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The map label for this gene is trmJ [H]

Identifier: 238921055

GI number: 238921055

Start: 3064719

End: 3065456

Strand: Reverse

Name: trmJ [H]

Synonym: NT01EI_3185

Alternate gene names: 238921055

Gene position: 3065456-3064719 (Counterclockwise)

Preceding gene: 238921058

Following gene: 238921054

Centisome position: 80.41

GC content: 60.57

Gene sequence:

>738_bases
ATGCTGGACAATATTCGCATCGTGCTGGTGGAAACCTCCCACACCGGCAACATGGGGTCAACTGCCCGAGCCATGAAGAC
CATGGGATTAACCAACCTGTATCTTGTGAACCCGCTGGTACGCCCCGACTCACAGGCCATTGCTCTGTCAGCCGGGGCCA
GCGACGTGATCGGCAACGCGACCATCGTAGGCAGCTTCGATGACGCGATCGTCGGTTGCAGTCTGGTGGTGGGCACCAGT
GCCCGTTCACGCTCGTTGCCATGGCCGATGCTGGAGCCGCGCGAATGCGGCCAGCGCAGCGCCATGGAATCAGAGCATGC
GCCTGTTGCCATCGTATTTGGCCGCGAGCGCGTCGGGTTGACCAACGATGAGCTGCAGAAGTGCCATTACCATGTGCAGA
TCCCGGCCAACCCGGAGTACACCTCGCTGAACCTGGCGATGGCGGTGCAGATCCTCAGTTATGAAATCCGCGTCGCACAC
CTGGATCGTCAGGCGGCCAAGGCGCCGGCTGTGGAGGAGGCAGCCTATCCTCTGGCAGACGATCTGGAGCGCTTTTATGC
CCATCTGGAGCAGACGCTGCATCACACCGGCTTTATCCGTGAGAAGAGTCCGGGTCAGGTTATGAACCGTCTGCGCCGCC
TGTTCACCCGCGCCCGCCCGGAGGCGCAGGAGCTGAATATCCTGCGCGGCATACTGGCCTCAATTGATAAGTGCGACGCA
GAACGGCACAAAAGTTAA

Upstream 100 bases:

>100_bases
TTGATTGTGGAATTGCTCGGCGGTGAATAAATCAATAGAAACCATTAATAACGCCTTATGGTAAAATCACAGAATCAATT
CAATCGTGTAATGAGTAAGC

Downstream 100 bases:

>100_bases
CGGTGACAACGACTATATCATAGTAGAATACACGGGTATTTTTGCGCGGATGCTGGCTGGTATTGCTGCGTGTTTCTCGC
GGATGGACAGCATTGATAAG

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MLDNIRIVLVETSHTGNMGSTARAMKTMGLTNLYLVNPLVRPDSQAIALSAGASDVIGNATIVGSFDDAIVGCSLVVGTS
ARSRSLPWPMLEPRECGQRSAMESEHAPVAIVFGRERVGLTNDELQKCHYHVQIPANPEYTSLNLAMAVQILSYEIRVAH
LDRQAAKAPAVEEAAYPLADDLERFYAHLEQTLHHTGFIREKSPGQVMNRLRRLFTRARPEAQELNILRGILASIDKCDA
ERHKS

Sequences:

>Translated_245_residues
MLDNIRIVLVETSHTGNMGSTARAMKTMGLTNLYLVNPLVRPDSQAIALSAGASDVIGNATIVGSFDDAIVGCSLVVGTS
ARSRSLPWPMLEPRECGQRSAMESEHAPVAIVFGRERVGLTNDELQKCHYHVQIPANPEYTSLNLAMAVQILSYEIRVAH
LDRQAAKAPAVEEAAYPLADDLERFYAHLEQTLHHTGFIREKSPGQVMNRLRRLFTRARPEAQELNILRGILASIDKCDA
ERHKS
>Mature_245_residues
MLDNIRIVLVETSHTGNMGSTARAMKTMGLTNLYLVNPLVRPDSQAIALSAGASDVIGNATIVGSFDDAIVGCSLVVGTS
ARSRSLPWPMLEPRECGQRSAMESEHAPVAIVFGRERVGLTNDELQKCHYHVQIPANPEYTSLNLAMAVQILSYEIRVAH
LDRQAAKAPAVEEAAYPLADDLERFYAHLEQTLHHTGFIREKSPGQVMNRLRRLFTRARPEAQELNILRGILASIDKCDA
ERHKS

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=238, Percent_Identity=76.890756302521, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1790865, Length=173, Percent_Identity=34.1040462427746, Blast_Score=84, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27010; Mature: 27010

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDNIRIVLVETSHTGNMGSTARAMKTMGLTNLYLVNPLVRPDSQAIALSAGASDVIGNA
CCCCEEEEEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCCHHCCCE
TIVGSFDDAIVGCSLVVGTSARSRSLPWPMLEPRECGQRSAMESEHAPVAIVFGRERVGL
EEEECCCHHHHHHEEEEECCCCCCCCCCCCCCHHHHCHHHHCCCCCCCEEEEECCCCCCC
TNDELQKCHYHVQIPANPEYTSLNLAMAVQILSYEIRVAHLDRQAAKAPAVEEAAYPLAD
CHHHHHHCEEEEECCCCCCCCCHHHHHHHHHHHHHEEEEECCHHHHCCCCHHHHCCCCHH
DLERFYAHLEQTLHHTGFIREKSPGQVMNRLRRLFTRARPEAQELNILRGILASIDKCDA
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
ERHKS
HHCCC
>Mature Secondary Structure
MLDNIRIVLVETSHTGNMGSTARAMKTMGLTNLYLVNPLVRPDSQAIALSAGASDVIGNA
CCCCEEEEEEEECCCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCCHHCCCE
TIVGSFDDAIVGCSLVVGTSARSRSLPWPMLEPRECGQRSAMESEHAPVAIVFGRERVGL
EEEECCCHHHHHHEEEEECCCCCCCCCCCCCCHHHHCHHHHCCCCCCCEEEEECCCCCCC
TNDELQKCHYHVQIPANPEYTSLNLAMAVQILSYEIRVAHLDRQAAKAPAVEEAAYPLAD
CHHHHHHCEEEEECCCCCCCCCHHHHHHHHHHHHHEEEEECCHHHHCCCCHHHHCCCCHH
DLERFYAHLEQTLHHTGFIREKSPGQVMNRLRRLFTRARPEAQELNILRGILASIDKCDA
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
ERHKS
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA