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The map label for this gene is cbiF [H]

Identifier: 238921951

GI number: 238921951

Start: 149346

End: 150092

Strand: Reverse

Name: cbiF [H]

Synonym: EUBELI_20185

Alternate gene names: 238921951

Gene position: 150092-149346 (Counterclockwise)

Preceding gene: 238921952

Following gene: 238921950

Centisome position: 23.95

GC content: 40.43

Gene sequence:

>747_bases
ATGGTTAAAATTGTCGGGGCAGGACCCGGAGCTAAAGATTTAATTACAGTTCGCGGCATGCGCATGCTTAATGAGGCAGA
TGTGATTATATATGCAGGTTCACTTGTAAATGAAGAACTGCTTGAATATGCCAAAAAAGACTGTGAGATATATAACAGTG
CATACATGAATCTCGATGAGGTCATTAATGTAATTGTGAAGGCGGAGAGCGAAGGAAAGAATATAGTACGACTGCATACA
GGAGATCCTTCACTTTATGGAGCTATAAGGGAGCAGATTGAGGAACTTGCAAAGCATGGAATTACATGTGAAATATGTCC
GGGTGTCAGCAGCTTTCTTGCTGCGGCAGCTGCAATGAACTGTGAATATACAGTGCCGGAGGTTGCACAGTCAGTAATTA
TTACAAGGATGCCGGGAAGGACGCCTGTTCCGGATAATGAGAGCATTGCAAAGCTGGCGGCACATGGAACCTCAATGGTT
ATATTCTTAAGTTCATCACATTTAAAGGAGCTTAAGGAAGAATTATATAAGGGAGCGTATACACCGGATACTCCATGTGC
AATATGTTATAAGGCAAGCTGGAAGGATGAAAAATGTATAAAGACCACATTGGAAAATCTTGACAGAGAAGCCGAAAAGG
CAGGAATTACAAAGACAGCACTTATAATGGTGGGAGAATTCTTAGGAGATAAATATTCATTTTCCAAGTTATATGATCCT
GAATTTAAAACGGAGTATAGAAAATGA

Upstream 100 bases:

>100_bases
AAAATAAGAATCGGGGCAATTGTATTCTCTAATGAATATGGAATATTAGGTAAGACAAAAACAGCGGATATGCTGTTAGA
AGATATAATGGAGAATAATT

Downstream 100 bases:

>100_bases
GAACTGTTATAATAGCATTTACACATGAGGGAATGAAGGTGGCAAAAAAGGCTTCCACGGTGACGGATGAAGAAGTTACA
ATTTACTGCCACAAAAGATG

Product: precorrin-4 C11-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MVKIVGAGPGAKDLITVRGMRMLNEADVIIYAGSLVNEELLEYAKKDCEIYNSAYMNLDEVINVIVKAESEGKNIVRLHT
GDPSLYGAIREQIEELAKHGITCEICPGVSSFLAAAAAMNCEYTVPEVAQSVIITRMPGRTPVPDNESIAKLAAHGTSMV
IFLSSSHLKELKEELYKGAYTPDTPCAICYKASWKDEKCIKTTLENLDREAEKAGITKTALIMVGEFLGDKYSFSKLYDP
EFKTEYRK

Sequences:

>Translated_248_residues
MVKIVGAGPGAKDLITVRGMRMLNEADVIIYAGSLVNEELLEYAKKDCEIYNSAYMNLDEVINVIVKAESEGKNIVRLHT
GDPSLYGAIREQIEELAKHGITCEICPGVSSFLAAAAAMNCEYTVPEVAQSVIITRMPGRTPVPDNESIAKLAAHGTSMV
IFLSSSHLKELKEELYKGAYTPDTPCAICYKASWKDEKCIKTTLENLDREAEKAGITKTALIMVGEFLGDKYSFSKLYDP
EFKTEYRK
>Mature_248_residues
MVKIVGAGPGAKDLITVRGMRMLNEADVIIYAGSLVNEELLEYAKKDCEIYNSAYMNLDEVINVIVKAESEGKNIVRLHT
GDPSLYGAIREQIEELAKHGITCEICPGVSSFLAAAAAMNCEYTVPEVAQSVIITRMPGRTPVPDNESIAKLAAHGTSMV
IFLSSSHLKELKEELYKGAYTPDTPCAICYKASWKDEKCIKTTLENLDREAEKAGITKTALIMVGEFLGDKYSFSKLYDP
EFKTEYRK

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=241, Percent_Identity=25.3112033195021, Blast_Score=93, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6322922, Length=235, Percent_Identity=25.531914893617, Blast_Score=77, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 27361; Mature: 27361

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00839 SUMT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKIVGAGPGAKDLITVRGMRMLNEADVIIYAGSLVNEELLEYAKKDCEIYNSAYMNLDE
CEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCHHH
VINVIVKAESEGKNIVRLHTGDPSLYGAIREQIEELAKHGITCEICPGVSSFLAAAAAMN
HHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHCC
CEYTVPEVAQSVIITRMPGRTPVPDNESIAKLAAHGTSMVIFLSSSHLKELKEELYKGAY
CCCCHHHHHHHHHHEECCCCCCCCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCC
TPDTPCAICYKASWKDEKCIKTTLENLDREAEKAGITKTALIMVGEFLGDKYSFSKLYDP
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHCCCC
EFKTEYRK
CHHHCCCC
>Mature Secondary Structure
MVKIVGAGPGAKDLITVRGMRMLNEADVIIYAGSLVNEELLEYAKKDCEIYNSAYMNLDE
CEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCHHH
VINVIVKAESEGKNIVRLHTGDPSLYGAIREQIEELAKHGITCEICPGVSSFLAAAAAMN
HHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHCC
CEYTVPEVAQSVIITRMPGRTPVPDNESIAKLAAHGTSMVIFLSSSHLKELKEELYKGAY
CCCCHHHHHHHHHHEECCCCCCCCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHHHCCCC
TPDTPCAICYKASWKDEKCIKTTLENLDREAEKAGITKTALIMVGEFLGDKYSFSKLYDP
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHCCCC
EFKTEYRK
CHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]