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The map label for this gene is insB [H]

Identifier: 238921619

GI number: 238921619

Start: 3659324

End: 3659719

Strand: Reverse

Name: insB [H]

Synonym: NT01EI_3774

Alternate gene names: 238921619

Gene position: 3659719-3659324 (Counterclockwise)

Preceding gene: 238921620

Following gene: 238921618

Centisome position: 96.0

GC content: 49.49

Gene sequence:

>396_bases
GTGGGCAGTAAAGCCCGGCAGCACTGGCTCTGGTACGCGTACAACACCAAAACAGGCGGTGTACTGGCCTACACTTTTGG
TCCCAAAACCGATGAAAGCTGCCGGGAGCTACTGGTACTGATCACGCCATTCAACATTGGTATGATCACCAGCGACAACC
GGAGCAGCGATGGCCGGGAGGTGCCGAAGGATAAACATCTGACCGGCAAAATACTCACCCAACGCATTGTGCGTAATAAC
CTGACGCTTCGCACCCACATTAAGCGGCTTGCCCGTAAAACAATCTGCTTCTCACGCTCGGTGAGATCCACGAAAAAGTC
ATCGGAGCTTTTATCGAAAAACACATGTTCTACTGATTGGATGCATCATCAAAAAATTTATAGCTATATCGTTTGA

Upstream 100 bases:

>100_bases
GTTCGCACTTTAAAAACTCACGCCGAGGCGAATAACATCATCCCCGGCTGCTCATGAAGATGTGGCGCTTATCTACGAAC
TCGATGAGCAATGAAGCTTT

Downstream 100 bases:

>100_bases
CCATATTATTTTTAGGCTACTCGTGGCGACGTACTTTTCAGTATTTACTCTCGTCTGAGGCGCGCGTGAGGAGGGACATC
CTATGACATATTTTTCCACG

Product: iso-IS1 ORF2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 131; Mature: 130

Protein sequence:

>131_residues
MGSKARQHWLWYAYNTKTGGVLAYTFGPKTDESCRELLVLITPFNIGMITSDNRSSDGREVPKDKHLTGKILTQRIVRNN
LTLRTHIKRLARKTICFSRSVRSTKKSSELLSKNTCSTDWMHHQKIYSYIV

Sequences:

>Translated_131_residues
MGSKARQHWLWYAYNTKTGGVLAYTFGPKTDESCRELLVLITPFNIGMITSDNRSSDGREVPKDKHLTGKILTQRIVRNN
LTLRTHIKRLARKTICFSRSVRSTKKSSELLSKNTCSTDWMHHQKIYSYIV
>Mature_130_residues
GSKARQHWLWYAYNTKTGGVLAYTFGPKTDESCRELLVLITPFNIGMITSDNRSSDGREVPKDKHLTGKILTQRIVRNNL
TLRTHIKRLARKTICFSRSVRSTKKSSELLSKNTCSTDWMHHQKIYSYIV

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1789853, Length=101, Percent_Identity=38.6138613861386, Blast_Score=79, Evalue=1e-16,
Organism=Escherichia coli, GI1788203, Length=101, Percent_Identity=38.6138613861386, Blast_Score=79, Evalue=1e-16,
Organism=Escherichia coli, GI1786203, Length=101, Percent_Identity=38.6138613861386, Blast_Score=79, Evalue=1e-16,
Organism=Escherichia coli, GI1786470, Length=106, Percent_Identity=37.7358490566038, Blast_Score=78, Evalue=2e-16,
Organism=Escherichia coli, GI1786459, Length=106, Percent_Identity=37.7358490566038, Blast_Score=78, Evalue=2e-16,
Organism=Escherichia coli, GI1787223, Length=101, Percent_Identity=38.6138613861386, Blast_Score=77, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 15134; Mature: 15002

Theoretical pI: Translated: 10.76; Mature: 10.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSKARQHWLWYAYNTKTGGVLAYTFGPKTDESCRELLVLITPFNIGMITSDNRSSDGRE
CCCCCCCEEEEEEEECCCCCEEEEEECCCCCHHHHHHEEEECCCCCEEEECCCCCCCCCC
VPKDKHLTGKILTQRIVRNNLTLRTHIKRLARKTICFSRSVRSTKKSSELLSKNTCSTDW
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
MHHQKIYSYIV
HHHHHHHHHCC
>Mature Secondary Structure 
GSKARQHWLWYAYNTKTGGVLAYTFGPKTDESCRELLVLITPFNIGMITSDNRSSDGRE
CCCCCCEEEEEEEECCCCCEEEEEECCCCCHHHHHHEEEECCCCCEEEECCCCCCCCCC
VPKDKHLTGKILTQRIVRNNLTLRTHIKRLARKTICFSRSVRSTKKSSELLSKNTCSTDW
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
MHHQKIYSYIV
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6265806 [H]