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The map label for this gene is tolA [H]

Identifier: 238920739

GI number: 238920739

Start: 2746376

End: 2747545

Strand: Reverse

Name: tolA [H]

Synonym: NT01EI_2858

Alternate gene names: 238920739

Gene position: 2747545-2746376 (Counterclockwise)

Preceding gene: 238920740

Following gene: 238920738

Centisome position: 72.07

GC content: 59.49

Gene sequence:

>1170_bases
GTGGGAAATGCAACTGAGCAAAAAGATAAGCTGAATCGCTCTGTCATCATCTCGGTGATTTTGCACATTGTCCTGATCGG
CCTGCTGATTTGGGGCTCCCTGATGCAGGATAACCCGATGAGTGAGGGAGGGGATGGCGGCGGCGGCGGTTCTGCGATCG
ACGCGGTGATGGTGGATCCGAATGCGGTCGTGCAGCAGTACAACCGCCAGCAGGAGCAGCAGAATGCAGCCCAGCGTGCG
GAGCAGCAGCGTCAGCAGAAAGCGGAAAAGCAGGCGCAGGAGATGCAGCGTAAGCGGGCTGAAGAGCAACAGCGCCTGAA
GGTATTGGAGAAAGAGCGCTTGCAGGCGCAGGAAGATGCCAAGCAGGCGGCGGAAGAGGCCAAACAAGCCGCGGAGCAGG
CGAAAAAACAGGCAGCGGAAGAGGCGAAGAAAGCGGCTGCGCTGGCGGTGCAGCAGGCGAAGGAGGAGGCGGCGAAAGCG
GCTGCTGAAGCTAAGAAACAGGCCGAGGAAGAGGTGAAAAAAGCCGCCCTCGCCGCGAAGAAACAGGCTGACGAGGCGGC
GAAGAAGGCCGCCGACGCCGCGAAGAAGCAGGCGGCGGAAGAGGCGAAGAAAGCGGCGGAGGCCAAGGCTGCGGCGGACG
CGAAGGCGGAGGCCAAGGCGCAGGTGGCGGCAGAAGCGAAGGCAAAGGCTGATGCCAAAAAAGCTGCTGAGGCCAAAAAA
GCCGCTGAGGCGAAGAAGGCGGCGGCGGCCGCGAAACAGGCTGATGAGGTGGACGATCTGTTTGGCGGTCTGGCATCGTC
GAAAAATGCACCGAAAGGTACTGCCAATGGTGGCACGCCCGGTAACAGCGGTAAGGCGGGCCCGGATGGTAAAGGCAATA
GTAAGGCTTCTGGCGCCTCTGGTGCAGAAATTAGCGGCTATGGAAATCAAATTAGAAGCGCAATTATGGCGAAGTTTTAT
ACTGACCCAAGTTATGCTGGAAAAACCTGCGATCTACGCATCAAGCTGGCGCCCGATGGCCTGCTGATCGATGTAAAGGC
GGAAGGAGGCGATCCGGCGTTGTGTCAGGCTGCACTTTCCGCTGCGCGTCAGGCGCGCTATCCCAAGCCGCCCAGCGACG
CGGTGTATCAGGTATTTAAAAATGCACCGGTGGTGTTTCAGCCGCAGTGA

Upstream 100 bases:

>100_bases
GCCTGTACAGGCTGCGTGTCTTTATTTTACGGTGCCAGCGCGGCGAGTGGTGGCCTGGCCCGGCAGGATCTTGGAAGCGA
CTAGCGGTTGGAATCGAATT

Downstream 100 bases:

>100_bases
TGAACTCGGCGCCACGGCGGCAATCGGCGGATCGTTATCGGATTAGGCTGATGGTCTTCTGCGGCGGCATGTAGTGTTGT
TGACATTGATTTGTTTAAAT

Product: protein TolA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MGNATEQKDKLNRSVIISVILHIVLIGLLIWGSLMQDNPMSEGGDGGGGGSAIDAVMVDPNAVVQQYNRQQEQQNAAQRA
EQQRQQKAEKQAQEMQRKRAEEQQRLKVLEKERLQAQEDAKQAAEEAKQAAEQAKKQAAEEAKKAAALAVQQAKEEAAKA
AAEAKKQAEEEVKKAALAAKKQADEAAKKAADAAKKQAAEEAKKAAEAKAAADAKAEAKAQVAAEAKAKADAKKAAEAKK
AAEAKKAAAAAKQADEVDDLFGGLASSKNAPKGTANGGTPGNSGKAGPDGKGNSKASGASGAEISGYGNQIRSAIMAKFY
TDPSYAGKTCDLRIKLAPDGLLIDVKAEGGDPALCQAALSAARQARYPKPPSDAVYQVFKNAPVVFQPQ

Sequences:

>Translated_389_residues
MGNATEQKDKLNRSVIISVILHIVLIGLLIWGSLMQDNPMSEGGDGGGGGSAIDAVMVDPNAVVQQYNRQQEQQNAAQRA
EQQRQQKAEKQAQEMQRKRAEEQQRLKVLEKERLQAQEDAKQAAEEAKQAAEQAKKQAAEEAKKAAALAVQQAKEEAAKA
AAEAKKQAEEEVKKAALAAKKQADEAAKKAADAAKKQAAEEAKKAAEAKAAADAKAEAKAQVAAEAKAKADAKKAAEAKK
AAEAKKAAAAAKQADEVDDLFGGLASSKNAPKGTANGGTPGNSGKAGPDGKGNSKASGASGAEISGYGNQIRSAIMAKFY
TDPSYAGKTCDLRIKLAPDGLLIDVKAEGGDPALCQAALSAARQARYPKPPSDAVYQVFKNAPVVFQPQ
>Mature_388_residues
GNATEQKDKLNRSVIISVILHIVLIGLLIWGSLMQDNPMSEGGDGGGGGSAIDAVMVDPNAVVQQYNRQQEQQNAAQRAE
QQRQQKAEKQAQEMQRKRAEEQQRLKVLEKERLQAQEDAKQAAEEAKQAAEQAKKQAAEEAKKAAALAVQQAKEEAAKAA
AEAKKQAEEEVKKAALAAKKQADEAAKKAADAAKKQAAEEAKKAAEAKAAADAKAEAKAQVAAEAKAKADAKKAAEAKKA
AEAKKAAAAAKQADEVDDLFGGLASSKNAPKGTANGGTPGNSGKAGPDGKGNSKASGASGAEISGYGNQIRSAIMAKFYT
DPSYAGKTCDLRIKLAPDGLLIDVKAEGGDPALCQAALSAARQARYPKPPSDAVYQVFKNAPVVFQPQ

Specific function: Involved in the tonB-independent uptake of group A colicins (colicins A, E1, E2, E3, and K). Necessary for the colicins to reach their respective targets after initial binding to the bacteria. Also involved in the translocation of bacteriophage DNA [H]

COG id: COG3064

COG function: function code M; Membrane protein involved in colicin uptake

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786960, Length=129, Percent_Identity=65.1162790697674, Blast_Score=165, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014161 [H]

Pfam domain/function: PF06519 TolA [H]

EC number: NA

Molecular weight: Translated: 40563; Mature: 40432

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHCCCCCCCHHHHHHHHHCCCCEECCC
>Mature Secondary Structure 
GNATEQKDKLNRSVIISVILHIVLIGLLIWGSLMQDNPMSEGGDGGGGGSAIDAVMVDP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHEEEECC
NAVVQQYNRQQEQQNAAQRAEQQRQQKAEKQAQEMQRKRAEEQQRLKVLEKERLQAQEDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQAAEEAKQAAEQAKKQAAEEAKKAAALAVQQAKEEAAKAAAEAKKQAEEEVKKAALAAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQADEAAKKAADAAKKQAAEEAKKAAEAKAAADAKAEAKAQVAAEAKAKADAKKAAEAKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAEAKKAAAAAKQADEVDDLFGGLASSKNAPKGTANGGTPGNSGKAGPDGKGNSKASGAS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GAEISGYGNQIRSAIMAKFYTDPSYAGKTCDLRIKLAPDGLLIDVKAEGGDPALCQAALS
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCEEEEEECCCCCHHHHHHHHH
AARQARYPKPPSDAVYQVFKNAPVVFQPQ
HHHHCCCCCCCHHHHHHHHHCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2687247; 8905232; 9278503; 2068069; 8978668; 10404600 [H]