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The map label for this gene is nagE [H]

Identifier: 238920788

GI number: 238920788

Start: 2799511

End: 2801526

Strand: Reverse

Name: nagE [H]

Synonym: NT01EI_2907

Alternate gene names: 238920788

Gene position: 2801526-2799511 (Counterclockwise)

Preceding gene: 238920792

Following gene: 238920787

Centisome position: 73.49

GC content: 58.28

Gene sequence:

>2016_bases
GTGAGTATTCTCGGTTATTTGCAAAAGGTAGGCCGCGCGCTGATGGTGCCGGTAGCGACGCTACCGGCCGCAGCAATACT
GATGGGTGTCGGTTACTGGATCGACCCGGTCGGCTGGGGGGGAGATAACGCACTGGCGGCGTTCTTCATCAAGTCCGGTT
CAGCCATCATCGACAATATGTCGGTGCTGTTTGCCATTGGTGTTGCATATGGTATGTCCAAAGATAAAGACGGCGCGGCG
GCACTGGCCGGTTTTGTCGGGTTCCTGGTGGTTACAACCCTGTGTTCGCCGGCTGCGGTGGCGATGATCCAGAAGATTCC
GGTGGATCAGGTTCCTGCCGCCTTCGGTAAGATCAATAACCAGTTTATCGGTATTCTGGTCGGTATTATCTCCGCTGAGT
TGTATAACCGTTACAGCAGCGTTGAGCTGCCCAAGGCGCTCTCCTTCTTCAGCGGCCGTCGTCTGGTACCGATCCTGACC
TCGTTCCTGATGATTATTCTGGCCTTTATCCTGATGTATATCTGGCCGGTAATCTTTAATGGCCTGGTGAACTTCGGTGA
GCATATCCAGAAGCTGGGCTCCGTGGGGGCGGGCGTGTATGCCTTCTTCAACCGTCTGTTGATCCCGGTCGGTCTGCACC
ATGCCCTGAACTCCGTGTTCTGGTTTGACGTCGCCGGCATTAACGATATTCCGAACTTCCTGGGCGGCGCTCAGTCCATT
GAGAGTGGCAAGGCGGTGGTCGGCATCACCGGTCGCTATCAGGCCGGTTTCTTCCCGATCATGATGTTCGGTCTGCCGGG
TGCGGCGCTGGCCATCTATCACTGCGCCCGTCCGGAAAATAGGGCTAAGGTCGCCGGTATCATGCTGGCAGCGGCATTTG
CCTCCTTCTTTACCGGGATCACCGAGCCGCTGGAGTTCTCCTTCATGTTCGTGGCGCCGGTGCTGTATGTGCTGCATGCC
ATTCTGACCGGTATCTCTGTATTTATTGCGGCCAGCATGCACTGGATCTCCGGCTTCGGCTTCAGCGCCGGTCTGGTGGA
TATGGTGTTGCAGTCACGTAACCCGCTGGCGACACACTGGTACATGCTGATCCCGCAGGGGCTGGTGTTCTTTGTTATCT
ACTACGTGGTGTTCCGCTTCACTATCACCAAATTCAACCTGATGACGCCGGGGCGTGAGTTGAGCGGCGCGGGTGAAGAG
GCTGATGGCAACGATGAGGATCTGGATCTGACCAAAGAAGCGGATGTCAGCGAGCTGGCCCGTCAGTATATCGCCTGCGT
CGGTGGCTCCGCCAACCTGACCGGTATCGATGCCTGTATCACCCGACTGCGTCTGACGGTGAAGGACTCTTCGCTGGTCA
ATGAAGCGATGGCTAAGAGGCTGGGCGCTTCCGGCGTAATCCGCCTGAACAAGACCAGCGTCCAGATCATCGTTGGCTTC
GTGGCCGAGAAGATCGCCAATGCAATGCGTACGGCGGGCACGGTACCTGCTGCGGCGGCGAGCTCCGCTGCTCCGGCGGC
AAAGGCCCAGGCGACGCCAAACGCCGCCGCGGGTGTCCACTCGACGCTGGTATCACCGATCACCGGTGAAGTGGTGGCCC
TGAGCGAGGTGCCGGACGAGGCGTTTGCCAGTAAAGCCGTCGGCGACGGTCTGGCGGTGCGTCCGACGGATAAGATGGTT
GTCGCACCGGCTAACGGCACCGTAGTGAAGATTTTCAACACCAATCACGCCTTCTGTCTGGAGAGTGATGACGGTGTGGA
AGTGGTGGTGCACATGGGGATCGATACCGTGGCGCTGAACGGTCAGGGCTTTAAGCGCCTGGTGGAAGAGGGGGATGTGG
TGAAGGTCGGCCAGCCGGTGCTGGAGCTGGATCTGGATTACCTGAACGCCCATGCGCGCTCCATGATAAGTCCGGTGGTA
GTCAGCAATATCGATGACTTTGGCGGCGTGGGGGCGTTGGCTTCCGGTCAGGTGGTCGCCGGTCAGAGCAAGCTGTATAC
CATTCTGAACAAGTAG

Upstream 100 bases:

>100_bases
CTCCAGTGTGGTTCATATTATCAACAAAATACTTAAATCAGTGATGATGAGACCGTTAGTGCAGTGTGTATTTGCACTGT
AGGTCCAAATAGGGGGAAAT

Downstream 100 bases:

>100_bases
CGCCGCCGTTACACGACTGCGTCACGCGGCGGGGGGCCTGAAAGGGCTCCCCGTTTTTTATTGTCCGGATAAGGGGGAGG
GGACGCGGCGCGGTTATCCG

Product: pts system, N-acetylglucosamine-specific iibc component

Products: NA

Alternate protein names: EIICBA-Nag; EII-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component; N-acetylglucosamine-specific phosphotransferase enzyme IIA component; PTS system N-acetylglucosamine-specific EIIA component [H]

Number of amino acids: Translated: 671; Mature: 670

Protein sequence:

>671_residues
MSILGYLQKVGRALMVPVATLPAAAILMGVGYWIDPVGWGGDNALAAFFIKSGSAIIDNMSVLFAIGVAYGMSKDKDGAA
ALAGFVGFLVVTTLCSPAAVAMIQKIPVDQVPAAFGKINNQFIGILVGIISAELYNRYSSVELPKALSFFSGRRLVPILT
SFLMIILAFILMYIWPVIFNGLVNFGEHIQKLGSVGAGVYAFFNRLLIPVGLHHALNSVFWFDVAGINDIPNFLGGAQSI
ESGKAVVGITGRYQAGFFPIMMFGLPGAALAIYHCARPENRAKVAGIMLAAAFASFFTGITEPLEFSFMFVAPVLYVLHA
ILTGISVFIAASMHWISGFGFSAGLVDMVLQSRNPLATHWYMLIPQGLVFFVIYYVVFRFTITKFNLMTPGRELSGAGEE
ADGNDEDLDLTKEADVSELARQYIACVGGSANLTGIDACITRLRLTVKDSSLVNEAMAKRLGASGVIRLNKTSVQIIVGF
VAEKIANAMRTAGTVPAAAASSAAPAAKAQATPNAAAGVHSTLVSPITGEVVALSEVPDEAFASKAVGDGLAVRPTDKMV
VAPANGTVVKIFNTNHAFCLESDDGVEVVVHMGIDTVALNGQGFKRLVEEGDVVKVGQPVLELDLDYLNAHARSMISPVV
VSNIDDFGGVGALASGQVVAGQSKLYTILNK

Sequences:

>Translated_671_residues
MSILGYLQKVGRALMVPVATLPAAAILMGVGYWIDPVGWGGDNALAAFFIKSGSAIIDNMSVLFAIGVAYGMSKDKDGAA
ALAGFVGFLVVTTLCSPAAVAMIQKIPVDQVPAAFGKINNQFIGILVGIISAELYNRYSSVELPKALSFFSGRRLVPILT
SFLMIILAFILMYIWPVIFNGLVNFGEHIQKLGSVGAGVYAFFNRLLIPVGLHHALNSVFWFDVAGINDIPNFLGGAQSI
ESGKAVVGITGRYQAGFFPIMMFGLPGAALAIYHCARPENRAKVAGIMLAAAFASFFTGITEPLEFSFMFVAPVLYVLHA
ILTGISVFIAASMHWISGFGFSAGLVDMVLQSRNPLATHWYMLIPQGLVFFVIYYVVFRFTITKFNLMTPGRELSGAGEE
ADGNDEDLDLTKEADVSELARQYIACVGGSANLTGIDACITRLRLTVKDSSLVNEAMAKRLGASGVIRLNKTSVQIIVGF
VAEKIANAMRTAGTVPAAAASSAAPAAKAQATPNAAAGVHSTLVSPITGEVVALSEVPDEAFASKAVGDGLAVRPTDKMV
VAPANGTVVKIFNTNHAFCLESDDGVEVVVHMGIDTVALNGQGFKRLVEEGDVVKVGQPVLELDLDYLNAHARSMISPVV
VSNIDDFGGVGALASGQVVAGQSKLYTILNK
>Mature_670_residues
SILGYLQKVGRALMVPVATLPAAAILMGVGYWIDPVGWGGDNALAAFFIKSGSAIIDNMSVLFAIGVAYGMSKDKDGAAA
LAGFVGFLVVTTLCSPAAVAMIQKIPVDQVPAAFGKINNQFIGILVGIISAELYNRYSSVELPKALSFFSGRRLVPILTS
FLMIILAFILMYIWPVIFNGLVNFGEHIQKLGSVGAGVYAFFNRLLIPVGLHHALNSVFWFDVAGINDIPNFLGGAQSIE
SGKAVVGITGRYQAGFFPIMMFGLPGAALAIYHCARPENRAKVAGIMLAAAFASFFTGITEPLEFSFMFVAPVLYVLHAI
LTGISVFIAASMHWISGFGFSAGLVDMVLQSRNPLATHWYMLIPQGLVFFVIYYVVFRFTITKFNLMTPGRELSGAGEEA
DGNDEDLDLTKEADVSELARQYIACVGGSANLTGIDACITRLRLTVKDSSLVNEAMAKRLGASGVIRLNKTSVQIIVGFV
AEKIANAMRTAGTVPAAAASSAAPAAKAQATPNAAAGVHSTLVSPITGEVVALSEVPDEAFASKAVGDGLAVRPTDKMVV
APANGTVVKIFNTNHAFCLESDDGVEVVVHMGIDTVALNGQGFKRLVEEGDVVKVGQPVLELDLDYLNAHARSMISPVVV
SNIDDFGGVGALASGQVVAGQSKLYTILNK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=679, Percent_Identity=52.1354933726068, Blast_Score=595, Evalue=1e-171,
Organism=Escherichia coli, GI1787343, Length=498, Percent_Identity=37.5502008032129, Blast_Score=302, Evalue=5e-83,
Organism=Escherichia coli, GI1787908, Length=526, Percent_Identity=34.0304182509506, Blast_Score=275, Evalue=8e-75,
Organism=Escherichia coli, GI1788757, Length=146, Percent_Identity=51.3698630136986, Blast_Score=155, Evalue=1e-38,
Organism=Escherichia coli, GI1790159, Length=436, Percent_Identity=28.8990825688073, Blast_Score=116, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 70835; Mature: 70704

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00371 PTS_EIIA_TYPE_1_HIS ; PS51093 PTS_EIIA_TYPE_1 ; PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILGYLQKVGRALMVPVATLPAAAILMGVGYWIDPVGWGGDNALAAFFIKSGSAIIDNM
CCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCHHHHHH
SVLFAIGVAYGMSKDKDGAAALAGFVGFLVVTTLCSPAAVAMIQKIPVDQVPAAFGKINN
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHCC
QFIGILVGIISAELYNRYSSVELPKALSFFSGRRLVPILTSFLMIILAFILMYIWPVIFN
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GLVNFGEHIQKLGSVGAGVYAFFNRLLIPVGLHHALNSVFWFDVAGINDIPNFLGGAQSI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHCCHHHH
ESGKAVVGITGRYQAGFFPIMMFGLPGAALAIYHCARPENRAKVAGIMLAAAFASFFTGI
CCCCEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
TEPLEFSFMFVAPVLYVLHAILTGISVFIAASMHWISGFGFSAGLVDMVLQSRNPLATHW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHH
YMLIPQGLVFFVIYYVVFRFTITKFNLMTPGRELSGAGEEADGNDEDLDLTKEADVSELA
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
RQYIACVGGSANLTGIDACITRLRLTVKDSSLVNEAMAKRLGASGVIRLNKTSVQIIVGF
HHHHHHCCCCCCCCHHHHHHHHHHEEECCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHH
VAEKIANAMRTAGTVPAAAASSAAPAAKAQATPNAAAGVHSTLVSPITGEVVALSEVPDE
HHHHHHHHHHHCCCCCCHHCCCCCCCHHCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCH
AFASKAVGDGLAVRPTDKMVVAPANGTVVKIFNTNHAFCLESDDGVEVVVHMGIDTVALN
HHHHHHCCCCEEECCCCCEEEECCCCCEEEEEECCCEEEEECCCCCEEEEEECCCEEEEC
GQGFKRLVEEGDVVKVGQPVLELDLDYLNAHARSMISPVVVSNIDDFGGVGALASGQVVA
CHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCCCEEE
GQSKLYTILNK
CCHHHHHCCCC
>Mature Secondary Structure 
SILGYLQKVGRALMVPVATLPAAAILMGVGYWIDPVGWGGDNALAAFFIKSGSAIIDNM
CHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCHHHHHH
SVLFAIGVAYGMSKDKDGAAALAGFVGFLVVTTLCSPAAVAMIQKIPVDQVPAAFGKINN
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHCC
QFIGILVGIISAELYNRYSSVELPKALSFFSGRRLVPILTSFLMIILAFILMYIWPVIFN
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GLVNFGEHIQKLGSVGAGVYAFFNRLLIPVGLHHALNSVFWFDVAGINDIPNFLGGAQSI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHCCHHHH
ESGKAVVGITGRYQAGFFPIMMFGLPGAALAIYHCARPENRAKVAGIMLAAAFASFFTGI
CCCCEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
TEPLEFSFMFVAPVLYVLHAILTGISVFIAASMHWISGFGFSAGLVDMVLQSRNPLATHW
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHH
YMLIPQGLVFFVIYYVVFRFTITKFNLMTPGRELSGAGEEADGNDEDLDLTKEADVSELA
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
RQYIACVGGSANLTGIDACITRLRLTVKDSSLVNEAMAKRLGASGVIRLNKTSVQIIVGF
HHHHHHCCCCCCCCHHHHHHHHHHEEECCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHH
VAEKIANAMRTAGTVPAAAASSAAPAAKAQATPNAAAGVHSTLVSPITGEVVALSEVPDE
HHHHHHHHHHHCCCCCCHHCCCCCCCHHCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCH
AFASKAVGDGLAVRPTDKMVVAPANGTVVKIFNTNHAFCLESDDGVEVVVHMGIDTVALN
HHHHHHCCCCEEECCCCCEEEECCCCCEEEEEECCCEEEEECCCCCEEEEEECCCEEEEC
GQGFKRLVEEGDVVKVGQPVLELDLDYLNAHARSMISPVVVSNIDDFGGVGALASGQVVA
CHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCCCEEE
GQSKLYTILNK
CCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3284790; 3056518; 8905232; 9278503 [H]