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The map label for this gene is dus1 [H]

Identifier: 238922487

GI number: 238922487

Start: 65070

End: 66041

Strand: Direct

Name: dus1 [H]

Synonym: EUBREC_0061

Alternate gene names: 238922487

Gene position: 65070-66041 (Clockwise)

Preceding gene: 238922486

Following gene: 238922488

Centisome position: 1.89

GC content: 48.77

Gene sequence:

>972_bases
ATGGATATTCATCAGATACGAAAGCTTCAGATAGGAAGTGTGACACTCCCAAACAACCTGATACTGGGACCTATGGCAGG
CGTGACAGACCTGCCATTTCGCTTATTGTGCAAGGAGCAGGGAGCCGGGCTTTTATGTATGGAAATGGTCAGCGCCAAGG
CAATTTTGTACAAAAACAAAAATACAGAGTCGCTTCTTTCAATAGATGAAAGAGAAAAGCCTGTTTCACTCCAGATGTTT
GGCTCAGACCCGCAGATAATGGCTCAGATAGCAAAGCAGATAGAGGAAAGACCGTTTGACATACTGGATATCAACATGGG
TTGTCCGGTGCCCAAAATCGTAAACAACGGTGAGGGCTCAGCACTCATGAAAAATCCGCTTCTTGCAGGTCAGATTATAG
AGAGCATAGTAAAAGCGATAGATAAGCCGGTCACCGTAAAAATACGAAAGGGCTTCGATGATGAGCATGTCAACGCAGTA
GAGCTGGCACATGTGGCTGAAGAAAGCGGCGCAAGCGCAGTCGCAGTGCATGGCAGGACCAGAGAGCAGTACTACTCCGG
TAAAGCCGACTGGGACATCATAAGGCAGGTAAAAGAGGCTGTCTCAATACCGGTCATAGGAAACGGAGACCTGCTTAGCG
CAGAAGATGTCATAAAAATGCAGGAGCAGACAGGCTGCGACGGCTTTATGATAGCCAGGGGAGCGCAGGGCAATCCATGG
ATTTTCTCACAGATACTGCATTACTTTGAGACCGGCGAGCATCAGGAAAAGCCATCCTTTGAGGAAGTACGCCGGATGAT
ACTGCGCCATGCACGCATGATGATAGAGTTCAAGGGCGAGTACACGGGAATACACGAGATGAGAAAGCACACAGCGTGGT
ATACGGCAGGATATCCGCATTCATCAAGGCTGCGCGCGGCGGTGAATACGGTGGAGAGCTTAGAACAGCTTGAGGAGCTT
TTGTATAAGTAG

Upstream 100 bases:

>100_bases
AATGAGACAGAAAACGTGGATGTATACGATCCGGATCTGACATTAAAGGGCATAAACCTCGTGTATCGCAAGCAGAACCG
CAAGGGAGTGAAGTAATAGT

Downstream 100 bases:

>100_bases
AAGATTCGAAATTGAAATCGGCCAAATCCTTGACAGCGTGGGGATTTTCAATTATAATACACGCGTTAAAATAGGCTTTA
CGATTTAATAAAACAAACTT

Product: tRNA-dihydrouridine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MDIHQIRKLQIGSVTLPNNLILGPMAGVTDLPFRLLCKEQGAGLLCMEMVSAKAILYKNKNTESLLSIDEREKPVSLQMF
GSDPQIMAQIAKQIEERPFDILDINMGCPVPKIVNNGEGSALMKNPLLAGQIIESIVKAIDKPVTVKIRKGFDDEHVNAV
ELAHVAEESGASAVAVHGRTREQYYSGKADWDIIRQVKEAVSIPVIGNGDLLSAEDVIKMQEQTGCDGFMIARGAQGNPW
IFSQILHYFETGEHQEKPSFEEVRRMILRHARMMIEFKGEYTGIHEMRKHTAWYTAGYPHSSRLRAAVNTVESLEQLEEL
LYK

Sequences:

>Translated_323_residues
MDIHQIRKLQIGSVTLPNNLILGPMAGVTDLPFRLLCKEQGAGLLCMEMVSAKAILYKNKNTESLLSIDEREKPVSLQMF
GSDPQIMAQIAKQIEERPFDILDINMGCPVPKIVNNGEGSALMKNPLLAGQIIESIVKAIDKPVTVKIRKGFDDEHVNAV
ELAHVAEESGASAVAVHGRTREQYYSGKADWDIIRQVKEAVSIPVIGNGDLLSAEDVIKMQEQTGCDGFMIARGAQGNPW
IFSQILHYFETGEHQEKPSFEEVRRMILRHARMMIEFKGEYTGIHEMRKHTAWYTAGYPHSSRLRAAVNTVESLEQLEEL
LYK
>Mature_323_residues
MDIHQIRKLQIGSVTLPNNLILGPMAGVTDLPFRLLCKEQGAGLLCMEMVSAKAILYKNKNTESLLSIDEREKPVSLQMF
GSDPQIMAQIAKQIEERPFDILDINMGCPVPKIVNNGEGSALMKNPLLAGQIIESIVKAIDKPVTVKIRKGFDDEHVNAV
ELAHVAEESGASAVAVHGRTREQYYSGKADWDIIRQVKEAVSIPVIGNGDLLSAEDVIKMQEQTGCDGFMIARGAQGNPW
IFSQILHYFETGEHQEKPSFEEVRRMILRHARMMIEFKGEYTGIHEMRKHTAWYTAGYPHSSRLRAAVNTVESLEQLEEL
LYK

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788462, Length=246, Percent_Identity=35.3658536585366, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI239788483, Length=229, Percent_Identity=37.117903930131, Blast_Score=151, Evalue=7e-37,
Organism=Homo sapiens, GI8923374, Length=251, Percent_Identity=33.4661354581673, Blast_Score=118, Evalue=7e-27,
Organism=Homo sapiens, GI31742496, Length=227, Percent_Identity=30.3964757709251, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI40807366, Length=229, Percent_Identity=31.8777292576419, Blast_Score=107, Evalue=1e-23,
Organism=Escherichia coli, GI1789660, Length=314, Percent_Identity=42.3566878980892, Blast_Score=248, Evalue=3e-67,
Organism=Escherichia coli, GI1788462, Length=286, Percent_Identity=29.7202797202797, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI145693211, Length=242, Percent_Identity=29.7520661157025, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17543114, Length=247, Percent_Identity=35.6275303643725, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI25144369, Length=222, Percent_Identity=32.4324324324324, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17510279, Length=246, Percent_Identity=34.9593495934959, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17507177, Length=252, Percent_Identity=28.5714285714286, Blast_Score=98, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6323560, Length=308, Percent_Identity=28.2467532467532, Blast_Score=113, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6323433, Length=299, Percent_Identity=30.1003344481605, Blast_Score=105, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6323437, Length=228, Percent_Identity=28.5087719298246, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324342, Length=280, Percent_Identity=26.4285714285714, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24585320, Length=238, Percent_Identity=38.2352941176471, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24580595, Length=227, Percent_Identity=32.15859030837, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI19920448, Length=227, Percent_Identity=32.15859030837, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI45549423, Length=244, Percent_Identity=33.6065573770492, Blast_Score=117, Evalue=9e-27,
Organism=Drosophila melanogaster, GI19921524, Length=228, Percent_Identity=30.2631578947368, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36156; Mature: 36156

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIHQIRKLQIGSVTLPNNLILGPMAGVTDLPFRLLCKEQGAGLLCMEMVSAKAILYKNK
CCHHHHHHEEECCEECCCCEEECCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHEEEECC
NTESLLSIDEREKPVSLQMFGSDPQIMAQIAKQIEERPFDILDINMGCPVPKIVNNGEGS
CHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHCCCCCC
ALMKNPLLAGQIIESIVKAIDKPVTVKIRKGFDDEHVNAVELAHVAEESGASAVAVHGRT
CHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEECCCC
REQYYSGKADWDIIRQVKEAVSIPVIGNGDLLSAEDVIKMQEQTGCDGFMIARGAQGNPW
HHHHHCCCCCHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCH
IFSQILHYFETGEHQEKPSFEEVRRMILRHARMMIEFKGEYTGIHEMRKHTAWYTAGYPH
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHCCEEECCCCC
SSRLRAAVNTVESLEQLEELLYK
HHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDIHQIRKLQIGSVTLPNNLILGPMAGVTDLPFRLLCKEQGAGLLCMEMVSAKAILYKNK
CCHHHHHHEEECCEECCCCEEECCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHEEEECC
NTESLLSIDEREKPVSLQMFGSDPQIMAQIAKQIEERPFDILDINMGCPVPKIVNNGEGS
CHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHCCCCCC
ALMKNPLLAGQIIESIVKAIDKPVTVKIRKGFDDEHVNAVELAHVAEESGASAVAVHGRT
CHHHCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEECCCC
REQYYSGKADWDIIRQVKEAVSIPVIGNGDLLSAEDVIKMQEQTGCDGFMIARGAQGNPW
HHHHHCCCCCHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCH
IFSQILHYFETGEHQEKPSFEEVRRMILRHARMMIEFKGEYTGIHEMRKHTAWYTAGYPH
HHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHCCEEECCCCC
SSRLRAAVNTVESLEQLEELLYK
HHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]