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The map label for this gene is act [H]

Identifier: 238922271

GI number: 238922271

Start: 466544

End: 467323

Strand: Reverse

Name: act [H]

Synonym: EUBELI_20506

Alternate gene names: 238922271

Gene position: 467323-466544 (Counterclockwise)

Preceding gene: 238922272

Following gene: 238922269

Centisome position: 74.56

GC content: 39.1

Gene sequence:

>780_bases
TTGGAAGAGGAAAATGCTATGAGTGAAAGTATTAAAGGTAGAATTCATCAGGTAGAAAGCTTTGGTTCAGTTGATGGACC
AGGGGTAAGATATATTGTATTTCTTAAGGGCTGTCATATGAGATGCAGATATTGTCATAATCCTGAGACATGGAAAGAAG
AAGGCGGAACATTAGAAACAGCGCAGGAAGTGTTCGATAAAGCTTACCGTTACAGAAATTACTGGAAGAATGGTGGCGGA
ATCACTGTAAGTGGTGGAGAGGCGCTTCTTCAGATGGGATTTGTGACAGAACTCTTTGAAATTGCGAAAAAGAATGGCGT
GCATACAACTCTTGATACGTCAGGTAATCCTTTTAAGATGGAGCCGGAATATCTTGAAAAGTTTGACAGGCTGATGGCTG
TTACAGACCTTTTTCTTCTTGATATTAAGGAGATTAATGATGATAAGCACAAGGATCTTACAGGCTGGACTAATAAGAAT
ATTCTTGACCTTGCAAAGTATCTGTCTGACCACAATAAGGATATGTGGATAAGACATGTTCTTGTTCCGGGAGTGACAGA
TGCACAGGAAGATCTTGAGCAGTTAAGGGACTTTGTTGCCGGTTTAAAGACTGTTAAGAGATTTGAAGTGCTTCCATATC
ATACACTTGGAGTATTTAAGTGGGAGGAGTTAGGAATTCCATATACACTTTCTGATGTAATGCCACCTGATAAGGAACAG
GTAGCAAGAGCTAATGATATATTAAGAACAGCAGAATACACAGGATATCTTGAAAAATAA

Upstream 100 bases:

>100_bases
GTTATATTTAATGCACCAAATATATGTTTTTATAAAATGTAGACATTTTTGGAGTAAATTATCGTTATTAAGTGATAATA
CATTTATAAAGTAATAATTA

Downstream 100 bases:

>100_bases
GATATTGATAAAAGACCTCCCACAGATTCAGGGAGGTCTTTTATACGATTGTTATTTGTGATATGATCTTAATCCATCAT
TAAGCCCCTCTATATAAGAT

Product: pyruvate formate lyase activating enzyme

Products: NA

Alternate protein names: PFL-activating enzyme; Formate-C-acetyltransferase-activating enzyme [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MEEENAMSESIKGRIHQVESFGSVDGPGVRYIVFLKGCHMRCRYCHNPETWKEEGGTLETAQEVFDKAYRYRNYWKNGGG
ITVSGGEALLQMGFVTELFEIAKKNGVHTTLDTSGNPFKMEPEYLEKFDRLMAVTDLFLLDIKEINDDKHKDLTGWTNKN
ILDLAKYLSDHNKDMWIRHVLVPGVTDAQEDLEQLRDFVAGLKTVKRFEVLPYHTLGVFKWEELGIPYTLSDVMPPDKEQ
VARANDILRTAEYTGYLEK

Sequences:

>Translated_259_residues
MEEENAMSESIKGRIHQVESFGSVDGPGVRYIVFLKGCHMRCRYCHNPETWKEEGGTLETAQEVFDKAYRYRNYWKNGGG
ITVSGGEALLQMGFVTELFEIAKKNGVHTTLDTSGNPFKMEPEYLEKFDRLMAVTDLFLLDIKEINDDKHKDLTGWTNKN
ILDLAKYLSDHNKDMWIRHVLVPGVTDAQEDLEQLRDFVAGLKTVKRFEVLPYHTLGVFKWEELGIPYTLSDVMPPDKEQ
VARANDILRTAEYTGYLEK
>Mature_259_residues
MEEENAMSESIKGRIHQVESFGSVDGPGVRYIVFLKGCHMRCRYCHNPETWKEEGGTLETAQEVFDKAYRYRNYWKNGGG
ITVSGGEALLQMGFVTELFEIAKKNGVHTTLDTSGNPFKMEPEYLEKFDRLMAVTDLFLLDIKEINDDKHKDLTGWTNKN
ILDLAKYLSDHNKDMWIRHVLVPGVTDAQEDLEQLRDFVAGLKTVKRFEVLPYHTLGVFKWEELGIPYTLSDVMPPDKEQ
VARANDILRTAEYTGYLEK

Specific function: Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=240, Percent_Identity=45, Blast_Score=223, Evalue=1e-59,
Organism=Escherichia coli, GI1790389, Length=270, Percent_Identity=25.1851851851852, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1790839, Length=274, Percent_Identity=26.6423357664234, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI226510931, Length=176, Percent_Identity=30.1136363636364, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 29779; Mature: 29779

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEENAMSESIKGRIHQVESFGSVDGPGVRYIVFLKGCHMRCRYCHNPETWKEEGGTLET
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHCCCCCCHHHHCCCCHHH
AQEVFDKAYRYRNYWKNGGGITVSGGEALLQMGFVTELFEIAKKNGVHTTLDTSGNPFKM
HHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEE
EPEYLEKFDRLMAVTDLFLLDIKEINDDKHKDLTGWTNKNILDLAKYLSDHNKDMWIRHV
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEHEE
LVPGVTDAQEDLEQLRDFVAGLKTVKRFEVLPYHTLGVFKWEELGIPYTLSDVMPPDKEQ
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCEEEHHHCCCCEEHHCCCCCCHHH
VARANDILRTAEYTGYLEK
HHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MEEENAMSESIKGRIHQVESFGSVDGPGVRYIVFLKGCHMRCRYCHNPETWKEEGGTLET
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCHHCCCCCCHHHHCCCCHHH
AQEVFDKAYRYRNYWKNGGGITVSGGEALLQMGFVTELFEIAKKNGVHTTLDTSGNPFKM
HHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCEE
EPEYLEKFDRLMAVTDLFLLDIKEINDDKHKDLTGWTNKNILDLAKYLSDHNKDMWIRHV
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEHEE
LVPGVTDAQEDLEQLRDFVAGLKTVKRFEVLPYHTLGVFKWEELGIPYTLSDVMPPDKEQ
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCEEEHHHCCCCEEHHCCCCCCHHH
VARANDILRTAEYTGYLEK
HHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11029425; 10899886; 12397186 [H]