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The map label for this gene is tnpR [H]

Identifier: 238921274

GI number: 238921274

Start: 3304876

End: 3305448

Strand: Direct

Name: tnpR [H]

Synonym: NT01EI_3417

Alternate gene names: 238921274

Gene position: 3304876-3305448 (Clockwise)

Preceding gene: 238921271

Following gene: 238921275

Centisome position: 86.69

GC content: 54.62

Gene sequence:

>573_bases
ATGACTGAAATACAGATTGGCTATGCCCGTTGCTCTACAGACAAGCAGGATTTGACTGCCCAGCAGGAAACCCTGGCGAA
ACTAGGTGTCTCCCCGGACCGGATTTATACCGATAAGGGACTGACCGGCTCAAACCGCCAGAGACCTGGTCTGGATCAGG
CGCTGGCCGCTGTACGCAGCGGTGACACGCTGGTTGTTCCCAAGCTTGACCGCCTGGCGCGCTCTGTGGCTGATGCGCGT
GAGATAGCAGATGCATTACAGGCCAGAGGCGTGAAGCTGGCACTGGGAACCCGCATTTACGATCCGACAGACCCGATGGG
GAAAATGTTCTTCAATGTGCTGGCGACATTTGCTGAGTTTGAAGATGGCCTGATACGTCTGCGTACCCGCGAAGGTATGG
CCATCGCCAGGGCAAAAGGGAAACTGCGGGGAAAACAGCCAAAATTGTCTGATAAACAGCAACAGGAGCTTTGCAGGATG
CACGGAAGTGGTGAGTACTCTATCAGCGATCTGGCTGAATTATTCTCCGTCTCCCGCCCCACGGTCTATCGGACGCTTTC
ACGTGGAAAATAA

Upstream 100 bases:

>100_bases
TAAAAAAGCATTGTGCGCTTCAGATAAAATCACTGCTGACCTTGTCTCATATAACTGGTACGGTATTGTTCTCTTTCATG
ATCCCCTAAACGTACACTTT

Downstream 100 bases:

>100_bases
TGGAAAACTGGATTTAATGAGAATTAACATGGAGCGGTTATGTACAACAATGAAATCATCAGTTACTTACAGGCCAACAA
AATTCTCGCATTAAAACTCG

Product: resolvase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 190; Mature: 189

Protein sequence:

>190_residues
MTEIQIGYARCSTDKQDLTAQQETLAKLGVSPDRIYTDKGLTGSNRQRPGLDQALAAVRSGDTLVVPKLDRLARSVADAR
EIADALQARGVKLALGTRIYDPTDPMGKMFFNVLATFAEFEDGLIRLRTREGMAIARAKGKLRGKQPKLSDKQQQELCRM
HGSGEYSISDLAELFSVSRPTVYRTLSRGK

Sequences:

>Translated_190_residues
MTEIQIGYARCSTDKQDLTAQQETLAKLGVSPDRIYTDKGLTGSNRQRPGLDQALAAVRSGDTLVVPKLDRLARSVADAR
EIADALQARGVKLALGTRIYDPTDPMGKMFFNVLATFAEFEDGLIRLRTREGMAIARAKGKLRGKQPKLSDKQQQELCRM
HGSGEYSISDLAELFSVSRPTVYRTLSRGK
>Mature_189_residues
TEIQIGYARCSTDKQDLTAQQETLAKLGVSPDRIYTDKGLTGSNRQRPGLDQALAAVRSGDTLVVPKLDRLARSVADARE
IADALQARGVKLALGTRIYDPTDPMGKMFFNVLATFAEFEDGLIRLRTREGMAIARAKGKLRGKQPKLSDKQQQELCRMH
GSGEYSISDLAELFSVSRPTVYRTLSRGK

Specific function: Resolvase catalyzes the resolution (a site-specific recombination) of the cointegrated replicon to yield the final transposition products [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=184, Percent_Identity=36.4130434782609, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1787827, Length=191, Percent_Identity=36.6492146596859, Blast_Score=97, Evalue=5e-22,
Organism=Escherichia coli, GI1787638, Length=186, Percent_Identity=36.5591397849462, Blast_Score=97, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 20962; Mature: 20831

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEIQIGYARCSTDKQDLTAQQETLAKLGVSPDRIYTDKGLTGSNRQRPGLDQALAAVRS
CCCEEEEHHHCCCCHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHC
GDTLVVPKLDRLARSVADAREIADALQARGVKLALGTRIYDPTDPMGKMFFNVLATFAEF
CCEEECCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
EDGLIRLRTREGMAIARAKGKLRGKQPKLSDKQQQELCRMHGSGEYSISDLAELFSVSRP
HCCEEEEECCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCC
TVYRTLSRGK
HHHHHHHCCC
>Mature Secondary Structure 
TEIQIGYARCSTDKQDLTAQQETLAKLGVSPDRIYTDKGLTGSNRQRPGLDQALAAVRS
CCEEEEHHHCCCCHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHC
GDTLVVPKLDRLARSVADAREIADALQARGVKLALGTRIYDPTDPMGKMFFNVLATFAEF
CCEEECCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
EDGLIRLRTREGMAIARAKGKLRGKQPKLSDKQQQELCRMHGSGEYSISDLAELFSVSRP
HCCEEEEECCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCC
TVYRTLSRGK
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2170815; 2555186 [H]