Click here to switch to the map view.

The map label for this gene is fruB [H]

Identifier: 238920481

GI number: 238920481

Start: 2486828

End: 2487964

Strand: Reverse

Name: fruB [H]

Synonym: NT01EI_2592

Alternate gene names: 238920481

Gene position: 2487964-2486828 (Counterclockwise)

Preceding gene: 238920483

Following gene: 238920480

Centisome position: 65.26

GC content: 63.15

Gene sequence:

>1137_bases
ATGTTTCATCTGACCCTACAGGATATCCATCTGGCAGCGGGGGCTGACAGTAAAGAGCAGGCCATCAGGCAGGTGGCGGC
GGCGCTGGTCGCGGCGGGCTACGTCGGCGAGGGCTATGTACAGGGCATGCTGCAGCGCGAGAAGCAGACGTCGACCTACC
TGGGCAACGGTATCGCGATTCCCCATGGCACGACGGATACCCGGGATCAGGTGCTGAACACCGGAGTTCAGGTGTTTCAG
TTTCCGCAGGGGATTGCGTGGGGCGAGGGGCAGCGGGCCTATGTGGTGATCGGTATCGCCGCCAAGTCCGATGAGCACCT
GATGCTGCTGCGTCAGCTGACCCACGTGCTGAGCGACGACGGCGTAAGCCGGGAGCTGGCGCAGACCACGTCGGTGGAGG
CGCTGCGCAGCCTGTTGCTGGGTGAGCGCAGCGTTGCCGAATTCCGCTTTGATATCAGCATGGTGACACTGGGGGTGGAG
ACCGACAGCCTGATGACGCTGCAGGCGCTGAACGCCGGGCGCCTGCAGCAGGTGGGGGCGGTCAGCGGCGAGTTTGTCAG
CGATGTCATCCGCCGCGCGCCGCTGTGTCTGGGGCAGGGTATTTGGCTCTGCGACAGCGCGCTGGGTGTTCAGGGCAGCG
CGGCAGCGCTGGCGCGTCCCCGTCAGCCCTTTACCCTCGATGGGGAGCCGGTGGCATTGCTGCTGACCGTTGCGATGGCC
GATGCGCAGCCGGAGGGGATGCTGGACTATCTGAGCCGTCTGCTGCAGCGTCAGCTGGCGGAACGTTTGCTGACGGCGCA
GACCCCGGCAGAGATCGTTGCGTTGCTGACCAGCGAGCTGACAGAGAGCGGTGAGAGCCTGAGCGCCAGCTTTACCGTTC
GTAATGAGCATGGCCTGCACGCCCGACCGGGCGCGCAGTTGGTGAATACCATTAAGCAGTTTGCAAGCCAGATAACCGTT
GCCAACCTTGATGGCAACGGGGTTCCGGTCAATGGCCGCAGTCTGATGAAGGTTGTCGCACTGGGGGTTCAAAAAGGCCA
TCGTCTGCGCTTTACTGCCAGCGGCAGTGATGCGCAGCAGGCATTAGACGTTATTGGCCAGGCCATCGCGAGTGGCCTTG
GCGAGGAAATATCATGA

Upstream 100 bases:

>100_bases
GGGAAGCGCGGCAACCCGGGTTTTCTTTCCCAATCGAGCTGAAACGATTCAATTTCCCAGTGACGCACAACGCACATGGT
ATGACGGACGAGGAGCATCT

Downstream 100 bases:

>100_bases
GCAGAAGAGTTGCAACCATTACCTTAAACCCGGCCTATGATCTGGTTGGTTACTGTGCGGAGATTGAGCGCGGTGAGGTT
AACCGGGTACAAACCGCCGG

Product: bifunctional PTS system fructose-specific transporter subunit IIA/HPr protein

Products: protein histidine; sugar phosphate

Alternate protein names: MTP; Phosphotransferase FPr protein; Pseudo-HPr; Phosphocarrier protein HPr; Protein H; Fructose-specific phosphotransferase enzyme IIA component; PTS system fructose-specific EIIA component [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MFHLTLQDIHLAAGADSKEQAIRQVAAALVAAGYVGEGYVQGMLQREKQTSTYLGNGIAIPHGTTDTRDQVLNTGVQVFQ
FPQGIAWGEGQRAYVVIGIAAKSDEHLMLLRQLTHVLSDDGVSRELAQTTSVEALRSLLLGERSVAEFRFDISMVTLGVE
TDSLMTLQALNAGRLQQVGAVSGEFVSDVIRRAPLCLGQGIWLCDSALGVQGSAAALARPRQPFTLDGEPVALLLTVAMA
DAQPEGMLDYLSRLLQRQLAERLLTAQTPAEIVALLTSELTESGESLSASFTVRNEHGLHARPGAQLVNTIKQFASQITV
ANLDGNGVPVNGRSLMKVVALGVQKGHRLRFTASGSDAQQALDVIGQAIASGLGEEIS

Sequences:

