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The map label for this gene is ptsI [H]

Identifier: 238922279

GI number: 238922279

Start: 475498

End: 477123

Strand: Reverse

Name: ptsI [H]

Synonym: EUBELI_20515

Alternate gene names: 238922279

Gene position: 477123-475498 (Counterclockwise)

Preceding gene: 238922280

Following gene: 238922278

Centisome position: 76.13

GC content: 41.02

Gene sequence:

>1626_bases
ATGAATAAATATACTGGAAAAAGTGTATTTGGAGGCATTGCTATAGGTAAGATAATGGTGTATGAAAAAGGTGAACATCA
GGTTAAGCGTGTAAAGATTACTGATGCAGAAGCAGAGAAGAACCGTTATTATGAAGCTGTAGAAACAGCCTTTAAACAAC
TGGGGGAACTTCATGATAAAGCACTTCGCGAAGTTGGCGAGGCTAATGCGGCAATATTTGAGATTCATCAGATGATGCTT
GAGGATGACGATTATAAGGAATCTGTTGAGCATATTATCGAGAGTCAGATGGTGAATGCAGAATATGCCATAGCCCAGAC
AGGGGATAATTTTTCACAGATGTTTGCGGCTATGGACGATGAATATATGCGTGGAAGAGCAGCAGATGTCAAAGATATTA
CAGAAAGACTGTTAGGAATACTTTCCGGCAATACAGGAAGCGGTGTAGATGCAGATGAGCCTGTTATTATGGTGGCAGAG
GATCTGGCACCATCAGAGACAGTCCAGATGGATAAGAGCAAGATATTATCATTTGTTACACAGAAGGGTTCAGTTAATTC
ACATACTGCAATTCTTGCAAGAACAATGGGGATTCCGGCTTTAATTGGTTCAGATATTGTTATTGATGAATCTCTTAATG
GAAAGCTTGCTGTTGTCGATGGAACTAACGGTGTTGTATACATTGAACCTGATGAGGCAACCCTGTCAGTAATGCAGGAG
GAACAGCGCAAAGACAATGAGAAGAAAGCTCTTTTACAGGAACTTAAAGGCAAGGAAGATGTGACTTTAGATGGCAAGAA
GATTAAGCTTTATTCTAATATTGGTAATATTAAGGATCTCGCAAATGTTATTGCCAATGATGCAGCAGGAATCGGACTGT
TTCGAAGTGAATTCATATATCTTGAATCTGATACATTTCCAACAGAAGAAGAACAGTTCAACATATACCGTACTGTAGCT
GAAAGCATGGCCGGAAAACCGGTAATTATAAGGACTCTTGATATCGGTGCTGATAAGCAGTGTGATTATTTCGAATTGGA
TAAAGAGGATAATCCTGCTCTTGGTCTTAGAGCAATCAGGATATGCCTGACAAGACCTGAGATATTCAAGACACAGTTAC
GTGCTCTTTACAGAGCAAGCGCGTATGGAAACATATCTATCATGTACCCTATGATAATCAGCGAACAGGAAGTTGATAAG
ATTAAGGTTATTGAGAATGAAGTCAAGGCTGAGCTTACAGAGCAGGGAATTGAATTCGGCAATCCTAAGAGTGGAATTAT
GATTGAGACTCCGGCAGCAGTAATTATGAGCCGTCAGCTTGCTAAGAAGGTGGATTTCTTCAGTATTGGAACTAATGACC
TGACACAGTACACGCTTGCGATTGACCGTCAGAATACAAAGCTTGATGATTTCTACGATGCTCATCATCCAGCCATTCTT
GCCATGATAAGAATGGTTGTTGAGAATGCACATGCAGAGGGCATCTGGGCTGGTATATGTGGTGAACTCGGTGCAGACAC
AACACTTACAGAAGAGTTTCTTAAGATGGGTGTCGATGAGCTTTCAGTTTCAGCAGGCAAGGTGCTGGCTGTAAGAAAGG
TGATTCGTGAGACGAGGATTTCTTAG

Upstream 100 bases:

>100_bases
AATGAAGTTACAATTACTGTAGAAGGTGAAGATGAAGAGGCTGCAGCAGCTAAGATTGAAGAATTCATGAAGAGCAATAT
GTAGCAGTTAGGAGGTGCAC

Downstream 100 bases:

>100_bases
GAAAACTGCTGTAAGGGTACGTCGTATGGTATGAGGCAGGATGTATGTAGAATCTGCGTCGGGAGAGCTTTTTCGAGTGG
GCATTGTTGAGAGCTCTTTG

Product: phosphotransferase system, enzyme I

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MNKYTGKSVFGGIAIGKIMVYEKGEHQVKRVKITDAEAEKNRYYEAVETAFKQLGELHDKALREVGEANAAIFEIHQMML
EDDDYKESVEHIIESQMVNAEYAIAQTGDNFSQMFAAMDDEYMRGRAADVKDITERLLGILSGNTGSGVDADEPVIMVAE
DLAPSETVQMDKSKILSFVTQKGSVNSHTAILARTMGIPALIGSDIVIDESLNGKLAVVDGTNGVVYIEPDEATLSVMQE
EQRKDNEKKALLQELKGKEDVTLDGKKIKLYSNIGNIKDLANVIANDAAGIGLFRSEFIYLESDTFPTEEEQFNIYRTVA
ESMAGKPVIIRTLDIGADKQCDYFELDKEDNPALGLRAIRICLTRPEIFKTQLRALYRASAYGNISIMYPMIISEQEVDK
IKVIENEVKAELTEQGIEFGNPKSGIMIETPAAVIMSRQLAKKVDFFSIGTNDLTQYTLAIDRQNTKLDDFYDAHHPAIL
AMIRMVVENAHAEGIWAGICGELGADTTLTEEFLKMGVDELSVSAGKVLAVRKVIRETRIS

Sequences:

>Translated_541_residues
MNKYTGKSVFGGIAIGKIMVYEKGEHQVKRVKITDAEAEKNRYYEAVETAFKQLGELHDKALREVGEANAAIFEIHQMML
EDDDYKESVEHIIESQMVNAEYAIAQTGDNFSQMFAAMDDEYMRGRAADVKDITERLLGILSGNTGSGVDADEPVIMVAE
DLAPSETVQMDKSKILSFVTQKGSVNSHTAILARTMGIPALIGSDIVIDESLNGKLAVVDGTNGVVYIEPDEATLSVMQE
EQRKDNEKKALLQELKGKEDVTLDGKKIKLYSNIGNIKDLANVIANDAAGIGLFRSEFIYLESDTFPTEEEQFNIYRTVA
ESMAGKPVIIRTLDIGADKQCDYFELDKEDNPALGLRAIRICLTRPEIFKTQLRALYRASAYGNISIMYPMIISEQEVDK
IKVIENEVKAELTEQGIEFGNPKSGIMIETPAAVIMSRQLAKKVDFFSIGTNDLTQYTLAIDRQNTKLDDFYDAHHPAIL
AMIRMVVENAHAEGIWAGICGELGADTTLTEEFLKMGVDELSVSAGKVLAVRKVIRETRIS
>Mature_541_residues
MNKYTGKSVFGGIAIGKIMVYEKGEHQVKRVKITDAEAEKNRYYEAVETAFKQLGELHDKALREVGEANAAIFEIHQMML
EDDDYKESVEHIIESQMVNAEYAIAQTGDNFSQMFAAMDDEYMRGRAADVKDITERLLGILSGNTGSGVDADEPVIMVAE
DLAPSETVQMDKSKILSFVTQKGSVNSHTAILARTMGIPALIGSDIVIDESLNGKLAVVDGTNGVVYIEPDEATLSVMQE
EQRKDNEKKALLQELKGKEDVTLDGKKIKLYSNIGNIKDLANVIANDAAGIGLFRSEFIYLESDTFPTEEEQFNIYRTVA
ESMAGKPVIIRTLDIGADKQCDYFELDKEDNPALGLRAIRICLTRPEIFKTQLRALYRASAYGNISIMYPMIISEQEVDK
IKVIENEVKAELTEQGIEFGNPKSGIMIETPAAVIMSRQLAKKVDFFSIGTNDLTQYTLAIDRQNTKLDDFYDAHHPAIL
AMIRMVVENAHAEGIWAGICGELGADTTLTEEFLKMGVDELSVSAGKVLAVRKVIRETRIS

