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The map label for this gene is metK [H]

Identifier: 238922875

GI number: 238922875

Start: 420240

End: 421421

Strand: Direct

Name: metK [H]

Synonym: EUBREC_0463

Alternate gene names: 238922875

Gene position: 420240-421421 (Clockwise)

Preceding gene: 238922873

Following gene: 238922876

Centisome position: 12.18

GC content: 47.29

Gene sequence:

>1182_bases
ATGGAGAAATTATTATTTACATCAGAATCAGTAACTGAGGGACATCCGGATAAGCTCTGCGATGCAGTATCAGATGCCAT
CCTTGATGCATGCATACAGCAGGACCCGATGAGCCGTGTCGCATGTGAGACAGCAAGCTGTACAGGCTTCATCCTTGTAA
CAGGAGAGATTACCACAAAGGCACAGCTTGATATTCCTGCAATCGTCAGAAAAACAGCACTTGAGATCGGATATGATGAC
GGAAAGAAGGGACTCGACGGAAACTCATGTGCAGTAATGGTTTCAATCGACAAGCAGTCTGCTGATATCGCCATGGGAGT
TGACAAGGCACTCGAGGCAAAAGAGGGCGACCTTACAGACAACCTCGACACCGGAGCCGGAGATCAGGGAATGATGTTTG
GATATGCTACAAATGAGACTGAGGAGTATATGCCATACCCAATCGCACTCGCTCACAAGCTTGCATTCCAGCTCACAAAG
GTTAGAAAAGACGGCACACTTACATACCTTCGTCCGGACGGAAAGACACAGGTTTCAGTAGAGTATGATGAGGCAGGAAA
GCCGGTAAGACTTGAGGCAGTTGTACTTTCAACACAGCACGACGAGGATGTTACCCAGGAGCAGATCCATGAGGACATCA
AGAAATATGTATTTGATCCAATTCTTCCAAAGGATATGATTGACGATGAGACAAAGTTCTTTATCAATCCTACAGGACGT
TTCGTAATAGGTGGACCACACGGAGATGCAGGTCTCACCGGTCGTAAGATTATCGTAGACACCTACGGTGGATATGCACG
TCACGGCGGCGGAGCTTTCTCAGGAAAGGACTGTACAAAGGTAGACCGTTCAGCAGCATACGCAGCACGTTACGTAGCAA
AGAACCTTGTAGCAGCAGGATTTGCTGACAGGTGTGAGATTCAGCTTTCATATGCTATCGGTGTTGCACAGCCAACATCT
ATTATGGTTGATACATTCGGCACAGGAAAGCTTGCAGATGACAGAATCGTTGAAATTGTAAGGGAGAATTTCGACCTTCG
TCCGGCCGGAATCATCAAGATGCTTGACCTCAGAAGACCTATCTACCGCCAGACAGCGGCATACGGACACTTTGGACGTA
CAGATATCAAGCTGCCTTGGGAGGCACTTGATAAGGTTGAGGACCTCAAGAAGTATCTCTAA

Upstream 100 bases:

>100_bases
GGATTTTTATTATCTTAATTTTAATCCACTTATTTGCAGTAAGTGGTTTTTTATTGGAAAAATATTGGTCACACAGTATG
AAAGGAGCAACAGATAATTA

Downstream 100 bases:

>100_bases
ACAATATGTGTGGCTTCGCGCTTTTTAGGTTGTGAGCGCCGGATAGTATACATTATATTTCTTAGAAAAAGTTAGAAAGA
ATTTAGAGAAAATTTAGACA

Product: methionine adenosyltransferase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MEKLLFTSESVTEGHPDKLCDAVSDAILDACIQQDPMSRVACETASCTGFILVTGEITTKAQLDIPAIVRKTALEIGYDD
GKKGLDGNSCAVMVSIDKQSADIAMGVDKALEAKEGDLTDNLDTGAGDQGMMFGYATNETEEYMPYPIALAHKLAFQLTK
VRKDGTLTYLRPDGKTQVSVEYDEAGKPVRLEAVVLSTQHDEDVTQEQIHEDIKKYVFDPILPKDMIDDETKFFINPTGR
FVIGGPHGDAGLTGRKIIVDTYGGYARHGGGAFSGKDCTKVDRSAAYAARYVAKNLVAAGFADRCEIQLSYAIGVAQPTS
IMVDTFGTGKLADDRIVEIVRENFDLRPAGIIKMLDLRRPIYRQTAAYGHFGRTDIKLPWEALDKVEDLKKYL

Sequences:

>Translated_393_residues
MEKLLFTSESVTEGHPDKLCDAVSDAILDACIQQDPMSRVACETASCTGFILVTGEITTKAQLDIPAIVRKTALEIGYDD
GKKGLDGNSCAVMVSIDKQSADIAMGVDKALEAKEGDLTDNLDTGAGDQGMMFGYATNETEEYMPYPIALAHKLAFQLTK
VRKDGTLTYLRPDGKTQVSVEYDEAGKPVRLEAVVLSTQHDEDVTQEQIHEDIKKYVFDPILPKDMIDDETKFFINPTGR
FVIGGPHGDAGLTGRKIIVDTYGGYARHGGGAFSGKDCTKVDRSAAYAARYVAKNLVAAGFADRCEIQLSYAIGVAQPTS
IMVDTFGTGKLADDRIVEIVRENFDLRPAGIIKMLDLRRPIYRQTAAYGHFGRTDIKLPWEALDKVEDLKKYL
>Mature_393_residues
MEKLLFTSESVTEGHPDKLCDAVSDAILDACIQQDPMSRVACETASCTGFILVTGEITTKAQLDIPAIVRKTALEIGYDD
GKKGLDGNSCAVMVSIDKQSADIAMGVDKALEAKEGDLTDNLDTGAGDQGMMFGYATNETEEYMPYPIALAHKLAFQLTK
VRKDGTLTYLRPDGKTQVSVEYDEAGKPVRLEAVVLSTQHDEDVTQEQIHEDIKKYVFDPILPKDMIDDETKFFINPTGR
FVIGGPHGDAGLTGRKIIVDTYGGYARHGGGAFSGKDCTKVDRSAAYAARYVAKNLVAAGFADRCEIQLSYAIGVAQPTS
IMVDTFGTGKLADDRIVEIVRENFDLRPAGIIKMLDLRRPIYRQTAAYGHFGRTDIKLPWEALDKVEDLKKYL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=387, Percent_Identity=55.0387596899225, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI4557737, Length=386, Percent_Identity=54.1450777202073, Blast_Score=407, Evalue=1e-114,
Organism=Escherichia coli, GI1789311, Length=390, Percent_Identity=62.8205128205128, Blast_Score=494, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI25145633, Length=391, Percent_Identity=54.7314578005115, Blast_Score=417, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17538498, Length=385, Percent_Identity=55.3246753246753, Blast_Score=417, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17538494, Length=385, Percent_Identity=54.8051948051948, Blast_Score=412, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17551082, Length=382, Percent_Identity=54.4502617801047, Blast_Score=410, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI32565905, Length=385, Percent_Identity=49.6103896103896, Blast_Score=353, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI32565911, Length=337, Percent_Identity=53.7091988130564, Blast_Score=347, Evalue=9e-96,
Organism=Caenorhabditis elegans, GI25145635, Length=343, Percent_Identity=53.0612244897959, Blast_Score=346, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI32565903, Length=310, Percent_Identity=53.5483870967742, Blast_Score=314, Evalue=5e-86,
Organism=Caenorhabditis elegans, GI32565909, Length=86, Percent_Identity=56.9767441860465, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI32565907, Length=40, Percent_Identity=70, Blast_Score=68, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6320710, Length=389, Percent_Identity=58.3547557840617, Blast_Score=441, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6323209, Length=381, Percent_Identity=59.3175853018373, Blast_Score=439, Evalue=1e-124,
Organism=Drosophila melanogaster, GI45552159, Length=387, Percent_Identity=52.9715762273902, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580529, Length=387, Percent_Identity=52.9715762273902, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580537, Length=387, Percent_Identity=52.9715762273902, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580533, Length=387, Percent_Identity=52.9715762273902, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580535, Length=387, Percent_Identity=52.9715762273902, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580531, Length=387, Percent_Identity=52.9715762273902, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580527, Length=387, Percent_Identity=52.9715762273902, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580525, Length=387, Percent_Identity=52.9715762273902, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580541, Length=374, Percent_Identity=51.8716577540107, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580539, Length=126, Percent_Identity=53.1746031746032, Blast_Score=129, Evalue=3e-30,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 43000; Mature: 43000

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLLFTSESVTEGHPDKLCDAVSDAILDACIQQDPMSRVACETASCTGFILVTGEITTK
CCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEECCCEE
AQLDIPAIVRKTALEIGYDDGKKGLDGNSCAVMVSIDKQSADIAMGVDKALEAKEGDLTD
EECCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCEEECHHHHHHCCCCCCCC
NLDTGAGDQGMMFGYATNETEEYMPYPIALAHKLAFQLTKVRKDGTLTYLRPDGKTQVSV
CCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEE
EYDEAGKPVRLEAVVLSTQHDEDVTQEQIHEDIKKYVFDPILPKDMIDDETKFFINPTGR
EECCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHCCCCCEEEECCCCC
FVIGGPHGDAGLTGRKIIVDTYGGYARHGGGAFSGKDCTKVDRSAAYAARYVAKNLVAAG
EEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
FADRCEIQLSYAIGVAQPTSIMVDTFGTGKLADDRIVEIVRENFDLRPAGIIKMLDLRRP
CCCCEEEEEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCH
IYRQTAAYGHFGRTDIKLPWEALDKVEDLKKYL
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MEKLLFTSESVTEGHPDKLCDAVSDAILDACIQQDPMSRVACETASCTGFILVTGEITTK
CCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEEECCCEE
AQLDIPAIVRKTALEIGYDDGKKGLDGNSCAVMVSIDKQSADIAMGVDKALEAKEGDLTD
EECCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCEEECHHHHHHCCCCCCCC
NLDTGAGDQGMMFGYATNETEEYMPYPIALAHKLAFQLTKVRKDGTLTYLRPDGKTQVSV
CCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEE
EYDEAGKPVRLEAVVLSTQHDEDVTQEQIHEDIKKYVFDPILPKDMIDDETKFFINPTGR
EECCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHCCCCCEEEECCCCC
FVIGGPHGDAGLTGRKIIVDTYGGYARHGGGAFSGKDCTKVDRSAAYAARYVAKNLVAAG
EEEECCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
FADRCEIQLSYAIGVAQPTSIMVDTFGTGKLADDRIVEIVRENFDLRPAGIIKMLDLRRP
CCCCEEEEEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHCCH
IYRQTAAYGHFGRTDIKLPWEALDKVEDLKKYL
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA