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The map label for this gene is amtB [C]

Identifier: 238923110

GI number: 238923110

Start: 646708

End: 648465

Strand: Direct

Name: amtB [C]

Synonym: EUBREC_0702

Alternate gene names: 238923110

Gene position: 646708-648465 (Clockwise)

Preceding gene: 238923109

Following gene: 238923112

Centisome position: 18.75

GC content: 43.57

Gene sequence:

>1758_bases
ATGACAGAAGAGATTAGTGGAGTAATTTTCGGAGTCTGGTTCCTTATTGGAGCAGCATTGGTTTTCTGGATGCAGGCAGG
TTTTGCTATGGTAGAAGCAGGTTTTACCAGAGCAAAGAATACCGGTAACATCCTTATGAAAAACCTCATGGATTTCTGTA
TTGGTACAGTTGTATTCATACTGATTGGTTTTTCACTTTTATTAGGTGAAGATGTTCTTGGTTTCATCGGTAAACCGGGA
TTTGACATCTTTACATCTTACAAAGATTTTGACTGGTCAAACTTTGTATTCAACCTGGTTTTCTGTGCGACAACAGCAAC
TATCGTATCAGGAGCTATGGCTGAGAGAACAAAATTTATTTCCTACTGTGTTTATTCAGGAGTTATTTCAGCACTTATTT
ATCCAATCGAGGCTCACTGGATCTGGGGTGGTGGCTGGCTTTCACAGATTGGCTTCCATGATTTCGCAGGTTCATGTGCC
ATCCACATGGTTGGTGGTATCAGTGCTCTTGTAGGAGCTGCAATTCTTGGACCTCGTATCGGAAAGTTTACAAAGGATAA
AAACGGAAAGATTACAAAAGTAAACGCTATTCCGGGACATAACATCACAATCGGTGCACTCGGTGTATTCATTCTCTGGC
TTGGCTGGTATGGATTCAACGGTGCAGCAGCAAAATCAGTAGAGCAGCTTGGTTCTATCTTTGTAACAACAACAATCGCA
CCTGCACTTGCAACAGTAGTATGTATGATTTTCACATGGCTCAAATATGGCAAGCCTGATGTGTCAATGTGTCTGAATGC
TTCACTTGCAGGACTTGTAGCTATCACAGCAGGCTGTGATGTAACAGATGCACTTGGTGCTATCATTATCGGTTCCGTAG
CAGGACTTCTCGTCTGCTTCGGTGTATGGTTCCTTGACAATGTACTTCGTGTTGATGACCCTGTAGGTGCTGTCGCAGTA
CATATGATGAATGGTATCTGGGGAACAATCGCTGTTGGTCTTTTTGCCACAAACAGTGCTCCGGGATATTCAATTGCTGA
TTCAAAGGGTAACGAGCTCGTAGGACTTTTCTACGGCGGTGGATTTAAGCTTTTAGGACTCCAGCTCACAGGATTTGTTT
CTGTTGCAGCATGGACAGTAGTTACTATTACAATCGTATTCCTTGTTATCAAGGCTACACTGGGACTTAGAGTTTCAGAG
GAAGAGGAAATCATCGGACTTGACCCAACAGAGCATGGTCTTCCATCAGCATACGCAGGATTTGCTATCATGGATGTATC
AGGTGATGTTATGGATGTCAATGAAAATACAGACCTTGGTTCTTCTGAGTATGCAACAGCTTCTCAGGCAAAGAAGGATG
CAGCAGTGCCTGTTGTAAATGCAACAACACCGGCTTCAGCAAGCGGTATCCACAAGGTTGTTATTATTTCAAAGCTTACA
AAGTACGACAAGCTTCGTAAGGCTATGAATGACTTAGGTGTAACAGGAATGACAGTGACACAGGTTATGGGCTGTGGTAT
CCAGAAGGGTGCCGGCGAGAAATACCGTGGAGCTGAGCTTGATGCGACATTACTTCCAAAGGTTAAGGTTGAGGTAATCG
TTGGCAAGATTCCGGTAGAAAAGGTTATTGAGACTGCTAAGAAGACTCTCTACACCGGACATATCGGAGATGGTAAGATC
TTCGTTTACGACGTGGCACAGGTTGTCAAGGTTCGTACAGGCGAGGAAGGAATTGAGGCATTACAGGATGTAGAGTAA

Upstream 100 bases:

>100_bases
GAAGCTGTGGAAATTGGTGAAGTGTCTCTATTTTTTTATAAATTTTATTTTCGATAAAATGAAAAAATGAAAGAAGAAAC
AGGAAAGAGAGGACATTACT

Downstream 100 bases:

>100_bases
TGCAAATTATTATCTAATCCCCTTAGCATATATACATCAACAAAAACCCCCGAGGATATGATCCTTGGGGGTTTTGCTTT
AGATAATTAAGTTGTGAATC

Product: adenylate/guanylate cyclase

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 585; Mature: 584

Protein sequence:

>585_residues
MTEEISGVIFGVWFLIGAALVFWMQAGFAMVEAGFTRAKNTGNILMKNLMDFCIGTVVFILIGFSLLLGEDVLGFIGKPG
FDIFTSYKDFDWSNFVFNLVFCATTATIVSGAMAERTKFISYCVYSGVISALIYPIEAHWIWGGGWLSQIGFHDFAGSCA
IHMVGGISALVGAAILGPRIGKFTKDKNGKITKVNAIPGHNITIGALGVFILWLGWYGFNGAAAKSVEQLGSIFVTTTIA
PALATVVCMIFTWLKYGKPDVSMCLNASLAGLVAITAGCDVTDALGAIIIGSVAGLLVCFGVWFLDNVLRVDDPVGAVAV
HMMNGIWGTIAVGLFATNSAPGYSIADSKGNELVGLFYGGGFKLLGLQLTGFVSVAAWTVVTITIVFLVIKATLGLRVSE
EEEIIGLDPTEHGLPSAYAGFAIMDVSGDVMDVNENTDLGSSEYATASQAKKDAAVPVVNATTPASASGIHKVVIISKLT
KYDKLRKAMNDLGVTGMTVTQVMGCGIQKGAGEKYRGAELDATLLPKVKVEVIVGKIPVEKVIETAKKTLYTGHIGDGKI
FVYDVAQVVKVRTGEEGIEALQDVE

Sequences:

>Translated_585_residues
MTEEISGVIFGVWFLIGAALVFWMQAGFAMVEAGFTRAKNTGNILMKNLMDFCIGTVVFILIGFSLLLGEDVLGFIGKPG
FDIFTSYKDFDWSNFVFNLVFCATTATIVSGAMAERTKFISYCVYSGVISALIYPIEAHWIWGGGWLSQIGFHDFAGSCA
IHMVGGISALVGAAILGPRIGKFTKDKNGKITKVNAIPGHNITIGALGVFILWLGWYGFNGAAAKSVEQLGSIFVTTTIA
PALATVVCMIFTWLKYGKPDVSMCLNASLAGLVAITAGCDVTDALGAIIIGSVAGLLVCFGVWFLDNVLRVDDPVGAVAV
HMMNGIWGTIAVGLFATNSAPGYSIADSKGNELVGLFYGGGFKLLGLQLTGFVSVAAWTVVTITIVFLVIKATLGLRVSE
EEEIIGLDPTEHGLPSAYAGFAIMDVSGDVMDVNENTDLGSSEYATASQAKKDAAVPVVNATTPASASGIHKVVIISKLT
KYDKLRKAMNDLGVTGMTVTQVMGCGIQKGAGEKYRGAELDATLLPKVKVEVIVGKIPVEKVIETAKKTLYTGHIGDGKI
FVYDVAQVVKVRTGEEGIEALQDVE
>Mature_584_residues
TEEISGVIFGVWFLIGAALVFWMQAGFAMVEAGFTRAKNTGNILMKNLMDFCIGTVVFILIGFSLLLGEDVLGFIGKPGF
DIFTSYKDFDWSNFVFNLVFCATTATIVSGAMAERTKFISYCVYSGVISALIYPIEAHWIWGGGWLSQIGFHDFAGSCAI
HMVGGISALVGAAILGPRIGKFTKDKNGKITKVNAIPGHNITIGALGVFILWLGWYGFNGAAAKSVEQLGSIFVTTTIAP
ALATVVCMIFTWLKYGKPDVSMCLNASLAGLVAITAGCDVTDALGAIIIGSVAGLLVCFGVWFLDNVLRVDDPVGAVAVH
MMNGIWGTIAVGLFATNSAPGYSIADSKGNELVGLFYGGGFKLLGLQLTGFVSVAAWTVVTITIVFLVIKATLGLRVSEE
EEIIGLDPTEHGLPSAYAGFAIMDVSGDVMDVNENTDLGSSEYATASQAKKDAAVPVVNATTPASASGIHKVVIISKLTK
YDKLRKAMNDLGVTGMTVTQVMGCGIQKGAGEKYRGAELDATLLPKVKVEVIVGKIPVEKVIETAKKTLYTGHIGDGKIF
VYDVAQVVKVRTGEEGIEALQDVE

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=412, Percent_Identity=32.2815533980583, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1788904, Length=112, Percent_Identity=50, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1786655, Length=109, Percent_Identity=45.8715596330275, Blast_Score=100, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17550180, Length=424, Percent_Identity=36.7924528301887, Blast_Score=234, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI17550182, Length=425, Percent_Identity=37.4117647058824, Blast_Score=227, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI32564042, Length=427, Percent_Identity=34.192037470726, Blast_Score=210, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI133931100, Length=437, Percent_Identity=32.9519450800915, Blast_Score=198, Evalue=7e-51,
Organism=Saccharomyces cerevisiae, GI6324187, Length=420, Percent_Identity=28.3333333333333, Blast_Score=134, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6325396, Length=423, Percent_Identity=28.6052009456265, Blast_Score=118, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6321559, Length=414, Percent_Identity=27.536231884058, Blast_Score=116, Evalue=1e-26,
Organism=Drosophila melanogaster, GI161078314, Length=547, Percent_Identity=31.9926873857404, Blast_Score=223, Evalue=3e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 61884; Mature: 61753

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS01219 AMMONIUM_TRANSP ; PS00638 PII_GLNB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEEISGVIFGVWFLIGAALVFWMQAGFAMVEAGFTRAKNTGNILMKNLMDFCIGTVVFI
CCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCHHCCCCCHHHHHHHHHHHHHHHHHHH
LIGFSLLLGEDVLGFIGKPGFDIFTSYKDFDWSNFVFNLVFCATTATIVSGAMAERTKFI
HHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYCVYSGVISALIYPIEAHWIWGGGWLSQIGFHDFAGSCAIHMVGGISALVGAAILGPRI
HHHHHHHHHHHHHHEEEEEEEECCHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCC
GKFTKDKNGKITKVNAIPGHNITIGALGVFILWLGWYGFNGAAAKSVEQLGSIFVTTTIA
CCCCCCCCCCEEEEECCCCCCEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
PALATVVCMIFTWLKYGKPDVSMCLNASLAGLVAITAGCDVTDALGAIIIGSVAGLLVCF
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHH
GVWFLDNVLRVDDPVGAVAVHMMNGIWGTIAVGLFATNSAPGYSIADSKGNELVGLFYGG
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCEECCCCCCEEEEEEECC
GFKLLGLQLTGFVSVAAWTVVTITIVFLVIKATLGLRVSEEEEIIGLDPTEHGLPSAYAG
CEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCEEECCCCCCCCCCHHHC
FAIMDVSGDVMDVNENTDLGSSEYATASQAKKDAAVPVVNATTPASASGIHKVVIISKLT
EEEEECCCCEEECCCCCCCCCCCHHHHHHHHCCCCCEEEECCCCCCCCCCEEHHHHHHHH
KYDKLRKAMNDLGVTGMTVTQVMGCGIQKGAGEKYRGAELDATLLPKVKVEVIVGKIPVE
HHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCHH
KVIETAKKTLYTGHIGDGKIFVYDVAQVVKVRTGEEGIEALQDVE
HHHHHHHHHEEEEECCCCCEEEEEHHHHHHHCCCHHHHHHHHCCC
>Mature Secondary Structure 
TEEISGVIFGVWFLIGAALVFWMQAGFAMVEAGFTRAKNTGNILMKNLMDFCIGTVVFI
CCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCHHCCCCCHHHHHHHHHHHHHHHHHHH
LIGFSLLLGEDVLGFIGKPGFDIFTSYKDFDWSNFVFNLVFCATTATIVSGAMAERTKFI
HHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYCVYSGVISALIYPIEAHWIWGGGWLSQIGFHDFAGSCAIHMVGGISALVGAAILGPRI
HHHHHHHHHHHHHHEEEEEEEECCHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHCCCC
GKFTKDKNGKITKVNAIPGHNITIGALGVFILWLGWYGFNGAAAKSVEQLGSIFVTTTIA
CCCCCCCCCCEEEEECCCCCCEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
PALATVVCMIFTWLKYGKPDVSMCLNASLAGLVAITAGCDVTDALGAIIIGSVAGLLVCF
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHH
GVWFLDNVLRVDDPVGAVAVHMMNGIWGTIAVGLFATNSAPGYSIADSKGNELVGLFYGG
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCEECCCCCCEEEEEEECC
GFKLLGLQLTGFVSVAAWTVVTITIVFLVIKATLGLRVSEEEEIIGLDPTEHGLPSAYAG
CEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCEEECCCCCCCCCCHHHC
FAIMDVSGDVMDVNENTDLGSSEYATASQAKKDAAVPVVNATTPASASGIHKVVIISKLT
EEEEECCCCEEECCCCCCCCCCCHHHHHHHHCCCCCEEEECCCCCCCCCCEEHHHHHHHH
KYDKLRKAMNDLGVTGMTVTQVMGCGIQKGAGEKYRGAELDATLLPKVKVEVIVGKIPVE
HHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCHH
KVIETAKKTLYTGHIGDGKIFVYDVAQVVKVRTGEEGIEALQDVE
HHHHHHHHHEEEEECCCCCEEEEEHHHHHHHCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]