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=531, Percent_Identity=41.4312617702448, Blast_Score=403, Evalue=1e-113,
Organism=Escherichia coli, GI1788726, Length=538, Percent_Identity=32.3420074349442, Blast_Score=306, Evalue=3e-84,
Organism=Escherichia coli, GI48994992, Length=473, Percent_Identity=35.5179704016913, Blast_Score=304, Evalue=1e-83,
Organism=Escherichia coli, GI1789193, Length=518, Percent_Identity=34.5559845559846, Blast_Score=301, Evalue=6e-83,
Organism=Escherichia coli, GI1787994, Length=405, Percent_Identity=25.9259259259259, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI226510935, Length=183, Percent_Identity=29.5081967213115, Blast_Score=90, Evalue=4e-19,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 59944; Mature: 59944

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKYTGKSVFGGIAIGKIMVYEKGEHQVKRVKITDAEAEKNRYYEAVETAFKQLGELHDK
CCCCCCCHHHCCCEEEEEEEEECCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
ALREVGEANAAIFEIHQMMLEDDDYKESVEHIIESQMVNAEYAIAQTGDNFSQMFAAMDD
HHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCH
EYMRGRAADVKDITERLLGILSGNTGSGVDADEPVIMVAEDLAPSETVQMDKSKILSFVT
HHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHH
QKGSVNSHTAILARTMGIPALIGSDIVIDESLNGKLAVVDGTNGVVYIEPDEATLSVMQE
CCCCCCCHHHHHHHHCCCCHHHCCCEEEECCCCCEEEEEECCCCEEEECCCHHHHHHHHH
EQRKDNEKKALLQELKGKEDVTLDGKKIKLYSNIGNIKDLANVIANDAAGIGLFRSEFIY
HHHCCHHHHHHHHHHCCCCCEEECCCEEEEEECCCCHHHHHHHHHCCCCCCCEEEEEEEE
LESDTFPTEEEQFNIYRTVAESMAGKPVIIRTLDIGADKQCDYFELDKEDNPALGLRAIR
EECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEECCCCCCCCHHHHHHH
ICLTRPEIFKTQLRALYRASAYGNISIMYPMIISEQEVDKIKVIENEVKAELTEQGIEFG
HHHCCCHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCC
NPKSGIMIETPAAVIMSRQLAKKVDFFSIGTNDLTQYTLAIDRQNTKLDDFYDAHHPAIL
CCCCCEEEECCHHHHHHHHHHHHCCEEECCCCCCEEEEEEEECCCCCCHHHHHCCCHHHH
AMIRMVVENAHAEGIWAGICGELGADTTLTEEFLKMGVDELSVSAGKVLAVRKVIRETRI
HHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHCC
S
C
>Mature Secondary Structure
MNKYTGKSVFGGIAIGKIMVYEKGEHQVKRVKITDAEAEKNRYYEAVETAFKQLGELHDK
CCCCCCCHHHCCCEEEEEEEEECCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
ALREVGEANAAIFEIHQMMLEDDDYKESVEHIIESQMVNAEYAIAQTGDNFSQMFAAMDD
HHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCH
EYMRGRAADVKDITERLLGILSGNTGSGVDADEPVIMVAEDLAPSETVQMDKSKILSFVT
HHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHH
QKGSVNSHTAILARTMGIPALIGSDIVIDESLNGKLAVVDGTNGVVYIEPDEATLSVMQE
CCCCCCCHHHHHHHHCCCCHHHCCCEEEECCCCCEEEEEECCCCEEEECCCHHHHHHHHH
EQRKDNEKKALLQELKGKEDVTLDGKKIKLYSNIGNIKDLANVIANDAAGIGLFRSEFIY
HHHCCHHHHHHHHHHCCCCCEEECCCEEEEEECCCCHHHHHHHHHCCCCCCCEEEEEEEE
LESDTFPTEEEQFNIYRTVAESMAGKPVIIRTLDIGADKQCDYFELDKEDNPALGLRAIR
EECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEECCCCCCCCHHHHHHH
ICLTRPEIFKTQLRALYRASAYGNISIMYPMIISEQEVDKIKVIENEVKAELTEQGIEFG
HHHCCCHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCC
NPKSGIMIETPAAVIMSRQLAKKVDFFSIGTNDLTQYTLAIDRQNTKLDDFYDAHHPAIL
CCCCCEEEECCHHHHHHHHHHHHCCEEECCCCCCEEEEEEEECCCCCCHHHHHCCCHHHH
AMIRMVVENAHAEGIWAGICGELGADTTLTEEFLKMGVDELSVSAGKVLAVRKVIRETRI
HHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]