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The map label for this gene is glgX [H]

Identifier: 238921523

GI number: 238921523

Start: 3565785

End: 3567764

Strand: Direct

Name: glgX [H]

Synonym: NT01EI_3675

Alternate gene names: 238921523

Gene position: 3565785-3567764 (Clockwise)

Preceding gene: 238921522

Following gene: 238921524

Centisome position: 93.53

GC content: 62.73

Gene sequence:

>1980_bases
ATGCTCAGGCCGGGTAAACCCTATCCGCTGGGCGCGCATCTGACGCCACAGGGGGTCAATTTCGCCATCTACTCGGCGCA
CGCGCAGCGCATAGAGCTGTGCCTGTCCGATAGGGACGGCCATCAACAGTCCTATGAGCTGCCAGCGCGCAGCGGCGATA
TTTGGCATGGCCTACTGCCCGGCGGACACGCCGGGCTGAAGTATGCCTACCGCGTATATGGCCCCTGGCAGCCGGGAAAA
GGGCTGCGTTTTAACCCGGCTAAGATGGTACTGGACCCCTATGCGCGGGCGCTGAGCCACACGCTGTCGGCGGACGACCG
CCTGTGCGGCGGCCTGAACGATCCGGACTGGCATGACAACCGTGACCTGCTGCCACGCTGCATCGTCAGCGACGAACAGT
ATGCCTGGCAGGAGGATGTGCCGCCCGATACGCCCTGGGGTAGCACGGTTATCTATGAGGCCCACGTCCGCGGCCTGACC
CGGCAGCACCCCGGTATCCCACCGGATCTGCGCGGCAGCTACGCAGCACTGGCCCACCCTGTCATGCTGGAGCATTACGC
CCGTCTGGGGATCACCGCCCTGGAGCTGCTTCCCATCCAGCTACATGCGGATGAGCCCCGTTTACAGCGACTGGGGCTAA
GCAACTACTGGGGGTACAACGTACTGGCCCCTTTCGCCATTGAACCGCGATACCACTCCGGGCGCCCCGGTACCACGCCA
CTGAGCGAATTCTGCGCTGCCGTCAATGCCCTGCACCGTGCGGGGATCGAGGTGATACTCGACGTGGTGTTTAACCACAC
CGCGGAGCTGGACGAACACGGGCCGCTGGTCTCGCTGCGCGGCATCGACAACCGCAGCTACTACTGGTGCAACGGGCAGG
GTGCATTGGAAAACTGGAGCGGCTGCGGCAATACCCTGCGACTGACCAAGACCCACAGCGTGCAGTGGGTCATGGATTGC
CTGCGCTACTGGGTGGAGACCTGCCACGTTGATGGGTTCCGCTTCGATCTGGGGACGGTACTGGGGCGGACGCCGGACTT
TGTCCGCCACTCCCCCCTGTTTATCGCCATCGCCCAGGATCCGGTGCTGGCGCGGGTTAAATTTATTGCAGAGCCCTGGG
ATATCGGTCCGAACGGCTACCAACTGGGTCATTTCCCCCAATACTTCGCCGAGTGGAACGATCGCTTCCGCGATGACATG
CGCCGTTTCTGGCTGCAGAGCGATCTGCCCAACGGTCTCTTTGCCACCCGTTTCGCCGCCTCCAGCGACCTGTTTCGTCA
TCACGGCCGCACGCCCTCCTCCAGTATTAACCATATTACCTGCCACGACGGATTTACCCTGCGCGACCTGCTCAGCTACA
GCCAGCGTCATAACCGGGCCAACGGGGAGAACAACCGCGACGGTAATACCCACAATTACAGCCACAATCATGGCGTAGAA
GGTATTGACGCCGAGGATGAGATCCACGCGCGGCGCCAGGCCAGCGCCCGGTCGCTGCTCACCACGCTGCTGCTGGCGCA
GGGGACGCCAATGCTGCTGGCCGGGGATGAGATCGGCCATAGCCAGCAGGGCAACAATAACGCCTACTGCCAGGATAACC
CTATCACCTGGCTCGACTGGAGCCAGGCCGACGAGGGGCTGCTGCAGTTTACCGCTCAGCTGATCCACCTGCGCCGCCGT
ATCGCTGCACTGACCGACAATCGCTGGTGGAACGAGAACGATGGCAGCGTACGCTGGCTGAATGCCGATGCCCAACCCAT
GACGGAGGCTCAGTGGCAGGCGGTACCCCCCGTAGCGCTGCAGATCCAGATGGCGGAGCAGTGGCTATTAGTGATCAACG
GCGGGCCACAGGCGCAGGCGATCGTCCTGCCGCCGGGCGACTGGCATATTCCGCCGCCGTTTGCCGTATTACGGGAAGAC
ATGCAGGGCGGATACCAGCATCTTGCGGCACGCAGCTGCTGTCTCTTTATGCGCATTTAG

Upstream 100 bases:

>100_bases
AGCGAGGCGGTGGGCAGCCACGGCTGTGAGTACTCCATCCAACTGACCCTGCCGCCGCTGTCAACGCTCTATCTGTGTCT
GGACAATGGGGACGGAGACG

Downstream 100 bases:

>100_bases
CGCGCTGACGCTGCGCCCCTGACGGTGTGAGCGGCCTTTTATAAGGAGTCGGAAATGGTGAAATCAGACAATCAAAATAA
ACTGATGTTGGCCCGCCAGC

Product: glycogen debranching enzyme

Products: NA

Alternate protein names: Glycogen operon protein GlgX [H]

Number of amino acids: Translated: 659; Mature: 659

Protein sequence:

>659_residues
MLRPGKPYPLGAHLTPQGVNFAIYSAHAQRIELCLSDRDGHQQSYELPARSGDIWHGLLPGGHAGLKYAYRVYGPWQPGK
GLRFNPAKMVLDPYARALSHTLSADDRLCGGLNDPDWHDNRDLLPRCIVSDEQYAWQEDVPPDTPWGSTVIYEAHVRGLT
RQHPGIPPDLRGSYAALAHPVMLEHYARLGITALELLPIQLHADEPRLQRLGLSNYWGYNVLAPFAIEPRYHSGRPGTTP
LSEFCAAVNALHRAGIEVILDVVFNHTAELDEHGPLVSLRGIDNRSYYWCNGQGALENWSGCGNTLRLTKTHSVQWVMDC
LRYWVETCHVDGFRFDLGTVLGRTPDFVRHSPLFIAIAQDPVLARVKFIAEPWDIGPNGYQLGHFPQYFAEWNDRFRDDM
RRFWLQSDLPNGLFATRFAASSDLFRHHGRTPSSSINHITCHDGFTLRDLLSYSQRHNRANGENNRDGNTHNYSHNHGVE
GIDAEDEIHARRQASARSLLTTLLLAQGTPMLLAGDEIGHSQQGNNNAYCQDNPITWLDWSQADEGLLQFTAQLIHLRRR
IAALTDNRWWNENDGSVRWLNADAQPMTEAQWQAVPPVALQIQMAEQWLLVINGGPQAQAIVLPPGDWHIPPPFAVLRED
MQGGYQHLAARSCCLFMRI

Sequences:

>Translated_659_residues
MLRPGKPYPLGAHLTPQGVNFAIYSAHAQRIELCLSDRDGHQQSYELPARSGDIWHGLLPGGHAGLKYAYRVYGPWQPGK
GLRFNPAKMVLDPYARALSHTLSADDRLCGGLNDPDWHDNRDLLPRCIVSDEQYAWQEDVPPDTPWGSTVIYEAHVRGLT
RQHPGIPPDLRGSYAALAHPVMLEHYARLGITALELLPIQLHADEPRLQRLGLSNYWGYNVLAPFAIEPRYHSGRPGTTP
LSEFCAAVNALHRAGIEVILDVVFNHTAELDEHGPLVSLRGIDNRSYYWCNGQGALENWSGCGNTLRLTKTHSVQWVMDC
LRYWVETCHVDGFRFDLGTVLGRTPDFVRHSPLFIAIAQDPVLARVKFIAEPWDIGPNGYQLGHFPQYFAEWNDRFRDDM
RRFWLQSDLPNGLFATRFAASSDLFRHHGRTPSSSINHITCHDGFTLRDLLSYSQRHNRANGENNRDGNTHNYSHNHGVE
GIDAEDEIHARRQASARSLLTTLLLAQGTPMLLAGDEIGHSQQGNNNAYCQDNPITWLDWSQADEGLLQFTAQLIHLRRR
IAALTDNRWWNENDGSVRWLNADAQPMTEAQWQAVPPVALQIQMAEQWLLVINGGPQAQAIVLPPGDWHIPPPFAVLRED
MQGGYQHLAARSCCLFMRI
>Mature_659_residues
MLRPGKPYPLGAHLTPQGVNFAIYSAHAQRIELCLSDRDGHQQSYELPARSGDIWHGLLPGGHAGLKYAYRVYGPWQPGK
GLRFNPAKMVLDPYARALSHTLSADDRLCGGLNDPDWHDNRDLLPRCIVSDEQYAWQEDVPPDTPWGSTVIYEAHVRGLT
RQHPGIPPDLRGSYAALAHPVMLEHYARLGITALELLPIQLHADEPRLQRLGLSNYWGYNVLAPFAIEPRYHSGRPGTTP
LSEFCAAVNALHRAGIEVILDVVFNHTAELDEHGPLVSLRGIDNRSYYWCNGQGALENWSGCGNTLRLTKTHSVQWVMDC
LRYWVETCHVDGFRFDLGTVLGRTPDFVRHSPLFIAIAQDPVLARVKFIAEPWDIGPNGYQLGHFPQYFAEWNDRFRDDM
RRFWLQSDLPNGLFATRFAASSDLFRHHGRTPSSSINHITCHDGFTLRDLLSYSQRHNRANGENNRDGNTHNYSHNHGVE
GIDAEDEIHARRQASARSLLTTLLLAQGTPMLLAGDEIGHSQQGNNNAYCQDNPITWLDWSQADEGLLQFTAQLIHLRRR
IAALTDNRWWNENDGSVRWLNADAQPMTEAQWQAVPPVALQIQMAEQWLLVINGGPQAQAIVLPPGDWHIPPPFAVLRED
MQGGYQHLAARSCCLFMRI

Specific function: Hydrolyzes the alpha-1,6-glucosidic linkages in glycogen which has first been partially depolymerized by phosphorylase. Shows only very little activity with native glycogen [H]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=657, Percent_Identity=58.751902587519, Blast_Score=796, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR022844
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 74348; Mature: 74348

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRPGKPYPLGAHLTPQGVNFAIYSAHAQRIELCLSDRDGHQQSYELPARSGDIWHGLLP
CCCCCCCCCCCCCCCCCCCCEEEEECHHHEEHHEECCCCCCCCCCCCCCCCCCCEECCCC
GGHAGLKYAYRVYGPWQPGKGLRFNPAKMVLDPYARALSHTLSADDRLCGGLNDPDWHDN
CCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCC
RDLLPRCIVSDEQYAWQEDVPPDTPWGSTVIYEAHVRGLTRQHPGIPPDLRGSYAALAHP
CCCCHHHHCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHCCCCCCCCCCCCHHHHHHH
VMLEHYARLGITALELLPIQLHADEPRLQRLGLSNYWGYNVLAPFAIEPRYHSGRPGTTP
HHHHHHHHHCCEEEEEEEEEEECCCHHHHHCCCCCCCCCCEEECEEECCCCCCCCCCCCC
LSEFCAAVNALHRAGIEVILDVVFNHTAELDEHGPLVSLRGIDNRSYYWCNGQGALENWS
HHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEEECCCCCEEEECCCCCCCCCC
GCGNTLRLTKTHSVQWVMDCLRYWVETCHVDGFRFDLGTVLGRTPDFVRHSPLFIAIAQD
CCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHCCCCHHHCCCCEEEEEECC
PVLARVKFIAEPWDIGPNGYQLGHFPQYFAEWNDRFRDDMRRFWLQSDLPNGLFATRFAA
CHHHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
SSDLFRHHGRTPSSSINHITCHDGFTLRDLLSYSQRHNRANGENNRDGNTHNYSHNHGVE
HHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
GIDAEDEIHARRQASARSLLTTLLLAQGTPMLLAGDEIGHSQQGNNNAYCQDNPITWLDW
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEC
SQADEGLLQFTAQLIHLRRRIAALTDNRWWNENDGSVRWLNADAQPMTEAQWQAVPPVAL
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEE
QIQMAEQWLLVINGGPQAQAIVLPPGDWHIPPPFAVLREDMQGGYQHLAARSCCLFMRI
EEEECCEEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEC
>Mature Secondary Structure
MLRPGKPYPLGAHLTPQGVNFAIYSAHAQRIELCLSDRDGHQQSYELPARSGDIWHGLLP
CCCCCCCCCCCCCCCCCCCCEEEEECHHHEEHHEECCCCCCCCCCCCCCCCCCCEECCCC
GGHAGLKYAYRVYGPWQPGKGLRFNPAKMVLDPYARALSHTLSADDRLCGGLNDPDWHDN
CCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCC
RDLLPRCIVSDEQYAWQEDVPPDTPWGSTVIYEAHVRGLTRQHPGIPPDLRGSYAALAHP
CCCCHHHHCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHCCCCCCCCCCCCHHHHHHH
VMLEHYARLGITALELLPIQLHADEPRLQRLGLSNYWGYNVLAPFAIEPRYHSGRPGTTP
HHHHHHHHHCCEEEEEEEEEEECCCHHHHHCCCCCCCCCCEEECEEECCCCCCCCCCCCC
LSEFCAAVNALHRAGIEVILDVVFNHTAELDEHGPLVSLRGIDNRSYYWCNGQGALENWS
HHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEEECCCCCEEEECCCCCCCCCC
GCGNTLRLTKTHSVQWVMDCLRYWVETCHVDGFRFDLGTVLGRTPDFVRHSPLFIAIAQD
CCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHCCCCHHHCCCCEEEEEECC
PVLARVKFIAEPWDIGPNGYQLGHFPQYFAEWNDRFRDDMRRFWLQSDLPNGLFATRFAA
CHHHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
SSDLFRHHGRTPSSSINHITCHDGFTLRDLLSYSQRHNRANGENNRDGNTHNYSHNHGVE
HHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
GIDAEDEIHARRQASARSLLTTLLLAQGTPMLLAGDEIGHSQQGNNNAYCQDNPITWLDW
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEC
SQADEGLLQFTAQLIHLRRRIAALTDNRWWNENDGSVRWLNADAQPMTEAQWQAVPPVAL
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCEEE
QIQMAEQWLLVINGGPQAQAIVLPPGDWHIPPPFAVLREDMQGGYQHLAARSCCLFMRI
EEEECCEEEEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA