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The map label for this gene is tuf

Identifier: 238922795

GI number: 238922795

Start: 351330

End: 352517

Strand: Direct

Name: tuf

Synonym: EUBREC_0383

Alternate gene names: 238922795

Gene position: 351330-352517 (Clockwise)

Preceding gene: 238922794

Following gene: 238922797

Centisome position: 10.18

GC content: 46.21

Gene sequence:

>1188_bases
ATGGCAAAGGCTAAATTTGAAAGAACAAAACCACATTGTAATATTGGTACCATCGGTCACGTAGATCATGGTAAAACAAC
TTTAACAGCAGCTATTACAGCAGTATTAGCAGCTCGTGTCGCTGGTAACACAGCTACAGATTTCGCTAACATTGATAAGG
CTCCAGAGGAGAGAGAGCGTGGAATCACAATTTCTACAGCTCACGTTGAGTATGAGACAGAAAAGAGACATTACGCACAC
GTTGACTGTCCTGGACATGCTGACTACGTAAAGAACATGATCACTGGTGCAGCTCAGATGGATGGTGCTATCCTCGTTGT
TGCTGCTACTGATGGTGTTATGGCTCAGACAAAGGAGCACATCCTCCTTTCTCGTCAGGTAGGTGTTCCTTACATCATCG
TATTCCTTAACAAGTGTGATATGGTTGACGACCCAGAGCTTATCGAGCTCGTAGAGATGGAAGTTACAGAGCAGCTTGAG
GAGTACGGATTCAATGATTGCCCAATCATTCAGGGATCAGCTCTTAAGGCTCTTGAGGATCCAAACGGACCTTGGGGAGA
CAAGATCATGGAGCTTATGGATACTGTTGATTCTTACATCCCAGATCCACAGCGTGATACAGATAAGCCATTCCTTATGC
CAGTAGAGGACGTATTCACAATCACAGGTCGTGGTACTGTTGCAACTGGTAGAGTAGAGCGTGGTACACTTCACCTCAAC
GACGAGTTAGAGATCCTTGGTGTTAAAGAGGACGTTCAGAAGACTGTTGTTACTGGAATCGAGATGTTCCGTAAGCAGTT
AGACGAGGCTCAGGCTGGTGATAACATCGGAGCACTTCTCCGTGGTATTAACCGTGACCAGATCGTTCGTGGACAGGTTC
TTGCTAAGCCTGGTACAGTAACATGTCACCATAAGTTCACAGCTCAGGTTTACGTTCTTACAAAGGACGAGGGTGGACGT
CATACTCCATTCTTCAACAACTATCGTCCACAGTTCTACTTCAGAACAACAGACGTAACAGGTGTTTGCGAGCTTCCAGC
AGGAACAGAGATGTGCATGCCTGGTGATAACGTAGAGATGACAATCGAGCTCATCCATCCAGTAGCTATGGAGCAGGGTC
TTACATTCGCTATCCGTGAGGGTGGACGTACAGTAGGATCTGGACGTGTTGCTACAGTTATCGAGTAA

Upstream 100 bases:

>100_bases
ATTTACTTGAAAAAACTTTGATTATTTCGTATAATCAAAGCAGTCGGTAGATTATAAAAATAGCCCATGGGGCATAAAAG
TTTAAGGAGGACGTTTCAAA

Downstream 100 bases:

>100_bases
TTAATAGAATTACAGTAAATTCAAGGCTTCCAAGGTTTTCCTTGGGAGCCTTTTTATAAACTCAAAAATGTGATATAATT
TAAATGGCTCTAATTTGGCT

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MAKAKFERTKPHCNIGTIGHVDHGKTTLTAAITAVLAARVAGNTATDFANIDKAPEERERGITISTAHVEYETEKRHYAH
VDCPGHADYVKNMITGAAQMDGAILVVAATDGVMAQTKEHILLSRQVGVPYIIVFLNKCDMVDDPELIELVEMEVTEQLE
EYGFNDCPIIQGSALKALEDPNGPWGDKIMELMDTVDSYIPDPQRDTDKPFLMPVEDVFTITGRGTVATGRVERGTLHLN
DELEILGVKEDVQKTVVTGIEMFRKQLDEAQAGDNIGALLRGINRDQIVRGQVLAKPGTVTCHHKFTAQVYVLTKDEGGR
HTPFFNNYRPQFYFRTTDVTGVCELPAGTEMCMPGDNVEMTIELIHPVAMEQGLTFAIREGGRTVGSGRVATVIE

Sequences:

