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The map label for this gene is tolB

Identifier: 238920738

GI number: 238920738

Start: 2744939

End: 2746231

Strand: Reverse

Name: tolB

Synonym: NT01EI_2857

Alternate gene names: 238920738

Gene position: 2746231-2744939 (Counterclockwise)

Preceding gene: 238920739

Following gene: 238920737

Centisome position: 72.04

GC content: 59.55

Gene sequence:

>1293_bases
ATGAAGCAGGCGTTTCGTTTGATGGTGGGGCTACTCGTCCTGTGGGCCAGCGTGCTCCATGCCGAAGTCCGAATCGTGAT
AACCCAGGGGGTAGACTCAGCCCGACCGATTGGTGTAGTGCCGTTTAAGTGGATGGGGCCGGGTACGGCACCGGAAAATA
TCGGTGGCATCATCGCCGCTGATCTGCGCAACAGTGGTAAGTTTAATCCGATCGATGCCAGCCGGATGCCGCAGCAACCG
TCGGCGGCCGCCGAGGTCTCTCCTGCCGCCTGGACGGCGCTGGGGATCGATGCTGTGGTGGTCGGTCAGGTGCAGCCAGG
CGCGGACGGCAGCTATCTGGTCTCTTACCAGCTGGTGGATACCTCCGGAAACCCGGGCAGTGTGCTGGTGCAGAACCAGT
ATAAGGTGACCGGGCAGTGGCTGCGCTACGCAGCCCATACTGCCAGCGATGAAGTGTTTGAGAAGCTGACCGGTATCAAG
GGGGCTTTCCGCACCCGTATTGCCTATGTGGTGCAGACCAACGGTGGCCAGTTCCCCTATGAGCTGCGTGTGGCTGACTA
TGATGGCTATAATCAGTTTGTGGTACATCGCTCCGCCGAGCCGTTGATGTCGCCGGCCTGGTCACCGGACGGCAGCAAGC
TGGCCTATGTGACCTTTGAGAGCGGTCGCTCGGCGCTGGTGATCCAGACCCTGTCCAACGGCGCGATCCGTCAGGTGGCT
TCCTTCCCGCGTCATAACGGTTCTCCGGCCTTCTCTCCGGATGGCAGCAAGCTGGCGTTTGCGCTCTCCAAGACCGGTAG
TCTCAACCTGTACGTGATGGATCTGGCCTCCGGCGCCATCCGCCAGGTGACCGATGGACGCAGCAACAACACGGAGCCCA
CCTGGTTCCCGGACAGCCAAAACCTGGCCTTTACCTCTGACCAGGCTGGCCGGCCGCAGATTTATAAAATGAACATCAAC
GGCGGTGTCGCGCAGCGCCTGACCTGGGAAGGTTCCCAGAATCAGGATGCCGAAGTGAGCCCTGACGGGAAATTCCTGGT
CATGGTGAGCAGCAGTGCAGGCGCGCAGCATATTGCTAAACTGGATCTTGGAACGAATGCCACTCAGGTTCTTACGGATA
CGTTCCTGGATGAAACGCCGAGTATCGCGCCGAACGGCACCATGGTGATTTACAGTTCTACCCAGGGACTGGGCTCCGTG
CTGCAGTTAGTATCGATAGACGGGCGTTTTAAAGCGCGTCTTCCGGCGACCGATGGTCAGGTTAAGTTCCCTGCCTGGTC
GCCGTATCTGTGA

Upstream 100 bases:

>100_bases
AGGCTGATGGTCTTCTGCGGCGGCATGTAGTGTTGTTGACATTGATTTGTTTAAATTCTGCTAATTTATCGTGGCCAGAG
GGTCGGATAAGGGAGATAAG

Downstream 100 bases:

>100_bases
TGCATGAATAAAACTATACTTATATAAATTTAAAAGGATCAATAAGATGCAACTGAACAAAGTTCTCAAGGGGTTGATGC
TGGTACTGCCGATCATGGCC

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MKQAFRLMVGLLVLWASVLHAEVRIVITQGVDSARPIGVVPFKWMGPGTAPENIGGIIAADLRNSGKFNPIDASRMPQQP
SAAAEVSPAAWTALGIDAVVVGQVQPGADGSYLVSYQLVDTSGNPGSVLVQNQYKVTGQWLRYAAHTASDEVFEKLTGIK
GAFRTRIAYVVQTNGGQFPYELRVADYDGYNQFVVHRSAEPLMSPAWSPDGSKLAYVTFESGRSALVIQTLSNGAIRQVA
SFPRHNGSPAFSPDGSKLAFALSKTGSLNLYVMDLASGAIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQIYKMNIN
GGVAQRLTWEGSQNQDAEVSPDGKFLVMVSSSAGAQHIAKLDLGTNATQVLTDTFLDETPSIAPNGTMVIYSSTQGLGSV
LQLVSIDGRFKARLPATDGQVKFPAWSPYL

Sequences:

