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The map label for this gene is rlmF [H]

Identifier: 238920487

GI number: 238920487

Start: 2491252

End: 2492181

Strand: Reverse

Name: rlmF [H]

Synonym: NT01EI_2598

Alternate gene names: 238920487

Gene position: 2492181-2491252 (Counterclockwise)

Preceding gene: 238920489

Following gene: 238920483

Centisome position: 65.37

GC content: 67.31

Gene sequence:

>930_bases
ATGAACCATAAGCCAGTCACGCCGTCGGATCGGCACCAGAAAGCGGGCCTGCACCCGCGCAACCGCCATCATGGCCGCTA
CGATATGGCGGTGCTGTGCCGGATCTCTCCCGCGCTGGGGGCCTTTGTCCGGGTGACGCCGGGCGGCGGCGAAAGCATCG
ACTTCGCCGATCCACAGGCGGTCCGGGCGCTCAACCGGGCGCTGCTGGCGCAGGACTATGGCATCGCGCACTGGGATATT
CCCGCACAGTTTCTCTGTCCGCCGATCCCGGGCCGGGCCGACTACCTGCATTATCTGGCCGATCTGCTGGCCAGCACCCG
TTCAGACGGCGCCATTCCGCGCGGCGCGCAGACCTGCGTGCTGGATATCGGCACCGGTGCCAACTGTATCTACCCGCTGC
TGGGGCAAGCCGAATACGGCTGGCGCTTTACCGGCAGCGATATTTCGGCGCAGGCGCTGGCGGCGGCAGCGGCCATCATC
CGGGCCAATCCGGCGCTGGGCACGCGGATTCGCCTGCGTCGTCAGCGTCAGCCGCAGGCGATTTTTCAGGGGATTATCAA
GGCCGGAGAGTACTACGCCGCGACGATGTGCAATCCGCCCTTTCACCGCAGCGCGGCGGAGGCCGCCGCGGGCAGCCAGA
GAAAGCTGACCAACCTGGGGCTGCGACCGCCGCAGGCGGCGCGACTGAACTTTGGCGGCAGGGCGGATGAGCTGTGGTGC
GACGGCGGTGAGCAGGCGTTCATTCTGCGGATGATCGCGGAGAGCCGCGCGTTTGCGACGCAGGTGGGGTGGTTTACCTC
GCTGGTGTCCCGACGGGAGACGCTGCCGGCGCTGCGCAGAGCGCTGCGCGCAATCCCGGTGCAGGAGATGCGTGAGGTGA
CGATGGCGCAGGGGCAGAAGGTGAGCCGCTTTATCGCCTGGCGATTCTGA

Upstream 100 bases:

>100_bases
CCGGCGACGGGTTGCTGAGGGAAAGCGCAGGAGGGTAGGTGTCCGGCGCGGAAATGGGTATACTTGCCGCCCTGTTTGAG
CGACTCCCCCGAAGTGAATC

Downstream 100 bases:

>100_bases
TACCGCACCGGGGCCGCCTGCGTGGGCGGTCCCGGCGGACGATCACGCGACGTGCTGCAGGAACTCGCGCAGACGCTCGT
TGGGCGGCGTATGGATCAGC

Product: SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA mA1618 methyltransferase; rRNA adenine N-6-methyltransferase [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MNHKPVTPSDRHQKAGLHPRNRHHGRYDMAVLCRISPALGAFVRVTPGGGESIDFADPQAVRALNRALLAQDYGIAHWDI
PAQFLCPPIPGRADYLHYLADLLASTRSDGAIPRGAQTCVLDIGTGANCIYPLLGQAEYGWRFTGSDISAQALAAAAAII
RANPALGTRIRLRRQRQPQAIFQGIIKAGEYYAATMCNPPFHRSAAEAAAGSQRKLTNLGLRPPQAARLNFGGRADELWC
DGGEQAFILRMIAESRAFATQVGWFTSLVSRRETLPALRRALRAIPVQEMREVTMAQGQKVSRFIAWRF

Sequences:

>Translated_309_residues
MNHKPVTPSDRHQKAGLHPRNRHHGRYDMAVLCRISPALGAFVRVTPGGGESIDFADPQAVRALNRALLAQDYGIAHWDI
PAQFLCPPIPGRADYLHYLADLLASTRSDGAIPRGAQTCVLDIGTGANCIYPLLGQAEYGWRFTGSDISAQALAAAAAII
RANPALGTRIRLRRQRQPQAIFQGIIKAGEYYAATMCNPPFHRSAAEAAAGSQRKLTNLGLRPPQAARLNFGGRADELWC
DGGEQAFILRMIAESRAFATQVGWFTSLVSRRETLPALRRALRAIPVQEMREVTMAQGQKVSRFIAWRF
>Mature_309_residues
MNHKPVTPSDRHQKAGLHPRNRHHGRYDMAVLCRISPALGAFVRVTPGGGESIDFADPQAVRALNRALLAQDYGIAHWDI
PAQFLCPPIPGRADYLHYLADLLASTRSDGAIPRGAQTCVLDIGTGANCIYPLLGQAEYGWRFTGSDISAQALAAAAAII
RANPALGTRIRLRRQRQPQAIFQGIIKAGEYYAATMCNPPFHRSAAEAAAGSQRKLTNLGLRPPQAARLNFGGRADELWC
DGGEQAFILRMIAESRAFATQVGWFTSLVSRRETLPALRRALRAIPVQEMREVTMAQGQKVSRFIAWRF

Specific function: Specifically methylates the adenine in position 1618 of 23S rRNA [H]

COG id: COG3129

COG function: function code R; Predicted SAM-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. METT10D/rlmF family [H]

Homologues:

Organism=Homo sapiens, GI122114654, Length=296, Percent_Identity=31.7567567567568, Blast_Score=153, Evalue=2e-37,
Organism=Escherichia coli, GI87081786, Length=297, Percent_Identity=61.952861952862, Blast_Score=376, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI32564931, Length=323, Percent_Identity=25.6965944272446, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI32564933, Length=323, Percent_Identity=23.2198142414861, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281363415, Length=299, Percent_Identity=26.4214046822742, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI19922302, Length=299, Percent_Identity=26.4214046822742, Blast_Score=101, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016909
- InterPro:   IPR010286 [H]

Pfam domain/function: PF05971 Methyltransf_10 [H]

EC number: =2.1.1.181 [H]

Molecular weight: Translated: 33905; Mature: 33905

Theoretical pI: Translated: 10.55; Mature: 10.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHKPVTPSDRHQKAGLHPRNRHHGRYDMAVLCRISPALGAFVRVTPGGGESIDFADPQA
CCCCCCCCCCCHHHCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCHHH
VRALNRALLAQDYGIAHWDIPAQFLCPPIPGRADYLHYLADLLASTRSDGAIPRGAQTCV
HHHHHHHHHHHCCCCEEECCCHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
LDIGTGANCIYPLLGQAEYGWRFTGSDISAQALAAAAAIIRANPALGTRIRLRRQRQPQA
EECCCCCCHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHH
IFQGIIKAGEYYAATMCNPPFHRSAAEAAAGSQRKLTNLGLRPPQAARLNFGGRADELWC
HHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHCCCCCCCCCEECCCCCCCCEEE
DGGEQAFILRMIAESRAFATQVGWFTSLVSRRETLPALRRALRAIPVQEMREVTMAQGQK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHH
VSRFIAWRF
HHHHHHCCC
>Mature Secondary Structure
MNHKPVTPSDRHQKAGLHPRNRHHGRYDMAVLCRISPALGAFVRVTPGGGESIDFADPQA
CCCCCCCCCCCHHHCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCHHH
VRALNRALLAQDYGIAHWDIPAQFLCPPIPGRADYLHYLADLLASTRSDGAIPRGAQTCV
HHHHHHHHHHHCCCCEEECCCHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
LDIGTGANCIYPLLGQAEYGWRFTGSDISAQALAAAAAIIRANPALGTRIRLRRQRQPQA
EECCCCCCHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHH
IFQGIIKAGEYYAATMCNPPFHRSAAEAAAGSQRKLTNLGLRPPQAARLNFGGRADELWC
HHHHHHHCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHCCCCCCCCCEECCCCCCCCEEE
DGGEQAFILRMIAESRAFATQVGWFTSLVSRRETLPALRRALRAIPVQEMREVTMAQGQK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHH
VSRFIAWRF
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA