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The map label for this gene is sucB [H]

Identifier: 238920748

GI number: 238920748

Start: 2755338

End: 2756549

Strand: Reverse

Name: sucB [H]

Synonym: NT01EI_2867

Alternate gene names: 238920748

Gene position: 2756549-2755338 (Counterclockwise)

Preceding gene: 238920749

Following gene: 238920747

Centisome position: 72.31

GC content: 60.89

Gene sequence:

>1212_bases
ATGAGCAGCGTAGAAATTCTGGTTCCCGATCTGCCTGAATCCGTCGCCGACGCCACGGTAGCCACTTGGCATAAGCAGGT
GGGCGATAGCATCGGCCGCGATGAGGTCATCGTCGAGATAGAGACGGACAAAGTGGTCCTGGAAGTGCCGGCGGCGGAGG
CCGGCGTGTTGGAGGCTATCCTGGAGCCGGAGGGGACGACGGTAACGGCGCGCCAGCTGTTGGGACGGCTGCGTCCGGCC
GACGTGAGCGGCGTCGCGATCGCCGGTGGGGCGCAGACGGCAGCATCGACACCGGCTGAGCGCCATACCGCCGCTCTGGA
TACGGGCAGCAGCGATGCCCTGAGCCCCGCGGTGCGTCGTCTGGTCGCCGAACATGGCGTAGATCCGGCATCGCTGCAGG
GCAGCGGTGTCGGCGGGCGTCTGACGCGGGAGGATGTCACGAAGCATTTGGCCGGGCAGCAGAGCACAGCGCCGGTTGCT
GCGTCACCTCAGGCCGCTGCGCCGCTGAGCGCGGGGCGTGAGAAGCGGGTACCCATGACCCGCTTGCGGAAACGGGTGGC
CGAGCGCCTGCTGGAGGCGAAAAACAGCACTGCGATGCTGACCACCTTCAATGAAGTGAACATGAAGCCGATCATGGATC
TGCGCAGCCAGTACGGTGAGGTGTTTGAAAAACGTCACGGCGTGCGCCTGGGCTTTATGTCCTTTTATATCAAGGCAGTA
TTGGAGGCGTTAAAACGTTATCCGGAGGTTAACGCCGCTCTTGACGGTGAAGAGGTGGTCTACCACAACTACTTTGATAT
CAGTATTGCGGTGTCGACGCCGCGCGGACTGGTTACGCCGGTGATCCGCGATGTCGATACCCTGAGCATGGCGGATATTG
AAAAACAGATTAAAGCGCTGGCGCTGAAGGGGCGTGACGGAAAACTGACGGTGGAGGAGCTGACCGGCGGTAACTTTACC
ATTACCAACGGCGGCGTATTCGGCTCTCTGATGTCCACGCCGATTATCAATCCGCCGCAGAGCGCTATCCTGGGAATGCA
TGCCATTAAGGATCGCCCGATGGCGGTAGACGGCCAGGTGGTGATCCTGCCGATGATGTACCTGGCGCTCTCGTATGACC
ATCGCCAAATTGACGGGCGAGAGTCCGTGGGTTTCCTGGTGACGGTAAAAGAGATGTTGGAAGATCCGGCGCGTCTGCTG
CTGGATGTGTAA

Upstream 100 bases:

>100_bases
GAGCAGCAGCAGGCGTTGGTGGATGATGCCCTGAATGTGGAATAAAGGATGCGGGCGTTACCGGCCGTATTCACGACACG
CATTAATATAAGGATAAATA

Downstream 100 bases:

>100_bases
CTCCGTTTGATCCGTGCTATGCCCAGGCCCGTCACGTCGGCGGGCCTGCTACTAACAATAAGAGTCAGCCCCTATAGGGA
TAGAACATCATGAACTTACA

Product: dihydrolipoyllysine-residue succinyltransferase, E2 component of oxoglutarate dehydrogenase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSSVEILVPDLPESVADATVATWHKQVGDSIGRDEVIVEIETDKVVLEVPAAEAGVLEAILEPEGTTVTARQLLGRLRPA
DVSGVAIAGGAQTAASTPAERHTAALDTGSSDALSPAVRRLVAEHGVDPASLQGSGVGGRLTREDVTKHLAGQQSTAPVA
ASPQAAAPLSAGREKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRSQYGEVFEKRHGVRLGFMSFYIKAV
LEALKRYPEVNAALDGEEVVYHNYFDISIAVSTPRGLVTPVIRDVDTLSMADIEKQIKALALKGRDGKLTVEELTGGNFT
ITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAVDGQVVILPMMYLALSYDHRQIDGRESVGFLVTVKEMLEDPARLL
LDV

Sequences:

>Translated_403_residues
MSSVEILVPDLPESVADATVATWHKQVGDSIGRDEVIVEIETDKVVLEVPAAEAGVLEAILEPEGTTVTARQLLGRLRPA
DVSGVAIAGGAQTAASTPAERHTAALDTGSSDALSPAVRRLVAEHGVDPASLQGSGVGGRLTREDVTKHLAGQQSTAPVA
ASPQAAAPLSAGREKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRSQYGEVFEKRHGVRLGFMSFYIKAV
LEALKRYPEVNAALDGEEVVYHNYFDISIAVSTPRGLVTPVIRDVDTLSMADIEKQIKALALKGRDGKLTVEELTGGNFT
ITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAVDGQVVILPMMYLALSYDHRQIDGRESVGFLVTVKEMLEDPARLL
LDV
>Mature_402_residues
SSVEILVPDLPESVADATVATWHKQVGDSIGRDEVIVEIETDKVVLEVPAAEAGVLEAILEPEGTTVTARQLLGRLRPAD
VSGVAIAGGAQTAASTPAERHTAALDTGSSDALSPAVRRLVAEHGVDPASLQGSGVGGRLTREDVTKHLAGQQSTAPVAA
SPQAAAPLSAGREKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRSQYGEVFEKRHGVRLGFMSFYIKAVL
EALKRYPEVNAALDGEEVVYHNYFDISIAVSTPRGLVTPVIRDVDTLSMADIEKQIKALALKGRDGKLTVEELTGGNFTI
TNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAVDGQVVILPMMYLALSYDHRQIDGRESVGFLVTVKEMLEDPARLLL
DV

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=255, Percent_Identity=54.9019607843137, Blast_Score=286, Evalue=3e-77,
Organism=Homo sapiens, GI31711992, Length=432, Percent_Identity=29.6296296296296, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI110671329, Length=426, Percent_Identity=27.2300469483568, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI203098753, Length=455, Percent_Identity=27.4725274725275, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI203098816, Length=455, Percent_Identity=27.4725274725275, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI260898739, Length=168, Percent_Identity=36.3095238095238, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI1786946, Length=405, Percent_Identity=76.5432098765432, Blast_Score=635, Evalue=0.0,
Organism=Escherichia coli, GI1786305, Length=425, Percent_Identity=30.5882352941176, Blast_Score=158, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI25146366, Length=401, Percent_Identity=42.14463840399, Blast_Score=306, Evalue=1e-83,
Organism=Caenorhabditis elegans, GI17560088, Length=437, Percent_Identity=32.0366132723112, Blast_Score=171, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI17537937, Length=421, Percent_Identity=26.1282660332542, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17538894, Length=329, Percent_Identity=31.0030395136778, Blast_Score=133, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6320352, Length=412, Percent_Identity=42.4757281553398, Blast_Score=324, Evalue=1e-89,
Organism=Saccharomyces cerevisiae, GI6324258, Length=454, Percent_Identity=27.5330396475771, Blast_Score=149, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24645909, Length=224, Percent_Identity=58.4821428571429, Blast_Score=278, Evalue=4e-75,
Organism=Drosophila melanogaster, GI18859875, Length=429, Percent_Identity=28.9044289044289, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI20129315, Length=229, Percent_Identity=33.1877729257642, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24582497, Length=229, Percent_Identity=33.1877729257642, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 43200; Mature: 43069

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSVEILVPDLPESVADATVATWHKQVGDSIGRDEVIVEIETDKVVLEVPAAEAGVLEAI
CCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEEECCCCHHHHHHHH
LEPEGTTVTARQLLGRLRPADVSGVAIAGGAQTAASTPAERHTAALDTGSSDALSPAVRR
HCCCCCEEHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHH
LVAEHGVDPASLQGSGVGGRLTREDVTKHLAGQQSTAPVAASPQAAAPLSAGREKRVPMT
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
RLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRSQYGEVFEKRHGVRLGFMSFYIKAV
HHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHH
LEALKRYPEVNAALDGEEVVYHNYFDISIAVSTPRGLVTPVIRDVDTLSMADIEKQIKAL
HHHHHHCCCCCCCCCCCEEEEEEEEEEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHH
ALKGRDGKLTVEELTGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAVDGQV
HCCCCCCCEEEEEECCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEECCCC
VILPMMYLALSYDHRQIDGRESVGFLVTVKEMLEDPARLLLDV
HHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHCCHHHHHCCC
>Mature Secondary Structure 
SSVEILVPDLPESVADATVATWHKQVGDSIGRDEVIVEIETDKVVLEVPAAEAGVLEAI
CCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEEECCCCHHHHHHHH
LEPEGTTVTARQLLGRLRPADVSGVAIAGGAQTAASTPAERHTAALDTGSSDALSPAVRR
HCCCCCEEHHHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHH
LVAEHGVDPASLQGSGVGGRLTREDVTKHLAGQQSTAPVAASPQAAAPLSAGREKRVPMT
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
RLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRSQYGEVFEKRHGVRLGFMSFYIKAV
HHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHH
LEALKRYPEVNAALDGEEVVYHNYFDISIAVSTPRGLVTPVIRDVDTLSMADIEKQIKAL
HHHHHHCCCCCCCCCCCEEEEEEEEEEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHH
ALKGRDGKLTVEELTGGNFTITNGGVFGSLMSTPIINPPQSAILGMHAIKDRPMAVDGQV
HCCCCCCCEEEEEECCCCEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEECCCC
VILPMMYLALSYDHRQIDGRESVGFLVTVKEMLEDPARLLLDV
HHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]