>Translated_378_residues
MFHLTLQDIHLAAGADSKEQAIRQVAAALVAAGYVGEGYVQGMLQREKQTSTYLGNGIAIPHGTTDTRDQVLNTGVQVFQ
FPQGIAWGEGQRAYVVIGIAAKSDEHLMLLRQLTHVLSDDGVSRELAQTTSVEALRSLLLGERSVAEFRFDISMVTLGVE
TDSLMTLQALNAGRLQQVGAVSGEFVSDVIRRAPLCLGQGIWLCDSALGVQGSAAALARPRQPFTLDGEPVALLLTVAMA
DAQPEGMLDYLSRLLQRQLAERLLTAQTPAEIVALLTSELTESGESLSASFTVRNEHGLHARPGAQLVNTIKQFASQITV
ANLDGNGVPVNGRSLMKVVALGVQKGHRLRFTASGSDAQQALDVIGQAIASGLGEEIS
>Mature_378_residues
MFHLTLQDIHLAAGADSKEQAIRQVAAALVAAGYVGEGYVQGMLQREKQTSTYLGNGIAIPHGTTDTRDQVLNTGVQVFQ
FPQGIAWGEGQRAYVVIGIAAKSDEHLMLLRQLTHVLSDDGVSRELAQTTSVEALRSLLLGERSVAEFRFDISMVTLGVE
TDSLMTLQALNAGRLQQVGAVSGEFVSDVIRRAPLCLGQGIWLCDSALGVQGSAAALARPRQPFTLDGEPVALLLTVAMA
DAQPEGMLDYLSRLLQRQLAERLLTAQTPAEIVALLTSELTESGESLSASFTVRNEHGLHARPGAQLVNTIKQFASQITV
ANLDGNGVPVNGRSLMKVVALGVQKGHRLRFTASGSDAQQALDVIGQAIASGLGEEIS

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG4668

COG function: function code G; Mannitol/fructose-specific phosphotransferase system, IIA domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788494, Length=375, Percent_Identity=63.7333333333333, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI1790027, Length=145, Percent_Identity=46.8965517241379, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114 [H]

Pfam domain/function: PF00381 PTS-HPr; PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 40172; Mature: 40172

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00369 PTS_HPR_HIS ; PS00589 PTS_HPR_SER ; PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFHLTLQDIHLAAGADSKEQAIRQVAAALVAAGYVGEGYVQGMLQREKQTSTYLGNGIAI
CEEEEEEHEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEC
PHGTTDTRDQVLNTGVQVFQFPQGIAWGEGQRAYVVIGIAAKSDEHLMLLRQLTHVLSDD
CCCCCCHHHHHHHCCHHHHCCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHCCC
GVSRELAQTTSVEALRSLLLGERSVAEFRFDISMVTLGVETDSLMTLQALNAGRLQQVGA
CCHHHHHHHHHHHHHHHHHHCCHHHHHHEEEEEEEEEECCCCCHHHHHHCCCCHHHHHHC
VSGEFVSDVIRRAPLCLGQGIWLCDSALGVQGSAAALARPRQPFTLDGEPVALLLTVAMA
CCHHHHHHHHHHCCHHHCCCCCEECCCCCCCCCHHHHCCCCCCCEECCCCHHHHHHHHHH
DAQPEGMLDYLSRLLQRQLAERLLTAQTPAEIVALLTSELTESGESLSASFTVRNEHGLH
CCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
ARPGAQLVNTIKQFASQITVANLDGNGVPVNGRSLMKVVALGVQKGHRLRFTASGSDAQQ
CCCHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHH
ALDVIGQAIASGLGEEIS
HHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFHLTLQDIHLAAGADSKEQAIRQVAAALVAAGYVGEGYVQGMLQREKQTSTYLGNGIAI
CEEEEEEHEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEC
PHGTTDTRDQVLNTGVQVFQFPQGIAWGEGQRAYVVIGIAAKSDEHLMLLRQLTHVLSDD
CCCCCCHHHHHHHCCHHHHCCCCCCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHCCC
GVSRELAQTTSVEALRSLLLGERSVAEFRFDISMVTLGVETDSLMTLQALNAGRLQQVGA
CCHHHHHHHHHHHHHHHHHHCCHHHHHHEEEEEEEEEECCCCCHHHHHHCCCCHHHHHHC
VSGEFVSDVIRRAPLCLGQGIWLCDSALGVQGSAAALARPRQPFTLDGEPVALLLTVAMA
CCHHHHHHHHHHCCHHHCCCCCEECCCCCCCCCHHHHCCCCCCCEECCCCHHHHHHHHHH
DAQPEGMLDYLSRLLQRQLAERLLTAQTPAEIVALLTSELTESGESLSASFTVRNEHGLH
CCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCC
ARPGAQLVNTIKQFASQITVANLDGNGVPVNGRSLMKVVALGVQKGHRLRFTASGSDAQQ
CCCHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHH
ALDVIGQAIASGLGEEIS
HHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]