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The map label for this gene is lpdA [H]

Identifier: 238921682

GI number: 238921682

Start: 3723093

End: 3724541

Strand: Direct

Name: lpdA [H]

Synonym: NT01EI_3843

Alternate gene names: 238921682

Gene position: 3723093-3724541 (Clockwise)

Preceding gene: 238921681

Following gene: 238921688

Centisome position: 97.66

GC content: 61.7

Gene sequence:

>1449_bases
ATGAGAGAATTACACGTCGACGTTGCCGTGATCGGCGGCGGCACCGCTGGATTAGGCGCCTATCGTGCGGCCAAACGCCA
TACTCCCAGCGTGGTGATGATTGAAGGCGGTCCCTTTGGCACCACCTGCGCCCGCGTGGGCTGCATGCCCTCTAAGCTGC
TGATCGCCGCGGCAGAGTCCGTGCATCAGATCGCCCGAGCACCCGGCTTCGGTATCCACCCCCAGGGCGACGTCCGCATT
GACGGTCGAGAGGTGATGGATCGCGTGAAGCGCGAACGCGATCGCTTCGTCGGGTTCGTCATGGAGGGTGTCGACAGCAT
TCCCACGGATGATAAAATCCTTGGTTATGCCCGTTTTCTGGACGATAACACTCTGCAGGTTGATGAGCATACCCGTATCC
ATGCCAAACGCATCGTCATCGCCAGCGGCTCACGTCCGGTATGGCCTTCCGCCTGGGATGCCCTGGGCGATCGTCTGATC
GTCAACGACGATCTGTTCGCCTGGGATACGCTGCCACGCACCGTTGCCGTCTTCGGCCCCGGCGTCATCGGTCTTGAGCT
GGGACAGGCCCTGCACCGGCTCGGCGTCGAGGTCAGGGTATTCGGCGTCGGCGGCGCCGTCGGGCCGCTGACGGATACCG
AGGTCCGCCGCTATGCCGTAGATAAACTGGGCGCTGAGTTTTATCTCGACCCCGATGCACAGGTAGAGATCCTACAGCGT
GAAGGGGCGCAAGTCTTCATTCGCTATCGCGATCTGAACGGTCAGACGCAGGATATCCTGGTGGACTACGTTCTGGCCGC
CACCGGTCGGCGCCCCAACCTCGACCGCCTGGGGCTGGAGAACACCACCCTGCGTCTGGATGCGCGCGGCGTACCGCTGG
CAGATCGCCTGACAATGCAGACCAGCACCGAACATATCTTTATTGCCGGTGACGCCAGCAACCAGCTGCCGCTGCTCCAT
GAGGCCAGCGATCAGGCGCGCATTGCCGGTGAGAATGCCGGCAGCTATCCCCTGATAGCCCCTGGACTGCGCCGCAGCCC
CCTCGCGGTGGTCTTTTCCGATCCGCAGATCGCGATGGTGGGCTCCACCTTCCGCGAGCTGAATGAGAAATATGGCGCCT
GCAGCTGCTTCGCCGTCGGGGAGGTCTCATTTGAAAACCAGGGACGATCGCGCGTCATGCTGCGTAATCTGGGCCTGCTG
CATGTCTATGCTGAGCAGGGAAGCGGTCGCTTCCTCGGTGCGGAGATGATCGGACCGGACGCCGAACACCTTGCCCATTT
GCTCTCCTGGGCCCATCAACAGCAAATGACCGTAGATCAAATGCTGGATATGCCGTTTTATCATCCGGTCATTGAGGAGG
GGGTACGTACCGCCCTGCGCGATCTGCAAAGCAAACTGCGTCTGGGCAGCGACGAAATCGAGCGCTGCCTGCGCTGCCCG
GGGGATTAA

Upstream 100 bases:

>100_bases
TGCAACAGGCTTAACAACAATAGGGCCACCCATGGCCCATCAAAGCCAACGTATTGGCGGGCTTGTCCCGCCTTTTTTTC
GGTATTCCAGGAATCATGTA

Downstream 100 bases:

>100_bases
CCCCGCCGGCGCGCTGCGCATCAGAGAATGTTACCCAGTCGCCGCTGGGCATCATCGATCGCCTGTGCGACCTGCTGCGG
CGCCACGCCGCCGCGGGCGC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MRELHVDVAVIGGGTAGLGAYRAAKRHTPSVVMIEGGPFGTTCARVGCMPSKLLIAAAESVHQIARAPGFGIHPQGDVRI
DGREVMDRVKRERDRFVGFVMEGVDSIPTDDKILGYARFLDDNTLQVDEHTRIHAKRIVIASGSRPVWPSAWDALGDRLI
VNDDLFAWDTLPRTVAVFGPGVIGLELGQALHRLGVEVRVFGVGGAVGPLTDTEVRRYAVDKLGAEFYLDPDAQVEILQR
EGAQVFIRYRDLNGQTQDILVDYVLAATGRRPNLDRLGLENTTLRLDARGVPLADRLTMQTSTEHIFIAGDASNQLPLLH
EASDQARIAGENAGSYPLIAPGLRRSPLAVVFSDPQIAMVGSTFRELNEKYGACSCFAVGEVSFENQGRSRVMLRNLGLL
HVYAEQGSGRFLGAEMIGPDAEHLAHLLSWAHQQQMTVDQMLDMPFYHPVIEEGVRTALRDLQSKLRLGSDEIERCLRCP
GD

Sequences:

>Translated_482_residues
MRELHVDVAVIGGGTAGLGAYRAAKRHTPSVVMIEGGPFGTTCARVGCMPSKLLIAAAESVHQIARAPGFGIHPQGDVRI
DGREVMDRVKRERDRFVGFVMEGVDSIPTDDKILGYARFLDDNTLQVDEHTRIHAKRIVIASGSRPVWPSAWDALGDRLI
VNDDLFAWDTLPRTVAVFGPGVIGLELGQALHRLGVEVRVFGVGGAVGPLTDTEVRRYAVDKLGAEFYLDPDAQVEILQR
EGAQVFIRYRDLNGQTQDILVDYVLAATGRRPNLDRLGLENTTLRLDARGVPLADRLTMQTSTEHIFIAGDASNQLPLLH
EASDQARIAGENAGSYPLIAPGLRRSPLAVVFSDPQIAMVGSTFRELNEKYGACSCFAVGEVSFENQGRSRVMLRNLGLL
HVYAEQGSGRFLGAEMIGPDAEHLAHLLSWAHQQQMTVDQMLDMPFYHPVIEEGVRTALRDLQSKLRLGSDEIERCLRCP
GD
>Mature_482_residues
MRELHVDVAVIGGGTAGLGAYRAAKRHTPSVVMIEGGPFGTTCARVGCMPSKLLIAAAESVHQIARAPGFGIHPQGDVRI
DGREVMDRVKRERDRFVGFVMEGVDSIPTDDKILGYARFLDDNTLQVDEHTRIHAKRIVIASGSRPVWPSAWDALGDRLI
VNDDLFAWDTLPRTVAVFGPGVIGLELGQALHRLGVEVRVFGVGGAVGPLTDTEVRRYAVDKLGAEFYLDPDAQVEILQR
EGAQVFIRYRDLNGQTQDILVDYVLAATGRRPNLDRLGLENTTLRLDARGVPLADRLTMQTSTEHIFIAGDASNQLPLLH
EASDQARIAGENAGSYPLIAPGLRRSPLAVVFSDPQIAMVGSTFRELNEKYGACSCFAVGEVSFENQGRSRVMLRNLGLL
HVYAEQGSGRFLGAEMIGPDAEHLAHLLSWAHQQQMTVDQMLDMPFYHPVIEEGVRTALRDLQSKLRLGSDEIERCLRCP
GD

Specific function: Lipoamide Dehydrogenase Is A Component Of The Glycine Cleavage System As Well As Of The Alpha-Ketoacid Dehydrogenase Complexes. [C]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=469, Percent_Identity=26.6524520255864, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI50301238, Length=386, Percent_Identity=25.6476683937824, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1786307, Length=475, Percent_Identity=24.2105263157895, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI87082354, Length=476, Percent_Identity=25.8403361344538, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1789915, Length=365, Percent_Identity=29.3150684931507, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87081717, Length=479, Percent_Identity=22.5469728601253, Blast_Score=86, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI32565766, Length=469, Percent_Identity=28.1449893390192, Blast_Score=148, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI71983429, Length=371, Percent_Identity=27.7628032345013, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71983419, Length=371, Percent_Identity=27.7628032345013, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321091, Length=480, Percent_Identity=25.4166666666667, Blast_Score=124, Evalue=4e-29,
Organism=Saccharomyces cerevisiae, GI6325166, Length=397, Percent_Identity=25.9445843828715, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6325240, Length=363, Percent_Identity=25.068870523416, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21358499, Length=472, Percent_Identity=27.7542372881356, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24640553, Length=494, Percent_Identity=25.3036437246964, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24640549, Length=496, Percent_Identity=25.4032258064516, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24640551, Length=496, Percent_Identity=25.4032258064516, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17737741, Length=493, Percent_Identity=26.1663286004057, Blast_Score=89, Evalue=8e-18,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 52816; Mature: 52816

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRELHVDVAVIGGGTAGLGAYRAAKRHTPSVVMIEGGPFGTTCARVGCMPSKLLIAAAES
CCEEEEEEEEECCCCCCHHHHHHHHCCCCCEEEECCCCCCHHHHHHCCCCHHHHHHHHHH
VHQIARAPGFGIHPQGDVRIDGREVMDRVKRERDRFVGFVMEGVDSIPTDDKILGYARFL
HHHHHHCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEHEEC
DDNTLQVDEHTRIHAKRIVIASGSRPVWPSAWDALGDRLIVNDDLFAWDTLPRTVAVFGP
CCCCEEECCCCEEEEEEEEEECCCCCCCCCHHHHCCCEEEECCCCEEECCCCCEEEEECC
GVIGLELGQALHRLGVEVRVFGVGGAVGPLTDTEVRRYAVDKLGAEFYLDPDAQVEILQR
CCHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEECCCCHHHHEEC
EGAQVFIRYRDLNGQTQDILVDYVLAATGRRPNLDRLGLENTTLRLDARGVPLADRLTMQ
CCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHCCCCCCEEEEECCCCCHHHHEEEE
TSTEHIFIAGDASNQLPLLHEASDQARIAGENAGSYPLIAPGLRRSPLAVVFSDPQIAMV
ECCCEEEEEECCCCCCCEEECCCCCCEEECCCCCCCCEECCCCCCCCEEEEECCCCEEEH
GSTFRELNEKYGACSCFAVGEVSFENQGRSRVMLRNLGLLHVYAEQGSGRFLGAEMIGPD
HHHHHHHHHHCCCEEEEEEECEEECCCCCCEEHEECCCEEEEEEECCCCEEEEHHHCCCC
AEHLAHLLSWAHQQQMTVDQMLDMPFYHPVIEEGVRTALRDLQSKLRLGSDEIERCLRCP
HHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
GD
CC
>Mature Secondary Structure
MRELHVDVAVIGGGTAGLGAYRAAKRHTPSVVMIEGGPFGTTCARVGCMPSKLLIAAAES
CCEEEEEEEEECCCCCCHHHHHHHHCCCCCEEEECCCCCCHHHHHHCCCCHHHHHHHHHH
VHQIARAPGFGIHPQGDVRIDGREVMDRVKRERDRFVGFVMEGVDSIPTDDKILGYARFL
HHHHHHCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEHEEC
DDNTLQVDEHTRIHAKRIVIASGSRPVWPSAWDALGDRLIVNDDLFAWDTLPRTVAVFGP
CCCCEEECCCCEEEEEEEEEECCCCCCCCCHHHHCCCEEEECCCCEEECCCCCEEEEECC
GVIGLELGQALHRLGVEVRVFGVGGAVGPLTDTEVRRYAVDKLGAEFYLDPDAQVEILQR
CCHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEECCCCHHHHEEC
EGAQVFIRYRDLNGQTQDILVDYVLAATGRRPNLDRLGLENTTLRLDARGVPLADRLTMQ
CCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHCCCCCCEEEEECCCCCHHHHEEEE
TSTEHIFIAGDASNQLPLLHEASDQARIAGENAGSYPLIAPGLRRSPLAVVFSDPQIAMV
ECCCEEEEEECCCCCCCEEECCCCCCEEECCCCCCCCEECCCCCCCCEEEEECCCCEEEH
GSTFRELNEKYGACSCFAVGEVSFENQGRSRVMLRNLGLLHVYAEQGSGRFLGAEMIGPD
HHHHHHHHHHCCCEEEEEEECEEECCCCCCEEHEECCCEEEEEEECCCCEEEEHHHCCCC
AEHLAHLLSWAHQQQMTVDQMLDMPFYHPVIEEGVRTALRDLQSKLRLGSDEIERCLRCP
HHHHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCC
GD
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1339281; 1581034 [H]