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The map label for this gene is cbiK [H]

Identifier: 238921957

GI number: 238921957

Start: 155659

End: 157191

Strand: Reverse

Name: cbiK [H]

Synonym: EUBELI_20192

Alternate gene names: 238921957

Gene position: 157191-155659 (Counterclockwise)

Preceding gene: 238921958

Following gene: 238921956

Centisome position: 25.08

GC content: 38.68

Gene sequence:

>1533_bases
ATGAAAAAGGCTATTCTTATAGTAAGTTTTGGAACTACATATACAGAGACAAGAATAAAGAATATTACAGCTATTAAGAA
TCAGGTATCAATGTTTTATCCTGATACGATTATAGAAGAGGCTGTATCAAGCAGGATTGTAAGAAATCATATGAAGCAGG
ATGAGCATATTGATGCAAAAAGTCCGCTGGAAGCCCTTATGAGTCTAAAAGAGCAGGGCGTAACCGGGGTTGTGGTATTT
CCAACTCATGTAATAGATGGCATAGAGAATAATCAGATGAAAAATGCAGTTGAACAATGCAGAACTTTTTTTGAAAAGAT
AGTTACAGCTGATGCACTTTTGTCAAATGTGGATGATTACACAGATGTTTCGAAGGCACTGTGGGAGTCATTAAAGGAAA
TAGCCGGTGATTCACCTTTGATTTTTATGGGGCATGGAACGGAGCATTCGGCTGATGAAAGCTATATGATAATTGAGCAG
GCATTAAGGGCGTATGCCAGACATCCTGTTTATATTGCAACAGTTGAGGGAAAGAGAACAATTCAGGATGTAATCAGGCA
GATGAAAGATGAAGGGATTGTTAATACAAGAGTAGTTCTTGCACCATTTATGTTAGTGGCAGGAGATCATGCCAATAATG
ATATGGCAGGAGAGAGTGATTCATTTGCAAGTATATTAAAAGAAGAAGGATATGCGCCGGAATGTGTCCTGAAAGGAATT
GGCGAATATCCGGCAGTAAGACAGGTGTATCTGTCACATTTGCAGAAAGCCGTAACACAGCTTGAGTACATACCGGATGA
ACAATCAGGCATATTGTATGGTATTGGAGTTGGCACAGGTAATCCGAAGCAGATGACATTGCAGGAACTGGAGGTAATCA
GGGAGTGCGACCTGATAGTGATTCCGGCAGTGTCAAAGGAAGAGTGTTATGCATACCGCATAGCAGAGCAGGCTTATCAT
GAAATTTCGGAAAAGCCTGTATTATGCATGCCATTTCCAATGATTAAGGATGAAGGAAAACTTGAAATTTCACATAATAA
AATATATGAAAACATAGAAGGATATTTGAGCAAAGGCCAGAAAGTAGGAATGCTTACAATAGGTGATCCGTCTATATATT
CTACATATATGTACATGCATAAAAGAGCAGAAGCAAATGGCTGGAGAGCACAGATAATATCTGGTGTTCCTTCATTTTGC
TCTGTTGCAGCAAGACTTGGGATATCACTTGGAGAGAAGAATGAAGAAATTCATATTATCCCGTCAGCGTATAAACCTGA
GGATACATTTTCATATGATGGAACATGTGTGTATATGAAGTCGGGAAAGGGGCTGAAAGCGTTGATTTCAGCACTGCGTC
TCAGACAGGAAACAACAGGGGAATCATATGAAATAAATGCAGTTTCGAATTGTGGCATGGACAATGAAAAGATATATCAT
GGTCTGGATGAACTTGAAGAGGCACAAGGGTATCTTACAACAGTTATAGTAAAAAAGAAAAACAATTGCATTTCTAGTAA
TAATATGGAATAA

Upstream 100 bases:

>100_bases
AAAGGATTGTACGAATTGTACATTTCCGCATATTCCGGAAAATTATGATTACATAGTAAAGTTTTTATCTGAAAAGATGA
GATAGGAGAAGAAACTCACA

Downstream 100 bases:

>100_bases
TAAACGCTGGATATAATCCAGATGATAACGAAATAATCACTGTAAGCGAAGTATATATGTACTTCAGGGAATCAGGTGGG
AATCCTGAACAGTTCCGCTG

Product: sirohydrochlorin cobaltochelatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MKKAILIVSFGTTYTETRIKNITAIKNQVSMFYPDTIIEEAVSSRIVRNHMKQDEHIDAKSPLEALMSLKEQGVTGVVVF
PTHVIDGIENNQMKNAVEQCRTFFEKIVTADALLSNVDDYTDVSKALWESLKEIAGDSPLIFMGHGTEHSADESYMIIEQ
ALRAYARHPVYIATVEGKRTIQDVIRQMKDEGIVNTRVVLAPFMLVAGDHANNDMAGESDSFASILKEEGYAPECVLKGI
GEYPAVRQVYLSHLQKAVTQLEYIPDEQSGILYGIGVGTGNPKQMTLQELEVIRECDLIVIPAVSKEECYAYRIAEQAYH
EISEKPVLCMPFPMIKDEGKLEISHNKIYENIEGYLSKGQKVGMLTIGDPSIYSTYMYMHKRAEANGWRAQIISGVPSFC
SVAARLGISLGEKNEEIHIIPSAYKPEDTFSYDGTCVYMKSGKGLKALISALRLRQETTGESYEINAVSNCGMDNEKIYH
GLDELEEAQGYLTTVIVKKKNNCISSNNME

Sequences:

>Translated_510_residues
MKKAILIVSFGTTYTETRIKNITAIKNQVSMFYPDTIIEEAVSSRIVRNHMKQDEHIDAKSPLEALMSLKEQGVTGVVVF
PTHVIDGIENNQMKNAVEQCRTFFEKIVTADALLSNVDDYTDVSKALWESLKEIAGDSPLIFMGHGTEHSADESYMIIEQ
ALRAYARHPVYIATVEGKRTIQDVIRQMKDEGIVNTRVVLAPFMLVAGDHANNDMAGESDSFASILKEEGYAPECVLKGI
GEYPAVRQVYLSHLQKAVTQLEYIPDEQSGILYGIGVGTGNPKQMTLQELEVIRECDLIVIPAVSKEECYAYRIAEQAYH
EISEKPVLCMPFPMIKDEGKLEISHNKIYENIEGYLSKGQKVGMLTIGDPSIYSTYMYMHKRAEANGWRAQIISGVPSFC
SVAARLGISLGEKNEEIHIIPSAYKPEDTFSYDGTCVYMKSGKGLKALISALRLRQETTGESYEINAVSNCGMDNEKIYH
GLDELEEAQGYLTTVIVKKKNNCISSNNME
>Mature_510_residues
MKKAILIVSFGTTYTETRIKNITAIKNQVSMFYPDTIIEEAVSSRIVRNHMKQDEHIDAKSPLEALMSLKEQGVTGVVVF
PTHVIDGIENNQMKNAVEQCRTFFEKIVTADALLSNVDDYTDVSKALWESLKEIAGDSPLIFMGHGTEHSADESYMIIEQ
ALRAYARHPVYIATVEGKRTIQDVIRQMKDEGIVNTRVVLAPFMLVAGDHANNDMAGESDSFASILKEEGYAPECVLKGI
GEYPAVRQVYLSHLQKAVTQLEYIPDEQSGILYGIGVGTGNPKQMTLQELEVIRECDLIVIPAVSKEECYAYRIAEQAYH
EISEKPVLCMPFPMIKDEGKLEISHNKIYENIEGYLSKGQKVGMLTIGDPSIYSTYMYMHKRAEANGWRAQIISGVPSFC
SVAARLGISLGEKNEEIHIIPSAYKPEDTFSYDGTCVYMKSGKGLKALISALRLRQETTGESYEINAVSNCGMDNEKIYH
GLDELEEAQGYLTTVIVKKKNNCISSNNME

Specific function: Chelates cobalt to the cobalamin precursor as part of the anaerobic pathway to cobalamin biosynthesis [H]

COG id: COG4822

COG function: function code H; Cobalamin biosynthesis protein CbiK, Co2+ chelatase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CbiK family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010388 [H]

Pfam domain/function: PF06180 CbiK [H]

EC number: =4.99.1.3 [H]

Molecular weight: Translated: 56882; Mature: 56882

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKAILIVSFGTTYTETRIKNITAIKNQVSMFYPDTIIEEAVSSRIVRNHMKQDEHIDAK
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
SPLEALMSLKEQGVTGVVVFPTHVIDGIENNQMKNAVEQCRTFFEKIVTADALLSNVDDY
CHHHHHHHHHHCCCCEEEEECHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
TDVSKALWESLKEIAGDSPLIFMGHGTEHSADESYMIIEQALRAYARHPVYIATVEGKRT
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCHHH
IQDVIRQMKDEGIVNTRVVLAPFMLVAGDHANNDMAGESDSFASILKEEGYAPECVLKGI
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHCC
GEYPAVRQVYLSHLQKAVTQLEYIPDEQSGILYGIGVGTGNPKQMTLQELEVIRECDLIV
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEE
IPAVSKEECYAYRIAEQAYHEISEKPVLCMPFPMIKDEGKLEISHNKIYENIEGYLSKGQ
EECCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEHHHHHHHHHHHHCCCC
KVGMLTIGDPSIYSTYMYMHKRAEANGWRAQIISGVPSFCSVAARLGISLGEKNEEIHII
EEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEE
PSAYKPEDTFSYDGTCVYMKSGKGLKALISALRLRQETTGESYEINAVSNCGMDNEKIYH
CCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEHHCCCCCHHHHHH
GLDELEEAQGYLTTVIVKKKNNCISSNNME
CHHHHHHHCCEEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MKKAILIVSFGTTYTETRIKNITAIKNQVSMFYPDTIIEEAVSSRIVRNHMKQDEHIDAK
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
SPLEALMSLKEQGVTGVVVFPTHVIDGIENNQMKNAVEQCRTFFEKIVTADALLSNVDDY
CHHHHHHHHHHCCCCEEEEECHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
TDVSKALWESLKEIAGDSPLIFMGHGTEHSADESYMIIEQALRAYARHPVYIATVEGKRT
HHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCHHH
IQDVIRQMKDEGIVNTRVVLAPFMLVAGDHANNDMAGESDSFASILKEEGYAPECVLKGI
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHCC
GEYPAVRQVYLSHLQKAVTQLEYIPDEQSGILYGIGVGTGNPKQMTLQELEVIRECDLIV
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEE
IPAVSKEECYAYRIAEQAYHEISEKPVLCMPFPMIKDEGKLEISHNKIYENIEGYLSKGQ
EECCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEHHHHHHHHHHHHCCCC
KVGMLTIGDPSIYSTYMYMHKRAEANGWRAQIISGVPSFCSVAARLGISLGEKNEEIHII
EEEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEE
PSAYKPEDTFSYDGTCVYMKSGKGLKALISALRLRQETTGESYEINAVSNCGMDNEKIYH
CCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEHHCCCCCHHHHHH
GLDELEEAQGYLTTVIVKKKNNCISSNNME
CHHHHHHHCCEEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609; 9150215; 10451360 [H]