| Definition | Eubacterium rectale ATCC 33656, complete genome. |
|---|---|
| Accession | NC_012781 |
| Length | 3,449,685 |
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The map label for this gene is scrFIBM [H]
Identifier: 238922584
GI number: 238922584
Start: 153687
End: 154988
Strand: Direct
Name: scrFIBM [H]
Synonym: EUBREC_0158
Alternate gene names: 238922584
Gene position: 153687-154988 (Clockwise)
Preceding gene: 238922583
Following gene: 238922585
Centisome position: 4.46
GC content: 34.1
Gene sequence:
>1302_bases ATGGCTTGGAATATACTGTATGGTGATCCCGGGAGAAAAGAAATGTTTAGAGTAATTGAAACTTTTAGTGGAATTGGAAG TCAGGCAAAAGCATTAACAAGAATTGGAAAACCTTTTGAAATTGTAAATACTGCAGATTGGGATATTAACGCAATACTGG CATATTGTCTGATACATAAAGGCAAAATTGATATTAATAAATATGCAGAAATTTCAGATGAAGATGTTACAAATTTTTTA AAAGGTTTATCGCTGAGTTCCGATGGAAAGAAACCTATGAGTGATGAGACGTTTAGGCGAATGCCAATGCATTTGAAACG AAGATTGTATGCAGCAATAAAAGAAACAAGAAATATGGTAAGTATTACGGATGTTATGGGAAGTGATATACCCGACAATA TTGATTTGTTCACATATTCATTTCCTTGTCAGGATTTATCATTATGTGGATGCTGGCATGGGAATAAAAGTGGAATAGCA AGGGATGCACATAATCGTTCTGGTATGCTTTGGGAAGTTGAACGTATATTGCTTGAAATGAATGAAATGGGAAAAGAATT ACCTCGTTTTCTGCTTATGGAAAACGTAACCAATATTCTGTCAAAACCGCATGCTGCAGATTTTGGTGATTGGAAGGCAA CTTTAGAAAATCTGGGATATTATAATAAAATATATAGGCTTAATGCTCAAAATTTTGGTATTCCTCAAAAAAGAGAAAGA GCATATATGATCAGTATATTGTGCAAAAATAATGCCGAAACGATAGCCCAAGTAGAAAAGTACTTTGAAGAACATAATTT GGAAAAGGATGAAGCAACGCGTTTAAAGCAAAGGAATTTAAGGTTAAAAGACATTCTTTGTTTAGATTACGATGATGTTC CCAGATATAAGGAAGAAGCTGATGCAAGTAAACCTAATGATACGCCTTCCAGAAGAAAAATATGGGAAGATAATGAATTG CTGTATGATGGAAAAGAGATTAGGGACATCGTTGTTAATACAGTAACGACAAAGCAGGACCGTAATCCTAATTCCGGCTT GATTATTTATAAGAATCGGGCAAGAGGAAAGACAAAATGGAGATATCTGACTCCAAGAGAGTGCTTTAAGTTGATGGGAT TTGATGAATCGGATTTTGACAGGATAATCAGTGATAATCCTATGGTAACAAAGAATAGATATTTGTATTCAACAGAGAAG CTTATTAAACTAGCAGGTAATAGTATCGTGGTTGATGTTTTGGAAGCTATTTTCAGACAGATAATGGATATTAATGAGAA GATTTTAGGTGAAAGAAAATGA
Upstream 100 bases:
>100_bases TATGAGGTTGATGATAAATTTCCGAAGATAACAAAAGCCTCATTTAAGGATGATCATATACCGGAGTCAATAACGCAAAT TACATATACCATTGATTTGG
Downstream 100 bases:
>100_bases CAATAAATCAGAAAAAGTCTTTAGTGGCATTTTACATTTCAAAGTTTAATGATGACGCATTTCGTGAATTGGGATATGGT GGACCGGTGTCAAAAGCGAT
Product: modification methylase, putative
Products: NA
Alternate protein names: M.ScrFI-B; M.ScrFIB; Cytosine-specific methyltransferase ScrFIB [H]
Number of amino acids: Translated: 433; Mature: 432
Protein sequence:
>433_residues MAWNILYGDPGRKEMFRVIETFSGIGSQAKALTRIGKPFEIVNTADWDINAILAYCLIHKGKIDINKYAEISDEDVTNFL KGLSLSSDGKKPMSDETFRRMPMHLKRRLYAAIKETRNMVSITDVMGSDIPDNIDLFTYSFPCQDLSLCGCWHGNKSGIA RDAHNRSGMLWEVERILLEMNEMGKELPRFLLMENVTNILSKPHAADFGDWKATLENLGYYNKIYRLNAQNFGIPQKRER AYMISILCKNNAETIAQVEKYFEEHNLEKDEATRLKQRNLRLKDILCLDYDDVPRYKEEADASKPNDTPSRRKIWEDNEL LYDGKEIRDIVVNTVTTKQDRNPNSGLIIYKNRARGKTKWRYLTPRECFKLMGFDESDFDRIISDNPMVTKNRYLYSTEK LIKLAGNSIVVDVLEAIFRQIMDINEKILGERK
Sequences:
>Translated_433_residues MAWNILYGDPGRKEMFRVIETFSGIGSQAKALTRIGKPFEIVNTADWDINAILAYCLIHKGKIDINKYAEISDEDVTNFL KGLSLSSDGKKPMSDETFRRMPMHLKRRLYAAIKETRNMVSITDVMGSDIPDNIDLFTYSFPCQDLSLCGCWHGNKSGIA RDAHNRSGMLWEVERILLEMNEMGKELPRFLLMENVTNILSKPHAADFGDWKATLENLGYYNKIYRLNAQNFGIPQKRER AYMISILCKNNAETIAQVEKYFEEHNLEKDEATRLKQRNLRLKDILCLDYDDVPRYKEEADASKPNDTPSRRKIWEDNEL LYDGKEIRDIVVNTVTTKQDRNPNSGLIIYKNRARGKTKWRYLTPRECFKLMGFDESDFDRIISDNPMVTKNRYLYSTEK LIKLAGNSIVVDVLEAIFRQIMDINEKILGERK >Mature_432_residues AWNILYGDPGRKEMFRVIETFSGIGSQAKALTRIGKPFEIVNTADWDINAILAYCLIHKGKIDINKYAEISDEDVTNFLK GLSLSSDGKKPMSDETFRRMPMHLKRRLYAAIKETRNMVSITDVMGSDIPDNIDLFTYSFPCQDLSLCGCWHGNKSGIAR DAHNRSGMLWEVERILLEMNEMGKELPRFLLMENVTNILSKPHAADFGDWKATLENLGYYNKIYRLNAQNFGIPQKRERA YMISILCKNNAETIAQVEKYFEEHNLEKDEATRLKQRNLRLKDILCLDYDDVPRYKEEADASKPNDTPSRRKIWEDNELL YDGKEIRDIVVNTVTTKQDRNPNSGLIIYKNRARGKTKWRYLTPRECFKLMGFDESDFDRIISDNPMVTKNRYLYSTEKL IKLAGNSIVVDVLEAIFRQIMDINEKILGERK
Specific function: This methylase recognizes the double-stranded sequence CCNGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the ScrFI endonuclease [H]
COG id: COG0270
COG function: function code L; Site-specific DNA methylase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the C5-methyltransferase family [H]
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR018117 - InterPro: IPR001525 [H]
Pfam domain/function: PF00145 DNA_methylase [H]
EC number: =2.1.1.37 [H]
Molecular weight: Translated: 50249; Mature: 50118
Theoretical pI: Translated: 8.03; Mature: 8.03
Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.6 %Cys (Translated Protein) 3.5 %Met (Translated Protein) 5.1 %Cys+Met (Translated Protein) 1.6 %Cys (Mature Protein) 3.2 %Met (Mature Protein) 4.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MAWNILYGDPGRKEMFRVIETFSGIGSQAKALTRIGKPFEIVNTADWDINAILAYCLIHK CEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHC GKIDINKYAEISDEDVTNFLKGLSLSSDGKKPMSDETFRRMPMHLKRRLYAAIKETRNMV CCCCHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCE SITDVMGSDIPDNIDLFTYSFPCQDLSLCGCWHGNKSGIARDAHNRSGMLWEVERILLEM EHHHHHCCCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHH NEMGKELPRFLLMENVTNILSKPHAADFGDWKATLENLGYYNKIYRLNAQNFGIPQKRER HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHEEEECCCCCCCCCHHCC AYMISILCKNNAETIAQVEKYFEEHNLEKDEATRLKQRNLRLKDILCLDYDDVPRYKEEA EEEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHEEEECCCCCCCHHHHH DASKPNDTPSRRKIWEDNELLYDGKEIRDIVVNTVTTKQDRNPNSGLIIYKNRARGKTKW CCCCCCCCCHHHCCCCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCE RYLTPRECFKLMGFDESDFDRIISDNPMVTKNRYLYSTEKLIKLAGNSIVVDVLEAIFRQ EECCHHHHHHHHCCCCHHHHHHHCCCCCEECCCEEHHHHHHHHHHCCHHHHHHHHHHHHH IMDINEKILGERK HHHHHHHHCCCCC >Mature Secondary Structure AWNILYGDPGRKEMFRVIETFSGIGSQAKALTRIGKPFEIVNTADWDINAILAYCLIHK EEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHC GKIDINKYAEISDEDVTNFLKGLSLSSDGKKPMSDETFRRMPMHLKRRLYAAIKETRNMV CCCCHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCE SITDVMGSDIPDNIDLFTYSFPCQDLSLCGCWHGNKSGIARDAHNRSGMLWEVERILLEM EHHHHHCCCCCCCCEEEEEECCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHH NEMGKELPRFLLMENVTNILSKPHAADFGDWKATLENLGYYNKIYRLNAQNFGIPQKRER HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHEEEECCCCCCCCCHHCC AYMISILCKNNAETIAQVEKYFEEHNLEKDEATRLKQRNLRLKDILCLDYDDVPRYKEEA EEEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHEEEECCCCCCCHHHHH DASKPNDTPSRRKIWEDNELLYDGKEIRDIVVNTVTTKQDRNPNSGLIIYKNRARGKTKW CCCCCCCCCHHHCCCCCCCEEECCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCE RYLTPRECFKLMGFDESDFDRIISDNPMVTKNRYLYSTEKLIKLAGNSIVVDVLEAIFRQ EECCHHHHHHHHCCCCHHHHHHHCCCCCEECCCEEHHHHHHHHHHCCHHHHHHHHHHHHH IMDINEKILGERK HHHHHHHHCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA