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The map label for this gene is rpoS [H]

Identifier: 238921111

GI number: 238921111

Start: 3128438

End: 3129424

Strand: Reverse

Name: rpoS [H]

Synonym: NT01EI_3250

Alternate gene names: 238921111

Gene position: 3129424-3128438 (Counterclockwise)

Preceding gene: 238921112

Following gene: 238921106

Centisome position: 82.09

GC content: 57.55

Gene sequence:

>987_bases
ATGAGTCATACTACGCTGAAAGTTAACGAATTACATGATGAAGTCGAGTTGGAGGATGGCGTTGAGGACGGTTTTGACGA
GAAGGACCTCGTTGAAGAGTCCATGGAAAATGACATCACCGATGATGAATTGCTGTCTCAGTCTGTTTCACAGCGCGTTC
TGGACGCCACGCAGCTTTATCTTAGCGAAATCGGTTATGCGCCGCTGTTGACGGCGGAAGAGGAGGTTTTCTTTGCCCGC
CGGGCCCTGCGTGGGGATGAGGCATCGCGTAAGCGGATGATCGAAAGCAACCTGCGACTGGTGGTCAAGATCTCCCGCCG
CTACAGCAATCGCGGTCTGGCACTGCTGGATCTTATCGAAGAGGGTAATCTGGGTCTGATCCGCGCGGTAGAGAAGTTTG
ACCCGGAGCGCGGCTTCCGTTTTTCGACCTACGCTACCTGGTGGATCCGTCAGACCATTGAGCGGGCCATCATGAACCAG
ACCCGCACCATCCGTCTGCCTATCCATATCGTTAAAGAGCTTAACGTCTATCTGCGCACCGCCCGCGAACTCGCTCATAA
GCTGGACCATGAGCCGAGCGCGGAGGAGATCGCCGAGCGGCTGGAGAAGCCGGTGCATGATGTCAGCCGTATGCTGCGTC
TCAACGAACGGATCACCTCAGTGGATACCCCACTGGGCGGCGATTCGGAGAAGGCGCTGCTGGATATTCTGACTGATGAG
AACGAAAGCGGGCCGGAGGATTGCACCCAGAACGATGACATGAAGCAGAGCATCGTCAATTGGCTGTTTGAGCTGAACGC
CAAGCAGCGCGAGGTGCTGGCGCGCCGCTTTGGCCTGCTGGGCTATGAGGCCGCCACGCTGGAGGACGTTGGGCGAGAGA
TTGGCCTGACCCGCGAGCGGGTGCGTCAAATCCAGGTTGAAGGGCTGCGGCGCCTGCGAGAGATCCTGCAGATGCAGGGA
CTGAGTATCGAGGCCCTGTTCCGCTGA

Upstream 100 bases:

>100_bases
TTACAAGGGAAAATCCGTAAACCCGCTGCAATATCTGCCGCAGCGATAGTTTGAGCAGGATCGCTGTATCCTGCTCGCAA
ATCACGGGTAGGGTACAGCT

Downstream 100 bases:

>100_bases
GCGGCGTGGTCAAAAAAACGGTGAAGCCAAGGCTTCACCGTTTTTTTATCGCTATAGCATCCCCTTGAGGCGATAGAGCC
AGTCCAGCGCCTGGCGCGGC

Product: RNA polymerase sigma factor RpoS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MSHTTLKVNELHDEVELEDGVEDGFDEKDLVEESMENDITDDELLSQSVSQRVLDATQLYLSEIGYAPLLTAEEEVFFAR
RALRGDEASRKRMIESNLRLVVKISRRYSNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQ
TRTIRLPIHIVKELNVYLRTARELAHKLDHEPSAEEIAERLEKPVHDVSRMLRLNERITSVDTPLGGDSEKALLDILTDE
NESGPEDCTQNDDMKQSIVNWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQMQG
LSIEALFR

Sequences:

>Translated_328_residues
MSHTTLKVNELHDEVELEDGVEDGFDEKDLVEESMENDITDDELLSQSVSQRVLDATQLYLSEIGYAPLLTAEEEVFFAR
RALRGDEASRKRMIESNLRLVVKISRRYSNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQ
TRTIRLPIHIVKELNVYLRTARELAHKLDHEPSAEEIAERLEKPVHDVSRMLRLNERITSVDTPLGGDSEKALLDILTDE
NESGPEDCTQNDDMKQSIVNWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQMQG
LSIEALFR
>Mature_327_residues
SHTTLKVNELHDEVELEDGVEDGFDEKDLVEESMENDITDDELLSQSVSQRVLDATQLYLSEIGYAPLLTAEEEVFFARR
ALRGDEASRKRMIESNLRLVVKISRRYSNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQT
RTIRLPIHIVKELNVYLRTARELAHKLDHEPSAEEIAERLEKPVHDVSRMLRLNERITSVDTPLGGDSEKALLDILTDEN
ESGPEDCTQNDDMKQSIVNWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQMQGL
SIEALFR

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is required for the normal expression of the enterotoxin, yst, in the stationary phase [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=330, Percent_Identity=86.6666666666667, Blast_Score=561, Evalue=1e-161,
Organism=Escherichia coli, GI1789448, Length=268, Percent_Identity=41.044776119403, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1789871, Length=270, Percent_Identity=26.2962962962963, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 37889; Mature: 37758

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHTTLKVNELHDEVELEDGVEDGFDEKDLVEESMENDITDDELLSQSVSQRVLDATQLY
CCCCEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
LSEIGYAPLLTAEEEVFFARRALRGDEASRKRMIESNLRLVVKISRRYSNRGLALLDLIE
HHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEHHHHCCCCCHHHHHHH
EGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIVKELNVYLRT
CCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHH
ARELAHKLDHEPSAEEIAERLEKPVHDVSRMLRLNERITSVDTPLGGDSEKALLDILTDE
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCC
NESGPEDCTQNDDMKQSIVNWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRER
CCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHH
VRQIQVEGLRRLREILQMQGLSIEALFR
HHHHHHHHHHHHHHHHHHCCCCHHHHCC
>Mature Secondary Structure 
SHTTLKVNELHDEVELEDGVEDGFDEKDLVEESMENDITDDELLSQSVSQRVLDATQLY
CCCEEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
LSEIGYAPLLTAEEEVFFARRALRGDEASRKRMIESNLRLVVKISRRYSNRGLALLDLIE
HHHCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEHHHHCCCCCHHHHHHH
EGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHIVKELNVYLRT
CCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHH
ARELAHKLDHEPSAEEIAERLEKPVHDVSRMLRLNERITSVDTPLGGDSEKALLDILTDE
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCC
NESGPEDCTQNDDMKQSIVNWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRER
CCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHH
VRQIQVEGLRRLREILQMQGLSIEALFR
HHHHHHHHHHHHHHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7729893 [H]