>Translated_430_residues
MKQAFRLMVGLLVLWASVLHAEVRIVITQGVDSARPIGVVPFKWMGPGTAPENIGGIIAADLRNSGKFNPIDASRMPQQP
SAAAEVSPAAWTALGIDAVVVGQVQPGADGSYLVSYQLVDTSGNPGSVLVQNQYKVTGQWLRYAAHTASDEVFEKLTGIK
GAFRTRIAYVVQTNGGQFPYELRVADYDGYNQFVVHRSAEPLMSPAWSPDGSKLAYVTFESGRSALVIQTLSNGAIRQVA
SFPRHNGSPAFSPDGSKLAFALSKTGSLNLYVMDLASGAIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQIYKMNIN
GGVAQRLTWEGSQNQDAEVSPDGKFLVMVSSSAGAQHIAKLDLGTNATQVLTDTFLDETPSIAPNGTMVIYSSTQGLGSV
LQLVSIDGRFKARLPATDGQVKFPAWSPYL
>Mature_430_residues
MKQAFRLMVGLLVLWASVLHAEVRIVITQGVDSARPIGVVPFKWMGPGTAPENIGGIIAADLRNSGKFNPIDASRMPQQP
SAAAEVSPAAWTALGIDAVVVGQVQPGADGSYLVSYQLVDTSGNPGSVLVQNQYKVTGQWLRYAAHTASDEVFEKLTGIK
GAFRTRIAYVVQTNGGQFPYELRVADYDGYNQFVVHRSAEPLMSPAWSPDGSKLAYVTFESGRSALVIQTLSNGAIRQVA
SFPRHNGSPAFSPDGSKLAFALSKTGSLNLYVMDLASGAIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQIYKMNIN
GGVAQRLTWEGSQNQDAEVSPDGKFLVMVSSSAGAQHIAKLDLGTNATQVLTDTFLDETPSIAPNGTMVIYSSTQGLGSV
LQLVSIDGRFKARLPATDGQVKFPAWSPYL

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=430, Percent_Identity=84.6511627906977, Blast_Score=759, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_EDWI9 (C5BEL8)

Other databases:

- EMBL:   CP001600
- RefSeq:   YP_002934253.1
- ProteinModelPortal:   C5BEL8
- GeneID:   7959499
- GenomeReviews:   CP001600_GR
- KEGG:   eic:NT01EI_2857
- OMA:   RVADYDG
- ProtClustDB:   PRK03629
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943
- Gene3D:   G3DSA:3.40.50.10070
- Gene3D:   G3DSA:2.130.10.10
- TIGRFAMs:   TIGR02800

Pfam domain/function: PF07676 PD40; PF04052 TolB_N; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 46048; Mature: 46048

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQAFRLMVGLLVLWASVLHAEVRIVITQGVDSARPIGVVPFKWMGPGTAPENIGGIIAA
CCHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCEEEE
DLRNSGKFNPIDASRMPQQPSAAAEVSPAAWTALGIDAVVVGQVQPGADGSYLVSYQLVD
ECCCCCCCCCCCHHCCCCCCCCCCCCCCCCEEEECCCEEEEEEECCCCCCCEEEEEEEEE
TSGNPGSVLVQNQYKVTGQWLRYAAHTASDEVFEKLTGIKGAFRTRIAYVVQTNGGQFPY
CCCCCCEEEEECCEEEHHHHHHHHHHCCHHHHHHHHHCCCHHHEEEEEEEEECCCCCCCE
ELRVADYDGYNQFVVHRSAEPLMSPAWSPDGSKLAYVTFESGRSALVIQTLSNGAIRQVA
EEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCHHHHHH
SFPRHNGSPAFSPDGSKLAFALSKTGSLNLYVMDLASGAIRQVTDGRSNNTEPTWFPDSQ
HCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCHHHHHHCCCCCCCCCCCCCCCC
NLAFTSDQAGRPQIYKMNINGGVAQRLTWEGSQNQDAEVSPDGKFLVMVSSSAGAQHIAK
CEEEECCCCCCCEEEEEECCCCEEEEEEECCCCCCCCEECCCCCEEEEEECCCCCCEEEE
LDLGTNATQVLTDTFLDETPSIAPNGTMVIYSSTQGLGSVLQLVSIDGRFKARLPATDGQ
EECCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEECCCCCCC
VKFPAWSPYL
EECCCCCCCC
>Mature Secondary Structure
MKQAFRLMVGLLVLWASVLHAEVRIVITQGVDSARPIGVVPFKWMGPGTAPENIGGIIAA
CCHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCEEEE
DLRNSGKFNPIDASRMPQQPSAAAEVSPAAWTALGIDAVVVGQVQPGADGSYLVSYQLVD
ECCCCCCCCCCCHHCCCCCCCCCCCCCCCCEEEECCCEEEEEEECCCCCCCEEEEEEEEE
TSGNPGSVLVQNQYKVTGQWLRYAAHTASDEVFEKLTGIKGAFRTRIAYVVQTNGGQFPY
CCCCCCEEEEECCEEEHHHHHHHHHHCCHHHHHHHHHCCCHHHEEEEEEEEECCCCCCCE
ELRVADYDGYNQFVVHRSAEPLMSPAWSPDGSKLAYVTFESGRSALVIQTLSNGAIRQVA
EEEEEECCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCHHHHHH
SFPRHNGSPAFSPDGSKLAFALSKTGSLNLYVMDLASGAIRQVTDGRSNNTEPTWFPDSQ
HCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCHHHHHHCCCCCCCCCCCCCCCC
NLAFTSDQAGRPQIYKMNINGGVAQRLTWEGSQNQDAEVSPDGKFLVMVSSSAGAQHIAK
CEEEECCCCCCCEEEEEECCCCEEEEEEECCCCCCCCEECCCCCEEEEEECCCCCCEEEE
LDLGTNATQVLTDTFLDETPSIAPNGTMVIYSSTQGLGSVLQLVSIDGRFKARLPATDGQ
EECCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEECCCCCCC
VKFPAWSPYL
EECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA