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The map label for this gene is purH [H]

Identifier: 238922187

GI number: 238922187

Start: 384195

End: 385370

Strand: Reverse

Name: purH [H]

Synonym: EUBELI_20422

Alternate gene names: 238922187

Gene position: 385370-384195 (Counterclockwise)

Preceding gene: 238922188

Following gene: 238922186

Centisome position: 61.49

GC content: 40.22

Gene sequence:

>1176_bases
ATGAACGAATTTCAGTTAAAATACGGATGCAACCCTAATCAGAAGCCTTCAAGAATATTTATGGAAGACGGAAGTGACCT
TCCTGTAGAGATTCTTAACGGCAGACCAGGATATATTAATTTTCTTGATGCATTTAACGGATACCAGCTTGTTAAGGAAT
TAAAGAAGGCTACAGGACTTCCAGCAGCTACATCTTTCAAGCATGTATCTCCAGCAGGTGCAGCAGTAGGACTTCCTCTT
ACAGATGTCGAGAAGAAGATTTACTGGGTTGATGAAGCAATCGGAGAATTAAGCCCAATCGCATGTGCGTATGCAAGAGC
AAGAGGCGCAGACAGAATGTCATCTTTCGGTGATTTCATATCATTATCAGATGTATGTGATGTTGCTACAGCAAAGCTTA
TCCAGCATGAGGTTTCAGATGGTATTGTTGCACCTGGCTATGAACCGGAGGCACTTGAGATATTAAAGTCTAAGAAGAAG
GGTAATTATAATATCATTAAGATTGACCCTGAGTATAAGCCTGAACCAATCGAGAGAAAGCAGGTGTTTGGCGTAACATT
TGAGCAGGGAAGAAATGAGTTCGTTATTGATAAGGAGCTTTTATCAAATGTTGTAACAGAGAACAAGGAAATTCCTGAAT
CAGCTAAGATTGATATGATTATTGCACTTATAACACTTAAGTATACACAGTCTAATTCTGTATGCTATGTTAAGAATGGA
CAGGCAATCGGTATCGGAGCAGGACAGCAGTCAAGAATCCATTGTACAAGACTTGCAGGACAGAAGGCAGATAACTGGTA
TCTCCGCCAGAATCCAAAGGTCCTTAATCTTCCATTTAAGGAAGGCGTAGGCCGTGCTGACAGAGATAATGCAATTGACC
TTTATATTGGTGATGAGTATGAAGATATTCTCAATGACTGGGAGAGAGTATTCACAGAGAAACCATCTGTATTTACAACA
GAGGAAAAGAAGGAATGGCTAGCAGGCAATACTGATGTAACAATCGGTTCAGATGCATTCTTCCCATTTGGAGATAATAT
AGAAAGAGCATATAAGTCAGGCGTTAAGTATGTAGCACAGCCTGGTGGTTCTGTAAGAGATGATCAGGTTATTGAAACAG
CCAATAAGCGTGGAATGGCTATGTGCTTTACAGGAATGAGACTGTTCCATCATTAA

Upstream 100 bases:

>100_bases
TAACAAGGTTTCACTTTTTGTAAGATATATTGATATTGCTACAGGTGAATACGAAACAAGAATTATTAACAAGAATGTAA
AGTAATTAAGGAGAAAGAAT

Downstream 100 bases:

>100_bases
TACAGAGCCAGTACATAAAAATGTTGCTAATTACTCATATTCATTGACTTTAGAGGTCTTATTTGTTAAAATTTATTCAA
TCAATACATGTAACAATTGA

Product: 5-aminoimidazole-4-carboxamide ribonucleotide transformylase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MNEFQLKYGCNPNQKPSRIFMEDGSDLPVEILNGRPGYINFLDAFNGYQLVKELKKATGLPAATSFKHVSPAGAAVGLPL
TDVEKKIYWVDEAIGELSPIACAYARARGADRMSSFGDFISLSDVCDVATAKLIQHEVSDGIVAPGYEPEALEILKSKKK
GNYNIIKIDPEYKPEPIERKQVFGVTFEQGRNEFVIDKELLSNVVTENKEIPESAKIDMIIALITLKYTQSNSVCYVKNG
QAIGIGAGQQSRIHCTRLAGQKADNWYLRQNPKVLNLPFKEGVGRADRDNAIDLYIGDEYEDILNDWERVFTEKPSVFTT
EEKKEWLAGNTDVTIGSDAFFPFGDNIERAYKSGVKYVAQPGGSVRDDQVIETANKRGMAMCFTGMRLFHH

Sequences:

>Translated_391_residues
MNEFQLKYGCNPNQKPSRIFMEDGSDLPVEILNGRPGYINFLDAFNGYQLVKELKKATGLPAATSFKHVSPAGAAVGLPL
TDVEKKIYWVDEAIGELSPIACAYARARGADRMSSFGDFISLSDVCDVATAKLIQHEVSDGIVAPGYEPEALEILKSKKK
GNYNIIKIDPEYKPEPIERKQVFGVTFEQGRNEFVIDKELLSNVVTENKEIPESAKIDMIIALITLKYTQSNSVCYVKNG
QAIGIGAGQQSRIHCTRLAGQKADNWYLRQNPKVLNLPFKEGVGRADRDNAIDLYIGDEYEDILNDWERVFTEKPSVFTT
EEKKEWLAGNTDVTIGSDAFFPFGDNIERAYKSGVKYVAQPGGSVRDDQVIETANKRGMAMCFTGMRLFHH
>Mature_391_residues
MNEFQLKYGCNPNQKPSRIFMEDGSDLPVEILNGRPGYINFLDAFNGYQLVKELKKATGLPAATSFKHVSPAGAAVGLPL
TDVEKKIYWVDEAIGELSPIACAYARARGADRMSSFGDFISLSDVCDVATAKLIQHEVSDGIVAPGYEPEALEILKSKKK
GNYNIIKIDPEYKPEPIERKQVFGVTFEQGRNEFVIDKELLSNVVTENKEIPESAKIDMIIALITLKYTQSNSVCYVKNG
QAIGIGAGQQSRIHCTRLAGQKADNWYLRQNPKVLNLPFKEGVGRADRDNAIDLYIGDEYEDILNDWERVFTEKPSVFTT
EEKKEWLAGNTDVTIGSDAFFPFGDNIERAYKSGVKYVAQPGGSVRDDQVIETANKRGMAMCFTGMRLFHH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=394, Percent_Identity=57.6142131979695, Blast_Score=475, Evalue=1e-134,
Organism=Escherichia coli, GI1790439, Length=397, Percent_Identity=26.9521410579345, Blast_Score=109, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71985564, Length=392, Percent_Identity=52.8061224489796, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI71985556, Length=422, Percent_Identity=47.1563981042654, Blast_Score=383, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71985574, Length=291, Percent_Identity=57.0446735395189, Blast_Score=310, Evalue=9e-85,
Organism=Saccharomyces cerevisiae, GI6323768, Length=392, Percent_Identity=56.3775510204082, Blast_Score=471, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6323056, Length=391, Percent_Identity=56.2659846547315, Blast_Score=469, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24649832, Length=393, Percent_Identity=58.2697201017812, Blast_Score=484, Evalue=1e-137,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 43559; Mature: 43559

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEFQLKYGCNPNQKPSRIFMEDGSDLPVEILNGRPGYINFLDAFNGYQLVKELKKATGL
CCCEEEEECCCCCCCCCEEEEECCCCCCEEEECCCCCEEEHHHHCCHHHHHHHHHHHCCC
PAATSFKHVSPAGAAVGLPLTDVEKKIYWVDEAIGELSPIACAYARARGADRMSSFGDFI
CCCCCCCCCCCCCCEECCCHHHHHHHHEEHHHHHCCCCHHHHHHHHHCCHHHHHHHHCHH
SLSDVCDVATAKLIQHEVSDGIVAPGYEPEALEILKSKKKGNYNIIKIDPEYKPEPIERK
HHHHHHHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCC
QVFGVTFEQGRNEFVIDKELLSNVVTENKEIPESAKIDMIIALITLKYTQSNSVCYVKNG
EEEEEEHHCCCCCEEECHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEECCCCEEEEECC
QAIGIGAGQQSRIHCTRLAGQKADNWYLRQNPKVLNLPFKEGVGRADRDNAIDLYIGDEY
CEEEECCCCCCCEEEEHHCCCCCCCCEEECCCEEEECCHHHCCCCCCCCCEEEEEECCCH
EDILNDWERVFTEKPSVFTTEEKKEWLAGNTDVTIGSDAFFPFGDNIERAYKSGVKYVAQ
HHHHHHHHHHHHCCCCEEECHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHCCCCEEEC
PGGSVRDDQVIETANKRGMAMCFTGMRLFHH
CCCCCCCCHHHHHHCCCCEEEEEHHHHHHCC
>Mature Secondary Structure
MNEFQLKYGCNPNQKPSRIFMEDGSDLPVEILNGRPGYINFLDAFNGYQLVKELKKATGL
CCCEEEEECCCCCCCCCEEEEECCCCCCEEEECCCCCEEEHHHHCCHHHHHHHHHHHCCC
PAATSFKHVSPAGAAVGLPLTDVEKKIYWVDEAIGELSPIACAYARARGADRMSSFGDFI
CCCCCCCCCCCCCCEECCCHHHHHHHHEEHHHHHCCCCHHHHHHHHHCCHHHHHHHHCHH
SLSDVCDVATAKLIQHEVSDGIVAPGYEPEALEILKSKKKGNYNIIKIDPEYKPEPIERK
HHHHHHHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCC
QVFGVTFEQGRNEFVIDKELLSNVVTENKEIPESAKIDMIIALITLKYTQSNSVCYVKNG
EEEEEEHHCCCCCEEECHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEEECCCCEEEEECC
QAIGIGAGQQSRIHCTRLAGQKADNWYLRQNPKVLNLPFKEGVGRADRDNAIDLYIGDEY
CEEEECCCCCCCEEEEHHCCCCCCCCEEECCCEEEECCHHHCCCCCCCCCEEEEEECCCH
EDILNDWERVFTEKPSVFTTEEKKEWLAGNTDVTIGSDAFFPFGDNIERAYKSGVKYVAQ
HHHHHHHHHHHHCCCCEEECHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHCCCCEEEC
PGGSVRDDQVIETANKRGMAMCFTGMRLFHH
CCCCCCCCHHHHHHCCCCEEEEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA