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The map label for this gene is gshA

Identifier: 238921093

GI number: 238921093

Start: 3104923

End: 3106482

Strand: Reverse

Name: gshA

Synonym: NT01EI_3229

Alternate gene names: 238921093

Gene position: 3106482-3104923 (Counterclockwise)

Preceding gene: 238921094

Following gene: 238921092

Centisome position: 81.49

GC content: 59.81

Gene sequence:

>1560_bases
TTGATCCCGGACGTATCTCAGGCCCTTAGCTGGCTGGAATCTCATCCGAATGCCCTGGATGGCATCAGGCGCGGTATTGA
GCGCGAAACGCTGCGTGTCACTCCCGAGGGGCATTTGGCTACTACGCCGCATCCGGCGGTGTTGGGCAAGGCGTTGACCC
ACCCCTGGATTACTACTGACTTCGCCGAAAGCCTGCTGGAGTTTATCACGCCTGTCGATGGCAGCATCGATCATATGCTG
ACGTTCCTCAGTGATATTCACCGCTATGTGGCGCGCAATCTCGGCGATGAGCGTATGTGGCCCCTGAGCATGCCCTGTTT
TATCGGGAGCGAGCAGGATATCATCCTGGCCCAGTACGGCAGCAGTAACTTGGGGCGCTTTAAAACGCTCTACCGCGAGG
GGCTGAAGAATCGTTATGGGGCGCTGATGCAGACCATCTCCGGCGTGCACTATAACTTCTCCCTGCCGCTGTCGTTTTGG
CAGGCGCGCGCCGGTGTGCAGGATGCGGATAGCGGTAAGGCTGCCATTTCTGACGGTTATTTCCGCCTGATCCGGAACTA
CTATCGCTTTGGCTGGGTGATCCCCTATCTGTTTGGTGCTTCACCGGCTATCTGTAGCTCCTTCCTGAACGGGCGGGAGA
GCGCGCTGCCGTTTGAGCGCCGCGGCGGAACGCTGTTCCTGCCCTATGCTACCTCTCTGCGCCTGAGCGATCTGGGATAC
ACCAATAAGTCGCAGAGTAATCTTGGGATCACGTTTAATCACCTTAATCAGTATGTCGAAGGGCTGAAACGTGCTATTCA
CACCCCCTCCGCTGAGTTTGCCCGCCTGGGCGTGGAGGAAAGCGGCCACTATCACCAGCTGAATGCCAATATTTTGCAGA
TCGAGAATGAGCTCTATGCGCCGATCCGACCCAAGCGTGTCACACGTGACGGTGAGTCACCATCTGATGCGCTGCTGCGC
GGCGGCGTAGAGTACATTGAGGTTCGCTCTCTGGACATTAACCCGTTCACCCCCATCGGCGTCGATGCCGATCAGGCCCG
CTTCCTCGATCTGTTCCTGATCTGGTGCGTGCTGGCCGACGCGCCAGAGATGAGCAGTGACGAGCTGCTGTGTACGCGCC
AAAACTGGAACCGGGTGATCCTTGAGGGGCGTAAGCCGGGGCAGACCATCGGCATTGGCTGTCATGATGCCCGTCAGCCG
CTGGCGTACGTGGGGCAGTCACTCTTTGCCGACCTGCATCGGGTCGCCGAGGTGCTGGATGGCATTAACGGTGATGCGCG
CTACCAGCAGGTCTGCGCTCGCCTGGTCGCGGCCTTCGAGGACGTTTCACTGACTTACTCGGCACGGGTCGTAGCCCAGA
TGGGGGAGCGCGGGATCGGCGGTTATGGCCTGACGCTGGCGCAGCATTACCGCGATCGGCTGTGCAGCGAACCGCTTGCG
TTGTTGGATGAGACAACGCTGGCGGTACAGGCGCAGCGCTCGGTTACCCGCCAGGCGGCGCTGGAGTCTCAGAGCTGCGA
GTCCTTTGCGTCCTACCTCCGGCAGCACGCCGGAGGCTAA

Upstream 100 bases:

>100_bases
GCATGGTTGACGCTGTACGGCATGTCGTGGTGGCATTAGGCGTCCATTGCGGTAAGACGCGGTGGGCGCGATTAACTCTT
CTTATTAGCGGGAGGTCAAT

Downstream 100 bases:

>100_bases
AAAAGAAAAAGGCCACAATATCTGTGGCCAAAATAAGCATCTCTGAGGACAGGGATGATGATAACGAAATATGGAAGACG
CGCATGTGTGTCTTTCATAT

Product: glutamate-cysteine ligase

Products: NA

Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MIPDVSQALSWLESHPNALDGIRRGIERETLRVTPEGHLATTPHPAVLGKALTHPWITTDFAESLLEFITPVDGSIDHML
TFLSDIHRYVARNLGDERMWPLSMPCFIGSEQDIILAQYGSSNLGRFKTLYREGLKNRYGALMQTISGVHYNFSLPLSFW
QARAGVQDADSGKAAISDGYFRLIRNYYRFGWVIPYLFGASPAICSSFLNGRESALPFERRGGTLFLPYATSLRLSDLGY
TNKSQSNLGITFNHLNQYVEGLKRAIHTPSAEFARLGVEESGHYHQLNANILQIENELYAPIRPKRVTRDGESPSDALLR
GGVEYIEVRSLDINPFTPIGVDADQARFLDLFLIWCVLADAPEMSSDELLCTRQNWNRVILEGRKPGQTIGIGCHDARQP
LAYVGQSLFADLHRVAEVLDGINGDARYQQVCARLVAAFEDVSLTYSARVVAQMGERGIGGYGLTLAQHYRDRLCSEPLA
LLDETTLAVQAQRSVTRQAALESQSCESFASYLRQHAGG

Sequences:

>Translated_519_residues
MIPDVSQALSWLESHPNALDGIRRGIERETLRVTPEGHLATTPHPAVLGKALTHPWITTDFAESLLEFITPVDGSIDHML
TFLSDIHRYVARNLGDERMWPLSMPCFIGSEQDIILAQYGSSNLGRFKTLYREGLKNRYGALMQTISGVHYNFSLPLSFW
QARAGVQDADSGKAAISDGYFRLIRNYYRFGWVIPYLFGASPAICSSFLNGRESALPFERRGGTLFLPYATSLRLSDLGY
TNKSQSNLGITFNHLNQYVEGLKRAIHTPSAEFARLGVEESGHYHQLNANILQIENELYAPIRPKRVTRDGESPSDALLR
GGVEYIEVRSLDINPFTPIGVDADQARFLDLFLIWCVLADAPEMSSDELLCTRQNWNRVILEGRKPGQTIGIGCHDARQP
LAYVGQSLFADLHRVAEVLDGINGDARYQQVCARLVAAFEDVSLTYSARVVAQMGERGIGGYGLTLAQHYRDRLCSEPLA
LLDETTLAVQAQRSVTRQAALESQSCESFASYLRQHAGG
>Mature_519_residues
MIPDVSQALSWLESHPNALDGIRRGIERETLRVTPEGHLATTPHPAVLGKALTHPWITTDFAESLLEFITPVDGSIDHML
TFLSDIHRYVARNLGDERMWPLSMPCFIGSEQDIILAQYGSSNLGRFKTLYREGLKNRYGALMQTISGVHYNFSLPLSFW
QARAGVQDADSGKAAISDGYFRLIRNYYRFGWVIPYLFGASPAICSSFLNGRESALPFERRGGTLFLPYATSLRLSDLGY
TNKSQSNLGITFNHLNQYVEGLKRAIHTPSAEFARLGVEESGHYHQLNANILQIENELYAPIRPKRVTRDGESPSDALLR
GGVEYIEVRSLDINPFTPIGVDADQARFLDLFLIWCVLADAPEMSSDELLCTRQNWNRVILEGRKPGQTIGIGCHDARQP
LAYVGQSLFADLHRVAEVLDGINGDARYQQVCARLVAAFEDVSLTYSARVVAQMGERGIGGYGLTLAQHYRDRLCSEPLA
LLDETTLAVQAQRSVTRQAALESQSCESFASYLRQHAGG

Specific function: Glutathione biosynthesis; first step. [C]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1789044, Length=518, Percent_Identity=69.4980694980695, Blast_Score=758, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSH1_EDWI9 (C5BGG7)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002934608.1
- GeneID:   7961065
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_3229
- OMA:   FGLIRNY
- ProtClustDB:   CLSK2813583
- HAMAP:   MF_00578
- InterPro:   IPR007370
- InterPro:   IPR006334
- TIGRFAMs:   TIGR01434

Pfam domain/function: PF04262 Glu_cys_ligase

EC number: =6.3.2.2

Molecular weight: Translated: 57762; Mature: 57762

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPDVSQALSWLESHPNALDGIRRGIERETLRVTPEGHLATTPHPAVLGKALTHPWITTD
CCCCHHHHHHHHHHCCCHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHCCCCCCHH
FAESLLEFITPVDGSIDHMLTFLSDIHRYVARNLGDERMWPLSMPCFIGSEQDIILAQYG
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCEEEEECC
SSNLGRFKTLYREGLKNRYGALMQTISGVHYNFSLPLSFWQARAGVQDADSGKAAISDGY
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHCCCCCCCCCCHHHHHHH
FRLIRNYYRFGWVIPYLFGASPAICSSFLNGRESALPFERRGGTLFLPYATSLRLSDLGY
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHCCCHHHCCCEEEEECCCCEEEHHCCC
TNKSQSNLGITFNHLNQYVEGLKRAIHTPSAEFARLGVEESGHYHQLNANILQIENELYA
CCCCCCCCCEEHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCEEEECCCEEEECHHHCC
PIRPKRVTRDGESPSDALLRGGVEYIEVRSLDINPFTPIGVDADQARFLDLFLIWCVLAD
CCCCCCCCCCCCCCHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHC
APEMSSDELLCTRQNWNRVILEGRKPGQTIGIGCHDARQPLAYVGQSLFADLHRVAEVLD
CCCCCCCCEEEECCCCCEEEEECCCCCCEEECCCHHHCCHHHHHHHHHHHHHHHHHHHHC
GINGDARYQQVCARLVAAFEDVSLTYSARVVAQMGERGIGGYGLTLAQHYRDRLCSEPLA
CCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHH
LLDETTLAVQAQRSVTRQAALESQSCESFASYLRQHAGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIPDVSQALSWLESHPNALDGIRRGIERETLRVTPEGHLATTPHPAVLGKALTHPWITTD
CCCCHHHHHHHHHHCCCHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHCCCCCCHH
FAESLLEFITPVDGSIDHMLTFLSDIHRYVARNLGDERMWPLSMPCFIGSEQDIILAQYG
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCEEEEECC
SSNLGRFKTLYREGLKNRYGALMQTISGVHYNFSLPLSFWQARAGVQDADSGKAAISDGY
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHCCCCCCCCCCHHHHHHH
FRLIRNYYRFGWVIPYLFGASPAICSSFLNGRESALPFERRGGTLFLPYATSLRLSDLGY
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHCCCHHHCCCEEEEECCCCEEEHHCCC
TNKSQSNLGITFNHLNQYVEGLKRAIHTPSAEFARLGVEESGHYHQLNANILQIENELYA
CCCCCCCCCEEHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCEEEECCCEEEECHHHCC
PIRPKRVTRDGESPSDALLRGGVEYIEVRSLDINPFTPIGVDADQARFLDLFLIWCVLAD
CCCCCCCCCCCCCCHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHC
APEMSSDELLCTRQNWNRVILEGRKPGQTIGIGCHDARQPLAYVGQSLFADLHRVAEVLD
CCCCCCCCEEEECCCCCEEEEECCCCCCEEECCCHHHCCHHHHHHHHHHHHHHHHHHHHC
GINGDARYQQVCARLVAAFEDVSLTYSARVVAQMGERGIGGYGLTLAQHYRDRLCSEPLA
CCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHH
LLDETTLAVQAQRSVTRQAALESQSCESFASYLRQHAGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA