATTAAGTATTAATTGTGA

Product: 4-diphosphocytidyl-2-methylerithritol synthase (sugar nucleotide phosphorylase family)

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MDKKKYTAIILSAGTGSRMKSNMPKQYMELLGQPVIYYSIKAFEDSPVDEIVIVCSADYIEYFRHSIIEKYGFKKVKAIV
QGGKERYNSVYEGLKAANGTDYVLIHDGARPLVDNEIIVRSMHTVEKEKACVAGMPVKDTIKVSDELGYSANTPDRKSLW
QIQTPQCFEYDLLAQSYDKLFSDMSCGKSVPAITDDAMIVEYGSDTKVRLIEGSYENIKVTTPEDMGIAELFLKKRRKM

Sequences:

>Translated_239_residues
MDKKKYTAIILSAGTGSRMKSNMPKQYMELLGQPVIYYSIKAFEDSPVDEIVIVCSADYIEYFRHSIIEKYGFKKVKAIV
QGGKERYNSVYEGLKAANGTDYVLIHDGARPLVDNEIIVRSMHTVEKEKACVAGMPVKDTIKVSDELGYSANTPDRKSLW
QIQTPQCFEYDLLAQSYDKLFSDMSCGKSVPAITDDAMIVEYGSDTKVRLIEGSYENIKVTTPEDMGIAELFLKKRRKM
>Mature_239_residues
MDKKKYTAIILSAGTGSRMKSNMPKQYMELLGQPVIYYSIKAFEDSPVDEIVIVCSADYIEYFRHSIIEKYGFKKVKAIV
QGGKERYNSVYEGLKAANGTDYVLIHDGARPLVDNEIIVRSMHTVEKEKACVAGMPVKDTIKVSDELGYSANTPDRKSLW
QIQTPQCFEYDLLAQSYDKLFSDMSCGKSVPAITDDAMIVEYGSDTKVRLIEGSYENIKVTTPEDMGIAELFLKKRRKM

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) [H]

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=241, Percent_Identity=27.3858921161826, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI157671913, Length=135, Percent_Identity=31.1111111111111, Blast_Score=74, Evalue=8e-14,
Organism=Escherichia coli, GI1789104, Length=232, Percent_Identity=34.9137931034483, Blast_Score=125, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228 [H]

Pfam domain/function: PF01128 IspD [H]

EC number: =2.7.7.60 [H]

Molecular weight: Translated: 26993; Mature: 26993

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKKKYTAIILSAGTGSRMKSNMPKQYMELLGQPVIYYSIKAFEDSPVDEIVIVCSADYI
CCCCEEEEEEEECCCCCHHHHCCCHHHHHHHCCCEEEEEEEECCCCCCCCEEEEECHHHH
EYFRHSIIEKYGFKKVKAIVQGGKERYNSVYEGLKAANGTDYVLIHDGARPLVDNEIIVR
HHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHH
SMHTVEKEKACVAGMPVKDTIKVSDELGYSANTPDRKSLWQIQTPQCFEYDLLAQSYDKL
HHHHHHHHHHHHCCCCCCHHEEEHHHCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHH
FSDMSCGKSVPAITDDAMIVEYGSDTKVRLIEGSYENIKVTTPEDMGIAELFLKKRRKM
HHHHHCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCEEEECCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MDKKKYTAIILSAGTGSRMKSNMPKQYMELLGQPVIYYSIKAFEDSPVDEIVIVCSADYI
CCCCEEEEEEEECCCCCHHHHCCCHHHHHHHCCCEEEEEEEECCCCCCCCEEEEECHHHH
EYFRHSIIEKYGFKKVKAIVQGGKERYNSVYEGLKAANGTDYVLIHDGARPLVDNEIIVR
HHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHH
SMHTVEKEKACVAGMPVKDTIKVSDELGYSANTPDRKSLWQIQTPQCFEYDLLAQSYDKL
HHHHHHHHHHHHCCCCCCHHEEEHHHCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHH
FSDMSCGKSVPAITDDAMIVEYGSDTKVRLIEGSYENIKVTTPEDMGIAELFLKKRRKM
HHHHHCCCCCCCCCCCEEEEEECCCCEEEEEECCCCCEEEECCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA