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The map label for this gene is pnuC [H]

Identifier: 238921252

GI number: 238921252

Start: 3280911

End: 3281639

Strand: Direct

Name: pnuC [H]

Synonym: NT01EI_3395

Alternate gene names: 238921252

Gene position: 3280911-3281639 (Clockwise)

Preceding gene: 238921251

Following gene: 238921255

Centisome position: 86.06

GC content: 59.53

Gene sequence:

>729_bases
ATGGATTTTTTCAGCATTAACAACGTAATGGCAAACATTCCCCTCGGCGAAGGCGGATATGCCCTGTCATGGATCGAAGC
CATCGGCACGCTGTTTGGCCTGCTGTGTATCTGGTTTGCCAGCAAAGAGAAGATCATCAACTACCTGTTTGGGCTGATCA
ACGTCACCCTGTTTGCCGCCATCTTTTTCCAAATCCAGCTGTACGCCAGCCTGCTGCTACAGGTGTTCTTTTTCGCCGCC
AATCTGTACGGCTGGTACGCCTGGAGCCGGCAGAACGGCGATAACGAGGCAGCTCTCAAGGTGCGTTGGCTGTCGCGTGG
CAAAGCGCTGACCCTGACCCTGGTCTGTCTGGTCGCCATTGCCCTGATGACCCGCTTTATCGATCCGGTGTTCGCCCTCC
TTACCCGCGTCGCCATCGACCTGCTGCAGGGGATGGGCGTGGCCGCCGTGATGCCGGATCTGCAGCCGGACGCCTTCCCG
CTGTGGGACTCCGCCATGCTGGTGCTCTCCATCGCCGCCATGGTGCTGATGACCCGCAAATACGTTGAGAACTGGCTGCT
GTGGGTGGTGATCGACATCATCAGCGTGGTGATTTACGCCATCCAGGGGGTCTACGCCATGTCGCTGGAGTACATCCTGC
TGACCGCGATCGCCGTGATGGGCTCCGTGACCTGGATCAACAGCGCACGCCGCCACGGCTCCCGCCCGCTGGCACCGGCC
ACCGTATAA

Upstream 100 bases:

>100_bases
ACTACCAGCAGCAGGAAGCGCACAGCCAGCGTGGCGAAGCCAGGGAGATCGAGCTGGCCCTCAACGCCATCCGACAGGAT
CATAACAAAGGGAACTGAAC

Downstream 100 bases:

>100_bases
TCGCCCTGTCCGCGTCCCGCCAGCCGGGACGCCCCTCTGCGGCGCGCCGCCCCGTCGCGCGGCTCATCTCACAACGGTAG
ACGGTAGGTCACCTGCCGCG

Product: intergral membrane NMN transport protein PnuC

Products: nicotinamide nucleotide [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MDFFSINNVMANIPLGEGGYALSWIEAIGTLFGLLCIWFASKEKIINYLFGLINVTLFAAIFFQIQLYASLLLQVFFFAA
NLYGWYAWSRQNGDNEAALKVRWLSRGKALTLTLVCLVAIALMTRFIDPVFALLTRVAIDLLQGMGVAAVMPDLQPDAFP
LWDSAMLVLSIAAMVLMTRKYVENWLLWVVIDIISVVIYAIQGVYAMSLEYILLTAIAVMGSVTWINSARRHGSRPLAPA
TV

Sequences:

>Translated_242_residues
MDFFSINNVMANIPLGEGGYALSWIEAIGTLFGLLCIWFASKEKIINYLFGLINVTLFAAIFFQIQLYASLLLQVFFFAA
NLYGWYAWSRQNGDNEAALKVRWLSRGKALTLTLVCLVAIALMTRFIDPVFALLTRVAIDLLQGMGVAAVMPDLQPDAFP
LWDSAMLVLSIAAMVLMTRKYVENWLLWVVIDIISVVIYAIQGVYAMSLEYILLTAIAVMGSVTWINSARRHGSRPLAPA
TV
>Mature_242_residues
MDFFSINNVMANIPLGEGGYALSWIEAIGTLFGLLCIWFASKEKIINYLFGLINVTLFAAIFFQIQLYASLLLQVFFFAA
NLYGWYAWSRQNGDNEAALKVRWLSRGKALTLTLVCLVAIALMTRFIDPVFALLTRVAIDLLQGMGVAAVMPDLQPDAFP
LWDSAMLVLSIAAMVLMTRKYVENWLLWVVIDIISVVIYAIQGVYAMSLEYILLTAIAVMGSVTWINSARRHGSRPLAPA
TV

Specific function: Required for nicotinamide riboside transport across the inner membrane [H]

COG id: COG3201

COG function: function code H; Nicotinamide mononucleotide transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nicotinamide ribonucleoside (NR) uptake permease (TC 4.B.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786965, Length=238, Percent_Identity=71.4285714285714, Blast_Score=335, Evalue=2e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006419 [H]

Pfam domain/function: PF04973 NMN_transporter [H]

EC number: NA

Molecular weight: Translated: 26944; Mature: 26944

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CC
>Mature Secondary Structure
MDFFSINNVMANIPLGEGGYALSWIEAIGTLFGLLCIWFASKEKIINYLFGLINVTLFAA
CCCCHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IFFQIQLYASLLLQVFFFAANLYGWYAWSRQNGDNEAALKVRWLSRGKALTLTLVCLVAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEEEHHCCCHHHHHHHHHHHH
ALMTRFIDPVFALLTRVAIDLLQGMGVAAVMPDLQPDAFPLWDSAMLVLSIAAMVLMTRK
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
YVENWLLWVVIDIISVVIYAIQGVYAMSLEYILLTAIAVMGSVTWINSARRHGSRPLAPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
TV
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nicotinamide nucleotide [Periplasm] [C]

Specific reaction: nicotinamide nucleotide [Periplasm] = nicotinamide nucleotide [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2198247; 11677609 [H]