>Translated_395_residues
MAKAKFERTKPHCNIGTIGHVDHGKTTLTAAITAVLAARVAGNTATDFANIDKAPEERERGITISTAHVEYETEKRHYAH
VDCPGHADYVKNMITGAAQMDGAILVVAATDGVMAQTKEHILLSRQVGVPYIIVFLNKCDMVDDPELIELVEMEVTEQLE
EYGFNDCPIIQGSALKALEDPNGPWGDKIMELMDTVDSYIPDPQRDTDKPFLMPVEDVFTITGRGTVATGRVERGTLHLN
DELEILGVKEDVQKTVVTGIEMFRKQLDEAQAGDNIGALLRGINRDQIVRGQVLAKPGTVTCHHKFTAQVYVLTKDEGGR
HTPFFNNYRPQFYFRTTDVTGVCELPAGTEMCMPGDNVEMTIELIHPVAMEQGLTFAIREGGRTVGSGRVATVIE
>Mature_394_residues
AKAKFERTKPHCNIGTIGHVDHGKTTLTAAITAVLAARVAGNTATDFANIDKAPEERERGITISTAHVEYETEKRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVAATDGVMAQTKEHILLSRQVGVPYIIVFLNKCDMVDDPELIELVEMEVTEQLEE
YGFNDCPIIQGSALKALEDPNGPWGDKIMELMDTVDSYIPDPQRDTDKPFLMPVEDVFTITGRGTVATGRVERGTLHLND
ELEILGVKEDVQKTVVTGIEMFRKQLDEAQAGDNIGALLRGINRDQIVRGQVLAKPGTVTCHHKFTAQVYVLTKDEGGRH
TPFFNNYRPQFYFRTTDVTGVCELPAGTEMCMPGDNVEMTIELIHPVAMEQGLTFAIREGGRTVGSGRVATVIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=396, Percent_Identity=53.7878787878788, Blast_Score=435, Evalue=1e-122,
Organism=Homo sapiens, GI4503475, Length=351, Percent_Identity=34.7578347578348, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI4503471, Length=350, Percent_Identity=34.5714285714286, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI223555963, Length=447, Percent_Identity=28.8590604026846, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI5729864, Length=447, Percent_Identity=28.8590604026846, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI194018522, Length=343, Percent_Identity=30.9037900874636, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI194018520, Length=343, Percent_Identity=30.9037900874636, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI194097354, Length=343, Percent_Identity=30.9037900874636, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI46094014, Length=354, Percent_Identity=29.3785310734463, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI54607086, Length=324, Percent_Identity=30.8641975308642, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI53729339, Length=235, Percent_Identity=34.8936170212766, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI53729337, Length=235, Percent_Identity=34.8936170212766, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI157426893, Length=247, Percent_Identity=27.1255060728745, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI94966754, Length=218, Percent_Identity=29.8165137614679, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1790412, Length=396, Percent_Identity=70.959595959596, Blast_Score=551, Evalue=1e-158,
Organism=Escherichia coli, GI1789737, Length=396, Percent_Identity=70.959595959596, Blast_Score=551, Evalue=1e-158,
Organism=Escherichia coli, GI2367247, Length=287, Percent_Identity=33.1010452961672, Blast_Score=138, Evalue=7e-34,
Organism=Escherichia coli, GI1789108, Length=260, Percent_Identity=33.4615384615385, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI48994988, Length=278, Percent_Identity=29.8561151079137, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788922, Length=333, Percent_Identity=24.9249249249249, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1790835, Length=148, Percent_Identity=29.7297297297297, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=404, Percent_Identity=49.009900990099, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI25141371, Length=374, Percent_Identity=44.1176470588235, Blast_Score=308, Evalue=5e-84,
Organism=Caenorhabditis elegans, GI17552884, Length=350, Percent_Identity=34.5714285714286, Blast_Score=155, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17569207, Length=350, Percent_Identity=34.5714285714286, Blast_Score=155, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI32566303, Length=334, Percent_Identity=32.3353293413174, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI32566629, Length=356, Percent_Identity=29.2134831460674, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=273, Percent_Identity=30.4029304029304, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI115532067, Length=263, Percent_Identity=32.6996197718631, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI115532065, Length=263, Percent_Identity=32.6996197718631, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI32566301, Length=149, Percent_Identity=38.255033557047, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17557151, Length=231, Percent_Identity=27.7056277056277, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17556745, Length=133, Percent_Identity=36.8421052631579, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324761, Length=399, Percent_Identity=66.1654135338346, Blast_Score=530, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6325337, Length=354, Percent_Identity=32.7683615819209, Blast_Score=149, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6319594, Length=354, Percent_Identity=32.7683615819209, Blast_Score=149, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6322937, Length=349, Percent_Identity=27.7936962750716, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320377, Length=344, Percent_Identity=27.3255813953488, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323320, Length=253, Percent_Identity=27.6679841897233, Blast_Score=76, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6323098, Length=144, Percent_Identity=36.1111111111111, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322359, Length=134, Percent_Identity=33.5820895522388, Blast_Score=66, Evalue=9e-12,
Organism=Drosophila melanogaster, GI281363316, Length=395, Percent_Identity=54.9367088607595, Blast_Score=441, Evalue=1e-124,
Organism=Drosophila melanogaster, GI17864358, Length=395, Percent_Identity=54.9367088607595, Blast_Score=441, Evalue=1e-124,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=47.9274611398964, Blast_Score=376, Evalue=1e-104,
Organism=Drosophila melanogaster, GI45553807, Length=443, Percent_Identity=30.9255079006772, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI45553816, Length=443, Percent_Identity=30.9255079006772, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24651721, Length=443, Percent_Identity=30.9255079006772, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI17864154, Length=443, Percent_Identity=30.9255079006772, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24652838, Length=350, Percent_Identity=34.5714285714286, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI17137572, Length=350, Percent_Identity=34.5714285714286, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI19922690, Length=299, Percent_Identity=32.1070234113712, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI45550900, Length=337, Percent_Identity=31.4540059347181, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI17137380, Length=344, Percent_Identity=28.7790697674419, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24647046, Length=279, Percent_Identity=28.3154121863799, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24647048, Length=279, Percent_Identity=28.3154121863799, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI78706572, Length=245, Percent_Identity=28.9795918367347, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI28572034, Length=168, Percent_Identity=40.4761904761905, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24582462, Length=150, Percent_Identity=36, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_EUBR3 (C4ZB99)

Other databases:

- EMBL:   CP001107
- RefSeq:   YP_002936308.1
- GeneID:   7966471
- GenomeReviews:   CP001107_GR
- KEGG:   ere:EUBREC_0383
- ProtClustDB:   PRK00049
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 43411; Mature: 43280

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAKFERTKPHCNIGTIGHVDHGKTTLTAAITAVLAARVAGNTATDFANIDKAPEERER
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCHHHHC
GITISTAHVEYETEKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVAATDGVMAQTKEH
CCEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEEECCCHHHHHHHH
ILLSRQVGVPYIIVFLNKCDMVDDPELIELVEMEVTEQLEEYGFNDCPIIQGSALKALED
HEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHHCC
PNGPWGDKIMELMDTVDSYIPDPQRDTDKPFLMPVEDVFTITGRGTVATGRVERGTLHLN
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHEEEEECCCEEEECCEECCEEEEC
DELEILGVKEDVQKTVVTGIEMFRKQLDEAQAGDNIGALLRGINRDQIVRGQVLAKPGTV
CCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHEEECEEEECCCEE
TCHHKFTAQVYVLTKDEGGRHTPFFNNYRPQFYFRTTDVTGVCELPAGTEMCMPGDNVEM
EEEEEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCCEECCCCCCEE
TIELIHPVAMEQGLTFAIREGGRTVGSGRVATVIE
EEEEHHHHHHHCCCEEEEECCCCEECCCEEEEEEC
>Mature Secondary Structure 
AKAKFERTKPHCNIGTIGHVDHGKTTLTAAITAVLAARVAGNTATDFANIDKAPEERER
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCHHHHC
GITISTAHVEYETEKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVAATDGVMAQTKEH
CCEEEEEEEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCCEEEEEEECCCHHHHHHHH
ILLSRQVGVPYIIVFLNKCDMVDDPELIELVEMEVTEQLEEYGFNDCPIIQGSALKALED
HEEECCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCHHHHCC
PNGPWGDKIMELMDTVDSYIPDPQRDTDKPFLMPVEDVFTITGRGTVATGRVERGTLHLN
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHEEEEECCCEEEECCEECCEEEEC
DELEILGVKEDVQKTVVTGIEMFRKQLDEAQAGDNIGALLRGINRDQIVRGQVLAKPGTV
CCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHEEECEEEECCCEE
TCHHKFTAQVYVLTKDEGGRHTPFFNNYRPQFYFRTTDVTGVCELPAGTEMCMPGDNVEM
EEEEEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCCEECCCCCCEE
TIELIHPVAMEQGLTFAIREGGRTVGSGRVATVIE
EEEEHHHHHHHCCCEEEEECCCCEECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA