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The map label for this gene is glpF [H]

Identifier: 238921638

GI number: 238921638

Start: 3674311

End: 3675153

Strand: Reverse

Name: glpF [H]

Synonym: NT01EI_3793

Alternate gene names: 238921638

Gene position: 3675153-3674311 (Counterclockwise)

Preceding gene: 238921641

Following gene: 238921637

Centisome position: 96.4

GC content: 59.79

Gene sequence:

>843_bases
ATGAGCCAGATCGCGAGTACGACATTAAAAGGACAGTGTATCGCCGAATTCCTGGGCACCGCCCTGCTGATATTCTTCGG
CTGTGGTTGCGTGGCCGCCCTGAAACTGGCCGGAGCTACTTTGAGTCTGTGGGAGATCAGCGTCATTTGGGGCTTGGGGG
TCGCTCTGGCTATCTACCTGACGGCCGCTATCTCCGGCGCCCACCTCAACCCGGCGGTCACCATCGCTCTGTGGCTGTTT
GCCTGCTTTGAGCGCCGCAAAGTGCTCCCCTACATCATCGCCCAAGTCGCCGGCGCATTCTGTGCCGCTGCGCTGGTCTA
TGCCATGTACCACAATCTGTTCATCGACTATGAGCAAACCCATCATATGGTACGCGGCAGCGAAGAGAGCCTGACGCTGG
CGGGGATCTTCTCCACCTACCCGAACCCGCATATTACCGTATGGCAGGCGCTGCTGGTTGAAACCGTCATCACCGCCATT
CTGATGTGTCTGATTCTGGCGCTGACCGACGATGGTAATGGCATTCCGCGTGGGCCGCTGGCACCGCTGCTGATTGGTGT
CCTGATCGCCGTCATTGGCGCCGCCATGGGGCCCTTGACCGGCTTTGCCCTTAACCCGGCCCGTGACTTCGGCCCGAAAG
CCTTTGCCTGGATGGCCGGCTGGGGTGAGATTGCCTTCACCGGCGGGCGCAGCATTCCCTACTTCCTGGTTCCCATCGTT
GGCCCAATTATCGGTGCCTGCCTGGGGGCCTTTGGCTACCGCGCGCTGATCGGTCGTCACCTCCCCTGCGATGTCTGCAT
CGCAGAAGAAGAGAAAAAGAACAGCGAGAAATCCAAAGCCTGA

Upstream 100 bases:

>100_bases
CTCGTTTTCGCTCGTTAACGATAAATTCACATCGCACCCGTAACCTGCTCCATTCAGAGCCGGCATTTATCTTCCACCTC
TTCGTCGCAGGATTTCTTTT

Downstream 100 bases:

>100_bases
TAAGGTTTCCGGGGAGGGTTCTCCCCGGCATTTGAGCGAACTCCGTACACCGACCCCCAACCAGGACAACGATCATGACA
ACCGAACAAAAATACATTGT

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: Aquaglyceroporin [H]

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MSQIASTTLKGQCIAEFLGTALLIFFGCGCVAALKLAGATLSLWEISVIWGLGVALAIYLTAAISGAHLNPAVTIALWLF
ACFERRKVLPYIIAQVAGAFCAAALVYAMYHNLFIDYEQTHHMVRGSEESLTLAGIFSTYPNPHITVWQALLVETVITAI
LMCLILALTDDGNGIPRGPLAPLLIGVLIAVIGAAMGPLTGFALNPARDFGPKAFAWMAGWGEIAFTGGRSIPYFLVPIV
GPIIGACLGAFGYRALIGRHLPCDVCIAEEEKKNSEKSKA

Sequences:

>Translated_280_residues
MSQIASTTLKGQCIAEFLGTALLIFFGCGCVAALKLAGATLSLWEISVIWGLGVALAIYLTAAISGAHLNPAVTIALWLF
ACFERRKVLPYIIAQVAGAFCAAALVYAMYHNLFIDYEQTHHMVRGSEESLTLAGIFSTYPNPHITVWQALLVETVITAI
LMCLILALTDDGNGIPRGPLAPLLIGVLIAVIGAAMGPLTGFALNPARDFGPKAFAWMAGWGEIAFTGGRSIPYFLVPIV
GPIIGACLGAFGYRALIGRHLPCDVCIAEEEKKNSEKSKA
>Mature_279_residues
SQIASTTLKGQCIAEFLGTALLIFFGCGCVAALKLAGATLSLWEISVIWGLGVALAIYLTAAISGAHLNPAVTIALWLFA
CFERRKVLPYIIAQVAGAFCAAALVYAMYHNLFIDYEQTHHMVRGSEESLTLAGIFSTYPNPHITVWQALLVETVITAIL
MCLILALTDDGNGIPRGPLAPLLIGVLIAVIGAAMGPLTGFALNPARDFGPKAFAWMAGWGEIAFTGGRSIPYFLVPIVG
PIIGACLGAFGYRALIGRHLPCDVCIAEEEKKNSEKSKA

Specific function: Transporter of glycerol across the cytoplasmic membrane, with limited permeability to water and small uncharged compounds such as polyols [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=278, Percent_Identity=36.3309352517986, Blast_Score=171, Evalue=8e-43,
Organism=Homo sapiens, GI4826645, Length=263, Percent_Identity=39.1634980988593, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI22538420, Length=274, Percent_Identity=36.1313868613139, Blast_Score=161, Evalue=5e-40,
Organism=Homo sapiens, GI4502187, Length=271, Percent_Identity=33.210332103321, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI310133356, Length=181, Percent_Identity=32.5966850828729, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI310114181, Length=181, Percent_Identity=32.5966850828729, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI45446752, Length=205, Percent_Identity=31.7073170731707, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI4502183, Length=293, Percent_Identity=27.9863481228669, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI6912506, Length=258, Percent_Identity=30.6201550387597, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI4502179, Length=256, Percent_Identity=31.640625, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI86792455, Length=203, Percent_Identity=33.0049261083744, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI37694062, Length=204, Percent_Identity=30.8823529411765, Blast_Score=68, Evalue=8e-12,
Organism=Escherichia coli, GI1790362, Length=279, Percent_Identity=76.7025089605735, Blast_Score=453, Evalue=1e-129,
Organism=Escherichia coli, GI1787101, Length=200, Percent_Identity=32.5, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71992966, Length=260, Percent_Identity=36.1538461538462, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI71994009, Length=261, Percent_Identity=33.3333333333333, Blast_Score=150, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI32564052, Length=281, Percent_Identity=32.0284697508897, Blast_Score=146, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17533613, Length=281, Percent_Identity=32.0284697508897, Blast_Score=146, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17531429, Length=260, Percent_Identity=35.3846153846154, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17531431, Length=260, Percent_Identity=35.3846153846154, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17544068, Length=265, Percent_Identity=35.0943396226415, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI71992961, Length=260, Percent_Identity=32.6923076923077, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71993722, Length=252, Percent_Identity=28.5714285714286, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17558372, Length=257, Percent_Identity=29.5719844357977, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6321054, Length=216, Percent_Identity=38.8888888888889, Blast_Score=142, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6322985, Length=307, Percent_Identity=27.6872964169381, Blast_Score=99, Evalue=8e-22,
Organism=Drosophila melanogaster, GI45550503, Length=248, Percent_Identity=28.2258064516129, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24762344, Length=268, Percent_Identity=29.4776119402985, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24762346, Length=268, Percent_Identity=29.4776119402985, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24762348, Length=268, Percent_Identity=29.4776119402985, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI20130305, Length=268, Percent_Identity=29.4776119402985, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24652747, Length=241, Percent_Identity=30.2904564315353, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45551084, Length=241, Percent_Identity=30.2904564315353, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24762342, Length=243, Percent_Identity=26.3374485596708, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI19922828, Length=201, Percent_Identity=27.363184079602, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 29712; Mature: 29581

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQIASTTLKGQCIAEFLGTALLIFFGCGCVAALKLAGATLSLWEISVIWGLGVALAIYL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
TAAISGAHLNPAVTIALWLFACFERRKVLPYIIAQVAGAFCAAALVYAMYHNLFIDYEQT
HHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
HHMVRGSEESLTLAGIFSTYPNPHITVWQALLVETVITAILMCLILALTDDGNGIPRGPL
HHHHCCCCCCEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
APLLIGVLIAVIGAAMGPLTGFALNPARDFGPKAFAWMAGWGEIAFTGGRSIPYFLVPIV
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHCCCCCEEEECCCCCCHHHHHHH
GPIIGACLGAFGYRALIGRHLPCDVCIAEEEKKNSEKSKA
HHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHCCCCCCCC
>Mature Secondary Structure 
SQIASTTLKGQCIAEFLGTALLIFFGCGCVAALKLAGATLSLWEISVIWGLGVALAIYL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
TAAISGAHLNPAVTIALWLFACFERRKVLPYIIAQVAGAFCAAALVYAMYHNLFIDYEQT
HHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
HHMVRGSEESLTLAGIFSTYPNPHITVWQALLVETVITAILMCLILALTDDGNGIPRGPL
HHHHCCCCCCEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
APLLIGVLIAVIGAAMGPLTGFALNPARDFGPKAFAWMAGWGEIAFTGGRSIPYFLVPIV
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHCCCCCEEEECCCCCCHHHHHHH
GPIIGACLGAFGYRALIGRHLPCDVCIAEEEKKNSEKSKA
HHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]