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The map label for this gene is bcr [H]

Identifier: 238920400

GI number: 238920400

Start: 2410681

End: 2411928

Strand: Direct

Name: bcr [H]

Synonym: NT01EI_2510

Alternate gene names: 238920400

Gene position: 2410681-2411928 (Clockwise)

Preceding gene: 238920399

Following gene: 238920401

Centisome position: 63.23

GC content: 41.19

Gene sequence:

>1248_bases
ATGCTTACAACAAAAAATATGCCAGCCAATGCTGGGCTGGCAGCGTTTTCTGCTCAACAGAGAATCGCTTTCATATTAAT
CGTGTCCATATCTTTTTTGATGGGAATCAGCATCGACCTTTACGTACCATCGCTTCCCGCCATCTCACACTATTACCACT
CCACCATTGCGAGTGTCGAGATCAGTATTAGCCTATACTTGTTAGGTTATGGTCTCGGGCAAACAGTATTGGGTATTTTA
TCCGATAGCTATGGCCGAAAAAAAATATTCATTTACTCCTCGCTGTGTTTTTTATTATCCAGTATCTTCTGTGTCCTATC
AGACTCTAGCACCATGATGAACGGCTGGCGATTTGTGCAAGGTGTTGCGGTTGCAGGCTTAGCCTCTGGTATGAGGGCCA
TTATCGTCGACTTGTTTTCCGGCCTGACACTAAAGAAAATGGCTAACTATTTTGCGTTGAGTTGGGCATTAGGTCCTATC
ATCGCCCCCTGGATTGGCTCTAATCTGTCTCACTATTGTGGCTGGCAAGCTAACTTTTATTTATTTGCCGTTTATGGTGG
GGTAATATTTCTGATTTGCCTATTTCTTTTCCGAGAATCCAATCACAACCCCAGCAGATTCGAACTTAGCTCTATCAAGA
ATAATTTCATTGAGATTATCAGAAACAAGAAATTCACTCTATTAAGTATTATCTATGGCTTAAGTTATAGCTCAGTCTTA
GTTTTCAATACTGCTGGACCATATTTAATCGAAGTGGTCATGCACCATAGCGAGTTATTCTATGGCTATCTTGCCATGTT
CTTAGGATTAGCATATTTTGTTGGCACAATTATTAATAGGTACCTTATCACAAAATTTGATAACGAAGCCATTCTCAAAG
TGGGTGGGACCATGGCTCTCATCGTAGCCATATTGTTATGGCTTCTATTATCTTATGTGCCAAACATTTTTACCCTTATC
ATCCCCATATTTTTAATGTTTGTCTGTATTGGGCTTATTGTTCCTAACGCTATTGCCATTACCATGGTTATCTTTCCTGA
AAAAGCAGGTTTTGCCACATCGCTGTGTGGTACTATCGTGGGATTGGTCGTTTTTTTAGTGACTTATCTCATCGGCCACG
TCAAGATCTCTGCCCTGGAACTATCCCTGTGCTACTTCGCTCTTTTCCTGTTGCTCAATGTCATTAAACTGGCTGTCTAT
CATCAGAAAAACAGACCATCACCACCAGAGCATCTTCCCCCTTGCTAA

Upstream 100 bases:

>100_bases
TACCGCAGCTCGGTATCGATGGCAAGAGGATTACAGCCAAGCTGGTCTGGCAAAAACAGCCAACCCCATCCCTGTAAGAT
CAGACGATAAGGGTTCAGCC

Downstream 100 bases:

>100_bases
GCGGCTAGCCATGACAGCACACGACCGCGATGTGAAGCCAAAGGTGCAACATCGCGGCCACGTCTGACGCCTCAATAGCC
CCGTAACGCCCGGCACTCCC

Product: drug resistance transporter, Bcr/CflA family

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MLTTKNMPANAGLAAFSAQQRIAFILIVSISFLMGISIDLYVPSLPAISHYYHSTIASVEISISLYLLGYGLGQTVLGIL
SDSYGRKKIFIYSSLCFLLSSIFCVLSDSSTMMNGWRFVQGVAVAGLASGMRAIIVDLFSGLTLKKMANYFALSWALGPI
IAPWIGSNLSHYCGWQANFYLFAVYGGVIFLICLFLFRESNHNPSRFELSSIKNNFIEIIRNKKFTLLSIIYGLSYSSVL
VFNTAGPYLIEVVMHHSELFYGYLAMFLGLAYFVGTIINRYLITKFDNEAILKVGGTMALIVAILLWLLLSYVPNIFTLI
IPIFLMFVCIGLIVPNAIAITMVIFPEKAGFATSLCGTIVGLVVFLVTYLIGHVKISALELSLCYFALFLLLNVIKLAVY
HQKNRPSPPEHLPPC

Sequences:

>Translated_415_residues
MLTTKNMPANAGLAAFSAQQRIAFILIVSISFLMGISIDLYVPSLPAISHYYHSTIASVEISISLYLLGYGLGQTVLGIL
SDSYGRKKIFIYSSLCFLLSSIFCVLSDSSTMMNGWRFVQGVAVAGLASGMRAIIVDLFSGLTLKKMANYFALSWALGPI
IAPWIGSNLSHYCGWQANFYLFAVYGGVIFLICLFLFRESNHNPSRFELSSIKNNFIEIIRNKKFTLLSIIYGLSYSSVL
VFNTAGPYLIEVVMHHSELFYGYLAMFLGLAYFVGTIINRYLITKFDNEAILKVGGTMALIVAILLWLLLSYVPNIFTLI
IPIFLMFVCIGLIVPNAIAITMVIFPEKAGFATSLCGTIVGLVVFLVTYLIGHVKISALELSLCYFALFLLLNVIKLAVY
HQKNRPSPPEHLPPC
>Mature_415_residues
MLTTKNMPANAGLAAFSAQQRIAFILIVSISFLMGISIDLYVPSLPAISHYYHSTIASVEISISLYLLGYGLGQTVLGIL
SDSYGRKKIFIYSSLCFLLSSIFCVLSDSSTMMNGWRFVQGVAVAGLASGMRAIIVDLFSGLTLKKMANYFALSWALGPI
IAPWIGSNLSHYCGWQANFYLFAVYGGVIFLICLFLFRESNHNPSRFELSSIKNNFIEIIRNKKFTLLSIIYGLSYSSVL
VFNTAGPYLIEVVMHHSELFYGYLAMFLGLAYFVGTIINRYLITKFDNEAILKVGGTMALIVAILLWLLLSYVPNIFTLI
IPIFLMFVCIGLIVPNAIAITMVIFPEKAGFATSLCGTIVGLVVFLVTYLIGHVKISALELSLCYFALFLLLNVIKLAVY
HQKNRPSPPEHLPPC

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=351, Percent_Identity=26.2108262108262, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI48994889, Length=359, Percent_Identity=25.6267409470752, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1790146, Length=323, Percent_Identity=24.4582043343653, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87082312, Length=333, Percent_Identity=23.7237237237237, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1787065, Length=262, Percent_Identity=21.7557251908397, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790794, Length=219, Percent_Identity=25.1141552511416, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 45912; Mature: 45912

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTTKNMPANAGLAAFSAQQRIAFILIVSISFLMGISIDLYVPSLPAISHYYHSTIASVE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHEE
ISISLYLLGYGLGQTVLGILSDSYGRKKIFIYSSLCFLLSSIFCVLSDSSTMMNGWRFVQ
EEEEEEEEHHCHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
GVAVAGLASGMRAIIVDLFSGLTLKKMANYFALSWALGPIIAPWIGSNLSHYCGWQANFY
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHH
LFAVYGGVIFLICLFLFRESNHNPSRFELSSIKNNFIEIIRNKKFTLLSIIYGLSYSSVL
HHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEE
VFNTAGPYLIEVVMHHSELFYGYLAMFLGLAYFVGTIINRYLITKFDNEAILKVGGTMAL
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHH
IVAILLWLLLSYVPNIFTLIIPIFLMFVCIGLIVPNAIAITMVIFPEKAGFATSLCGTIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEEECCCCCHHHHHHHHHH
GLVVFLVTYLIGHVKISALELSLCYFALFLLLNVIKLAVYHQKNRPSPPEHLPPC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MLTTKNMPANAGLAAFSAQQRIAFILIVSISFLMGISIDLYVPSLPAISHYYHSTIASVE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHEE
ISISLYLLGYGLGQTVLGILSDSYGRKKIFIYSSLCFLLSSIFCVLSDSSTMMNGWRFVQ
EEEEEEEEHHCHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
GVAVAGLASGMRAIIVDLFSGLTLKKMANYFALSWALGPIIAPWIGSNLSHYCGWQANFY
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHH
LFAVYGGVIFLICLFLFRESNHNPSRFELSSIKNNFIEIIRNKKFTLLSIIYGLSYSSVL
HHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEE
VFNTAGPYLIEVVMHHSELFYGYLAMFLGLAYFVGTIINRYLITKFDNEAILKVGGTMAL
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHH
IVAILLWLLLSYVPNIFTLIIPIFLMFVCIGLIVPNAIAITMVIFPEKAGFATSLCGTIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEEECCCCCHHHHHHHHHH
GLVVFLVTYLIGHVKISALELSLCYFALFLLLNVIKLAVYHQKNRPSPPEHLPPC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]