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The map label for this gene is nfsA [H]

Identifier: 238920417

GI number: 238920417

Start: 2427583

End: 2428305

Strand: Reverse

Name: nfsA [H]

Synonym: NT01EI_2527

Alternate gene names: 238920417

Gene position: 2428305-2427583 (Counterclockwise)

Preceding gene: 238920419

Following gene: 238920416

Centisome position: 63.7

GC content: 61.13

Gene sequence:

>723_bases
ATGATGTCGACCATCGATCTACTGCGTGATCACCGTTCTATCCGCCAGTTTAGCGCCGAAGAGATCGGCGAAGCACAGCG
GCTGGCTATTCTGGAGGCGGCGCGTGCAACGTCATCGTCCAGCTTTCTGCAGTGTACGTCGATTATCCGGATCACCGAGC
GCGCGCTACGTGAGCAGCTGGTCGAGCTGGCCGGTGGGCAGCAGTACGTGCTGGACGCCGCCGAGTTCTGGGTGTTCTGC
GCAGACTTTCAGCGTCACCATCAGATCTTTCCGCAGGCGGATCTGGGGTCGGCTGAACAGCTGCTGCTGGGCTGCGTTGA
CACGGCGCTGATGGCGCAAAATGCGCTGACGGCAGCCGAGTCGCTGGGGCTTGGCGGGGTATTCATTGGCGGCATACGCA
ATAATATTGAGCAGGTGACGGCGCTGCTGGGCTGCCCGCAGCATGTCCTGCCGCTAATGGGTCTGTGCTTGGGGCATCCG
GCGCAGCATCCGCAGCAAAAGCCGCGTCTGCCGCTGAGTATTCTGGTGCATGAGAATGGCTATCAGCTGCTGGATCGCGC
CGCGCTGGCGCAATACGACGCCCACGTTACTGCCTACTATGCCCAGCGTAGCGATAACGTCCGCCAAGAGAGCTGGAGCG
AACAGATGAGCCGTACCCTGGAGAAGGAGCAGCGTCCCTTTATGCTGGCCTACCTGCGGCGTCAGGGTTGGATCACCCAC
TGA

Upstream 100 bases:

>100_bases
AGCATTAAGGGGTAGACTGACTACCAGATTACGCCGTACCGTCAGCCATTGGTGCGGCCATTTCCGTCTCAAACGACTTA
CCGGGGAGCACTGTTTCGTT

Downstream 100 bases:

>100_bases
GCGCCGGCGCGCAGTGCTCAGTGGGCGCTGAACTGGTTGCTGTCGCGGTAGTAGCGGAAGCCGGCGGCGCCGAGGCGCGA
TTCCAGCAGGGGCTGTGGTA

Product: oxygen-insensitive NADPH nitroreductase

Products: NA

Alternate protein names: Modulator of drug activity A [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MMSTIDLLRDHRSIRQFSAEEIGEAQRLAILEAARATSSSSFLQCTSIIRITERALREQLVELAGGQQYVLDAAEFWVFC
ADFQRHHQIFPQADLGSAEQLLLGCVDTALMAQNALTAAESLGLGGVFIGGIRNNIEQVTALLGCPQHVLPLMGLCLGHP
AQHPQQKPRLPLSILVHENGYQLLDRAALAQYDAHVTAYYAQRSDNVRQESWSEQMSRTLEKEQRPFMLAYLRRQGWITH

Sequences:

>Translated_240_residues
MMSTIDLLRDHRSIRQFSAEEIGEAQRLAILEAARATSSSSFLQCTSIIRITERALREQLVELAGGQQYVLDAAEFWVFC
ADFQRHHQIFPQADLGSAEQLLLGCVDTALMAQNALTAAESLGLGGVFIGGIRNNIEQVTALLGCPQHVLPLMGLCLGHP
AQHPQQKPRLPLSILVHENGYQLLDRAALAQYDAHVTAYYAQRSDNVRQESWSEQMSRTLEKEQRPFMLAYLRRQGWITH
>Mature_240_residues
MMSTIDLLRDHRSIRQFSAEEIGEAQRLAILEAARATSSSSFLQCTSIIRITERALREQLVELAGGQQYVLDAAEFWVFC
ADFQRHHQIFPQADLGSAEQLLLGCVDTALMAQNALTAAESLGLGGVFIGGIRNNIEQVTALLGCPQHVLPLMGLCLGHP
AQHPQQKPRLPLSILVHENGYQLLDRAALAQYDAHVTAYYAQRSDNVRQESWSEQMSRTLEKEQRPFMLAYLRRQGWITH

Specific function: Reduction of nitroaromatic compounds using NADH. Reduces nitrofurazone by a ping-pong bi-bi mechanism possibly to generate a two-electron transfer product. Major component of the oxygen- insensitive nitroreductase activity in E.coli [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=239, Percent_Identity=68.2008368200837, Blast_Score=313, Evalue=4e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 26910; Mature: 26910

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSTIDLLRDHRSIRQFSAEEIGEAQRLAILEAARATSSSSFLQCTSIIRITERALREQL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
VELAGGQQYVLDAAEFWVFCADFQRHHQIFPQADLGSAEQLLLGCVDTALMAQNALTAAE
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SLGLGGVFIGGIRNNIEQVTALLGCPQHVLPLMGLCLGHPAQHPQQKPRLPLSILVHENG
HCCCCCEEECHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCC
YQLLDRAALAQYDAHVTAYYAQRSDNVRQESWSEQMSRTLEKEQRPFMLAYLRRQGWITH
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MMSTIDLLRDHRSIRQFSAEEIGEAQRLAILEAARATSSSSFLQCTSIIRITERALREQL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
VELAGGQQYVLDAAEFWVFCADFQRHHQIFPQADLGSAEQLLLGCVDTALMAQNALTAAE
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SLGLGGVFIGGIRNNIEQVTALLGCPQHVLPLMGLCLGHPAQHPQQKPRLPLSILVHENG
HCCCCCEEECHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCC
YQLLDRAALAQYDAHVTAYYAQRSDNVRQESWSEQMSRTLEKEQRPFMLAYLRRQGWITH
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7568050; 8755878; 8905232; 9278503; 2570347; 9440535; 11034992 [H]