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The map label for this gene is thlA [H]

Identifier: 238923140

GI number: 238923140

Start: 677347

End: 678531

Strand: Direct

Name: thlA [H]

Synonym: EUBREC_0733

Alternate gene names: 238923140

Gene position: 677347-678531 (Clockwise)

Preceding gene: 238923139

Following gene: 238923141

Centisome position: 19.64

GC content: 46.33

Gene sequence:

>1185_bases
ATGGCAAAGAAAGTTGTAATCGCAAGTGCTTGCCGTACAGCAATCGGCAAAATGGGCGGAGCATTAAGCAATACTCCAGC
CGCAGATTTAGGTGCAATCGTAATTAAGGAGGCTTTAAACCGTGCAGGTGTTGCTCCTGAGATGGTTGACGAGGTTAAGA
TGGGTTGTGTTATCCAGGCAGCTCAGGGACAGAACGTAGCTCGTCAGGCTTCTATCAAGGCAGGACTTCCAATCGAGGTT
CCGGCTATCACAATCAATGTCGTTTGTGGATCAGGTCTTAAGTGTGTAAACGACGCTGCTACAATGATCAAGGCCGGTGA
GGCTGATATCGTTGTTGCCGGTGGTATGGAGAACATGTCAATGGCTCCTTACGCTATGACAAAGGCACGTTTCGGATACA
GAATGAACAATGCAACAATCATCGATACAATGGTTAACGATGCTCTTACAGATGCATTCAACCACTATCATATGATGATC
ACAGCTGAGAATGTATGTGATAAATACGGCATCACTCGTGAAGAGCTTGATGAGTTCTCAGCAAACTCACAGCAGAAGTG
TGAGGCAGCTATCGCAGCAGGCAAGTTCGATGATGAGATCGTTCCTGTACCTGTTAAGGTTAAGAAAGAAATCGTTGAGT
TCAAAAAGGACGAGGGACCTCGTGCCGGAACAACAGTAGAGACTCTTTCTAAGCTTCGTTGCTGCTCAGGTAAAGAGGGC
GGACTTGTTACAGCCGGTAACGCATCAGGAATCAATGATGGTGCTGCAGCAGTAGTTGTAATGAGCGAAGAGAAAGCTAA
GGAGCTCGGTGTTAAGCCATTAGCTACATGGGTAGCAGGAGCACTTGGCGGAGTTGAGCCTGAGATCATGGGTGTTGGAC
CGGTAGCTTCTACAAAGAAGGTTCTTGCAAAGACAGGTCTTACAATTGATGATTTCGATCTTATCGAGGCAAACGAGGCA
TTTGCTGCACAGTCAATCGCTGTAGCAAGAGATCTTGGTTTCGATATGAGCAAGGTAAATGTAAACGGTGGTGCTATCGC
ACTCGGACATCCTGTAGGAGCTTCAGGCTGTCGTATCCTTGTTACATTATTACATGAGATGCAGAAGAGAGGATCTAACA
GAGGTCTTGCAACACTTTGTATCGGTGGTGGAATGGGATGTTCTACTATCGTTGAGAAGTACTAA

Upstream 100 bases:

>100_bases
TTAACAAAAGCAATTGATTTTTACATAAATTTGTAGTATAATAACTTTGTTAAAAAATTAACAGCACGGATGTGCAATAC
AATTTAAAGGAGATAATATA

Downstream 100 bases:

>100_bases
TTTTCTTTGCCAATAGATATATGAGGGGATGCAGGTGCATCCCCTTGTAGTGTGAATTAGTTTATTCACATCAGTTTATA
TATATGATTAAGTTATGCTG

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MAKKVVIASACRTAIGKMGGALSNTPAADLGAIVIKEALNRAGVAPEMVDEVKMGCVIQAAQGQNVARQASIKAGLPIEV
PAITINVVCGSGLKCVNDAATMIKAGEADIVVAGGMENMSMAPYAMTKARFGYRMNNATIIDTMVNDALTDAFNHYHMMI
TAENVCDKYGITREELDEFSANSQQKCEAAIAAGKFDDEIVPVPVKVKKEIVEFKKDEGPRAGTTVETLSKLRCCSGKEG
GLVTAGNASGINDGAAAVVVMSEEKAKELGVKPLATWVAGALGGVEPEIMGVGPVASTKKVLAKTGLTIDDFDLIEANEA
FAAQSIAVARDLGFDMSKVNVNGGAIALGHPVGASGCRILVTLLHEMQKRGSNRGLATLCIGGGMGCSTIVEKY

Sequences:

>Translated_394_residues
MAKKVVIASACRTAIGKMGGALSNTPAADLGAIVIKEALNRAGVAPEMVDEVKMGCVIQAAQGQNVARQASIKAGLPIEV
PAITINVVCGSGLKCVNDAATMIKAGEADIVVAGGMENMSMAPYAMTKARFGYRMNNATIIDTMVNDALTDAFNHYHMMI
TAENVCDKYGITREELDEFSANSQQKCEAAIAAGKFDDEIVPVPVKVKKEIVEFKKDEGPRAGTTVETLSKLRCCSGKEG
GLVTAGNASGINDGAAAVVVMSEEKAKELGVKPLATWVAGALGGVEPEIMGVGPVASTKKVLAKTGLTIDDFDLIEANEA
FAAQSIAVARDLGFDMSKVNVNGGAIALGHPVGASGCRILVTLLHEMQKRGSNRGLATLCIGGGMGCSTIVEKY
>Mature_393_residues
AKKVVIASACRTAIGKMGGALSNTPAADLGAIVIKEALNRAGVAPEMVDEVKMGCVIQAAQGQNVARQASIKAGLPIEVP
AITINVVCGSGLKCVNDAATMIKAGEADIVVAGGMENMSMAPYAMTKARFGYRMNNATIIDTMVNDALTDAFNHYHMMIT
AENVCDKYGITREELDEFSANSQQKCEAAIAAGKFDDEIVPVPVKVKKEIVEFKKDEGPRAGTTVETLSKLRCCSGKEGG
LVTAGNASGINDGAAAVVVMSEEKAKELGVKPLATWVAGALGGVEPEIMGVGPVASTKKVLAKTGLTIDDFDLIEANEAF
AAQSIAVARDLGFDMSKVNVNGGAIALGHPVGASGCRILVTLLHEMQKRGSNRGLATLCIGGGMGCSTIVEKY

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=390, Percent_Identity=52.0512820512821, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI167614485, Length=393, Percent_Identity=43.5114503816794, Blast_Score=320, Evalue=1e-87,
Organism=Homo sapiens, GI4557237, Length=391, Percent_Identity=42.9667519181586, Blast_Score=291, Evalue=5e-79,
Organism=Homo sapiens, GI4501853, Length=399, Percent_Identity=38.3458646616541, Blast_Score=236, Evalue=4e-62,
Organism=Homo sapiens, GI4504327, Length=426, Percent_Identity=30.5164319248826, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI194353979, Length=393, Percent_Identity=29.2620865139949, Blast_Score=137, Evalue=2e-32,
Organism=Escherichia coli, GI1788554, Length=392, Percent_Identity=57.1428571428571, Blast_Score=401, Evalue=1e-113,
Organism=Escherichia coli, GI87082165, Length=391, Percent_Identity=52.6854219948849, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI1787663, Length=400, Percent_Identity=45.75, Blast_Score=317, Evalue=9e-88,
Organism=Escherichia coli, GI48994986, Length=398, Percent_Identity=40.4522613065327, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1788683, Length=410, Percent_Identity=32.1951219512195, Blast_Score=204, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI133906874, Length=392, Percent_Identity=45.9183673469388, Blast_Score=327, Evalue=6e-90,
Organism=Caenorhabditis elegans, GI25147385, Length=391, Percent_Identity=38.618925831202, Blast_Score=257, Evalue=8e-69,
Organism=Caenorhabditis elegans, GI17535921, Length=393, Percent_Identity=39.440203562341, Blast_Score=255, Evalue=3e-68,
Organism=Caenorhabditis elegans, GI17535917, Length=393, Percent_Identity=30.5343511450382, Blast_Score=168, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17551802, Length=426, Percent_Identity=30.2816901408451, Blast_Score=168, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6325229, Length=401, Percent_Identity=41.8952618453865, Blast_Score=273, Evalue=3e-74,
Organism=Saccharomyces cerevisiae, GI6322031, Length=402, Percent_Identity=38.0597014925373, Blast_Score=231, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24655093, Length=389, Percent_Identity=53.7275064267352, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI17648125, Length=392, Percent_Identity=46.1734693877551, Blast_Score=317, Evalue=6e-87,
Organism=Drosophila melanogaster, GI24640423, Length=392, Percent_Identity=41.8367346938776, Blast_Score=269, Evalue=2e-72,
Organism=Drosophila melanogaster, GI17137578, Length=430, Percent_Identity=29.3023255813954, Blast_Score=174, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 40841; Mature: 40710

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKVVIASACRTAIGKMGGALSNTPAADLGAIVIKEALNRAGVAPEMVDEVKMGCVIQA
CCCCEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCEEEEE
AQGQNVARQASIKAGLPIEVPAITINVVCGSGLKCVNDAATMIKAGEADIVVAGGMENMS
CCCCCHHHHHHCCCCCCEEECEEEEEEEECCCCHHHHHHHHHHCCCCCCEEEECCCCCCC
MAPYAMTKARFGYRMNNATIIDTMVNDALTDAFNHYHMMITAENVCDKYGITREELDEFS
CCCHHHHHHHCCEEECCCEEEHHHHHHHHHHHHCCEEEEEEHHHHHHHHCCCHHHHHHHC
ANSQQKCEAAIAAGKFDDEIVPVPVKVKKEIVEFKKDEGPRAGTTVETLSKLRCCSGKEG
CCCHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCC
GLVTAGNASGINDGAAAVVVMSEEKAKELGVKPLATWVAGALGGVEPEIMGVGPVASTKK
CEEEECCCCCCCCCCEEEEEEECHHHHHCCCHHHHHHHHHHHCCCCCCEEECCCCHHHHH
VLAKTGLTIDDFDLIEANEAFAAQSIAVARDLGFDMSKVNVNGGAIALGHPVGASGCRIL
HHHHCCCCCCCCCCEECCHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCCHHHHH
VTLLHEMQKRGSNRGLATLCIGGGMGCSTIVEKY
HHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHCC
>Mature Secondary Structure 
AKKVVIASACRTAIGKMGGALSNTPAADLGAIVIKEALNRAGVAPEMVDEVKMGCVIQA
CCCEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCEEEEE
AQGQNVARQASIKAGLPIEVPAITINVVCGSGLKCVNDAATMIKAGEADIVVAGGMENMS
CCCCCHHHHHHCCCCCCEEECEEEEEEEECCCCHHHHHHHHHHCCCCCCEEEECCCCCCC
MAPYAMTKARFGYRMNNATIIDTMVNDALTDAFNHYHMMITAENVCDKYGITREELDEFS
CCCHHHHHHHCCEEECCCEEEHHHHHHHHHHHHCCEEEEEEHHHHHHHHCCCHHHHHHHC
ANSQQKCEAAIAAGKFDDEIVPVPVKVKKEIVEFKKDEGPRAGTTVETLSKLRCCSGKEG
CCCHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCC
GLVTAGNASGINDGAAAVVVMSEEKAKELGVKPLATWVAGALGGVEPEIMGVGPVASTKK
CEEEECCCCCCCCCCEEEEEEECHHHHHCCCHHHHHHHHHHHCCCCCCEEECCCCHHHHH
VLAKTGLTIDDFDLIEANEAFAAQSIAVARDLGFDMSKVNVNGGAIALGHPVGASGCRIL
HHHHCCCCCCCCCCEECCHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCCHHHHH
VTLLHEMQKRGSNRGLATLCIGGGMGCSTIVEKY
HHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]